Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,876,561 G→A 2.1% intergenic (+60/‑133) RCAP_rcc02685 → / → prfA glutathione S‑transferase/peptide chain release factor 1

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,876,5610G→A 2.1% 3.1 ‑4.0 ‑0.1192intergenic (+60/‑133)RCAP_rcc02685/prfAglutathione S‑transferase/peptide chain release factor 1
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (68/120):  total (72/120)
Fisher's exact test strand distribution p-value = 1.87e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.26e-01

Only 100 of 211 total aligned reads displayed.

CGCGACCTGATGGAAAGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGAAATCCCCGCCCACCCCCGCCCACCCT  >  NC_014034/2876463‑2876660
                                                                                                  |                                                                                                      
cgcgACCTGATGGAAAGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGcc                                                                                                      <  1:2651243/101‑1 (MQ=255)
 gcgACCTGATGGAAAGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCa                                                                                                     <  2:5626963/101‑1 (MQ=255)
   gACCTGATGGAAAGACGCGCTGCCGAACCGTCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATc                                                                                                   <  1:371339/101‑1 (MQ=255)
   gACCTGATGGAAAGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATc                                                                                                   <  2:5184438/101‑1 (MQ=255)
   gACCTGATGGAAAGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAACACGCCGGGGGTCGTCCATTGCGGCGCCATc                                                                                                   >  1:256069/1‑101 (MQ=255)
    aCCTGATGGAAAGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCg                                                                                                  >  1:4488949/1‑101 (MQ=255)
     ccTGATGGAAAGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCgg                                                                                                 <  2:4682730/101‑1 (MQ=255)
     ccTGATGGAAAGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCgg                                                                                                 >  1:3050190/1‑101 (MQ=255)
     ccTGATGGAAAGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGGCGTCCATTGCGGCGCCATCgg                                                                                                 >  1:2091701/1‑101 (MQ=255)
      cTGATGGAAAGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGt                                                                                                <  1:2929242/101‑1 (MQ=255)
       tGATGGAAAGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGtt                                                                                               <  1:884596/101‑1 (MQ=255)
       tGATGGAAAGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGtt                                                                                               <  1:1998298/101‑1 (MQ=255)
        gATGGAAAGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTc                                                                                              >  1:1866347/1‑101 (MQ=255)
          tGGAAAGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCaa                                                                                            <  2:565356/101‑1 (MQ=255)
           gggaaGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAg                                                                                           <  2:2737851/98‑1 (MQ=255)
             aaaGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.c.c                                                                                       <  2:3668567/101‑1 (MQ=255)
             aaaGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGACATCGGTTCAAG.c.c                                                                                       <  2:4315840/101‑1 (MQ=255)
               aGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCg                                                                                     >  1:5323952/1‑101 (MQ=255)
                gACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGa                                                                                    <  2:496347/101‑1 (MQ=255)
                 aCGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGAt                                                                                   >  2:4812434/1‑101 (MQ=255)
                  cgcgCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATg                                                                                  >  1:2616885/1‑101 (MQ=255)
                  cgcgCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATg                                                                                  <  1:2800928/101‑1 (MQ=255)
                   gcgcTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATgg                                                                                 <  1:3309094/101‑1 (MQ=255)
                   gcgcTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATgg                                                                                 <  1:2196406/101‑1 (MQ=255)
                    cgcTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGga                                                                                <  1:3636335/101‑1 (MQ=255)
                       tGCCGGACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGgacga                                                                             <  1:2833890/101‑1 (MQ=255)
                        gCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGgacgac                                                                            <  2:3139452/101‑1 (MQ=255)
                        gCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGgacgac                                                                            <  1:1746609/101‑1 (MQ=255)
                          cGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACgg                                                                          <  1:4769569/101‑1 (MQ=255)
                           gAACCGGCGTGATCCGCCGCGCGCCCCTGATTTTTCTTGCGTAAAATTCTCCTTGCGCCGTCCATTACGGAGCCATC.GATCAAT.CTCAGATGGACGACggg                                                                         >  2:929869/1‑101 (MQ=255)
                            aaCCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACgggg                                                                        <  2:2399630/101‑1 (MQ=255)
                            aaCCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACgggg                                                                        <  1:4782150/101‑1 (MQ=255)
                             aCCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGc                                                                       <  2:3203598/101‑1 (MQ=255)
                             aCCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGc                                                                       <  1:1477995/101‑1 (MQ=255)
                              ccGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCa                                                                      >  1:5365433/1‑101 (MQ=255)
                              ccGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCa                                                                      <  1:1901243/101‑1 (MQ=255)
                               cGGCGTAAACCGGCGCCCGCCTGTGCTTTTTCGTGGGGAAAAGACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAg                                                                     <  2:3792179/101‑1 (MQ=25)
                                ggCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAgc                                                                    >  1:1369539/1‑101 (MQ=255)
                                ggCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAACACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAgc                                                                    <  2:256036/101‑1 (MQ=255)
                                ggCGTGATCCCCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGACGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAgc                                                                    <  1:4869877/101‑1 (MQ=255)
                                 tcttGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAgcg                                                                   <  2:2257053/98‑1 (MQ=255)
                                  cGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAgcgc                                                                  >  2:3464795/1‑101 (MQ=255)
                                   gTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGcc                                                                 <  1:1316527/101‑1 (MQ=255)
                                    tGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGccc                                                                <  1:1511542/101‑1 (MQ=255)
                                      aTCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGccccc                                                              <  2:1492620/101‑1 (MQ=255)
                                      aTCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGccccc                                                              <  1:4313460/101‑1 (MQ=255)
                                      aTCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGGCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGccccc                                                              <  1:757613/101‑1 (MQ=255)
                                       tCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCcccg                                                             <  1:5626628/101‑1 (MQ=255)
                                          gccgcGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCcccgccc                                                          >  1:2700019/1‑101 (MQ=255)
                                          gccgcGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCcccgccc                                                          <  2:2054083/101‑1 (MQ=255)
                                           ccgcGCGCCCCTGCTTTTTCTTGGGGAAAATCCGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCcccgcccg                                                         <  1:3992620/101‑1 (MQ=255)
                                             gcgcgcCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCggg                                                       <  1:227988/101‑1 (MQ=255)
                                              cgcgcCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.ACGATGGACGACGGGGCAGCGCCCCCGCCCGGGt                                                      <  2:2592677/101‑1 (MQ=255)
                                               gcgcCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTc                                                     <  2:1049278/101‑1 (MQ=255)
                                                 gcCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGc                                                   >  2:1187739/1‑101 (MQ=255)
                                                 gcCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGc                                                   >  1:1912046/1‑101 (MQ=255)
                                                   cccTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCCCCCCGGGCGccc                                                 >  1:4154959/1‑101 (MQ=255)
                                                   cccTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATCGACGACGGGGCAGCGCCCCCCCCCCGGGCGccc                                                 >  1:5020184/1‑101 (MQ=37)
                                                    ccTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGcccc                                                <  1:38431/101‑1 (MQ=255)
                                                      tGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCNCGCCCGCGTCGCCCCgg                                              >  2:2819740/1‑101 (MQ=255)
                                                       gCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCCCCCCGGGCGCCCCcgg                                             >  2:1298797/1‑101 (MQ=37)
                                                        cTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGgc                                            <  2:4229924/101‑1 (MQ=255)
                                                        cTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGgc                                            >  1:391177/1‑101 (MQ=255)
                                                        cTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCCCCCCGGGCGCCCCcggg                                            >  1:4641579/1‑100 (MQ=38)
                                                         tttttCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCCCCCCGGGCGCCCCcgggg                                           >  1:5428641/1‑99 (MQ=37)
                                                          ttttCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCCCCCGGGGCGCCCCGGgcgc                                          >  1:3794793/1‑101 (MQ=255)
                                                          ttttCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCCCCCCGGACCCCCCGGgggc                                          >  2:807940/1‑101 (MQ=25)
                                                          ttttCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCCATGGACGACGGGGCAGCGCCCCCCCCCGGGGCGCCCCGGgcgc                                          >  1:571366/1‑101 (MQ=255)
                                                            ttCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAg                                        <  2:1791372/101‑1 (MQ=255)
                                                               ttGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGccc                                     <  2:1453932/101‑1 (MQ=255)
                                                               ttGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCCCCCCGGGCGCCCCGGGCCCACccc                                     >  1:2460185/1‑101 (MQ=25)
                                                               ttGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCCCCCCGGGCGCCCCCGGGGCCGccc                                     >  2:4572236/1‑101 (MQ=25)
                                                               tgggggAAAATGCGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGAGGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGccc                                     <  2:2091584/99‑1 (MQ=255)
                                                                tGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGccc.c                                   <  2:5541216/101‑1 (MQ=255)
                                                                 ggggAAAATACGCCGGGGGTCGTCCATTGCGTCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGGCCCCGCCCCGGGTGCGCCGGgggctcacccc                                   >  1:5178535/1‑94 (MQ=25)
                                                                 ggggAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCCCCCCGGGCGGCCCCGGCGCCGGCC.Cg                                  >  2:3988329/1‑101 (MQ=25)
                                                                   ggAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.Cggg                                <  1:1844598/101‑1 (MQ=255)
                                                                     aaaaTACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGc                              <  2:2981126/101‑1 (MQ=255)
                                                                      aaaTACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCCCCCGGGGCGCCCCCGGCGCCGCCC.CCGGGCg                             >  1:1534901/1‑101 (MQ=25)
                                                                        aTACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGaa                           <  1:1187820/101‑1 (MQ=255)
                                                                         tACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGaaa                          <  1:3465012/101‑1 (MQ=255)
                                                                         tACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGaaa                          <  1:2333194/101‑1 (MQ=255)
                                                                          aCGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGAAAt                         <  2:3050016/101‑1 (MQ=255)
                                                                           cGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGAAATc                        <  1:1142414/101‑1 (MQ=255)
                                                                            ccggggggTCGTCCATTGGGGCACCATCGGCTCAAGAC.CCGATGGACGAC.GGGCGGAGCCCCCGGCGGGGGGCCCCCGGGGCCAGCCCGCGGGGcaatacc                        >  1:5503536/4‑95 (MQ=21)
                                                                              cGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGGCGCCCCGGGCGCCGCCC.CGGGGCGgaaaaccc                     >  1:3396695/1‑96 (MQ=25)
                                                                               gggggTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGAAATccccg                    <  1:5560448/101‑1 (MQ=255)
                                                                               gggggTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGGCGCCCCGGGCGCCGCCC.CGGGGGGGAAAccccc                    >  2:3248690/1‑100 (MQ=25)
                                                                                   gtcgtcCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGAAATccccgccca                <  2:2665122/101‑1 (MQ=255)
                                                                                   gtcgtcCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGAAATccccgccca                <  2:2458069/101‑1 (MQ=255)
                                                                                   gtagtcCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGAAATccccgccca                <  2:886014/98‑1 (MQ=255)
                                                                                   gatgtcCTTTGCGCCGCCATCGGTTCAAG.C.TCGATTGACGCCGGGGCATCGCACCCTCCCGGGTAGCCCCGGGCGCAGCCC.CGGGGCGAAATccccgccca                <  1:929945/98‑1 (MQ=25)
                                                                                       tcCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGAAATccccgcccacccc            <  1:4227583/101‑1 (MQ=255)
                                                                                        ccATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGGCGCCCCGGGCGCCGCCC.CGGGGGGAAATccccgcccaccccc           >  2:5236255/1‑101 (MQ=255)
                                                                                         cATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGAAATccccgcccacccccg          >  1:5766627/1‑101 (MQ=255)
                                                                                           ttGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGAAATccccgcccacccccgcc        <  2:5766332/101‑1 (MQ=255)
                                                                                             gcggcgCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCTAAATccccgcccacccccgccca      <  2:1866237/101‑1 (MQ=255)
                                                                                              cggcgCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGAAATccccgcccacccccgcccac     <  2:5007920/101‑1 (MQ=255)
                                                                                              cggcgCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGAAATccccgcccacccccgcccac     <  2:5323662/101‑1 (MQ=255)
                                                                                                gcgcCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGAAATCCccgcccacccccgcccaccc   <  2:1726371/101‑1 (MQ=255)
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CGCGACCTGATGGAAAGACGCGCTGCCGAACCGGCGTGATCCGCCGCGCGCCCCTGCTTTTTCTTGGGGAAAATACGCCGGGGGTCGTCCATTGCGGCGCCATCGGTTCAAG.C.CCGATGGACGACGGGGCAGCGCCCCCGCCCGGGTCGCCCCGGGCGCAGCCC.CGGGGCGAAATCCCCGCCCACCCCCGCCCACCCT  >  NC_014034/2876463‑2876660

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG