Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,531,673 G→C 100% A433A (GCG→GCC aceA → isocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,531,6730G→C100.0% 1160.8 299A433A (GCG→GCCaceAisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (162/137):  ref base (0/0):  total (162/137)

Only 100 of 307 total aligned reads displayed.

CTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCGATGG  >  NC_014034/3531575‑3531770
                                                                                                  |                                                                                                 
ctggcCGAGGGCAGGACCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                                                                                                 <  1:4396100/101‑1 (MQ=255)
ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                                                                                                 >  1:4017105/1‑101 (MQ=255)
ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                                                                                                 <  1:643927/101‑1 (MQ=255)
ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                                                                                                 >  2:3419857/1‑101 (MQ=255)
   gcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCt                                                                                              >  1:963163/1‑101 (MQ=255)
    ccGAGGCCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTc                                                                                             <  1:709555/101‑1 (MQ=255)
     cGAGGGCACGATCACCGAAGCCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                                                                                            <  2:4293295/101‑1 (MQ=255)
      gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                                                                                           >  1:2211/1‑101 (MQ=255)
       aGGGCACGATCGCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                                                                                          <  1:2717664/101‑1 (MQ=255)
       aGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                                                                                          >  2:5257798/1‑101 (MQ=255)
        gggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAgg                                                                                         >  2:1193414/1‑101 (MQ=255)
        gggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAgg                                                                                         >  1:3962650/1‑101 (MQ=255)
        gggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCCGATGAAGCCCAACTATGACGACACGCCCCTCGGCCGCGAAGCCTGTGCCTGGCTCAAgg                                                                                         >  2:3826653/1‑101 (MQ=255)
         ggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCCCAAGGc                                                                                        >  1:5170377/1‑101 (MQ=255)
           cACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCt                                                                                      <  2:5564596/101‑1 (MQ=255)
            aCGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCtt                                                                                     <  1:1464176/101‑1 (MQ=255)
            aCGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCtt                                                                                     >  1:4320917/1‑101 (MQ=255)
             cGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTc                                                                                    <  2:4129532/101‑1 (MQ=255)
              gATCCCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                                                   <  1:3488486/101‑1 (MQ=255)
              gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                                                   >  1:40455/1‑101 (MQ=255)
                tCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg                                                                                 <  2:5269912/101‑1 (MQ=255)
                tCACCGAAGACCCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg                                                                                 <  2:2441749/101‑1 (MQ=255)
                 cACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGa                                                                                <  1:5110341/101‑1 (MQ=255)
                   ccGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc                                                                              >  1:3372521/1‑101 (MQ=255)
                   ccGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc                                                                              <  2:517636/101‑1 (MQ=255)
                    cGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCg                                                                             <  1:3254392/101‑1 (MQ=255)
                    acgtgtcGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCg                                                                             >  2:536311/7‑101 (MQ=255)
                     gAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCga                                                                            <  1:4163425/101‑1 (MQ=255)
                      aaGACGCCTATCCGACCGGGCCCGAGCTGATGAACCCCCACTATGACGCCACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgag                                                                           <  1:1741889/101‑1 (MQ=255)
                      aaGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgag                                                                           <  1:1613870/101‑1 (MQ=255)
                        gccGCCTACCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCCCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGAt                                                                         <  1:5057701/99‑1 (MQ=255)
                        gACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGAt                                                                         <  2:5642104/101‑1 (MQ=255)
                         aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc                                                                        <  2:4446899/101‑1 (MQ=255)
                         aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc                                                                        <  1:3118515/101‑1 (MQ=255)
                         aCGCCTATCCAACCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc                                                                        <  2:207959/101‑1 (MQ=255)
                           gcctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcgc                                                                      <  2:855503/98‑1 (MQ=255)
                           gcctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcgc                                                                      >  1:3740602/4‑101 (MQ=255)
                            cctacccGAACGGGCCCGAGCTGATGAAGCCCGCCTACGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcgcg                                                                     <  1:4941247/96‑1 (MQ=255)
                            cctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcgcg                                                                     >  1:5266502/3‑101 (MQ=255)
                             ctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGa                                                                    <  1:2632024/100‑1 (MQ=255)
                             ctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGa                                                                    >  2:2462237/2‑101 (MQ=255)
                               aTCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAcg                                                                  <  1:2003058/101‑1 (MQ=255)
                                 ccGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAcgcg                                                                <  2:40444/101‑1 (MQ=255)
                                  cGAAGGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgg                                                               <  2:1609923/101‑1 (MQ=255)
                                  cGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgg                                                               <  2:2922266/101‑1 (MQ=255)
                                   gAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcggg                                                              >  2:4582506/1‑101 (MQ=255)
                                    aaCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGCGATCGCTACgcgggc                                                             >  2:2540380/1‑101 (MQ=255)
                                    aaCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggc                                                             >  2:2260671/1‑101 (MQ=255)
                                    aaCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggc                                                             <  1:1323936/101‑1 (MQ=255)
                                       gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                                          >  2:3511056/1‑101 (MQ=255)
                                        ggCCCGGGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCgggg                                                         <  1:2138912/101‑1 (MQ=255)
                                        ggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCgggg                                                         <  1:1018330/101‑1 (MQ=255)
                                          cccGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgt                                                       >  2:127252/1‑101 (MQ=255)
                                             gAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGtt                                                    <  1:2495050/101‑1 (MQ=255)
                                              aGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTc                                                   >  1:4806232/1‑101 (MQ=255)
                                                cTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCca                                                 <  2:604237/101‑1 (MQ=255)
                                                 tgatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcac                                                >  1:3619890/1‑101 (MQ=255)
                                                 tgatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcac                                                >  1:733598/1‑101 (MQ=255)
                                                 tgatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcac                                                >  2:1825595/1‑101 (MQ=255)
                                                     gaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAAcc                                            >  2:1361964/1‑101 (MQ=255)
                                                      aaGCCCGACTATGCCGACACGCCCCCCGGCCGTGAAGCCGATGCCTGGCTCAAGGCCTTCCAGCCCGAGATCGCGACGCGGGCGGGGGTGTTCCACACCCt                                           <  2:2676821/101‑1 (MQ=255)
                                                            gACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAc                                     <  1:2177021/101‑1 (MQ=255)
                                                             aCTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAcc                                    >  2:1728776/1‑101 (MQ=255)
                                                              cTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAccc                                   >  1:5510053/1‑101 (MQ=255)
                                                                aTGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTg                                 <  1:1825705/101‑1 (MQ=255)
                                                                 tGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGc                                >  1:2116594/1‑101 (MQ=255)
                                                                 tGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGc                                >  2:24916/1‑101 (MQ=255)
                                                                  gacgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGcc                               >  1:4447978/1‑101 (MQ=255)
                                                                   acgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGccc                              <  1:2280037/101‑1 (MQ=255)
                                                                   acgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGccc                              <  1:4601288/101‑1 (MQ=255)
                                                                    cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCa                             >  1:2462027/1‑101 (MQ=255)
                                                                      acacGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCAcc                           <  1:619045/101‑1 (MQ=255)
                                                                       cacGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCt                          <  2:277389/101‑1 (MQ=255)
                                                                         cGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTc                        >  2:3979425/1‑101 (MQ=255)
                                                                         cGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTc                        <  2:3738310/101‑1 (MQ=255)
                                                                           cccCTCGGGCGCGATGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCa                      <  1:4997703/101‑1 (MQ=255)
                                                                           cccCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCa                      >  1:1184010/1‑101 (MQ=255)
                                                                             cctCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATc                    >  2:1970947/3‑101 (MQ=255)
                                                                             cctCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATc                    >  2:4479847/3‑101 (MQ=255)
                                                                               tCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTg                  <  1:5286722/101‑1 (MQ=255)
                                                                               ccGGCCCGGAAGGCGATGCCCTGCTCAAGGCCTTTCAGGCCCAAATTGCGGCGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTg                  <  2:1987051/100‑1 (MQ=255)
                                                                                cGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGa                 <  2:683832/101‑1 (MQ=255)
                                                                                cGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGa                 >  1:3814928/1‑101 (MQ=255)
                                                                                cGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGa                 >  1:2357912/1‑101 (MQ=255)
                                                                                 ggCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACCACCTGATCACCCTGCCCACCTTCCATCTgcc                >  2:3902474/1‑99 (MQ=255)
                                                                                 ggCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGAc                <  1:5603132/101‑1 (MQ=255)
                                                                                 ggCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGAc                <  2:4949199/101‑1 (MQ=255)
                                                                                 ggCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGAc                >  2:3129994/1‑101 (MQ=255)
                                                                                 ggCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGAc                >  2:4381781/1‑101 (MQ=255)
                                                                                  gCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGAcc               <  2:3265539/101‑1 (MQ=255)
                                                                                    cgcgAAGCCGATGCCTGGCGCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCGCCTTCCATCTGACcgc             <  2:875950/101‑1 (MQ=255)
                                                                                     gcgAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACcgcg            >  2:4877151/1‑101 (MQ=255)
                                                                                      cgAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGa           <  2:1096170/101‑1 (MQ=255)
                                                                                       gAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGaa          >  2:5500011/1‑101 (MQ=255)
                                                                                       gAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGaa          >  1:835453/1‑101 (MQ=255)
                                                                                         aGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAAt        <  2:1468155/101‑1 (MQ=255)
                                                                                         aGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAAt        <  1:2965814/101‑1 (MQ=255)
                                                                                              aTGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgatg   >  2:5317550/1‑101 (MQ=255)
                                                                                               tGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgatgg  >  2:4637152/1‑101 (MQ=255)
                                                                                               tGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGAGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgatgg  <  2:2309772/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCGATGG  >  NC_014034/3531575‑3531770

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG