Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,210 T→G 15.1% intergenic (‑186/+90) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2100T→G15.1% 81.8 ‑1.2 ‑0.5199intergenic (‑186/+90)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/15):  ref base (102/66):  total (117/82)
Fisher's exact test strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.42e-01

Only 100 of 211 total aligned reads displayed.

CAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGA  >  NC_014034/2640110‑2640310
                                                                                                      |                                                                                                    
   ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                                                   <  2:4538823/101‑1 (MQ=25)
       ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                                                                               <  2:535389/101‑1 (MQ=25)
         cgcTTTTGCGCAGGCAAAAGGGGGGGGCTGCCCCCCCCTT.GGCCTTCGGCCAATTTACCCCCCCCGGGATTTTGAGCAAGATG.AAAGACCATGGGTTTAtt                                                                                             >  1:881576/1‑100 (MQ=255)
                gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                                      <  2:4110343/101‑1 (MQ=25)
                 cccccccgaaaAGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCaa                                                                                    >  1:3307166/9‑101 (MQ=9)
                   cacgCAAAAGGGGGGCGCTGCCCCCCTCTTGGG.CTTCGGCCCATTTACCCCCCGAGGGATTTGGGGCAAAATG.AAAGACCCTGGGTTTATTTTGCCTCaaa                                                                                   >  1:3859305/4‑101 (MQ=16)
                     cccgaaaAGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                                                >  1:3613185/5‑101 (MQ=17)
                      gCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGGGCCAGATG.AAAGACCATGGGGTTATCTTTCCTCCAatat                                                                                >  1:3396239/1‑101 (MQ=17)
                      gCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                                                <  1:1847421/101‑1 (MQ=21)
                        gaaaAGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                             <  1:3834882/100‑1 (MQ=17)
                         aaaAGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                            <  2:24664/101‑1 (MQ=17)
                         aaaAGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                            >  2:4791669/1‑101 (MQ=17)
                         aaaAGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                            <  1:4119471/101‑1 (MQ=17)
                          aaGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCg                                                                            <  2:4554574/101‑1 (MQ=25)
                           aagggggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                                          >  1:1867500/3‑101 (MQ=18)
                           aagggggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                                          >  1:1167496/3‑101 (MQ=18)
                            ggggggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                                          >  1:5782600/1‑101 (MQ=21)
                             gggggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                                        >  1:3540700/1‑101 (MQ=18)
                             gggggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                                        <  1:2358007/101‑1 (MQ=18)
                             gggggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                                        >  2:3177246/1‑101 (MQ=18)
                             gggggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                                                                         >  2:4815310/1‑101 (MQ=25)
                              ggggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                                                       <  1:2005554/101‑2 (MQ=18)
                               gggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCTCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCcggggtg                                                                      <  2:164463/101‑3 (MQ=18)
                                ggCGCTGCCCCCCTCTTGGG.CTTCGGCCAAGTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                                      <  1:4327505/101‑1 (MQ=25)
                                ggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                                     >  2:1178424/1‑98 (MQ=18)
                                 gcgcTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                                    <  2:3114982/101‑5 (MQ=21)
                                  cgcCGCCCCCCTC.G.GG.CTTC.GCCAA.TCGCCCCCCGGGATATTTCGGGCAAGATGAAAGGCATCCGGTTTCATCTTGCTGCAAATATCCCGGGGGGAGTccg                                                                 <  2:2060213/101‑2 (MQ=17)
                                   gcTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgcgggtgaggc                                                                  >  2:4322320/1‑95 (MQ=21)
                                   gcTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                                   >  2:2350610/1‑101 (MQ=25)
                                     tGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggggaggcga                                                                >  1:1368990/1‑94 (MQ=21)
                                     tGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                                 >  2:684030/1‑101 (MQ=25)
                                      gCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCCCGGTATTTTGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGCGGGGAGTCCtc                                                                >  2:3333491/1‑100 (MQ=25)
                                      gCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCCAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCCGGGGGAGGCCTg                                                                >  2:609878/1‑101 (MQ=25)
                                       ccccccTCTT.GGCCTTTGGCCAATTCACCCCCCGAGGTATTTGGAGCCAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCtta                                                               >  1:1424258/1‑99 (MQ=25)
                                          cccTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                            >  2:4566733/1‑101 (MQ=37)
                                            ctctT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTTAAAGGa                                                          >  1:952612/1‑101 (MQ=25)
                                            ctctT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                                          >  2:4410668/1‑101 (MQ=37)
                                             tctT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                         <  2:5167840/101‑1 (MQ=37)
                                                ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                                       >  2:2135986/6‑101 (MQ=37)
                                                ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                                       <  1:1039159/96‑1 (MQ=37)
                                                    ccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                                   <  2:5782305/100‑1 (MQ=37)
                                                     cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                                  <  1:3232990/101‑1 (MQ=255)
                                                       tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                                >  2:112307/1‑101 (MQ=255)
                                                        cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                               <  1:1533075/101‑1 (MQ=255)
                                                        cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                               >  1:83945/1‑101 (MQ=255)
                                                         ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                              >  1:5661350/1‑101 (MQ=255)
                                                            cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                                           >  1:29023/1‑101 (MQ=37)
                                                             aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                          >  2:910250/1‑101 (MQ=37)
                                                             aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                          >  1:3717657/1‑101 (MQ=37)
                                                              aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                         >  2:2729966/1‑101 (MQ=255)
                                                               ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                        >  1:4694849/1‑101 (MQ=25)
                                                                tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                       >  1:2473088/4‑101 (MQ=255)
                                                                tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                       <  1:4815591/98‑1 (MQ=255)
                                                                 caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                      >  1:4102655/3‑101 (MQ=255)
                                                                 caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                      >  2:3078918/3‑101 (MQ=255)
                                                                  accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                     >  2:5580576/2‑101 (MQ=255)
                                                                  accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                     <  1:4397032/100‑1 (MQ=255)
                                                                   ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                                    <  1:176/101‑1 (MQ=255)
                                                                    cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                                   <  1:4054548/101‑1 (MQ=255)
                                                                    cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                                   >  2:4060306/1‑101 (MQ=255)
                                                                     ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                                  >  2:3234337/1‑101 (MQ=255)
                                                                       ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCGCGAAAGGACGGGGGGCAGAGGCCCCCTCCCGGGt                                >  1:4970158/1‑101 (MQ=25)
                                                                       ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                                <  1:587100/101‑1 (MQ=255)
                                                                        cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                               >  1:1931677/1‑101 (MQ=255)
                                                                         gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                              <  1:4441108/101‑1 (MQ=255)
                                                                         gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                              >  1:3826016/1‑101 (MQ=255)
                                                                          aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                             >  1:160650/1‑101 (MQ=255)
                                                                            gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc                           <  2:1569887/101‑1 (MQ=255)
                                                                             tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                          >  1:2113747/1‑101 (MQ=255)
                                                                             tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                          >  2:857877/1‑101 (MQ=255)
                                                                             tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGAGGAGTCCTGAAAGGACGGGGGGCAGAGCACCCCTCCCGGGTCTTtct                          >  2:4993926/1‑101 (MQ=37)
                                                                               tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa                        <  1:1477560/101‑1 (MQ=255)
                                                                               tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa                        <  2:3717429/101‑1 (MQ=255)
                                                                               tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa                        <  1:4170348/101‑1 (MQ=255)
                                                                                ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                       <  1:4567009/101‑1 (MQ=255)
                                                                                 tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg                      <  2:3892420/101‑1 (MQ=255)
                                                                                  ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                     >  1:2249088/1‑101 (MQ=255)
                                                                                      cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                 >  2:3863652/1‑101 (MQ=255)
                                                                                       aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCgagat                >  2:3414713/1‑97 (MQ=255)
                                                                                       aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc                >  2:3592181/1‑101 (MQ=255)
                                                                                       aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc                >  1:5376445/1‑101 (MQ=255)
                                                                                       aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCCTTTCTCAAGCGTTcc                >  2:1409480/1‑101 (MQ=255)
                                                                                        aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg               <  1:2994544/101‑1 (MQ=255)
                                                                                          gatgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCgg              >  1:3364800/5‑101 (MQ=255)
                                                                                           atgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc             >  1:700097/4‑101 (MQ=255)
                                                                                           atgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc             >  1:3964498/4‑101 (MQ=255)
                                                                                           atgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc             >  1:2167755/4‑101 (MQ=255)
                                                                                            tgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCt            >  1:1597662/3‑101 (MQ=255)
                                                                                              aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa          >  2:4093266/1‑101 (MQ=255)
                                                                                              aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa          >  2:1857935/1‑101 (MQ=255)
                                                                                               aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg         >  2:4224574/1‑101 (MQ=255)
                                                                                               aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCAGGGTCTTTCTCAAGCGTTCCGGCTCAg         >  2:710083/1‑101 (MQ=255)
                                                                                               aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGCAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg         >  2:793467/1‑101 (MQ=255)
                                                                                                aGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGa        <  2:3396034/101‑1 (MQ=255)
                                                                                                aGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGa        >  2:2196955/1‑101 (MQ=255)
                                                                                                  aCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc      <  1:1223911/101‑1 (MQ=255)
                                                                                                    cATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCg    >  2:4667081/1‑101 (MQ=255)
                                                                                                    cATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCg    <  2:3672014/101‑1 (MQ=255)
                                                                                                    cATGGGATCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCg    >  1:1283049/1‑101 (MQ=255)
                                                                                                      tGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGa  <  2:1765947/101‑1 (MQ=255)
                                                                                                      |                                                                                                    
CAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGA  >  NC_014034/2640110‑2640310

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG