Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_014034 | 3,439,799 | G→C | 3.6% | intergenic (‑102/+61) | RCAP_rcc03240 ← / ← mutB | ATPase AAA/methylmalonyl‑CoA mutase large subunit |
Read alignment evidence... | ||||||||||
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seq id | position | change | freq | score | reads | annotation | genes | product | ||
* | NC_014034 | 3,439,799 | 0 | G→C | 3.6% | 2.4 ‑0.4 ‑0.0 | 138 | intergenic (‑102/+61) | RCAP_rcc03240/mutB | ATPase AAA/methylmalonyl‑CoA mutase large subunit |
Reads supporting (aligned to +/- strand): new base (3/2): ref base (61/71): total (65/73) | ||||||||||
Fisher's exact test strand distribution p-value = 6.64e-01 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.65e-01 |
Only 100 of 212 total aligned reads displayed.
GCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATC > NC_014034/3439699‑3439901
|
gcggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGgctttg < 1:147042/101‑1 (MQ=255)
cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGgctttgc.ttt < 1:1196832/101‑1 (MQ=255)
cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGgctttgc.ttt < 1:5172979/101‑1 (MQ=255)
cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGgctttgc.ttt < 2:4663039/101‑1 (MQ=255)
cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGgctttgc.ttt < 1:312679/101‑1 (MQ=255)
cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAGAGTTGGGGCGAG.AGT.AAAGGgg.gttgc.ttt > 2:4113304/1‑101 (MQ=255)
gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.tttt < 2:1046667/101‑1 (MQ=255)
aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGCAG.A.G.AAAAGGGGTTTGG.ttttt > 1:1005856/1‑100 (MQ=255)
aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTa < 1:4188290/101‑1 (MQ=255)
ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATc < 1:2284241/101‑1 (MQ=255)
tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGAGATTTCGGGGAAA.G.T.AAAAGGGCTTTTG.ttttttttt > 2:605521/1‑96 (MQ=255)
acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTCGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTcc < 1:4266590/101‑1 (MQ=255)
cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTTGGGAAG.A.C.AAAGGGGGGTTGT.TTTTTTTTTTtcc > 1:3398320/1‑101 (MQ=255)
cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGAAGAA.A.AAAGGGGTTTTGT.TTTTTTTTTTcc > 1:2545048/1‑101 (MQ=255)
cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTccc < 2:2084486/101‑1 (MQ=255)
gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCa < 2:2249901/101‑1 (MQ=255)
cgcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCaa < 2:4539411/101‑1 (MQ=255)
cgcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCaa < 2:1473860/101‑1 (MQ=255)
cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAat < 1:1099154/101‑1 (MQ=255)
cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAat < 2:670569/101‑1 (MQ=255)
cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAat < 1:2449382/101‑1 (MQ=255)
gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGCAG.A.G.AAAAGGGGGTTGG.TTTTTTTTTTCCCCaaaaa > 1:5719132/1‑99 (MQ=255)
gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGGAAG.A.T.AAAAGGGGTTTTC.TTTTTTTTTTTCCCCAata > 1:2234420/1‑101 (MQ=37)
gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAata < 2:2639923/101‑1 (MQ=255)
aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAG.A.T.AAAAGGGGTTTGC.TTTTTTTTTTTCCCAAatat > 2:3319628/1‑101 (MQ=255)
ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATc < 2:3159509/101‑1 (MQ=255)
ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATc < 1:55675/101‑1 (MQ=255)
ccAGCTTTCAAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATc < 1:1279130/101‑1 (MQ=255)
cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGGAG.A.A.AAAAGGGGTTTGCTTTTTTTTTTTCCCCaaaattc > 1:4289374/1‑97 (MQ=255)
cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATcc < 2:4434519/101‑1 (MQ=255)
aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATccc < 2:4877002/101‑1 (MQ=255)
aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATccc < 1:4507855/101‑1 (MQ=255)
aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATccc < 2:124460/101‑1 (MQ=255)
gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGCTTTTGGGGCAAG.A.TAAAAGGGGTTTTCC.TTTTTTTTTTTCCCaatttcccg > 1:3573256/1‑94 (MQ=255)
cTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCgg < 2:4921705/101‑1 (MQ=255)
cTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCgg < 2:2552255/101‑1 (MQ=255)
cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGCAAG.A.T.AAAAGGGCTTTGC.TTTTTTCTTTTCCCCAATATCCCgggggg > 2:4414719/1‑100 (MQ=37)
cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..t < 2:2413711/101‑1 (MQ=255)
cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..t < 2:2338883/101‑1 (MQ=255)
cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..t < 1:5525741/101‑1 (MQ=255)
gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..Tg < 1:2642433/101‑1 (MQ=255)
aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGc < 2:5637183/101‑1 (MQ=255)
aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGCGAG.A.A.AAAAGGGCTTTGCTTTTTTTTTTTCCCCAAAATTCCCgggggggg > 1:381717/1‑98 (MQ=255)
aGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGAAG.A.T.AAAGGGGGGTTGT.TTTTTTTTTTTCCCCAATATCCCcggggggggg > 2:509282/1‑96 (MQ=255)
gACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..ggg < 1:5763491/101‑1 (MQ=255)
aCAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAG.A.T.AAAGGGGGTTTGG.TTTTTTTTTTTCCCACATATACCCGGGG..GGC..gggg > 1:5153494/1‑101 (MQ=255)
aCAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATAATTGGGGCAAG.A.T.AAAGGGGCCTTGC.TTTTATCTTTCTCCAAACATCCCCGGGG..GGC..ggcg > 2:5744536/1‑99 (MQ=25)
aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGAATTTGGGGGAAG.A.T.AAAGGGGGTTTGC.TTTTTTCTTTCCCCAAATATCCCGGGGG..GGC..GGGGGc > 1:5432536/1‑101 (MQ=38)
aGGAATCCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGc < 2:1758545/101‑1 (MQ=255)
aGGAATCCGGCCTGCGGCCGATCCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGTTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGc < 1:1354403/101‑1 (MQ=255)
ggAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAG.A.A.AAAAGGGGGTTGC.TTTTTTTTTTTCCCAAATATCCCCGGGG..GGG..ggggggg > 1:3975066/1‑99 (MQ=255)
gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAG.A.T.AAAAGGGGTTTTC.TTTTTTCTTTTCCCCAATATACCCGGGG..GGC..GGGGGGag > 2:5055449/1‑101 (MQ=255)
aaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCaga < 1:251233/101‑1 (MQ=255)
aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCagag < 2:4305019/101‑1 (MQ=255)
ttCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGCAAG.A.T.AAAGGGGCTTTTC.TTTTTTCTTTTCCCCAATATCCCCGGGG..TGG..ggggggcgagg > 2:408253/1‑95 (MQ=25)
gcgcccTGGGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAGGGGGTTTTGT.TTTTTTCTTCCCCAAAATATCCCGGGGG..TGC..GGGGGCAGAGcc < 2:1966955/97‑1 (MQ=21)
ggCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGGAAG.A.T.AAAGGGGCTTTGC.TTTTTTCTTTTCCCAAATATCCCGGGGG..GGC..GGGGGGAGAGcccc > 2:1512005/1‑101 (MQ=25)
ggCCTGCGGCCGATGCCTCCGGCAGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGcccc < 2:5699008/101‑1 (MQ=255)
gCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGccccc < 2:518088/101‑1 (MQ=255)
tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.GAA.ACCCATCCC.TTTCATCTTGCCCCAAATATCCCGGGGG..GCCCGGGGGGCAGCGcccccc < 1:3481020/94‑2 (MQ=25)
cTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCCcgc > 2:1691109/1‑101 (MQ=255)
cTGCGCCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC..TTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCCcgcc < 2:5371227/101‑2 (MQ=255)
ctttcGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.GAA.GCCCATCCC.TTTAATCTTGCCCCAAATATCCCGGGGG..ACCCGGGGGGCAGCGccccccgcc < 2:1873158/97‑5 (MQ=21)
ggCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGGTTTGC.TTTTATCTTTCCCCAAAAATCCCGGGGG..GGC..GGGGGGAGAGCCCCcgccgcc > 2:760795/1‑97 (MQ=25)
ttcGATGCCTCCGGCGGGGATATTTTGGGCAAG.A.T.GAA.AACCATCCC.CTTCATCTTGCCCCCAATATCCCGGGGG..GCCCGGGGGGCAGCGcccccccccac > 2:690298/3‑95 (MQ=21)
cGATGCCTCCGGCGGGGAAATTTTGGGCAAG.A.T.AAAGGGGGTTTTG.TTTTATCTTTCCCCAAATATCCCGGGGG..GGC..GGGGGCAGAGCCCCcgccgccacg > 2:5531468/1‑94 (MQ=21)
gATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.GAA.AACCATCCC.TTTCATCTTTCCCCAAATATCCCGGGGGGCCCC..GGGGGCAGCGcccccccccccctc > 2:4451393/1‑92 (MQ=18)
ccGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCCC.C.CCGCCCCGGGTCAcgc > 2:3526761/1‑101 (MQ=18)
gcggGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgccgc > 1:4692927/1‑101 (MQ=255)
gcggGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgccgc > 1:5236214/1‑101 (MQ=255)
cggGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg > 1:328963/1‑101 (MQ=255)
cggGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg > 2:3913489/1‑101 (MQ=255)
cggGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg > 1:5782886/1‑101 (MQ=255)
tatTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc > 1:2301844/1‑101 (MQ=255)
atTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct > 2:2759915/1‑101 (MQ=255)
atTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct > 2:4321621/1‑101 (MQ=255)
tttGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtctc > 1:48469/1‑101 (MQ=255)
tttGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtctc > 2:1681168/1‑101 (MQ=255)
ggggCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg > 2:3468972/1‑101 (MQ=255)
gggCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg > 1:649680/1‑101 (MQ=255)
cAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc > 2:1583243/1‑101 (MQ=255)
gggaaaaaaaaGGGGTCTTGT.TTTTTTTTTTCCCCAAA.ATCCCCGGGG..GGT..GGGGGGGAAGACCCCCAGCCGCCACGGTTCACGCCGCGACCGTCTCCggg < 1:1939306/93‑1 (MQ=255)
aaG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg > 1:5484766/1‑101 (MQ=255)
aaG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg > 2:101627/1‑101 (MQ=255)
aaG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg > 1:2337035/1‑101 (MQ=255)
aG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc > 2:2197728/1‑101 (MQ=255)
a.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa > 1:5598530/1‑101 (MQ=255)
t.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCGCGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg > 1:4965843/1‑101 (MQ=255)
t.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg > 2:5709579/1‑101 (MQ=255)
aaaGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGCCAgc > 1:4486715/1‑101 (MQ=255)
gggCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga > 1:1651199/1‑101 (MQ=255)
ggCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgac > 1:534776/1‑101 (MQ=255)
gctttgc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacg > 1:4196535/1‑101 (MQ=255)
tttgc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGat > 1:5105610/1‑101 (MQ=255)
tttgc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGat > 1:1773478/1‑101 (MQ=255)
ttgc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGata > 1:284711/1‑101 (MQ=255)
tgc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat > 1:3032641/1‑101 (MQ=255)
tgc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat > 2:3002694/1‑101 (MQ=255)
tgc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat > 1:938071/1‑101 (MQ=255)
gc.tttTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc > 1:831176/1‑101 (MQ=255)
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GCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.A.T.AAAGGGGCTTTGC.TTTTATCTTTCCCCAAATATCCCGGGGG..TGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATC > NC_014034/3439699‑3439901
Base quality scores: 
ATCG
< 3 ≤
ATCG
< 18 ≤
ATCG
< 27 ≤
ATCG
< 34 ≤
ATCG
< 41 ≤
ATCG
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