Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,493 C→G 3.6% intergenic (+96/+52) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4930C→G 3.6% 8.9 ‑1.3 ‑0.0223intergenic (+96/+52)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (6/2):  ref base (107/108):  total (113/110)
Fisher's exact test strand distribution p-value = 2.80e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.92e-01

Only 100 of 250 total aligned reads displayed.

CTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGC  >  NC_014034/432394‑432593
                                                                                                    |                                                                                                    
cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                                                      <  1:1656112/101‑1 (MQ=255)
  gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCaa                                                                                                    >  1:1304300/1‑101 (MQ=255)
  gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCCGGGTATTTGAGGCAAGATGAAAACCGATCCGGGGTTCGTTTCTTTTTTccccca                                                                                                    >  1:3946796/1‑99 (MQ=17)
   aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCaaa                                                                                                   <  1:3178902/101‑1 (MQ=255)
    ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAat                                                                                                  <  1:91761/101‑1 (MQ=255)
      ccGGGCAGGGGGGCGCTTCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAatat                                                                                                <  2:5202594/101‑1 (MQ=255)
      ccGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAatat                                                                                                <  2:4427269/101‑1 (MQ=255)
       cGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATc                                                                                               <  2:4545255/101‑1 (MQ=255)
        gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATcc                                                                                              >  1:692841/1‑101 (MQ=255)
         ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAtt                                                                                  >  2:2125221/5‑101 (MQ=2)
         ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAtt                                                                                  >  2:4111636/5‑101 (MQ=2)
         ggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCCAATATccc                                                                                             >  1:4360376/1‑101 (MQ=255)
         ggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATccc                                                                                             <  1:3721795/101‑1 (MQ=255)
         ggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATccc                                                                                             <  1:2231118/101‑1 (MQ=255)
          gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                                                            <  1:1184968/101‑1 (MQ=255)
           cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgg                                                                                           <  2:2820545/101‑1 (MQ=255)
           cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgg                                                                                           >  1:1485863/1‑101 (MQ=255)
            cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc                                                                               >  2:4762476/2‑98 (MQ=2)
            cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc                                                                               >  2:135129/2‑98 (MQ=2)
            cgggggaCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc                                                                               <  1:923282/100‑4 (MQ=2)
            aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                                                          <  1:192117/101‑1 (MQ=255)
             ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                                                                                         >  1:2562656/1‑101 (MQ=255)
                gggCGCTGCCCCCCGTCCTGCGGCCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                                                      <  1:2143756/101‑1 (MQ=255)
                gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                                                      <  2:3612923/101‑1 (MQ=255)
                gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAAGCGATCCGGGCTTCGTTTCATCTTGCCCCCAATATCCCcgggggg                                                                                      >  1:1046620/1‑99 (MQ=21)
                 ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCCAGATGAAAGCCGATCCGGGCTNCGNTNCNTCTTGCCCCCANTATCCCGGGGGGGa                                                                                     >  1:4984814/1‑101 (MQ=21)
                    gcTCCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtt                                                                                  <  2:151496/101‑1 (MQ=255)
                     cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                                                                                 >  1:4494171/1‑101 (MQ=255)
                      tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                                <  1:5011224/101‑1 (MQ=255)
                      tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                                >  1:4142499/1‑101 (MQ=255)
                      tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                                <  1:3567012/101‑1 (MQ=255)
                      tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                                >  2:2471759/1‑101 (MQ=255)
                       gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg                                                                               <  2:177168/101‑1 (MQ=255)
                       gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg                                                                               <  1:4698144/101‑1 (MQ=255)
                       gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg                                                                               <  2:311251/101‑1 (MQ=255)
                       gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAATTTTg                                                                               >  1:4560098/1‑101 (MQ=255)
                        ccccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTgg                                                                              >  1:1761281/1‑101 (MQ=255)
                             cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAg                                                                         >  1:3345161/5‑101 (MQ=255)
                              gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                                        <  2:2874959/98‑1 (MQ=255)
                              gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                                        <  2:692784/98‑1 (MQ=255)
                                ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                                      >  2:4313518/2‑101 (MQ=255)
                                ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                                      >  1:4616774/2‑101 (MQ=255)
                                  tGCGGCCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa                                                                    <  2:3983509/101‑1 (MQ=255)
                                   gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                                                   <  2:584005/101‑1 (MQ=255)
                                    cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                                                                  <  1:2041246/101‑1 (MQ=255)
                                    cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                                                                  <  1:5398775/101‑1 (MQ=255)
                                    cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                                                                  <  2:3530530/101‑1 (MQ=255)
                                    cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCAAAag                                                                  >  1:5604183/1‑101 (MQ=255)
                                       aCTCCCCCCGGGATATTTTAGGCAAGATGAAAAGCGAACCGGGGTTCGTTTTATCTTGCCCCCAAAATTCCGGGGGGGAATTTTTGCGCCGCCCAAAGAgg                                                               >  2:1077342/1‑101 (MQ=16)
                                            ccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                                          <  2:3313220/101‑1 (MQ=255)
                                            ccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                                          <  1:656074/101‑1 (MQ=255)
                                             cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                                         >  2:3482380/1‑101 (MQ=255)
                                             cccGGGATATCTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                                         <  2:120476/101‑1 (MQ=255)
                                              ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                                        <  1:2672692/101‑1 (MQ=255)
                                              ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                                        >  2:5470209/1‑101 (MQ=255)
                                              ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                                        <  2:563656/101‑1 (MQ=255)
                                               cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                                       >  2:4613932/1‑101 (MQ=255)
                                                 ggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc                                                     >  1:5280629/1‑101 (MQ=255)
                                                  gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                                    >  2:3563759/1‑101 (MQ=255)
                                                   atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                                                   <  2:1761182/101‑1 (MQ=255)
                                                    tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                                                  >  1:3156795/1‑101 (MQ=255)
                                                    tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                                                  >  1:248602/1‑101 (MQ=255)
                                                    tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                                                  <  2:5125877/101‑1 (MQ=255)
                                                    tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                                                  >  1:5064942/1‑101 (MQ=255)
                                                     atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                                 >  1:1663798/1‑101 (MQ=255)
                                                      tttGAGGAAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                                                >  2:5667116/1‑101 (MQ=255)
                                                       tggAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                                               <  2:1968849/99‑1 (MQ=255)
                                                       tggAGGAAAGATGAAGGCCGATCCGGGCTGCGTTTCATCTTGCGCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCTCCCCCTAt                                               <  2:159711/99‑1 (MQ=21)
                                                           ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                                           <  1:2404245/101‑1 (MQ=255)
                                                            gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                                          <  1:1560460/101‑1 (MQ=255)
                                                              aaGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg                                        >  1:4293007/1‑101 (MQ=255)
                                                              aaGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg                                        <  1:5561719/101‑1 (MQ=255)
                                                               aGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg                                       >  2:2697940/1‑101 (MQ=255)
                                                                   gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg                                   <  2:1571886/101‑1 (MQ=255)
                                                                    aaaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg                                  >  2:3807944/1‑101 (MQ=255)
                                                                     aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggccgcgc                                 >  1:1027197/1‑96 (MQ=255)
                                                                     aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc                                 >  1:5553717/1‑101 (MQ=255)
                                                                      aGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg                                <  2:3137237/101‑1 (MQ=255)
                                                                         cGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcg                             <  2:4949094/101‑1 (MQ=255)
                                                                          gATCCGGGCTTCGTTTCATCTTGCCCCAAATGTCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc                            >  1:5125998/1‑101 (MQ=255)
                                                                           aTCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg                           >  2:4028998/1‑101 (MQ=255)
                                                                           aTCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg                           >  2:2183646/1‑101 (MQ=255)
                                                                             ccGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCggcgcggcggcgcggc                         >  2:486178/1‑101 (MQ=255)
                                                                              cGGGCTTCGTTTCATCTTGCCGCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGAGGCAGCGCCCCCTATCCCCGCGgcgcggcggcgcggcg                        >  1:3276633/1‑101 (MQ=255)
                                                                              cGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGgcgcggcggcgcggcg                        >  1:4175124/1‑101 (MQ=255)
                                                                               gggCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCggcgcggcgc                       >  2:2248426/1‑101 (MQ=255)
                                                                                   ttCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGcc                   >  1:4481906/1‑101 (MQ=255)
                                                                                      gTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGt                >  2:261969/1‑101 (MQ=255)
                                                                                       tttCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTc               >  1:5774087/1‑101 (MQ=255)
                                                                                        ttCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCg              >  1:1818511/1‑101 (MQ=255)
                                                                                        ttCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGAGCGGCGCAGCCGGTCg              >  1:271729/1‑101 (MQ=255)
                                                                                         tCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGt             >  2:4928725/1‑101 (MQ=255)
                                                                                          cATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGtt            >  1:4811849/1‑101 (MQ=255)
                                                                                          cATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGGCGtt            >  1:2390098/1‑101 (MQ=255)
                                                                                           aTCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTg           <  2:2416641/101‑1 (MQ=255)
                                                                                            tCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGa          <  1:3482597/101‑1 (MQ=255)
                                                                                              ttGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCTGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATc        <  2:557275/101‑1 (MQ=255)
                                                                                               tGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcg       >  2:3336337/1‑101 (MQ=255)
                                                                                                  cccAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgcgc    <  2:1680060/101‑1 (MQ=255)
                                                                                                   ccAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgcgcg   >  2:1412077/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGC  >  NC_014034/432394‑432593

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG