Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,044 T→G 7.1% intergenic (+90/+86) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0440T→G 7.1% 50.0 ‑3.2 ‑0.4295intergenic (+90/+86)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (6/15):  ref base (147/127):  total (153/142)
Fisher's exact test strand distribution p-value = 3.94e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.02e-01

Only 100 of 333 total aligned reads displayed.

GCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGC  >  NC_014034/3003944‑3004144
                                                                                                      |                                                                                                    
 ggagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGttt                                                                                                     <  2:5180151/100‑1 (MQ=255)
 cGAGATCTGAGGGCAAGGCTGGGGGCGCTGCCCCCCTCTTGGCCTGACTGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGttt                                                                                                     <  2:824072/101‑1 (MQ=255)
  gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTg                                                                                                    <  1:4038504/101‑1 (MQ=255)
  gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTg                                                                                                    >  1:2855714/1‑101 (MQ=255)
   agaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGc                                                                                                   <  1:2409513/101‑1 (MQ=255)
    gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGcc                                                                                                  >  2:1265430/1‑101 (MQ=255)
    gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGcc                                                                                                  <  1:3977459/101‑1 (MQ=255)
     aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGAAGGCCAATTCACCCCCCGGGCTATTTGGACCAAGGAGAAAGCAG.AT.CCCGGTTTGCCt                                                                                                 >  1:2548418/1‑101 (MQ=255)
       cTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCttt                                                                                               <  2:1637854/101‑1 (MQ=255)
       cTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCCGGGGATTTTGACCAAAGGGGAAGAAG.AA.ACCGGGTTGTCttt                                                                                               >  1:2923037/1‑101 (MQ=21)
         gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCa                                                                                             <  1:536532/101‑1 (MQ=255)
          aGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCAc                                                                                            <  2:74391/101‑1 (MQ=255)
          aGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCAc                                                                                            <  1:3256583/101‑1 (MQ=255)
           gggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCAcc                                                                                           <  1:1219677/101‑1 (MQ=255)
           gggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCAcc                                                                                           <  1:2954194/101‑1 (MQ=255)
              cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTg                                                                                        <  2:2794686/101‑1 (MQ=255)
                aGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGgc                                                                                      >  2:5685855/1‑101 (MQ=255)
                aGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGgc                                                                                      <  2:1934459/101‑1 (MQ=255)
                 ggCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGgcg                                                                                     >  1:4524151/1‑101 (MQ=255)
                    ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCaa                                                                                  >  2:1533493/1‑101 (MQ=255)
                      ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                <  1:4280208/101‑1 (MQ=255)
                      ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                <  1:569480/101‑1 (MQ=255)
                      ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                <  2:1880570/101‑1 (MQ=255)
                       gggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAata                                                                               <  1:4624624/101‑1 (MQ=255)
                       gggCCCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAata                                                                               <  2:1074871/101‑1 (MQ=255)
                         gcgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATc                                                                             <  2:1973950/101‑1 (MQ=255)
                             tGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGGTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                                         >  1:2263295/1‑101 (MQ=255)
                               ccccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggg                                                                       <  2:141762/101‑1 (MQ=255)
                                cccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggggg                                                                      <  2:639434/101‑1 (MQ=255)
                                 ccccTCTGGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggggg                                                                     <  1:1092025/101‑1 (MQ=255)
                                  cccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGa                                                                    <  1:2691568/101‑1 (MQ=255)
                                  cccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGa                                                                    <  2:3004656/101‑1 (MQ=255)
                                   ccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAg                                                                   >  2:1161268/1‑101 (MQ=255)
                                     tctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                                                 >  2:3431356/1‑101 (MQ=255)
                                     tctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                                                 <  1:3821854/101‑1 (MQ=255)
                                      ctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTcc                                                                <  1:1533586/101‑1 (MQ=255)
                                      ctTGGCCTGACGGCCAATTCACCCCCCCGGAACTTTGGACCCAGGCGAAAGCCG.AT.CCCGGCTTGCCTTTTTCCTTGGCGCAAATATCCCggggggcggcc                                                                >  1:203901/1‑96 (MQ=25)
                                       ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTccc                                                               >  1:3206652/1‑101 (MQ=255)
                                        tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                              <  1:3238763/101‑1 (MQ=255)
                                        tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                              >  1:3150515/1‑101 (MQ=255)
                                         ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                             >  2:4306595/1‑101 (MQ=255)
                                          gCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGGTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgcg                                                            >  1:527832/1‑101 (MQ=255)
                                             tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                         >  2:5596744/1‑101 (MQ=255)
                                             tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                         <  1:874968/101‑1 (MQ=255)
                                              gccGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa                                                        <  2:2120826/99‑1 (MQ=255)
                                              gACGGCAAATTCACCCCCCGGGATATTTGGACCAATGTGTAATCAG.AT.CCCGGTTTACCTTTTCCCTTGGCGTAAATATCCCGGGGGGAGTCCCGCGGGGa                                                        <  1:4792017/101‑1 (MQ=21)
                                               aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                       <  2:2189145/101‑1 (MQ=255)
                                                 ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgg                                                     >  2:1745857/1‑101 (MQ=255)
                                                   ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                   >  2:3283456/1‑101 (MQ=255)
                                                   ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                   <  2:5146446/101‑1 (MQ=255)
                                                     aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                 <  2:3354046/101‑1 (MQ=255)
                                                     aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                 >  1:1941944/1‑101 (MQ=255)
                                                     aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                 >  2:5388486/1‑101 (MQ=255)
                                                     aaTTCACCCCCCGGGATATTTGGACCAAGGGGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                 >  1:4137067/1‑101 (MQ=255)
                                                       ttCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                               <  1:1450070/101‑1 (MQ=255)
                                                       ttCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                               <  2:4913549/101‑1 (MQ=255)
                                                       ttCCCCCCCCGGGATATTTGGACCAAGGTGAAAGAAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                               <  1:4964001/101‑1 (MQ=255)
                                                       ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                               >  2:452708/1‑101 (MQ=255)
                                                       ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                               <  2:3717906/101‑1 (MQ=255)
                                                        tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                              <  2:4444519/101‑1 (MQ=255)
                                                          aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                            >  2:4029936/1‑101 (MQ=255)
                                                          aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                            >  2:3852015/1‑101 (MQ=255)
                                                            cccccGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcc                                          >  2:4709125/1‑101 (MQ=255)
                                                               ccGGGATATTTGGCCCAAGGTGAAAGCCG.CG.CCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccc                                       <  1:996060/101‑1 (MQ=17)
                                                               ccGGGATATTTGGCCCAAGGTGAAAGCCG.CG.CCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccc                                       <  1:141484/101‑1 (MQ=17)
                                                                cGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGTTTTGCCTTTCACCTTGGCGAAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                                      <  1:1034182/101‑1 (MQ=255)
                                                                 gggATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                     <  2:2593765/101‑1 (MQ=255)
                                                                 gggATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                     <  2:504233/101‑1 (MQ=255)
                                                                  ggATATTTGGCCCAAGGTGAAAGCCGCGC.CCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                    >  2:2424755/1‑99 (MQ=18)
                                                                  ggATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg                                    >  1:1249964/1‑101 (MQ=255)
                                                                   gATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                   >  1:3761517/1‑101 (MQ=255)
                                                                   gATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                   >  1:5761246/1‑101 (MQ=255)
                                                                    atatTTGGCCCAAGGTGAAAGCCGCGC.CCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                  >  1:1879837/1‑97 (MQ=18)
                                                                    atatTTGGCCCAAGGTGAAAGCCG.CG.CCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                  >  2:3196483/1‑97 (MQ=18)
                                                                    atatTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                                  <  2:4486033/101‑1 (MQ=255)
                                                                    atatTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                                  >  2:313923/1‑101 (MQ=255)
                                                                    atatTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGCGGGGCAGAgcccccctgcc                                  >  1:3414072/1‑101 (MQ=255)
                                                                     tatTTGGCCCAAGGTGAAAGCCG.CG.CCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                 <  1:4207443/101‑6 (MQ=17)
                                                                      atTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                >  1:4982735/1‑95 (MQ=18)
                                                                        ttGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                              >  2:3374518/1‑101 (MQ=255)
                                                                          ggACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                            >  1:509238/1‑101 (MQ=255)
                                                                          ggACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                            >  1:4419420/1‑101 (MQ=255)
                                                                           gACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgg                           <  2:3770732/101‑1 (MQ=255)
                                                                            aCCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                          <  1:773059/101‑1 (MQ=255)
                                                                             ccAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                         >  1:3620013/1‑101 (MQ=255)
                                                                              cAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                        >  1:1631918/1‑101 (MQ=255)
                                                                                   tGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGGGCCCCCCTGCCCCCCTGGGGCCGAgc                   >  1:2345792/1‑101 (MQ=255)
                                                                                   tGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                   >  2:5072471/1‑101 (MQ=255)
                                                                                    gAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg                  >  1:5532143/1‑101 (MQ=255)
                                                                                      aaGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt                >  2:4198156/1‑101 (MQ=255)
                                                                                       aGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTc               >  1:5049249/1‑101 (MQ=255)
                                                                                          cg.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg            <  2:4666875/100‑1 (MQ=255)
                                                                                             aT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCg          >  2:3596632/1‑101 (MQ=255)
                                                                                             aT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCg          >  2:2865844/1‑101 (MQ=255)
                                                                                                 ccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtgt       <  1:2155523/101‑1 (MQ=255)
                                                                                                 ccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtgt       <  2:1087887/101‑1 (MQ=255)
                                                                                                   ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCCGCGCTCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt     >  2:2295883/1‑101 (MQ=255)
                                                                                                   ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt     <  1:4459229/101‑1 (MQ=255)
                                                                                                    gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg    >  2:5546188/1‑101 (MQ=255)
                                                                                                      ttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGc  >  1:515932/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
GCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.AT.CCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGC  >  NC_014034/3003944‑3004144

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG