Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,398,929 C→T 100% E212K (GAG→AAG)  tolQ ← TolQ protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,398,9290C→T100.0% 909.7 231E212K (GAG→AAG) tolQTolQ protein
Reads supporting (aligned to +/- strand):  new base (122/109):  ref base (0/0):  total (122/109)

Only 100 of 276 total aligned reads displayed.

CTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCGGC  >  NC_014034/3398831‑3399026
                                                                                                  |                                                                                                 
 tttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtc                                                                                                <  2:1623816/101‑1 (MQ=255)
  ttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtcg                                                                                               <  2:2378335/101‑1 (MQ=255)
     tcatcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcg                                                                                            <  2:4091071/101‑1 (MQ=255)
     tcatcaCCCCCGCGCCCATCGCGTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcg                                                                                            <  2:2301090/101‑1 (MQ=255)
      catcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgt                                                                                           >  1:2671729/1‑101 (MQ=255)
      cagcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgt                                                                                           <  2:1853751/98‑1 (MQ=255)
       atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                                                                          <  2:3540649/101‑1 (MQ=255)
        tcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcg                                                                                         >  1:710464/1‑101 (MQ=255)
         caCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcgg                                                                                        <  1:802780/101‑1 (MQ=255)
         caCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcgg                                                                                        <  1:2532406/101‑1 (MQ=255)
           cccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGAc                                                                                      >  2:4205562/1‑101 (MQ=255)
           cccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGAc                                                                                      >  1:5525441/1‑101 (MQ=255)
            ccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACg                                                                                     >  1:2763403/1‑101 (MQ=255)
             cccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACga                                                                                    <  2:4674179/101‑1 (MQ=255)
               cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgagg                                                                                  >  2:3850383/1‑100 (MQ=255)
               cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                                                                  <  2:4403508/101‑1 (MQ=255)
               cgcgCCCATCGCTTACGCGGCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                                                                  <  1:4085222/101‑1 (MQ=255)
                gcgcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGACGCCCGAGGTGATCTTGTCGGCCTCGGACGagag                                                                                 >  1:4964457/1‑101 (MQ=255)
                 cgcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGc                                                                                >  1:4365720/1‑101 (MQ=255)
                  gcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCt                                                                               <  1:2036386/101‑1 (MQ=255)
                    ccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttg                                                                             >  2:4503774/1‑101 (MQ=255)
                    ccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttg                                                                             >  2:2713760/1‑101 (MQ=255)
                     cATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgt                                                                            >  1:5489959/1‑101 (MQ=255)
                     cATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgt                                                                            >  1:2829817/1‑101 (MQ=255)
                     cATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgt                                                                            <  1:4893929/101‑1 (MQ=255)
                      aTCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgtt                                                                           <  1:3705206/101‑1 (MQ=255)
                      aTCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgtt                                                                           >  1:3463562/1‑101 (MQ=255)
                       tCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgttg                                                                          >  1:4781389/1‑101 (MQ=255)
                        cGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTtgttgt                                                                         <  2:4311502/101‑1 (MQ=255)
                        cGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTtgttgt                                                                         <  1:2457255/101‑1 (MQ=255)
                         gCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTa                                                                        <  2:4031092/101‑1 (MQ=255)
                         gCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTa                                                                        <  1:311726/101‑1 (MQ=255)
                         gCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTa                                                                        <  2:5489669/101‑1 (MQ=255)
                          cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                                                                       >  1:98476/1‑101 (MQ=255)
                          cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                                                                       <  2:2985404/101‑1 (MQ=255)
                           ttACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAgg                                                                      >  1:1122682/1‑101 (MQ=255)
                            tACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGGGAGCTTGTTGTAGGc                                                                     >  2:2814874/1‑101 (MQ=255)
                            tACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGc                                                                     >  1:4392623/1‑101 (MQ=255)
                            tACGCGCCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGc                                                                     <  1:838423/101‑1 (MQ=255)
                             aCGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCg                                                                    <  1:473851/101‑1 (MQ=255)
                              cgcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGa                                                                   >  2:5229412/1‑101 (MQ=255)
                               gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                                                                  <  2:5642455/101‑1 (MQ=255)
                               gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                                                                  <  1:1337319/101‑1 (MQ=255)
                                  tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc                                                               <  1:5402211/101‑1 (MQ=255)
                                  tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATCAc                                                               <  1:3300022/101‑1 (MQ=255)
                                   ccAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAcc                                                              >  1:4159587/1‑101 (MQ=255)
                                      gCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgcc                                                           >  2:764577/1‑101 (MQ=255)
                                       cTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg                                                          >  1:1182320/1‑101 (MQ=255)
                                        tGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCgg                                                         >  2:4574858/1‑101 (MQ=255)
                                           cgcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGAt                                                      <  2:627550/101‑1 (MQ=255)
                                               acaggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgcg                                                  >  2:1103680/4‑101 (MQ=255)
                                               acaggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgcg                                                  <  1:1940328/98‑1 (MQ=255)
                                               acaggATGGTCGAGAATTCGTCGGCGAAGGCCTCGCACCCCGAGGCGATCTTCTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgcg                                                  >  1:3225339/4‑101 (MQ=255)
                                                caggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcgg                                                 >  2:3326397/3‑101 (MQ=255)
                                                 aggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcggc                                                <  2:5367874/100‑1 (MQ=255)
                                                 aggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcggc                                                <  2:3710321/100‑1 (MQ=255)
                                                 aggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcggc                                                <  1:57990/100‑1 (MQ=255)
                                                  ggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcggcg                                               >  2:98417/1‑101 (MQ=255)
                                                     tGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACa                                            <  1:3362183/101‑1 (MQ=255)
                                                      ggTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACaa                                           <  2:35635/101‑1 (MQ=255)
                                                       gTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAg                                          <  1:5217554/101‑1 (MQ=255)
                                                         cGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGc                                        >  2:643356/1‑101 (MQ=255)
                                                         cGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGc                                        >  2:5722288/1‑101 (MQ=255)
                                                         cGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGc                                        <  2:4918231/101‑1 (MQ=255)
                                                          gagaATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGcc                                       <  1:4606971/101‑1 (MQ=255)
                                                           agaATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGcag                                      >  2:30480/1‑99 (MQ=255)
                                                            gaATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGa                                     >  1:5142964/1‑101 (MQ=255)
                                                            gaATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGa                                     >  2:4773275/1‑101 (MQ=255)
                                                             aaTTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGAt                                    >  1:1751145/1‑101 (MQ=255)
                                                               ttCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCg                                  <  1:3515130/101‑1 (MQ=255)
                                                                tcgtcgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGc                                 <  2:4636881/101‑1 (MQ=255)
                                                                     ggCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTgg                            >  2:1205201/1‑101 (MQ=255)
                                                                     ggCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTgc                            >  2:1488935/1‑100 (MQ=255)
                                                                       cGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGcc                          >  2:2215655/1‑101 (MQ=255)
                                                                        gAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGcca                         >  1:4513510/1‑101 (MQ=255)
                                                                        gAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGcca                         >  1:4677302/1‑101 (MQ=255)
                                                                         aaGGCCTCGGAGCCCGAGGTTATCATGTCGGCTTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccac                        <  2:1972035/101‑1 (MQ=255)
                                                                          aGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGCGGccacc                       >  2:3147432/1‑101 (MQ=255)
                                                                             ccTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAgc                    >  2:366636/1‑101 (MQ=255)
                                                                             ccTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAgc                    <  1:2230051/101‑1 (MQ=255)
                                                                              cTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAgcg                   >  1:2962580/1‑101 (MQ=255)
                                                                                cGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGcc                 <  2:1168693/101‑1 (MQ=255)
                                                                                cGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGcc                 >  2:2327771/1‑101 (MQ=255)
                                                                                cGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGcc                 >  2:2942898/1‑101 (MQ=255)
                                                                                 gTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCt                >  1:3749939/1‑101 (MQ=255)
                                                                                 gTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCt                <  1:680118/101‑1 (MQ=255)
                                                                                   aGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCg              >  1:1228922/1‑101 (MQ=255)
                                                                                   aGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCg              <  1:2327611/101‑1 (MQ=255)
                                                                                     cccGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGga            >  2:1678165/1‑101 (MQ=255)
                                                                                       cGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGgaga          <  1:5706483/101‑1 (MQ=255)
                                                                                       cGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGgaga          >  2:2657707/1‑101 (MQ=255)
                                                                                       cGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGgaga          >  2:3299318/1‑101 (MQ=255)
                                                                                       cGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGgaga          >  2:4149276/1‑101 (MQ=255)
                                                                                         gggTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATc        <  2:1269871/100‑1 (MQ=255)
                                                                                         gggTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATc        <  2:5025720/100‑1 (MQ=255)
                                                                                         aGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATc        >  1:1686921/1‑101 (MQ=255)
                                                                                             gATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCg    >  2:3600255/1‑101 (MQ=255)
                                                                                             gATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCg    >  1:5301159/1‑101 (MQ=255)
                                                                                             gATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCg    >  1:3920071/1‑101 (MQ=255)
                                                                                              aTCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCgg   >  1:4279981/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCGGC  >  NC_014034/3398831‑3399026

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG