Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,531,575 G→A 100% L542L (CTC→CTT RCAP_rcc02362 ← competence protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,531,5750G→A100.0% 867.2 221L542L (CTC→CTTRCAP_rcc02362competence protein
Reads supporting (aligned to +/- strand):  new base (106/115):  ref base (0/0):  total (106/115)

Only 100 of 263 total aligned reads displayed.

AGCGCCCGCCCCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACGAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGC  >  NC_014034/2531477‑2531671
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aGCCCCCGCCCCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCACGAGCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCAcaag                                                                                                <  1:4704083/101‑1 (MQ=255)
  cgcccgccCCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCAcaagcg                                                                                              <  2:2088361/101‑1 (MQ=255)
    cccgcccCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCAcaagcgcg                                                                                            <  1:3706032/101‑1 (MQ=255)
     ccgcccCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCCcaagcgcga                                                                                           >  1:3108651/1‑101 (MQ=255)
     ccgcccCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCAcaagcgcga                                                                                           >  1:2942418/1‑101 (MQ=255)
        ccccTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCAcaagcgcgagcg                                                                                        >  2:2236776/1‑101 (MQ=255)
           cTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCa                                                                                     <  2:459358/101‑1 (MQ=255)
           cTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCa                                                                                     <  1:3178578/101‑1 (MQ=255)
           cTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCa                                                                                     >  2:5018566/1‑101 (MQ=255)
            tCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAg                                                                                    <  1:1333569/101‑1 (MQ=255)
            tCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAg                                                                                    >  1:3573410/1‑101 (MQ=255)
             cGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGc                                                                                   >  2:3125872/1‑101 (MQ=255)
             cGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGc                                                                                   <  2:1333793/101‑1 (MQ=255)
             cGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGc                                                                                   >  1:5412219/1‑101 (MQ=255)
              gggCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCa                                                                                  <  2:1958330/101‑1 (MQ=255)
              gggCGTCATAAGCCCGGCAAGCGCGCCCTCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCa                                                                                  <  1:1998567/101‑1 (MQ=255)
                gCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATc                                                                                <  2:5099593/101‑1 (MQ=255)
                gCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATc                                                                                <  1:5185828/101‑1 (MQ=255)
                  gTCATCAGCCCGACAAGCGCGCCCCCGGGCTCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcgc                                                                              <  2:3904575/101‑1 (MQ=255)
                  gTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGAGAGCGCCAGCATcgc                                                                              >  1:4612347/1‑101 (MQ=255)
                   tcatcaGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCATCGCCGGACGGGGGGGAAGCCCCCAGCCCGCAAAAGCCACAACCGCGAGCGCCACCAAcgcc                                                                             >  1:2709787/1‑101 (MQ=255)
                   tcatcaGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcgcc                                                                             >  2:5602559/1‑101 (MQ=255)
                   ccatcaGCCCGACAAGCGCGCCCCCGGGCGCCATCAGCAGCCCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcgcc                                                                             <  1:1476944/100‑1 (MQ=255)
                    cagcaGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcgccg                                                                            <  2:3504845/98‑1 (MQ=255)
                      tcaGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCcgcg                                                                          <  2:4994527/101‑1 (MQ=255)
                        aGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCCGCCCGCAAAGGCCACACGCGCGAGCGCCAGCATCGCcgcgcg                                                                        >  1:4630068/1‑101 (MQ=255)
                          cccGCCCAGCGCGCCCCCGGGCCCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGa                                                                      <  2:2728664/101‑1 (MQ=255)
                          cccGACCAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGa                                                                      >  2:5764028/1‑101 (MQ=255)
                            cGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAAc                                                                    >  1:2813878/1‑101 (MQ=255)
                             gACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAAcg                                                                   <  1:805140/101‑1 (MQ=255)
                             gACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAAcg                                                                   >  1:3241643/1‑101 (MQ=255)
                             gACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAAcg                                                                   >  1:3041261/1‑101 (MQ=255)
                               cAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAAcgcg                                                                 >  2:2309058/1‑101 (MQ=255)
                                 aGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGcc                                                               <  2:4122385/101‑1 (MQ=255)
                                 aGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGcc                                                               >  1:2441301/1‑101 (MQ=255)
                                   ctcgcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGcccc                                                             <  2:891431/99‑1 (MQ=255)
                                    gcgcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCcg                                                            >  1:4408725/1‑101 (MQ=255)
                                    gcgcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCcg                                                            <  1:4538444/101‑1 (MQ=255)
                                     cgcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCcgc                                                           <  1:2309184/101‑1 (MQ=255)
                                      gcCCCCGGGCGAGATTAGCAGCGGCGGCCGGGGGCCATGCCCCCAGCCCGCAAAGGCCAAAAGCGCGAGCGCCAGCATCCCCGCGCGGAACGCGCCCcgcg                                                          <  2:5233915/101‑1 (MQ=255)
                                        ccccGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGAc                                                        >  1:169212/1‑101 (MQ=255)
                                         cccGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCTCCGCGCGGAACGCGCCCCGCGACa                                                       <  1:5777454/101‑1 (MQ=255)
                                           cGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGc                                                     <  1:3842619/101‑1 (MQ=255)
                                            gggCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCa                                                    <  2:2503297/101‑1 (MQ=255)
                                            gggCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCa                                                    >  1:1268892/1‑101 (MQ=255)
                                             ggCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAc                                                   <  1:4155721/101‑1 (MQ=255)
                                             ggCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAc                                                   >  2:4445420/1‑101 (MQ=255)
                                              gcgcGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAcc                                                  <  2:1495153/101‑1 (MQ=255)
                                                 cgATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAccgcc                                               <  1:5576172/101‑1 (MQ=255)
                                                 cgATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAccgcc                                               <  1:2240871/101‑1 (MQ=255)
                                                 cgATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAccgcc                                               <  1:1592574/101‑1 (MQ=255)
                                                  gATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAccgccg                                              >  2:3297798/1‑101 (MQ=255)
                                                  gATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCACGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAccgccg                                              <  2:1875140/101‑1 (MQ=255)
                                                     cagcagCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGccc                                           <  2:3305911/101‑1 (MQ=255)
                                                     cagcagCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGccc                                           >  2:3739692/1‑101 (MQ=255)
                                                      agcagcGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGcccc                                          <  2:1278239/101‑1 (MQ=255)
                                                       gcagcGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCg                                         >  2:3989802/1‑101 (MQ=255)
                                                       gcagcGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCg                                         >  2:5429756/1‑101 (MQ=255)
                                                        cagcGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCgg                                        <  2:5569678/101‑1 (MQ=255)
                                                         agcGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGc                                       >  2:2410915/1‑101 (MQ=255)
                                                          gcgcCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGcc                                      <  1:5623727/101‑1 (MQ=255)
                                                           cgcCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCg                                     <  1:5733435/101‑1 (MQ=255)
                                                             ccGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCa                                   >  1:2542239/1‑101 (MQ=255)
                                                              cGGGCGGAGCGCAAGCCCCCAGCCCGCAAAGGCAACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCACCGCGACAGCACCGCCGCCCCGGCCGCaa                                  <  2:1098731/101‑1 (MQ=255)
                                                               ggACGGGGTGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCTCGGCCGCAAg                                 <  1:5000702/101‑1 (MQ=255)
                                                               ggACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAg                                 >  2:2189715/1‑101 (MQ=255)
                                                                gACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAgc                                <  2:4501534/101‑1 (MQ=255)
                                                                  cGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAgcgc                              >  2:2989274/1‑101 (MQ=255)
                                                                   ggggggCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGcc                             <  2:810563/101‑1 (MQ=255)
                                                                   ggggggCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGcc                             >  1:1003320/1‑101 (MQ=255)
                                                                   gggggcCAACCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGcc                             <  2:3973102/101‑1 (MQ=255)
                                                                    gggggCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCGGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCa                            >  1:4932379/1‑101 (MQ=255)
                                                                          aaGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCg                      >  1:3857343/1‑101 (MQ=255)
                                                                          aaGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCg                      >  1:4020595/1‑101 (MQ=255)
                                                                          aaGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCg                      >  1:4855814/1‑101 (MQ=255)
                                                                           aGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCgg                     >  2:2302103/1‑101 (MQ=255)
                                                                             cccccAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGgca                   <  1:2179104/101‑1 (MQ=255)
                                                                              ccccAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCCCAAGCACCGgcac                  >  2:1692183/1‑100 (MQ=255)
                                                                               cccAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGggagc                 >  1:1448930/1‑101 (MQ=255)
                                                                                 cAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCAc               <  1:936191/101‑1 (MQ=255)
                                                                                 cAGCCCGCAAAGGCCACAAGCGCGAGCCCCAGCATCGCCGCGCGGAACCCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCAc               <  1:1812418/101‑1 (MQ=255)
                                                                                     ccGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAg           <  2:3428700/101‑1 (MQ=255)
                                                                                      cGCTAAGGCCACAAGCGCGAGCGCCAGCTTCCCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGc          <  1:5083059/101‑1 (MQ=255)
                                                                                       gCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGcc         <  2:3187070/101‑1 (MQ=255)
                                                                                        cAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCg        >  2:2538798/1‑101 (MQ=255)
                                                                                        cAAAGCCCACAAGCGCGCGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGTCCGCAAGCGCCAGCACCGGCAGCACCCAGCCg        <  2:3241443/101‑1 (MQ=255)
                                                                                         aaaGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCgg       >  2:619586/1‑101 (MQ=255)
                                                                                         aaaGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCgg       >  1:1011194/1‑101 (MQ=255)
                                                                                         aaaGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCgg       >  1:5456935/1‑101 (MQ=255)
                                                                                         aaaGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCgg       >  1:1778639/1‑101 (MQ=255)
                                                                                         aaaGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCCCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCgg       <  2:5525283/101‑1 (MQ=255)
                                                                                         aaaGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCCCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCgg       >  2:5729415/1‑101 (MQ=255)
                                                                                          atggCCCCAAGCGCGAGCCCCAGCACCGCTGCGCGGAACGCGCGTCGCGTCAGCGCCGCGGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCggg      <  2:4826376/99‑1 (MQ=255)
                                                                                           aGGCCACAAGGGCGCGCGCCACCACCCCCGCGTGGGACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGggc     <  2:2768797/101‑1 (MQ=255)
                                                                                             gCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGggcgg   <  1:2656984/101‑1 (MQ=255)
                                                                                             gCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGggcgg   <  2:4300825/101‑1 (MQ=255)
                                                                                              ccACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGggcggc  <  2:56730/101‑1 (MQ=255)
                                                                                              ccACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGggcggc  <  1:1113579/101‑1 (MQ=255)
                                                                                              ccACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGggcggc  <  2:3585709/101‑1 (MQ=255)
                                                                                              ccACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGggcggc  >  2:3719252/1‑101 (MQ=255)
                                                                                                  |                                                                                                
AGCGCCCGCCCCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACGAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGC  >  NC_014034/2531477‑2531671

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG