BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	66,387	T→G	100%	S518S (TCT→TCG)	flhA →	flagellar biosynthesis protein FlhA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	66,387	0	T→G	100.0%	951.0	256	S518S (TCT→TCG)	flhA	flagellar biosynthesis protein FlhA
Reads supporting (aligned to +/- strand): new base (149/107): ref base (0/0): total (149/107)

Only 100 of 262 total aligned reads displayed.


GTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGACGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCTAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATCC  >  NC_014034/66289‑66484


gTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGaa                                                                                                 <  2:4831864/101‑1 (MQ=255)


 tGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAc                                                                                                >  2:879108/1‑101 (MQ=255)


 tGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAc                                                                                                <  2:367828/101‑1 (MQ=255)


  gATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAcc                                                                                               <  1:1019057/101‑1 (MQ=255)


   aTCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAccc                                                                                              >  2:2749765/1‑100 (MQ=255)


     ccGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAccgcc                                                                                            <  1:212032/101‑1 (MQ=255)


       ggCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACcgccgc                                                                                          >  1:4229002/1‑101 (MQ=255)


         cgcAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCt                                                                                        <  2:2987010/101‑1 (MQ=255)


            aaCTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCt                                                                                     <  2:1197041/101‑1 (MQ=255)


             aCTTCGGCTGACAGAGGACGCTGCGGCCGCTGCGCCGCCTGCTGGAGGAAGTGTTCAACCTTTCCGTTCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCtt                                                                                    <  2:5183675/101‑1 (MQ=255)


             aCTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCtt                                                                                    >  2:3820684/1‑101 (MQ=255)


             aCTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCtt                                                                                    >  1:5518947/1‑101 (MQ=255)


              cTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTg                                                                                   <  2:2504560/101‑1 (MQ=255)


               ttCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGa                                                                                  >  2:3478691/1‑101 (MQ=255)


               ttCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGa                                                                                  <  1:3705344/101‑1 (MQ=255)


                tCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGAc                                                                                 >  1:1399467/1‑101 (MQ=255)


                 cGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACg                                                                                <  1:1274150/101‑1 (MQ=255)


                  ggCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGa                                                                               >  1:1350034/1‑101 (MQ=255)


                  ggCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGa                                                                               >  1:2047714/1‑101 (MQ=255)


                    ccGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGc                                                                             <  2:178939/101‑1 (MQ=255)


                    ccGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGc                                                                             <  1:5093677/101‑1 (MQ=255)


                     cGTCTGATGAAGCTGCGGGCGCTGCGCCCCCTCGTGGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCt                                                                            <  2:4267858/101‑1 (MQ=255)


                       tCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGa                                                                          >  1:64130/1‑101 (MQ=255)


                       tCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGa                                                                          >  2:5274229/1‑101 (MQ=255)


                         tgatgaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATc                                                                        <  1:879184/101‑1 (MQ=255)


                         tgatgaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATc                                                                        >  1:2877186/1‑101 (MQ=255)


                             gaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCg                                                                    <  2:5118184/101‑1 (MQ=255)


                               cGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGAc                                                                  <  1:2749926/101‑1 (MQ=255)


                                ccTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACa                                                                 <  2:5290696/100‑1 (MQ=255)


                                 cTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACaa                                                                <  1:2566105/101‑1 (MQ=255)


                                  tgggggCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAg                                                               <  1:3925176/98‑1 (MQ=255)


                                  tGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAg                                                               <  2:4480064/101‑1 (MQ=255)


                                  tGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAg                                                               <  1:2360223/101‑1 (MQ=255)


                                   gCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCGGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAgg                                                              >  2:2958671/1‑101 (MQ=255)


                                   gCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAgg                                                              >  2:2651382/1‑101 (MQ=255)


                                   gCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAgg                                                              <  2:1301975/101‑1 (MQ=255)


                                    cGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGt                                                             >  2:1732314/1‑101 (MQ=255)


                                    cGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGt                                                             >  1:445208/1‑101 (MQ=255)


                                     gggCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGtt                                                            >  2:1774604/1‑101 (MQ=255)


                                     gggCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGtt                                                            <  1:2988231/101‑1 (MQ=255)


                                           tGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGCCGAGCTGATCCCCGACAAGGTTCCGGTc                                                      >  2:433977/1‑101 (MQ=255)


                                           tGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTc                                                      <  1:3846889/101‑1 (MQ=255)


                                           tGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTc                                                      >  1:3594573/1‑101 (MQ=255)


                                           tGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTc                                                      >  1:3239988/1‑101 (MQ=255)


                                           tGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTc                                                      >  2:792824/1‑101 (MQ=255)


                                              gccgccTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGAt                                                   <  2:2621870/101‑1 (MQ=255)


                                              gccgccTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGAt                                                   >  2:4022064/1‑101 (MQ=255)


                                              gccgccTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGAt                                                   >  1:5792847/1‑101 (MQ=255)


                                                  ccTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgc                                               >  1:1048440/1‑101 (MQ=255)


                                                     gCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgctgc                                            >  2:4770455/1‑101 (MQ=255)


                                                      cTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgctgct                                           <  1:3080428/101‑1 (MQ=255)


                                                        cggggAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTgg                                         >  1:4273238/4‑101 (MQ=255)


                                                        cGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTgg                                         >  1:4675318/1‑101 (MQ=255)


                                                        cGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTgg                                         <  1:1698501/101‑1 (MQ=255)


                                                         gAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGc                                        <  1:4286411/101‑1 (MQ=255)


                                                         gAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGc                                        >  1:52186/1‑101 (MQ=255)


                                                         gAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGc                                        >  1:207412/1‑101 (MQ=255)


                                                          aGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCg                                       >  2:2861431/1‑101 (MQ=255)


                                                          aGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCg                                       >  1:3806211/1‑101 (MQ=255)


                                                           ggAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCgg                                      >  1:4398289/1‑101 (MQ=255)


                                                            gaagtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCggg                                     >  2:2376460/4‑100 (MQ=255)


                                                             aagtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtg                                    >  2:5521073/3‑101 (MQ=255)


                                                              agtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgc                                   >  1:3047781/2‑101 (MQ=255)


                                                               gtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgct                                  >  2:4149347/1‑101 (MQ=255)


                                                               gtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgct                                  <  1:5425665/101‑1 (MQ=255)


                                                                tgtgCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgctg                                 >  1:3741712/1‑101 (MQ=255)


                                                                 gtgCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgctgc                                <  1:1867812/101‑1 (MQ=255)


                                                                  tgCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCg                               >  2:3905091/1‑101 (MQ=255)


                                                                    cAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTc                             >  1:2027405/1‑101 (MQ=255)


                                                                    cAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTc                             >  2:3744182/1‑101 (MQ=255)


                                                                    cAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTc                             >  2:3047527/1‑101 (MQ=255)


                                                                       ccTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgc                          <  2:2731059/101‑1 (MQ=255)


                                                                       ccTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgc                          <  2:1349946/101‑1 (MQ=255)


                                                                        cTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgct                         >  1:4789134/1‑101 (MQ=255)


                                                                        cTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgct                         >  2:1598674/1‑101 (MQ=255)


                                                                         tttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctg                        >  1:5250401/1‑101 (MQ=255)


                                                                          ttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgc                       >  2:731248/1‑101 (MQ=255)


                                                                          ttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgc                       >  1:2243584/1‑101 (MQ=255)


                                                                          ttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgc                       >  1:1201442/1‑101 (MQ=255)


                                                                           tCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgct                      >  2:3451751/1‑101 (MQ=255)


                                                                           tCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgct                      >  2:4332865/1‑101 (MQ=255)


                                                                            ccGACCCCGCCCGCGCCGAGTCGAACTGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgctg                     <  1:3478908/101‑1 (MQ=255)


                                                                             cGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTgg                    >  1:1543383/1‑101 (MQ=255)


                                                                              gATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGga                   <  1:2319018/101‑1 (MQ=255)


                                                                              gATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGCGCTGCGTCTGCTGCTGga                   >  2:2022419/1‑101 (MQ=255)


                                                                               aTCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaa                  <  1:4042584/101‑1 (MQ=255)


                                                                                 cccgcccgCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaaga                <  1:2951466/101‑1 (MQ=255)


                                                                                  ccgcccgcGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaagaa               <  1:792892/101‑1 (MQ=255)


                                                                                  ccgcccgcGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaagaa               >  2:2251914/1‑101 (MQ=255)


                                                                                   cgcccgcGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAAc              <  1:185799/101‑1 (MQ=255)


                                                                                    gcccgcGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACg             >  2:1124492/1‑101 (MQ=255)


                                                                                       cgcgCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACgggg          >  2:1020597/1‑100 (MQ=255)


                                                                                       cgcgCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACgggg          >  2:3640372/1‑100 (MQ=255)


                                                                                        gcgcCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGgtg         >  2:4601413/1‑101 (MQ=255)


                                                                                         cgcCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGgtgt        <  2:2147124/101‑1 (MQ=255)


                                                                                          gcCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTc       >  2:4238300/1‑101 (MQ=255)


                                                                                           ccGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGGTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCg      <  1:4867721/101‑1 (MQ=255)


                                                                                             gAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGAt    >  2:4922769/1‑101 (MQ=255)


                                                                                              aGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATc   >  2:5214469/1‑101 (MQ=255)


                                                                                               gTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATcc  <  2:2909503/101‑1 (MQ=255)


GTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGACGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCTAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATCC  >  NC_014034/66289‑66484

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG