Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,479 G→A 3.6% intergenic (+82/+66) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4790G→A 3.6% 12.0 ‑1.9 ‑0.1222intergenic (+82/+66)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (6/2):  ref base (100/114):  total (106/116)
Fisher's exact test strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.53e-01

Only 100 of 249 total aligned reads displayed.

GCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGT  >  NC_014034/432380‑432579
                                                                                                     |                                                                                                    
gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCg                                                                                                      >  1:1690151/1‑101 (MQ=255)
 cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGATGCAATATGAAAGCCG.AACCGGGCTTCGt                                                                                                     >  2:421536/1‑101 (MQ=21)
 cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGt                                                                                                     <  1:5505020/101‑1 (MQ=255)
   gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGttt                                                                                                   <  1:2798056/101‑1 (MQ=255)
    cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTc                                                                                                  <  1:331890/101‑1 (MQ=255)
      ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCAt                                                                                                <  1:1408847/101‑1 (MQ=255)
      ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCAt                                                                                                <  2:2210739/101‑1 (MQ=255)
              atGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGcccc                                                                                        >  2:3294165/2‑101 (MQ=255)
                gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCCGGGTATTTGAGGCAAGATGAAAACCG.ATCCGGGGTTCGTTTCTTTTTTccccca                                                                                      >  1:3946796/1‑99 (MQ=17)
                 aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCaaa                                                                                     <  2:5573872/101‑1 (MQ=255)
                 aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCaaa                                                                                     <  1:3178902/101‑1 (MQ=255)
                  ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAat                                                                                    <  1:91761/101‑1 (MQ=255)
                  ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAat                                                                                    <  2:4677033/101‑1 (MQ=255)
                    ccGGGCAGGGGGGCGCTTCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAatat                                                                                  <  2:5202594/101‑1 (MQ=255)
                     cGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATc                                                                                 <  2:4545255/101‑1 (MQ=255)
                     cGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATc                                                                                 <  2:14140/101‑1 (MQ=255)
                      gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGAATTTGGGTCAAGATGAAAGCCG.ATGCGGGCTTCGGTTCATCTTGCCCCCAATAAcc                                                                                >  1:3983753/1‑101 (MQ=18)
                      gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATAGTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTTATCTTGGCCCGAATATcc                                                                                >  2:1591596/1‑101 (MQ=18)
                       ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAtt                                                                    >  2:4111636/5‑101 (MQ=2)
                       ggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCCAATATccc                                                                               >  1:4360376/1‑101 (MQ=255)
                       ggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATccc                                                                               <  1:3721795/101‑1 (MQ=255)
                        gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                                              <  1:1184968/101‑1 (MQ=255)
                         gcggggggCTCTGCCCCCCGG.CTTC..TCTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttgg                                                                  <  1:2690237/99‑3 (MQ=2)
                         acggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttgg                                                                  >  1:5550287/3‑99 (MQ=2)
                          cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc                                                                 >  1:481468/2‑98 (MQ=2)
                          cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc                                                                 >  2:135129/2‑98 (MQ=2)
                          cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc                                                                 >  2:4762476/2‑98 (MQ=2)
                          cgggggaCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc                                                                 <  1:923282/100‑4 (MQ=2)
                          aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                                            >  1:4243759/1‑101 (MQ=255)
                          aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                                            <  1:192117/101‑1 (MQ=255)
                          aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                                            <  2:3316219/101‑1 (MQ=255)
                           ggggggGCCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                                                                           <  1:767491/101‑1 (MQ=25)
                             ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGt                                                                         <  2:4932296/101‑1 (MQ=255)
                              gggCGCTGCCCCCCGTCCTGCGGCCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                                        <  1:2143756/101‑1 (MQ=255)
                              gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                                        <  2:3612923/101‑1 (MQ=255)
                              gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAAGCG.ATCCGGGCTTCGTTTCATCTTGCCCCCAATATCCCcgggggg                                                                        >  1:1046620/1‑99 (MQ=21)
                                gcgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGaa                                                                      <  2:4454017/101‑1 (MQ=255)
                                  gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtt                                                                    <  1:4504679/101‑1 (MQ=255)
                                   cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                                                                   >  1:4494171/1‑101 (MQ=255)
                                    tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                  >  2:2755537/1‑101 (MQ=255)
                                    tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                  <  1:5046345/101‑1 (MQ=255)
                                    tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                  <  1:3567012/101‑1 (MQ=255)
                                    tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAAtttt                                                                  >  1:4643210/1‑101 (MQ=255)
                                     gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg                                                                 <  2:311251/101‑1 (MQ=255)
                                     gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg                                                                 <  2:2364026/101‑1 (MQ=255)
                                        ccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGgcg                                                              <  2:5673966/101‑1 (MQ=255)
                                         cccGTCCTGCGGACTCCCCCCGGGGAATTTTAGGGAAAAAGAAAACCGAACCCGGGCTTCGTTTCTTTTTCCCCCAAAATCCCCGGGGGGGATttttttgc                                                              >  1:16629/1‑97 (MQ=255)
                                            gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                          <  2:692784/98‑1 (MQ=255)
                                              ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                        >  1:4616774/2‑101 (MQ=255)
                                              ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                        <  2:1797681/100‑1 (MQ=255)
                                              ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                        >  2:4313518/2‑101 (MQ=255)
                                                tGCGGCCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa                                                      <  2:3983509/101‑1 (MQ=255)
                                                 gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                                     <  2:584005/101‑1 (MQ=255)
                                                 gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                                     >  2:731983/1‑101 (MQ=255)
                                                  cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                                                    <  2:3530530/101‑1 (MQ=255)
                                                  cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                                                    <  1:5398775/101‑1 (MQ=255)
                                                     aCTCCCCCCGGGATATTTTAGGCAAGATGAAAAGCG.AACCGGGGTTCGTTTTATCTTGCCCCCAAAATTCCGGGGGGGAATTTTTGCGCCGCCCAAAGAgg                                                 >  2:1077342/1‑101 (MQ=16)
                                                     aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                                 <  1:1342282/101‑1 (MQ=255)
                                                        ccccccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggggg                                              <  1:4166356/101‑1 (MQ=255)
                                                          ccccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                            <  1:656074/101‑1 (MQ=255)
                                                           cccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                           >  1:1564226/1‑101 (MQ=255)
                                                           cccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                           >  2:3482380/1‑101 (MQ=255)
                                                           cccGGGATATTTGAGGCAAGACGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                           <  2:2851708/101‑1 (MQ=255)
                                                           cccGGGATATCTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                           <  2:120476/101‑1 (MQ=255)
                                                            ccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                          >  2:5470209/1‑101 (MQ=255)
                                                            ccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                          <  2:213266/101‑1 (MQ=255)
                                                            ccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                          >  2:2941720/1‑101 (MQ=255)
                                                            ccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                          <  2:563656/101‑1 (MQ=255)
                                                              gggATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                                        >  1:2400090/1‑101 (MQ=255)
                                                               gtatatTTGAGGCAACAGCAAAGGCG.ATCCGGGCCTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc                                       <  1:2509068/99‑1 (MQ=21)
                                                               ggATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc                                       >  1:5280629/1‑101 (MQ=255)
                                                                gATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                      <  1:1827960/101‑1 (MQ=255)
                                                                gATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                      >  2:3563759/1‑101 (MQ=255)
                                                                 atatTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                                     >  1:2048893/1‑101 (MQ=255)
                                                                 atatTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                                     <  2:1761182/101‑1 (MQ=255)
                                                                 atatTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                                     >  1:3424483/1‑101 (MQ=255)
                                                                 atatTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                                     >  1:2569207/1‑101 (MQ=255)
                                                                  tatTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                                    <  2:5125877/101‑1 (MQ=255)
                                                                  tatTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                                    >  1:3156795/1‑101 (MQ=255)
                                                                   atTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                   >  1:447670/1‑101 (MQ=255)
                                                                    tttGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                                  >  1:1487314/1‑101 (MQ=255)
                                                                     ttGAGGCAAGATGAAAGCCG.GTCCGGGCTTCGTTTCATCTTGCCCCAAATTTCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                                 <  1:2412119/101‑1 (MQ=255)
                                                                     tggAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                                 <  2:1968849/99‑1 (MQ=255)
                                                                      tGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                                >  1:5006292/1‑101 (MQ=255)
                                                                        aGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTTAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                              <  2:1690052/101‑1 (MQ=255)
                                                                          gCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                            >  2:4994585/1‑101 (MQ=255)
                                                                          gCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                            <  2:245172/101‑1 (MQ=255)
                                                                            aaGTTGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg                          >  2:2662522/1‑101 (MQ=255)
                                                                            aaGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg                          >  1:4293007/1‑101 (MQ=255)
                                                                            aaGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg                          <  1:5561719/101‑1 (MQ=255)
                                                                                 gAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg                     <  2:1571886/101‑1 (MQ=255)
                                                                                 gAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg                     >  1:5083847/1‑101 (MQ=255)
                                                                                  aaaGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg                    >  2:3807944/1‑101 (MQ=255)
                                                                                   aaGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggccgcgc                   >  1:1027197/1‑96 (MQ=255)
                                                                                   aaGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc                   >  1:5579080/1‑101 (MQ=255)
                                                                                          ctCCGGGCTTCGGTTCATCTTGCCCCAAATATCGCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg             <  2:2617051/100‑1 (MQ=255)
                                                                                          aTCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg             >  2:4028998/1‑101 (MQ=255)
                                                                                            ccGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCggcgcggcggcgcggc           >  2:486178/1‑101 (MQ=255)
                                                                                             cGGGCTTCGTTTCATCTTGCCGCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGAGGCAGCGCCCCCTATCCCCGCGgcgcggcggcgcggcg          >  1:3276633/1‑101 (MQ=255)
                                                                                                     gTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGt  >  1:585809/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGT  >  NC_014034/432380‑432579

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG