Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,333,494	C→G	100%	intergenic (+40/‑126)	ccmH → / → paaG	cytochrome c‑type biogenesis protein CcmH/enoyl‑CoA hydratase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,333,494	0	C→G	100.0%	105.2	28	intergenic (+40/‑126)	ccmH/paaG	cytochrome c‑type biogenesis protein CcmH/enoyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  new base (1/27):  ref base (0/0):  total (1/27)

GTGCCGGTGCTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGCGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCCGCGCG  >  NC_014034/3333398‑3333591                                                                                                 |                                                                                                  gtgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCt                                                                                               <  2:3281879/101‑1 (MQ=255)     cggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGc                                                                                           <  2:4724758/101‑1 (MQ=255)      ggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcc                                                                                          >  2:5314355/1‑101 (MQ=255)        tgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccc                                                                                        <  1:1223887/101‑1 (MQ=255)         gcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGccccc                                                                                       <  1:2773407/101‑1 (MQ=255)         gcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGccccc                                                                                       <  2:1624983/101‑1 (MQ=255)         gcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGccccc                                                                                       <  2:850625/101‑1 (MQ=255)           tctcGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCg                                                                                     <  1:2740298/101‑1 (MQ=255)           tctcGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCg                                                                                     <  2:1764507/101‑1 (MQ=255)           tctcGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCg                                                                                     <  2:151640/101‑1 (MQ=255)           tcgcGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCg                                                                                     <  2:750472/98‑1 (MQ=255)            ctcccgatctgAAGAGGCGCGGCTGAAAGCCCTGCCGAAGGACTAGGGATTTCGCCCGGGCCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGc                                                                                    <  2:1515804/91‑1 (MQ=25)            ctcGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGc                                                                                    <  1:4815734/101‑1 (MQ=255)            ctcGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGc                                                                                    <  1:1797243/101‑1 (MQ=255)              cGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAc                                                                                  <  1:5314645/101‑1 (MQ=255)              cGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAc                                                                                  <  1:4990589/101‑1 (MQ=255)              cGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAc                                                                                  <  2:2120104/101‑1 (MQ=255)                gCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAccc                                                                                <  2:478175/101‑1 (MQ=255)                 ccGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAcccc                                                                               <  2:4060504/101‑1 (MQ=255)                   gAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAcccccc                                                                             <  2:926869/101‑1 (MQ=255)                   gAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAcccccc                                                                             <  2:2208972/101‑1 (MQ=255)                      gAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGCGGGCGCCCCCCCCCGccggccggtag                                                                          >  1:1515897/1‑91 (MQ=255)                       aaGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGc                                                                         <  2:938659/101‑1 (MQ=255)                                ggCTGAAAGCCCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGc                                                                <  2:3932518/101‑1 (MQ=255)                                ggCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCGGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGc                                                                <  2:5047660/101‑1 (MQ=255)                                        gACCTGCTGAAGGACTAGGGATTTCGCCGGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGaaa                                                        <  2:5457074/101‑1 (MQ=255)                                                           gATTTCGCCCGGGCCTTGCGCCCCGGGCCACGCGCGGGGGGCTCCGCCCCCTGAAACCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTATGGTCGTGa                                     <  1:900243/101‑1 (MQ=21)                                                                cGCCCGGGCCTTGCGCCCCGGGCGCCCCGCGGGGGCCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGttt                                <  2:3695130/101‑1 (MQ=25)                                                                            gggcCCCGGGGGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTg                    <  1:1582678/99‑1 (MQ=255)                                                                            gcgcCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTg                    <  2:3890041/101‑1 (MQ=255)                                                                                       gACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGgcgc         <  1:5219032/101‑1 (MQ=255)                                                                                              ggggggCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGGCTGGGCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCcgcgcg  >  2:4447384/1‑101 (MQ=255)                                                                                                 |                                                                                                  GTGCCGGTGCTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGCGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCCGCGCG  >  NC_014034/3333398‑3333591 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 37 ≤  ATCG