Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,178,410 T→C 3.1% intergenic (+149/+98) RCAP_rcc02987 → / ← RCAP_rcc02988 OmpA/MotB domain‑containing protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,178,4100T→C 3.1% 3.6 ‑2.1 ‑0.0189intergenic (+149/+98)RCAP_rcc02987/RCAP_rcc02988OmpA/MotB domain‑containing protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/3):  ref base (38/144):  total (41/148)
Fisher's exact test strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.42e-01

Only 100 of 230 total aligned reads displayed.

CGCGCGGCATTGGCCCCATGCCAGCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCTTTTCGCCCGAAACCGTCGCTC  >  NC_014034/3178313‑3178509
                                                                                                  |                                                                                                      
cgcgcgGCATTGGCCCCATGCCAGCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCgg                                                                                                     <  1:2370314/101‑1 (MQ=255)
   ggggCTTTGGCCCCGGGCCGGCCGGGGGCCCAAGGGCCGCCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCt                                                                                                  <  1:1208742/99‑1 (MQ=17)
   gcgGCATTGGCCCCATGCCAGCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCt                                                                                                  <  1:3078270/101‑1 (MQ=255)
   gcgGCATTGGCCCCATGCCAGCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCt                                                                                                  <  1:391047/101‑1 (MQ=255)
   gcgGCATTGGCCCCATGCCAGCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCt                                                                                                  <  1:5635601/101‑1 (MQ=255)
   gcgGCATTGCCCCCATGCCAGCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCt                                                                                                  <  2:110364/101‑1 (MQ=255)
      gCACTGGCCCCATGCCCGCCGGGGGCCCAAAGGCCGCCGGCCAGCCCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCtgtg                                                                                               <  2:1750762/101‑1 (MQ=255)
       cATGGGCCCCATGCCAGCCGGGGGCCCAAAGGCCCCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTgtgt                                                                                              <  1:867812/101‑1 (MQ=255)
        aTTGGCCCCATGCCAGCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTc                                                                                             <  1:5657321/101‑1 (MQ=255)
        aTTGGCCCCATGCCAGCCGGGGCCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTc                                                                                             <  2:965421/101‑1 (MQ=255)
        aTTGGCCCCATCCCAGCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTc                                                                                             <  2:5503247/101‑1 (MQ=255)
        aTTGCCCCCATGCCAGCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTc                                                                                             <  1:2877713/101‑1 (MQ=255)
          tgcccacggcccAGCGGGGGGCCGAAGGGCCGCCGGCAGGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTcgc                                                                                           <  2:2451573/91‑1 (MQ=14)
              ccctgcccAGCCGGGGGCCCAAGGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCtt                                                                                       <  2:4686520/95‑1 (MQ=255)
              cccATGCCAGCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCtt                                                                                       <  2:3368475/101‑1 (MQ=255)
               ccATGCCAGCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTg                                                                                      <  1:165509/101‑1 (MQ=255)
                cagcgcatccGGGGCGCAAAAGGCCCCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTgg                                                                                     <  1:63915/93‑1 (MQ=11)
                 aTGCCAGCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.CTCTCCCCCGCCCGGCCCGGCGGCGGCCCGGGGCgcgccttcg                                                                                    >  2:2518086/1‑96 (MQ=14)
                  gcacAGCGGGGCCCCCGAAGCCCGCGGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCTGGCGTCGGCCTGTGTCGCGCTTgggg                                                                                   <  2:3654395/98‑1 (MQ=17)
                   gCCAGCGGGGGGCCCAAGGGCCCCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGa                                                                                  <  1:1127960/101‑1 (MQ=255)
                     cAGCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.CTCTCGCCCCCCGGGGCGGGCGGCGGCCCGGGGCGCGCGTGGGGgaa                                                                                >  1:398828/1‑101 (MQ=14)
                      aGCGGGGGGCCGAAAGGCCGCCGGCCAGCCCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAAc                                                                               <  2:2473098/101‑1 (MQ=255)
                      aGCGGGGGGCCCAAAGGCCGCCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAAc                                                                               <  2:2911271/101‑1 (MQ=255)
                       gCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACg                                                                              <  1:472581/101‑1 (MQ=255)
                          gggggCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTc                                                                           <  2:3667984/101‑1 (MQ=255)
                           ggggCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGGGC.GTCTCGCCCCCCCGGCCCGGGGGCGGCCCGTGTGGCGCGTCGGGGGACGGGCt                                                                          >  2:2730264/1‑101 (MQ=2)
                           gggcCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCt                                                                          <  2:3074044/101‑1 (MQ=255)
                            gggCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGGGC.GTCTCGCCCCCCCGGCCGGGCGCCGGGCCGCGGCGCGGTGgggggggcggcgtt                                                                         >  1:2641525/1‑91 (MQ=2)
                             ggCCCAAGGGCCCCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTg                                                                        <  1:771416/101‑1 (MQ=255)
                             ggCCCAAGGCCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTg                                                                        <  2:2092669/101‑1 (MQ=255)
                             ggCCCAAAGGCCCCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCCCCGGGCCGGGCGGCGGGCCCTGTCACCCTTGGGGGACCgggcgcg                                                                        >  2:1615476/1‑96 (MQ=11)
                              gCCCAAGGGCCGCCGCCCAGCCCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGc                                                                       <  2:1051421/101‑1 (MQ=255)
                               cccgagggCCGCGGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGACGGGCGCTGGCCTCTGTTGTGCTTGGGGAGGCGGTCTTGcg                                                                      <  1:394273/95‑2 (MQ=17)
                               cccCAAGGCCCCCGGCCCGGCCCCGGCCCCCCCCTGGGGGGGCG.TTCTCCCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGcc                                                                      <  1:4478556/101‑1 (MQ=12)
                               cccAAGGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGcc                                                                      <  1:2459820/101‑1 (MQ=255)
                                      gcccccGGCCGCCGCCCGCCCCGCCCGTGGCGGGCGC.TTCTCGCCCGCCGGGACGGGCGCCGCCCTCTGTCGCGCTTGGGAAAACGGTCTTgtcgcaaccg                                                               <  2:905999/101‑10 (MQ=17)
                                       cgtgcgcccggcgcCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCt                                                              <  2:4878715/91‑1 (MQ=255)
                                       ccgccgGCCAGCGCCCGCCCCGCCCATGGCGGGGGC.GTCTCCCCCCCCCGGCCGGGCGGCGGCCTGTGTGGCGCTTGGGGGGAAGGGCTTGCCCCCGGccc                                                              >  2:215830/1‑100 (MQ=9)
                                        cccgggCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCtt                                                             <  1:2157505/97‑1 (MQ=255)
                                           cgGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGca                                                          <  1:2575443/101‑1 (MQ=255)
                                           cgGCCAGCCCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGca                                                          <  1:2520464/101‑1 (MQ=255)
                                             gCCGGCCCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGcaca                                                        <  1:2010362/101‑1 (MQ=255)
                                             gCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGcaca                                                        <  1:3163281/101‑1 (MQ=255)
                                                 gcgcCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGGGGGGCGCAGGCCTCCCCCGCGCTTGGGGGGGGGATCTTGTCACCGGCCTTgggcggcc                                                     >  1:1511485/1‑94 (MQ=18)
                                                 gcgcCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAggcg                                                    <  2:3164891/101‑1 (MQ=255)
                                                   gcccgcccCGCCCATGGCGGGGGG.TTCTTTCCCCCCCGGCCCGGGGGGGGCCCCGGTCGCGCCTGGGGGGACGGGCTTGGCACGGGGCCCGCCCAcgcggg                                                  >  2:1721113/1‑101 (MQ=2)
                                                   gcccgcccCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGGCGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTTggtgcggcgg                                                   >  2:1819880/1‑92 (MQ=18)
                                                   gcccgcccCGCCCATGGCGGGCGCATTCGCGCCGCCCGGGGGGGGCGCAGGCCTCCCGCGCGCTTGGGGAGGGGATCTTGTCACCGGCTTTggtgcggccg                                                   >  2:2378991/1‑92 (MQ=18)
                                                     ccgccccgccCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAggcgggcg                                                <  1:2849015/101‑1 (MQ=255)
                                                         cccgcccATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGaa                                            <  1:4134280/101‑1 (MQ=255)
                                                         cccgcccATGGCGGGCGC.TTCTCGCCCGCCGGACCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGaa                                            <  2:4540784/101‑1 (MQ=255)
                                                          ccgcccATGGCGGGGGC.TTCTCGCCCGCCCGGCCGGGGGGCGGCCTGTGTCGCGCTTGGGGGAACGGGGTTGCCACCGGCCCTGGACAGGcgggggggggg                                           >  2:4597618/1‑98 (MQ=255)
                                                          ccgcccATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGaaa                                           <  1:930647/101‑1 (MQ=255)
                                                           cgcccATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAAc                                          <  2:4042254/101‑1 (MQ=255)
                                                           cgcccATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAAc                                          <  1:5307620/101‑1 (MQ=255)
                                                             cccATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAAcgc                                        <  2:4745805/101‑1 (MQ=255)
                                                               cATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCgg                                      <  1:3675664/101‑1 (MQ=255)
                                                               cATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCgg                                      <  1:3408067/101‑1 (MQ=255)
                                                                 tGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGAt                                    <  1:355129/101‑1 (MQ=255)
                                                                  ggcgggcgC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATc                                   <  1:1398615/101‑1 (MQ=255)
                                                                   gcgggcgC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATc.g                                 <  2:4384930/101‑1 (MQ=255)
                                                                   gcgggcgC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATc.g                                 <  1:4778061/101‑1 (MQ=255)
                                                                    cgggcgC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGGGGGgaaaacggggcgc                                 >  2:3751364/1‑91 (MQ=21)
                                                                       gcgc.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGGAAAGCgggcaccggt                              >  2:612814/1‑93 (MQ=21)
                                                                        cgc.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.Gttt                          <  1:3833037/101‑1 (MQ=255)
                                                                         gc.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGGGGGGAAAGCGGCGC.G.C.GGTTc                         >  2:5191611/1‑101 (MQ=25)
                                                                         gc.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGGAAAGCCGATC.G.C.GGTTc                         >  2:1517195/1‑101 (MQ=25)
                                                                          c.TTCTCGCCCGGCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTcc                        <  2:2615400/101‑1 (MQ=255)
                                                                            ttCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGGGGGGAAACCGGGTC.G.G.GCTTCCt                       >  1:4649991/1‑101 (MQ=25)
                                                                            ttCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCt                       <  1:3317679/101‑1 (MQ=255)
                                                                            ttCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCt                       <  2:29760/101‑1 (MQ=255)
                                                                             tctcGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGGGGGGAAAGCGGGTC.G.C.Gttttcct                      >  2:5746366/1‑97 (MQ=25)
                                                                             tctcGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCtt                      <  2:4313947/101‑1 (MQ=255)
                                                                             tctcGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCtt                      <  2:1726859/101‑1 (MQ=255)
                                                                              ctcGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGGGGGGAAAGCGGGTC.G.C.GGTTTCttt                     >  2:5648596/1‑101 (MQ=37)
                                                                                cgcccgccGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGGGGGGAAAGGGGGTC.G.G.GGTTTCttttt                   >  1:1676291/1‑100 (MQ=21)
                                                                                 gcccgccGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGGGGGGGAAGCGGGGCGG.C.GTTTCTTTTTc                   >  1:1684443/1‑101 (MQ=25)
                                                                                 gcccgccGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCTTTTCg                  <  2:1685969/101‑1 (MQ=255)
                                                                                  cccgccGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCTTTTCGc                 <  2:5319087/101‑1 (MQ=255)
                                                                                   ccgccgGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGGGGGGAAAGCGGATC.G.C.GGTTCCttttttcc                >  1:535187/1‑97 (MQ=37)
                                                                                    cgccgGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGGGGGGAACGCGGAGT.G.G.GGTTTCTTTTTGccc               >  2:5286150/1‑101 (MQ=37)
                                                                                    cgccgGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCTTTTCGccc               <  1:2332229/101‑1 (MQ=255)
                                                                                    cgccgGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCTTTTCGccc               <  1:996202/101‑1 (MQ=255)
                                                                                    cgccgGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCTTTTCGccc               <  1:3721165/101‑1 (MQ=255)
                                                                                      ccgggccgggCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGGGGGGAACGGGGGGC.G.G.GGTTTCttttttccccc             >  1:2098379/1‑94 (MQ=25)
                                                                                      ccgggccgggCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCTTTTCGCCCGa             <  2:3272533/101‑1 (MQ=255)
                                                                                       cgggccgggcGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGGGAAGCGGGAC.G.CGGTTTCCtttttcccgca             >  2:5032116/1‑94 (MQ=25)
                                                                                        gggccgggcGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGGAACGCGGGTC.G.C.GGTTCCTTTTTGccccgaa           >  2:769422/1‑97 (MQ=38)
                                                                                        gggccgggcGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCTTTTCGCCCGaaa           <  2:3909617/101‑1 (MQ=255)
                                                                                        gggccgggcGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCTTTTCGCCCGaaa           <  2:938460/101‑1 (MQ=255)
                                                                                          gccgggcGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCTTTTCGCCCGAAAcc         <  2:3421305/101‑1 (MQ=255)
                                                                                           ccgggcGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGGCGGGAACGCCGAGG.G.C.GGGTTCTTTTAGCCCGAAAcaa        >  2:4147801/1‑99 (MQ=25)
                                                                                            cgggcGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGGAACGCGGGAC.G.C.GGTTTCTTTTTTCCCCGAACCgg       >  2:4061141/1‑100 (MQ=25)
                                                                                             gggcGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCTTTTCGCCCGAAACCGTc      <  2:5346825/101‑1 (MQ=255)
                                                                                              ggcGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCTTTTCGCCCGAAACCGTCg     <  2:1401412/101‑1 (MQ=255)
                                                                                               gcGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCTTTTCGCCCGAAACCGTCGc    <  2:5269157/101‑1 (MQ=255)
                                                                                                cGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGGAAAGCGGATC.G.C.GTTTTCTTTTCTCCCCAAACCCTCGcc   >  1:572179/1‑100 (MQ=37)
                                                                                                cGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCTTTTCGCCCGAAACCGTCGCt   <  2:4648943/101‑1 (MQ=255)
                                                                                                 gTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGGAAAGCGGGGC.GCC.GTTTCCTTTTGCCCCAAAAccccgctc   >  2:72595/1‑95 (MQ=25)
                                                                                                  tCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAGAAGCGGGGC.G.CGGTTTCTTTTTCCCCCCAAAccccccccc  >  1:3066902/1‑94 (MQ=25)
                                                                                                  |                                                                                                      
CGCGCGGCATTGGCCCCATGCCAGCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGC.TTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGTCTTGCCACGGGCCTTGCACAGGCGGGCGGGAAACGCGGATC.G.C.GTTTCCTTTTCGCCCGAAACCGTCGCTC  >  NC_014034/3178313‑3178509

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG