Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,925,566 A→C 100% intergenic (+350/‑85) RCAP_rcc02735 → / → RCAP_rcc02736 virulence‑associated protein E/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,925,5660A→C100.0% 1202.8 318intergenic (+350/‑85)RCAP_rcc02735/RCAP_rcc02736virulence‑associated protein E/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (152/166):  ref base (0/0):  total (152/166)

Only 100 of 322 total aligned reads displayed.

AGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAA.TGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGAGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACT  >  NC_014034/2925469‑2925663
                                                                                                  |                                                                                                 
aGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGc                                                                                                 >  2:4675888/1‑101 (MQ=255)
aGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGc                                                                                                 >  2:4843245/1‑101 (MQ=255)
   tGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAtt                                                                                              <  2:1633699/101‑1 (MQ=255)
   tGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAtt                                                                                              <  2:5448830/101‑1 (MQ=255)
      gACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTc                                                                                           <  1:5216083/101‑1 (MQ=255)
       aCAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTcc                                                                                          >  2:1574351/1‑101 (MQ=255)
       aCAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTcc                                                                                          >  1:3580062/1‑101 (MQ=255)
        cAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCt                                                                                         >  2:5736/1‑101 (MQ=255)
         aaGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCtt                                                                                        <  1:5094562/101‑1 (MQ=255)
         aaGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCtt                                                                                        <  2:819211/101‑1 (MQ=255)
          aGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTg                                                                                       <  1:3674506/101‑1 (MQ=255)
            cATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGAt                                                                                     >  1:2437908/1‑101 (MQ=255)
             aTTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATc                                                                                    <  2:5782749/101‑1 (MQ=255)
              ttGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCg                                                                                   >  2:5286942/1‑101 (MQ=255)
              ttGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCg                                                                                   <  1:1589477/101‑1 (MQ=255)
               tGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGa                                                                                  <  2:4120449/101‑1 (MQ=255)
               tGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGa                                                                                  <  1:5670680/101‑1 (MQ=255)
               tGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGa                                                                                  <  1:4075737/101‑1 (MQ=255)
                 ttCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGt                                                                                <  1:1401413/101‑1 (MQ=255)
                  tCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTg                                                                               >  1:1704656/1‑101 (MQ=255)
                  tCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTg                                                                               >  2:4293409/1‑101 (MQ=255)
                   cTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGc                                                                              >  1:2526815/1‑101 (MQ=255)
                    tGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGcc                                                                             >  2:2378816/1‑101 (MQ=255)
                    tGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGcc                                                                             >  1:5652028/1‑101 (MQ=255)
                    tGAAAGGAAAATTTGACGTCCCACTTGAGCCGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGcc                                                                             <  1:2675413/101‑1 (MQ=255)
                     gAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCt                                                                            <  2:5508277/101‑1 (MQ=255)
                     gAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCt                                                                            >  1:1491037/1‑101 (MQ=255)
                      aaaGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTg                                                                           >  1:4162146/1‑101 (MQ=255)
                      aaaGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTg                                                                           >  2:3247068/1‑101 (MQ=255)
                       aaGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGc                                                                          <  1:5338015/101‑1 (MQ=255)
                       aaGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGc                                                                          >  2:2901698/1‑101 (MQ=255)
                        aGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCa                                                                         <  1:783883/101‑1 (MQ=255)
                        aGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCa                                                                         <  1:4361049/101‑1 (MQ=255)
                         ggAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCaa                                                                        >  2:2517840/1‑101 (MQ=255)
                           aaaaTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACg                                                                      >  2:4912225/1‑101 (MQ=255)
                            aaaTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGt                                                                     >  2:2046424/1‑101 (MQ=255)
                             aaTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTg                                                                    <  1:1237421/101‑1 (MQ=255)
                              aTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGa                                                                   <  1:4798323/101‑1 (MQ=255)
                               tttGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGAt                                                                  <  2:4606614/101‑1 (MQ=255)
                                ttGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGAtt                                                                 >  1:393561/1‑101 (MQ=255)
                                ttGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGAtt                                                                 <  1:1004864/101‑1 (MQ=255)
                                  gACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTcc                                                               <  1:443600/101‑1 (MQ=255)
                                  gACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTcc                                                               >  2:5144215/1‑101 (MQ=255)
                                   aCGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTccc                                                              >  2:4051475/1‑101 (MQ=255)
                                    cGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCt                                                             >  2:4473196/1‑101 (MQ=255)
                                    cGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCt                                                             <  2:966358/101‑1 (MQ=255)
                                       cccACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCt                                                          >  1:4650554/1‑101 (MQ=255)
                                        ccACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTc                                                         <  1:3377909/101‑1 (MQ=255)
                                          gatctaGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCAt                                                       <  2:4557176/96‑1 (MQ=255)
                                          aCTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCAt                                                       <  2:774975/101‑1 (MQ=255)
                                          aCTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCAt                                                       >  2:2700651/1‑101 (MQ=255)
                                          aCTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCAt                                                       <  2:4974529/101‑1 (MQ=255)
                                            ttGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGa                                                     <  2:3124790/101‑1 (MQ=255)
                                             tGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGAc                                                    <  2:3435807/101‑1 (MQ=255)
                                              gAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGAcc                                                   <  1:662662/101‑1 (MQ=255)
                                               aGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCAtagatc                                                  >  1:4557452/1‑96 (MQ=255)
                                                 cGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCaaa                                                <  1:2832523/101‑1 (MQ=255)
                                                 cGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCaaa                                                <  2:1171965/101‑1 (MQ=255)
                                                 cGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCaaa                                                <  2:4414631/101‑1 (MQ=255)
                                                   gtgaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGc                                              >  1:4251139/7‑101 (MQ=255)
                                                    tgaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGcc                                             >  1:4724311/6‑101 (MQ=255)
                                                    tgaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGcc                                             >  1:1320272/6‑101 (MQ=255)
                                                     gaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCg                                            >  1:987775/5‑101 (MQ=255)
                                                      aagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGa                                           >  1:216716/4‑101 (MQ=255)
                                                        gtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAg                                         >  2:318326/2‑101 (MQ=255)
                                                            gACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGccc                                     <  1:1844176/101‑1 (MQ=255)
                                                             aCAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCa                                    <  2:1103340/101‑1 (MQ=255)
                                                             aCAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCa                                    <  1:4620411/101‑1 (MQ=255)
                                                               aGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACg                                  <  1:5534432/101‑1 (MQ=255)
                                                               aGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACg                                  <  2:1405142/101‑1 (MQ=255)
                                                                gCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGa                                 >  1:575981/1‑101 (MQ=255)
                                                                   cAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATcc                              <  1:2890825/101‑1 (MQ=255)
                                                                    aaaGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCa                             >  1:3807737/1‑101 (MQ=255)
                                                                     aaGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAg                            <  1:2009566/101‑1 (MQ=255)
                                                                       gCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGt                          <  1:5517934/101‑1 (MQ=255)
                                                                        cGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTg                         <  1:2350592/101‑1 (MQ=255)
                                                                         gAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGa                        <  1:45945/101‑1 (MQ=255)
                                                                         gAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGa                        <  2:3716686/101‑1 (MQ=255)
                                                                             tGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCt                    <  2:1965955/101‑1 (MQ=255)
                                                                             tGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCt                    <  2:2880742/101‑1 (MQ=255)
                                                                               ggACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtc                  >  2:1789470/1‑101 (MQ=255)
                                                                               ggACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtc                  <  2:5769109/101‑1 (MQ=255)
                                                                                 aCATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtcat                <  2:5248735/101‑1 (MQ=255)
                                                                                  cATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtcatc               <  1:402717/101‑1 (MQ=255)
                                                                                    tGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTcatcat             <  1:318359/101‑1 (MQ=255)
                                                                                    tGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTcatcat             >  2:726139/1‑101 (MQ=255)
                                                                                     ggATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATg            <  1:5172795/101‑1 (MQ=255)
                                                                                     ggATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATg            >  2:2576026/1‑101 (MQ=255)
                                                                                        tGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGAcc         <  2:3579845/101‑1 (MQ=255)
                                                                                        tGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGAcc         <  1:2378950/101‑1 (MQ=255)
                                                                                         gCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGAccc        >  2:1195115/1‑101 (MQ=255)
                                                                                          cGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGAcccc       >  2:1452668/1‑101 (MQ=255)
                                                                                           ggTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCg      <  2:4010709/101‑1 (MQ=255)
                                                                                            gTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGa     >  2:5601231/1‑101 (MQ=255)
                                                                                              cGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAAc   <  1:502474/101‑1 (MQ=255)
                                                                                               gggCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACt  >  2:2197796/1‑101 (MQ=255)
                                                                                               gggCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACt  >  2:2310905/1‑101 (MQ=255)
                                                                                               gggCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACt  >  1:2998090/1‑101 (MQ=255)
                                                                                               gggCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACt  >  1:4293064/1‑101 (MQ=255)
                                                                                               gggCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGCCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAAcc  >  1:177756/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
AGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAA.TGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGAGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACT  >  NC_014034/2925469‑2925663

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG