Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,531,604 T→C 100% A410A (GCT→GCC aceA → isocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,531,6040T→C100.0% 1066.3 284A410A (GCT→GCCaceAisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (146/138):  ref base (0/0):  total (146/138)

Only 100 of 320 total aligned reads displayed.

CTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCG  >  NC_014034/3531506‑3531701
                                                                                                  |                                                                                                 
 tGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTAt                                                                                                <  1:72978/101‑1 (MQ=255)
 tGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCAACGAAGACGCCTAt                                                                                                >  1:67764/1‑101 (MQ=255)
    ccTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCg                                                                                             >  2:178960/1‑101 (MQ=255)
     cTACAACAACTCGCCCAGCTTCACCTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGa                                                                                            <  1:1583772/101‑1 (MQ=255)
      tACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGaa                                                                                           >  1:4620523/1‑101 (MQ=255)
      tACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGaa                                                                                           >  1:2571631/1‑101 (MQ=255)
      tACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGaa                                                                                           <  1:5524173/101‑1 (MQ=255)
       acaacaACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAAc                                                                                          <  1:4769629/101‑1 (MQ=255)
       acaacaACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAAc                                                                                          <  2:2871278/101‑1 (MQ=255)
        caacaaCTCGCCCAGCTTCAACTGGACGCTTAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACg                                                                                         >  1:122928/1‑101 (MQ=255)
        caacaaCTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACg                                                                                         <  2:1228726/101‑1 (MQ=255)
         aacaacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACgg                                                                                        >  2:1935170/1‑101 (MQ=255)
         aacaacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACgg                                                                                        <  1:2126084/101‑1 (MQ=255)
          acaacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACggg                                                                                       >  2:4207641/1‑101 (MQ=255)
             acTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGccc                                                                                    <  1:379936/101‑1 (MQ=255)
              cTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCg                                                                                   >  2:2690620/1‑101 (MQ=255)
               tCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGTCGCCTATCCGAACGGGCCCGa                                                                                  <  1:4259930/101‑1 (MQ=255)
               tCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGa                                                                                  <  2:1538184/101‑1 (MQ=255)
                cGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAg                                                                                 <  2:1714597/101‑1 (MQ=255)
                cGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAg                                                                                 >  2:4012007/1‑101 (MQ=255)
                 gCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGc                                                                                <  1:4482897/101‑1 (MQ=255)
                  cccAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCt                                                                               >  1:858034/1‑101 (MQ=255)
                   ccAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCAGCGAAGACGCCTATCCGAACGGGCCCGAGCtg                                                                              <  1:5152674/101‑1 (MQ=255)
                   ccAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtg                                                                              <  1:3081339/101‑1 (MQ=255)
                   ccAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtg                                                                              >  2:1613776/1‑101 (MQ=255)
                    cAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtga                                                                             >  2:3638694/1‑101 (MQ=255)
                     aGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGGGCGCGCGCAGTGGCTGGCCGAGGGCACCATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgat                                                                            >  1:3483435/1‑101 (MQ=255)
                     aGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGCGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACTGGCCCGAGCtgat                                                                            >  1:3835566/1‑101 (MQ=255)
                      gCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgatg                                                                           <  1:1085421/101‑1 (MQ=255)
                       cTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgatga                                                                          <  1:5543968/101‑1 (MQ=255)
                         tCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAg                                                                        <  2:5615067/101‑1 (MQ=255)
                          cAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGc                                                                       <  1:933424/101‑1 (MQ=255)
                          cAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGc                                                                       <  2:4864718/101‑1 (MQ=255)
                           aaCTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGcc                                                                      <  2:2740451/101‑1 (MQ=255)
                               ggACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGAc                                                                  <  1:656767/101‑1 (MQ=255)
                               ggACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGAc                                                                  >  2:2970529/1‑101 (MQ=255)
                                    cTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATga                                                             <  2:4409175/101‑1 (MQ=255)
                                    cTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATga                                                             <  1:1699767/101‑1 (MQ=255)
                                     tGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgac                                                            <  2:856811/101‑1 (MQ=255)
                                      gAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacg                                                           >  1:3225094/1‑101 (MQ=255)
                                      gAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacg                                                           >  2:2494908/1‑101 (MQ=255)
                                      gAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacg                                                           >  1:3557993/1‑101 (MQ=255)
                                       aaCCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacga                                                          >  2:5318935/1‑101 (MQ=255)
                                       aaCCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacga                                                          >  2:1085095/1‑101 (MQ=255)
                                       aaCCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacga                                                          <  2:2450512/101‑1 (MQ=255)
                                        aCCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacgac                                                         >  2:2279911/1‑101 (MQ=255)
                                         ccTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGTCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGaca                                                        <  2:5005178/101‑1 (MQ=255)
                                          cTGCGCAAGCAGGTGCGCTCGCATTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTTTCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGacac                                                       <  1:4880195/101‑1 (MQ=255)
                                          cTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGacac                                                       >  2:2200833/1‑101 (MQ=255)
                                          cTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGacac                                                       >  2:715610/1‑101 (MQ=255)
                                           tGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACg                                                      >  1:3740291/1‑101 (MQ=255)
                                            gcgcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGc                                                     <  2:3106727/101‑1 (MQ=255)
                                               cAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccc                                                  >  1:5362208/1‑100 (MQ=255)
                                               cAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccc                                                  <  2:3531512/101‑2 (MQ=255)
                                               cAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccc                                                  <  2:5143210/101‑2 (MQ=255)
                                                aaGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccct                                                 <  2:657041/101‑3 (MQ=255)
                                                aaGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccct                                                 <  1:2682906/101‑3 (MQ=255)
                                                    aGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCCCCGAAGGCGCCTATCCGCACGGGCCCGCGCTGATGAAGCCCGACTATGACGACACCCCCCTCGGc                                             >  1:1610017/1‑101 (MQ=255)
                                                          gcgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGaa                                       <  2:206220/101‑1 (MQ=255)
                                                          gcgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGaa                                       >  2:5089002/1‑101 (MQ=255)
                                                           cgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAg                                      <  1:4207901/101‑1 (MQ=255)
                                                            gcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGc                                     <  1:110605/101‑1 (MQ=255)
                                                            gcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGc                                     <  2:3224894/101‑1 (MQ=255)
                                                             cgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAGGCCAGACTATGACGACACGCCCCTCGGCCGCGAAGcc                                    >  1:4956142/1‑101 (MQ=255)
                                                                ggTGGCTGGCCAAGGGCACGATCCCCGAAGCCGCCTATCCGAACGGGCCCGAGCTAATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGAt                                 <  1:2001761/100‑1 (MQ=255)
                                                                aggggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGAt                                 <  2:4483544/98‑1 (MQ=255)
                                                                 gTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATg                                >  2:2131101/1‑101 (MQ=255)
                                                                 gTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATg                                >  1:150515/1‑101 (MQ=255)
                                                                  gggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGc                               <  2:4422983/100‑1 (MQ=255)
                                                                     ctggcCGAGGGCAGGACCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                            <  1:4396100/101‑1 (MQ=255)
                                                                     ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                            <  1:643927/101‑1 (MQ=255)
                                                                     ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                            >  1:4017105/1‑101 (MQ=255)
                                                                     ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                            >  2:1323848/1‑101 (MQ=255)
                                                                          cGAGGGCACGATCACCGAAGCCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                       <  2:4293295/101‑1 (MQ=255)
                                                                            aGGGCACGATCGCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                     <  1:2717664/101‑1 (MQ=255)
                                                                            aGGGCACGATCACCGAAGACGCCTATCCGACCGGCCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                     <  2:4620247/101‑1 (MQ=255)
                                                                            aGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                     >  2:5257798/1‑101 (MQ=255)
                                                                             gggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAgg                    >  1:1029851/1‑101 (MQ=255)
                                                                             gggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAgg                    <  1:1321985/101‑1 (MQ=255)
                                                                             gggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAgg                    >  1:3962650/1‑101 (MQ=255)
                                                                             gggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCCGATGAAGCCCAACTATGACGACACGCCCCTCGGCCGCGAAGCCTGTGCCTGGCTCAAgg                    >  2:3826653/1‑101 (MQ=255)
                                                                               gCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGcc                  >  2:3323515/1‑101 (MQ=255)
                                                                               gCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGcc                  >  1:5270202/1‑101 (MQ=255)
                                                                                cACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCt                 <  2:5564596/101‑1 (MQ=255)
                                                                                  cGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTc               <  2:4129532/101‑1 (MQ=255)
                                                                                  cGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTc               >  1:2441883/1‑101 (MQ=255)
                                                                                   gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc              >  1:2349402/1‑101 (MQ=255)
                                                                                   gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc              >  1:2476669/1‑101 (MQ=255)
                                                                                    aTCACCGAAGACCCCTACCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCa             <  2:150495/101‑1 (MQ=255)
                                                                                     tCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg            <  2:5269912/101‑1 (MQ=255)
                                                                                     tCACCGAAGACCCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg            <  2:2441749/101‑1 (MQ=255)
                                                                                      cACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGa           <  1:5110341/101‑1 (MQ=255)
                                                                                      cACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGa           >  2:595793/1‑101 (MQ=255)
                                                                                       cccGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc          <  2:89781/100‑1 (MQ=255)
                                                                                        ccGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc         >  1:3372521/1‑101 (MQ=255)
                                                                                         cGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCg        >  1:753581/1‑101 (MQ=255)
                                                                                          gAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCga       <  1:4163425/101‑1 (MQ=255)
                                                                                           aaGACGCCTATCCGACCGGGCCCGAGCTGATGAACCCCCACTATGACGCCACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgag      <  1:1741889/101‑1 (MQ=255)
                                                                                              aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc   <  1:2243864/101‑1 (MQ=255)
                                                                                              aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc   <  1:2200955/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCG  >  NC_014034/3531506‑3531701

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG