Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,715,979 A→G 100% F8L (TTC→CTC)  amaB ← N‑carbamoyl‑L‑amino acid amidohydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,715,9790A→G100.0% 1177.6 312F8L (TTC→CTC) amaBN‑carbamoyl‑L‑amino acid amidohydrolase
Reads supporting (aligned to +/- strand):  new base (151/161):  ref base (0/0):  total (151/161)

Only 100 of 315 total aligned reads displayed.

ATCGGTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAAGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAAGAG  >  NC_014034/2715881‑2716076
                                                                                                  |                                                                                                 
atcggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGt                                                                                                 >  1:931273/1‑101 (MQ=255)
   ggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGtttt                                                                                              >  2:1728769/1‑101 (MQ=255)
   ggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGtttt                                                                                              <  2:2317337/101‑1 (MQ=255)
    gTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTtc                                                                                             >  1:4873161/1‑101 (MQ=255)
    gTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTtc                                                                                             >  1:2556367/1‑101 (MQ=255)
     tCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTtct                                                                                            >  2:157957/1‑101 (MQ=255)
      cACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTtctc                                                                                           >  1:5694243/1‑101 (MQ=255)
      cACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTtctc                                                                                           <  1:185644/101‑1 (MQ=255)
       aCGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTcc                                                                                          >  2:291360/1‑101 (MQ=255)
       aCGGTCTGGCGGTTGTTG.CCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCg                                                                                         >  1:2954437/1‑101 (MQ=255)
        cGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCg                                                                                         <  2:1818814/101‑1 (MQ=255)
         ggTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCgg                                                                                        >  1:327911/1‑101 (MQ=255)
          gTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCggg                                                                                       >  1:629041/1‑101 (MQ=255)
          gTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCggg                                                                                       <  1:3869730/101‑1 (MQ=255)
          gTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGATTTCTCCggg                                                                                       >  2:890040/1‑101 (MQ=255)
                cGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATgg                                                                                 >  1:2611898/1‑101 (MQ=255)
                 ggTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGc                                                                                <  1:1359188/101‑1 (MQ=255)
                   ttgttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCAt                                                                              <  2:503055/101‑1 (MQ=255)
                   ttgttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCAt                                                                              <  1:3023747/101‑1 (MQ=255)
                     gttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCt                                                                            <  1:1605150/101‑1 (MQ=255)
                     gttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCt                                                                            >  2:4569091/1‑101 (MQ=255)
                     gttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCt                                                                            <  1:2174752/101‑1 (MQ=255)
                     gttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCt                                                                            >  2:4056491/1‑101 (MQ=255)
                      ttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTg                                                                           <  2:2565653/101‑1 (MQ=255)
                      ttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTg                                                                           <  2:3937142/101‑1 (MQ=255)
                       tgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTgg                                                                          >  2:4946333/1‑101 (MQ=255)
                        gCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGc                                                                         >  2:2024932/1‑101 (MQ=255)
                        gCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGc                                                                         <  2:4716401/101‑1 (MQ=255)
                        gCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGc                                                                         <  2:2618564/101‑1 (MQ=255)
                        gCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGc                                                                         >  1:1101677/1‑101 (MQ=255)
                        cccccccGCGATCCCCTGCCCGCCCGGCGCCATCTGCATGAGTCTGTCCCACAGGCCCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTggt                                                                         <  1:4320135/100‑2 (MQ=255)
                         ccccccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCt                                                                        >  2:1322305/1‑101 (MQ=255)
                         ccccccGCGATCCCCGGCCCCACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCt                                                                        >  2:353440/1‑101 (MQ=255)
                          cccccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTg                                                                       <  2:4319014/101‑1 (MQ=255)
                            cccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGtc                                                                     <  1:4107857/101‑1 (MQ=255)
                             ccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCGGtct                                                                    >  2:3652954/1‑101 (MQ=255)
                               gcgATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTcc                                                                  <  1:45637/101‑1 (MQ=255)
                               gagttCCCCGGCCCGACCTACGCCATCTGCATGAGTCTGTCCCCCAGGCGCGCGAAATTGATAGGGAGGTTTTTTCCGGGTGATGGCATCTGGCTGTCTcc                                                                  <  2:2805895/97‑1 (MQ=255)
                                 gATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCt                                                                <  1:1499/101‑1 (MQ=255)
                                   tCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgt                                                              >  1:4385076/1‑101 (MQ=255)
                                    ccccGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgtc                                                             <  1:739013/101‑1 (MQ=255)
                                     cccGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgtcg                                                            <  2:691354/101‑1 (MQ=255)
                                     cccGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgtcg                                                            <  1:274258/101‑1 (MQ=255)
                                       cGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgtcgtc                                                          <  1:5782054/101‑1 (MQ=255)
                                         gcacGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGCTACGGAGGTTTTCTCCGGCGGATGGCATCTGGCTGTCTCCCTGTCGTCTg                                                        >  2:1838755/4‑101 (MQ=255)
                                         gCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTg                                                        >  2:1233577/1‑101 (MQ=255)
                                          cccGACCTGCGCCAGCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGTTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGc                                                       <  2:3793438/101‑1 (MQ=255)
                                           ccGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCg                                                      <  2:762073/101‑1 (MQ=255)
                                            cGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCgg                                                     >  2:4788408/1‑101 (MQ=255)
                                             gACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCggg                                                    >  1:4713250/1‑101 (MQ=255)
                                               ccTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGc                                                  <  2:560281/101‑1 (MQ=255)
                                                 tGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCtt                                                <  2:1383008/101‑1 (MQ=255)
                                                   cgcCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTg                                              <  1:3219296/101‑1 (MQ=255)
                                                    gcCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGc                                             <  1:5024754/101‑1 (MQ=255)
                                                     ccATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGcc                                            >  1:2788576/1‑101 (MQ=255)
                                                       aTCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTTGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCct                                          >  1:5005609/1‑101 (MQ=255)
                                                       aTCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCct                                          >  1:4536905/1‑101 (MQ=255)
                                                       aTCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCct                                          >  2:5374528/1‑101 (MQ=255)
                                                        tCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCctc                                         <  1:5175975/101‑1 (MQ=255)
                                                         cTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCctct                                        <  1:3149561/101‑1 (MQ=255)
                                                         cTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCctct                                        >  1:3324316/1‑101 (MQ=255)
                                                           gCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTg                                      <  2:2206020/101‑1 (MQ=255)
                                                           ccgatctgCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTg                                      <  2:5003756/94‑1 (MQ=255)
                                                             aTGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCg                                    >  1:3725321/1‑101 (MQ=255)
                                                               gAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGAc                                  >  2:4013810/1‑101 (MQ=255)
                                                                aGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACt                                 >  2:3091169/1‑101 (MQ=255)
                                                                  gCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCa                               >  2:2152204/1‑101 (MQ=255)
                                                                  gCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCa                               >  1:4015694/1‑101 (MQ=255)
                                                                  gCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCa                               >  2:5184930/1‑101 (MQ=255)
                                                                    tGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAtt                             <  1:5374818/101‑1 (MQ=255)
                                                                    tGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAtt                             >  1:5516198/1‑101 (MQ=255)
                                                                     gTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAttt                            <  2:1077038/101‑1 (MQ=255)
                                                                      tCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAtttt                           <  1:4920085/101‑1 (MQ=255)
                                                                       cccACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTc                          <  2:5138041/101‑1 (MQ=255)
                                                                        ccACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCt                         <  2:327867/101‑1 (MQ=255)
                                                                        ccACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCt                         <  1:3447602/101‑1 (MQ=255)
                                                                         cacaGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTg                        <  2:4677653/101‑1 (MQ=255)
                                                                              gcgcgcGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCgt                   >  1:4610137/1‑100 (MQ=255)
                                                                                gcgcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGAtt                 >  1:4507242/1‑101 (MQ=255)
                                                                                  gcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTgg               <  2:1172605/101‑1 (MQ=255)
                                                                                  gcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTgg               <  1:1728868/101‑1 (MQ=255)
                                                                                   cgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGt              >  1:5322182/1‑101 (MQ=255)
                                                                                   cgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGt              >  1:5414477/1‑101 (MQ=255)
                                                                                    ggAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTc             >  1:3782059/1‑101 (MQ=255)
                                                                                     gAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCa            >  2:5173483/1‑101 (MQ=255)
                                                                                     gAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCa            >  2:3201462/1‑101 (MQ=255)
                                                                                      aaTTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCag           <  2:4417092/101‑1 (MQ=255)
                                                                                        ttGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCagag         >  1:4287496/1‑101 (MQ=255)
                                                                                        ttGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCagag         <  1:4788689/101‑1 (MQ=255)
                                                                                         tGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAgg        >  1:2390673/1‑101 (MQ=255)
                                                                                         tGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAgg        >  1:2133549/1‑101 (MQ=255)
                                                                                          gATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGa       >  1:4250376/1‑101 (MQ=255)
                                                                                           aTACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGaa      <  1:1262008/101‑1 (MQ=255)
                                                                                           aTACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGaa      <  1:4891459/101‑1 (MQ=255)
                                                                                            tACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGaaa     <  2:2481283/101‑1 (MQ=255)
                                                                                            tACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGaaa     <  1:3384378/101‑1 (MQ=255)
                                                                                             aCGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAag    <  2:298574/101‑1 (MQ=255)
                                                                                             aCGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAag    >  2:614712/1‑101 (MQ=255)
                                                                                              cGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCGGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAaga   >  2:5001216/1‑101 (MQ=255)
                                                                                               ggAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAagag  >  2:5534955/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
ATCGGTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAAGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAAGAG  >  NC_014034/2715881‑2716076

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG