Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 445,294 G→T 100% G168V (GGC→GTC)  RCAP_rcc00417 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034445,2940G→T100.0% 1160.8 301G168V (GGC→GTC) RCAP_rcc00417hypothetical protein
Reads supporting (aligned to +/- strand):  new base (137/164):  ref base (0/0):  total (137/164)

Only 100 of 309 total aligned reads displayed.

GGTGGCGGTGCTGGGCGTGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGGCTTTGCGCTGGCCTCGGTTTTGGTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCGGTGCTG  >  NC_014034/445196‑445391
                                                                                                  |                                                                                                 
ggtggcggtgCTGGGCGTGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCt                                                                                                 >  2:4463149/1‑101 (MQ=255)
     cggtgCTGGGCGTGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTAAGGGCTGCCGGTGGCCTCGATGCTGGTCTTTgcg                                                                                            >  1:4926420/1‑101 (MQ=255)
      ggtgCTGGGCGTGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTgcgc                                                                                           >  2:2363943/1‑101 (MQ=255)
        tgCTGGGCGTGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGGGCTg                                                                                         >  2:2577410/1‑101 (MQ=255)
        tgCTGGGCGTGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTg                                                                                         <  2:2394731/101‑1 (MQ=255)
        tgCTGGGCGTGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTg                                                                                         >  1:2972410/1‑101 (MQ=255)
        tgCTGGGCGTGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTg                                                                                         <  2:1612130/101‑1 (MQ=255)
        tgCTGGGCGTGCTCATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGTCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTg                                                                                         >  1:5361278/1‑101 (MQ=255)
         gCTGGGCGTGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTgg                                                                                        <  1:5709455/101‑1 (MQ=255)
            gggCGTGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGGTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCt                                                                                     <  1:3234017/101‑1 (MQ=255)
               cGTGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCgg                                                                                  <  2:4540793/101‑1 (MQ=255)
               cGTGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCgg                                                                                  <  2:3270305/101‑1 (MQ=255)
                 tGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGtt                                                                                >  2:4370954/1‑101 (MQ=255)
                 tGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGtt                                                                                <  1:220499/101‑1 (MQ=255)
                 tGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTCGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGtt                                                                                >  1:5015692/1‑101 (MQ=255)
                  gCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGttt                                                                               >  2:4973784/1‑101 (MQ=255)
                   cTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGtttt                                                                              <  1:2728728/101‑1 (MQ=255)
                    tGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTg                                                                             <  2:3450401/101‑1 (MQ=255)
                     gATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTgt                                                                            >  1:2567911/1‑100 (MQ=255)
                      aTCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTgtt                                                                           <  1:5600299/101‑3 (MQ=255)
                       tCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTg                                                                          <  2:5312260/101‑1 (MQ=255)
                       tCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTg                                                                          >  2:1973707/1‑101 (MQ=255)
                          tttCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTgaag                                                                       <  1:4418182/101‑1 (MQ=255)
                             cGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGaaa                                                                    <  1:5053140/101‑1 (MQ=255)
                             cGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGaaa                                                                    <  1:246696/101‑1 (MQ=255)
                              ggCCGACAGCCTGTCGGTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGATAc                                                                   <  1:3300157/101‑1 (MQ=255)
                              ggCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAAc                                                                   <  2:3875262/101‑1 (MQ=255)
                              ggCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAAc                                                                   >  2:4066447/1‑101 (MQ=255)
                              ggCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAAc                                                                   <  2:4119814/101‑1 (MQ=255)
                              ggCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAAc                                                                   >  2:4728312/1‑101 (MQ=255)
                              ggCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAAc                                                                   <  1:4062672/101‑1 (MQ=255)
                               gCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACg                                                                  <  2:2163553/101‑1 (MQ=255)
                                 cGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGc                                                                >  1:1822267/1‑101 (MQ=255)
                                  gACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGcc                                                               <  1:4095193/101‑1 (MQ=255)
                                  gACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGcc                                                               <  2:537148/101‑1 (MQ=255)
                                   aCAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGccc                                                              <  1:1022458/101‑1 (MQ=255)
                                     aGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTGGAGGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAAGGGCCCgg                                                            >  2:5723310/1‑101 (MQ=255)
                                     aGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCgg                                                            >  1:1054073/1‑101 (MQ=255)
                                       ccTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGc                                                          >  2:5114230/1‑101 (MQ=255)
                                        cTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCt                                                         >  2:5095575/1‑101 (MQ=255)
                                         tGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTg                                                        <  2:3500659/101‑1 (MQ=255)
                                         tGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTg                                                        >  1:4453274/1‑101 (MQ=255)
                                           tCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCg                                                      >  1:3477741/1‑101 (MQ=255)
                                           tCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCg                                                      <  2:1755187/101‑1 (MQ=255)
                                            cGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCgg                                                     <  1:4120964/101‑1 (MQ=255)
                                             gCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGa                                                    <  1:5095860/101‑1 (MQ=255)
                                             gCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGa                                                    <  1:1492939/101‑1 (MQ=255)
                                             gCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGa                                                    >  1:1621009/1‑101 (MQ=255)
                                               tGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATg                                                  >  1:5415154/1‑101 (MQ=255)
                                                ggggACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGc                                                 >  2:5633015/1‑101 (MQ=255)
                                                   gACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCt                                              >  1:300638/1‑101 (MQ=255)
                                                      ggCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGc                                           >  2:5060591/1‑101 (MQ=255)
                                                       gcgcCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCa                                          <  2:4283740/101‑1 (MQ=255)
                                                       gcaccgcTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCa                                          <  2:4876957/98‑1 (MQ=255)
                                                         gccgcTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGCTGCCCTTGCCTc                                        >  1:1774419/1‑101 (MQ=255)
                                                         gccgcTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATc                                        <  2:165585/101‑1 (MQ=255)
                                                          ccgcTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCa                                       <  2:3368665/101‑1 (MQ=255)
                                                           cgcTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCaa                                      <  2:2972243/101‑1 (MQ=255)
                                                            gcTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGTTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCaaa                                     <  1:3334485/101‑1 (MQ=255)
                                                            gcTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCaaa                                     >  2:1421938/1‑101 (MQ=255)
                                                             cTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAg                                    <  1:2044309/101‑1 (MQ=255)
                                                              tttGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGc                                   >  1:760428/1‑101 (MQ=255)
                                                                tGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCt                                 <  1:600522/101‑1 (MQ=255)
                                                                 gggCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCtg                                <  2:766122/101‑1 (MQ=255)
                                                                 gggCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCtg                                <  2:1193369/101‑1 (MQ=255)
                                                                  ggCCTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCtgt                               >  1:3169235/1‑101 (MQ=255)
                                                                    ccTATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTgtgt                             >  1:967285/1‑101 (MQ=255)
                                                                      tATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCt                           >  2:899761/1‑101 (MQ=255)
                                                                      tATGGGCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCt                           <  2:4594842/101‑1 (MQ=255)
                                                                          ggCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCAt                       >  1:4498390/1‑101 (MQ=255)
                                                                           gCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCAtt                      <  2:838019/101‑1 (MQ=255)
                                                                           gCTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCAtt                      >  2:5699499/1‑101 (MQ=255)
                                                                            cTGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTc                     <  2:2793435/101‑1 (MQ=255)
                                                                             tGACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCg                    <  2:5523415/101‑1 (MQ=255)
                                                                              gACGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGc                   >  1:3271091/1‑101 (MQ=255)
                                                                                cGGTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTg                 <  2:545346/101‑1 (MQ=255)
                                                                                 ggTGGCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGa                <  1:2372538/101‑1 (MQ=255)
                                                                                    ggCCTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCg             >  1:421160/1‑101 (MQ=255)
                                                                                    ggCCTCGATGCAGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCg             <  1:4584588/101‑1 (MQ=255)
                                                                                      ccTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCggg           <  2:2007469/101‑1 (MQ=255)
                                                                                       cTCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCgggg          >  1:3683237/1‑101 (MQ=255)
                                                                                        tCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGc         >  2:61135/1‑101 (MQ=255)
                                                                                        tCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGc         <  2:1053998/101‑1 (MQ=255)
                                                                                        tCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGc         >  1:5505364/1‑101 (MQ=255)
                                                                                        tCGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGc         >  1:4660468/1‑101 (MQ=255)
                                                                                         cGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCg        <  1:5218326/101‑1 (MQ=255)
                                                                                         cGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCg        >  2:4530372/1‑101 (MQ=255)
                                                                                         cGATGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCg        <  2:323527/101‑1 (MQ=255)
                                                                                          gATGCTGGTCTTTGCGCTGGCCTCGGGTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCgg       >  1:5536023/1‑101 (MQ=255)
                                                                                           aTGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCGGt      <  2:1858315/101‑1 (MQ=255)
                                                                                           aTGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCGGt      >  1:2185819/1‑101 (MQ=255)
                                                                                            tGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCGGTg     >  1:2763234/1‑101 (MQ=255)
                                                                                            tGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCGGTg     <  2:2452261/101‑1 (MQ=255)
                                                                                            tGCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCGGTg     >  1:1280236/1‑101 (MQ=255)
                                                                                             gCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCGGTGc    <  2:5015411/101‑1 (MQ=255)
                                                                                             gCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCGGTGc    >  1:809272/1‑101 (MQ=255)
                                                                                             gCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCGGTGc    <  2:3680214/101‑1 (MQ=255)
                                                                                             gCTGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCGGTGc    >  2:2721803/1‑101 (MQ=255)
                                                                                               tGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCGGTGCTg  >  1:3632079/1‑101 (MQ=255)
                                                                                               tGGTCTTTGCGCTGGCCTCGGTTTTGTTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCGGTGCTg  >  1:1675832/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GGTGGCGGTGCTGGGCGTGCTGATCGTTTCGGCCGACAGCCTGTCGCTGGGGACGGCGCCGCTTTGGGCCTATGGGCTGACGGTGGCCTCGATGCTGGGCTTTGCGCTGGCCTCGGTTTTGGTGAAGAAACGGCCCGGGCTGCGGATGCCCTTGCATCAAAGCCTGTGTCTGCATTCGCTGACCGGGGCGGTGCTG  >  NC_014034/445196‑445391

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG