Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,754 T→G 8.7% intergenic (+97/‑63) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7540T→G 8.7% 67.0 ‑0.6 ‑0.1300intergenic (+97/‑63)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (17/9):  ref base (157/117):  total (174/126)
Fisher's exact test strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.96e-01

Only 100 of 326 total aligned reads displayed.

CTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCG  >  NC_014034/2950654‑2950853
                                                                                                     |                                                                                                    
 tGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTg                                                                                                     >  1:2419381/1‑101 (MQ=255)
   aagcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGcc                                                                                                   <  1:2705543/101‑1 (MQ=255)
    agcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCg                                                                                                  <  1:4447134/101‑1 (MQ=255)
      caagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTc                                                                                                <  2:3547016/101‑1 (MQ=255)
       aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                                                               >  1:1575878/1‑101 (MQ=255)
       aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                                                               <  2:651759/101‑1 (MQ=255)
       aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGCCa                                                                                               >  1:5353704/1‑101 (MQ=255)
        agcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAg                                                                                              <  1:3834627/101‑1 (MQ=255)
           cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                                                           >  1:4745375/1‑101 (MQ=255)
           cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                                                           >  1:3595253/1‑101 (MQ=255)
            gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                                                          <  1:3230573/101‑1 (MQ=255)
              gggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAa.g                                                                                       <  1:46081/101‑1 (MQ=255)
               ggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAa.ga                                                                                      >  1:416997/1‑101 (MQ=255)
               ggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAa.ga                                                                                      <  1:3740039/101‑1 (MQ=255)
                 ctctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgg                                                                                    <  2:4536529/101‑1 (MQ=255)
                 ctctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgg                                                                                    <  1:1459071/101‑1 (MQ=255)
                  tctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAggg                                                                                   >  2:2412364/1‑101 (MQ=255)
                   gcttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAA.GAggggg                                                                                 <  2:2217863/91‑1 (MQ=25)
                   gcttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAA.GAggggg                                                                                 <  2:2936325/91‑1 (MQ=25)
                   gcttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAA.GAggggg                                                                                 <  1:5446014/91‑1 (MQ=25)
                    tGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAggggg                                                                                 <  1:1364050/101‑1 (MQ=255)
                     ttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAA.GAggggggc                                                                               >  2:225458/9‑99 (MQ=25)
                     ttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAA.GAggggggc                                                                               >  1:3899732/9‑99 (MQ=25)
                       ccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAg                                                                              <  2:4161964/101‑1 (MQ=255)
                         ccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCt                                                                            <  2:3768320/101‑1 (MQ=255)
                          cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTc                                                                           >  1:1827839/1‑101 (MQ=255)
                          cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTc                                                                           >  1:4185076/1‑101 (MQ=255)
                           ggACCCCCGGGATATTTGAGGCAAGATGAAAAACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGCGAATTTGCCGTCAGGCAAA.GAGGGGGCCGCTcc                                                                          >  2:1621695/1‑101 (MQ=255)
                             aCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTcccc                                                                        >  1:2260465/1‑101 (MQ=255)
                             aCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTcccc                                                                        <  1:1874057/101‑1 (MQ=255)
                               ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCt                                                                      <  2:4649683/101‑1 (MQ=255)
                               ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCt                                                                      >  2:3102007/1‑101 (MQ=255)
                                  cGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGcc                                                                   <  1:1994509/101‑1 (MQ=255)
                                   gggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGccc                                                                  >  2:1468973/1‑101 (MQ=255)
                                    ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGcccc                                                                 >  2:4171926/1‑101 (MQ=255)
                                    ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGcccc                                                                 >  1:5462442/1‑101 (MQ=255)
                                     gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCg                                                                >  2:1121519/1‑101 (MQ=255)
                                     gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCg                                                                <  2:382345/101‑1 (MQ=255)
                                     gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCg                                                                >  2:5216591/1‑101 (MQ=255)
                                       tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGc                                                              >  2:1020118/1‑101 (MQ=255)
                                       tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGc                                                              >  1:4108835/1‑101 (MQ=255)
                                        atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCt                                                             >  1:728439/1‑101 (MQ=255)
                                           tGAGGCAAGATGAACCCCGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGAAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgag                                                          <  1:3186060/101‑1 (MQ=255)
                                             acgCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgaggg                                                        <  1:2838040/99‑1 (MQ=255)
                                              ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                                                       >  2:3022814/1‑101 (MQ=255)
                                                 aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                                                    >  2:504913/1‑101 (MQ=255)
                                                 aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                                                    <  1:3707497/101‑1 (MQ=255)
                                                 aaGATGAAACACGCCCCTTACATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                                                    <  2:558998/101‑1 (MQ=255)
                                                  aGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCg                                                   >  2:2776342/1‑101 (MQ=255)
                                                  aGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCg                                                   >  1:2750955/1‑101 (MQ=255)
                                                  aGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCg                                                   >  1:5304664/1‑101 (MQ=255)
                                                   gATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCgg                                                  >  1:1198652/1‑101 (MQ=255)
                                                     tGAAACACGCCCCTTTCATCGTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                                                <  2:2556331/101‑1 (MQ=255)
                                                        aaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTg                                             >  2:3263268/1‑101 (MQ=255)
                                                          cacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGa                                           >  1:1258849/1‑101 (MQ=255)
                                                          cacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGa                                           >  2:626773/1‑101 (MQ=255)
                                                            cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa                                         <  2:5359931/101‑1 (MQ=255)
                                                             gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                                        >  1:1506227/1‑101 (MQ=255)
                                                             gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                                        <  2:5499502/101‑1 (MQ=255)
                                                                 cTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg                                    <  1:4561799/101‑1 (MQ=255)
                                                                   ttCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCCGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGCGGCGGAACTGGAAACCTTGaa                                  >  2:1365085/1‑101 (MQ=255)
                                                                    tCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                                 <  1:3375346/101‑1 (MQ=255)
                                                                    tCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                                 <  1:2548136/101‑1 (MQ=255)
                                                                     cATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                                >  2:4022111/1‑101 (MQ=255)
                                                                     cATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                                >  1:879099/1‑101 (MQ=255)
                                                                      aTCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAc                               >  2:1264807/1‑101 (MQ=255)
                                                                      aTCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAc                               <  1:691819/101‑1 (MQ=255)
                                                                       tCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCTGCTGAGGGAGGCGGAACTGGGAACCTTGAAGAcc                              >  1:4973419/1‑101 (MQ=255)
                                                                        cTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCTCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                             <  1:5674635/101‑1 (MQ=255)
                                                                        cTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                             <  1:3398722/101‑1 (MQ=255)
                                                                           gCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGcc                          <  2:3845500/101‑1 (MQ=255)
                                                                            ccccAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCt                         <  2:2045754/101‑1 (MQ=255)
                                                                            ccccAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCt                         <  2:1861349/101‑1 (MQ=255)
                                                                            ccccAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCt                         <  1:4487115/101‑1 (MQ=255)
                                                                             cccAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTg                        <  2:4060202/101‑1 (MQ=255)
                                                                             cccAAATATCCC.GGGGGTGAATTTGCCGGCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGAGGAACTGGAAACCTTGAAGACCCGCCTg                        >  2:2888088/1‑101 (MQ=255)
                                                                               cAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGAt                      >  2:4923542/1‑101 (MQ=255)
                                                                                aaaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                     >  2:1535739/1‑101 (MQ=255)
                                                                                aaaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCACCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                     >  2:3356450/1‑101 (MQ=255)
                                                                                 aaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                    >  2:3618767/1‑101 (MQ=255)
                                                                                   tatCCC.GGGGGTGAATTTGCCGTCAGGCAAC.GAGGGGGCAGCTCCCCCTGCCCCGGCTGATGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                  >  1:5142986/1‑101 (MQ=255)
                                                                                   tatCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                  >  2:4481563/1‑101 (MQ=255)
                                                                                   tatCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                  <  1:2298194/101‑1 (MQ=255)
                                                                                      tac.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCCCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGGCCCGCCTGATCGAagag               <  1:1708567/99‑1 (MQ=255)
                                                                                       ccggggggTG.ATTTGCCCGCAGGGAAAGGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAagag               <  1:5012350/98‑1 (MQ=255)
                                                                                        c.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGc             <  1:2988215/101‑1 (MQ=255)
                                                                                          gggggTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc            >  1:5760568/1‑101 (MQ=255)
                                                                                           ggggTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGccc           <  2:229508/101‑1 (MQ=255)
                                                                                            gggTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcg          >  1:4257284/1‑101 (MQ=255)
                                                                                            gggTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcg          <  2:4849777/101‑1 (MQ=255)
                                                                                               tGAATTTGCCGTCAGGCAAA.GAGGGGGGAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAACCCTTGAAGACCCGCCTGATCGAAGAGCCCcgcgc       >  2:5557072/1‑101 (MQ=255)
                                                                                                 aaTTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgcgcgc     >  2:724648/1‑100 (MQ=255)
                                                                                                 aaTTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGa     <  1:643028/101‑1 (MQ=255)
                                                                                                 aaTTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGa     >  1:2332005/1‑101 (MQ=255)
                                                                                                 aaTTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGa     >  1:212318/1‑101 (MQ=255)
                                                                                                   tttGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGGGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc   >  1:2480200/1‑101 (MQ=255)
                                                                                                   tttGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc   >  2:4784453/1‑101 (MQ=255)
                                                                                                   tttGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc   <  2:1558741/101‑1 (MQ=255)
                                                                                                    ttGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCg  >  1:1046224/1‑101 (MQ=255)
                                                                                                    ttGCCGGCAGGCAAAGGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc   <  2:3728353/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
CTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCG  >  NC_014034/2950654‑2950853

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG