Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,815 G→C 8.3% R1598G (CGG→GGC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,8150G→C 8.3% 39.5 ‑1.1 ‑0.4230R1598G (CGG→GGG) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (12/7):  ref base (106/105):  total (118/112)
Fisher's exact test strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.14e-01

Only 100 of 246 total aligned reads displayed.

ATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGCGCCGCC  >  NC_014034/678715‑678915
                                                                                                    |                                                                                                    
aTCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCg                                                                                                      >  1:1601275/1‑101 (MQ=255)
 tCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCgg                                                                                                     >  2:4350879/1‑100 (MQ=255)
  ccccGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTg                                                                                                    <  2:133631/101‑1 (MQ=255)
  ccccGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTg                                                                                                    >  1:3706346/1‑101 (MQ=255)
  ccccGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTg                                                                                                    >  1:191112/1‑101 (MQ=255)
     cGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccg                                                                                                 <  2:2914665/101‑1 (MQ=255)
      gCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccgc                                                                                                >  1:109387/1‑101 (MQ=255)
       ccATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccgcc                                                                                               >  2:1949707/1‑101 (MQ=255)
        cATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCt                                                                                              >  2:4179909/1‑101 (MQ=255)
        cATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCt                                                                                              <  2:592956/101‑1 (MQ=255)
         aTGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTg                                                                                             >  1:3140602/1‑101 (MQ=255)
           ggCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgcg                                                                                           >  2:2715918/1‑101 (MQ=255)
              cGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCg                                                                                        >  1:2609435/1‑101 (MQ=255)
               gaggCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGt                                                                                       <  1:2419085/101‑1 (MQ=255)
                 gaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTgg                                                                                     >  1:301650/1‑101 (MQ=255)
                  aCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGt                                                                                    >  2:3684590/1‑101 (MQ=255)
                  aCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGt                                                                                    >  1:3187563/1‑101 (MQ=255)
                   ccGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTc                                                                                   <  1:714316/101‑1 (MQ=255)
                    cGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGGCGCCTGCGCCGTGGTCa                                                                                  >  2:5197856/1‑101 (MQ=255)
                     gAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAg                                                                                 >  2:4585323/1‑101 (MQ=255)
                      aaaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgc                                                                                <  2:5030534/101‑1 (MQ=255)
                       aaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgcg                                                                               <  1:382841/101‑1 (MQ=255)
                       aaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgcg                                                                               >  2:5405928/1‑101 (MQ=255)
                          ccgcaccagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCa                                                                            >  1:2120853/10‑101 (MQ=2)
                          cTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCa                                                                            >  2:3255479/1‑101 (MQ=255)
                           tGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGcccc                                                                           >  1:1361434/1‑99 (MQ=255)
                           cgcaccagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                                           >  1:2233743/9‑101 (MQ=2)
                            gggCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACg                                                                          <  2:5094911/101‑1 (MQ=255)
                            gcaccagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACg                                                                          >  1:5513676/8‑101 (MQ=2)
                            gcaccagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACg                                                                          >  1:1257668/8‑101 (MQ=2)
                             caccagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGa                                                                         >  1:3549038/7‑101 (MQ=2)
                              gCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGAt                                                                        <  2:3776513/101‑1 (MQ=255)
                              gCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGAt                                                                        <  1:3961148/101‑1 (MQ=255)
                               cGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGacg                                                                       >  2:557567/1‑99 (MQ=255)
                               cGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATg                                                                       >  1:4754360/1‑101 (MQ=255)
                                gATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGt                                                                      <  2:3556655/101‑1 (MQ=255)
                                cagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGt                                                                      >  2:2604188/4‑101 (MQ=2)
                                 atatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTc                                                                     >  2:4818359/1‑101 (MQ=255)
                                 atatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTc                                                                     >  1:4004075/1‑101 (MQ=255)
                                 atatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTc                                                                     >  1:2872800/1‑101 (MQ=255)
                                 agatCGGCGGTTTCCAGCGCCGCAACCTGCGTGTTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                                                     <  1:4934867/99‑1 (MQ=2)
                                 agatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                                                     >  1:4472149/3‑101 (MQ=2)
                                   atCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGCCGTCAGCGCCACGATGTCAt                                                                   <  2:4129182/101‑1 (MQ=2)
                                    tCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCAtt                                                                  <  2:4853/101‑1 (MQ=255)
                                     ccGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTc                                                                 >  2:3235902/1‑101 (MQ=255)
                                     ccGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTc                                                                 <  1:1760110/101‑1 (MQ=255)
                                      cgcgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCg                                                                <  2:4030/101‑1 (MQ=255)
                                        cgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTg                                                              <  1:4180159/101‑1 (MQ=255)
                                         ggTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGc                                                             <  1:5615790/101‑1 (MQ=255)
                                           tttCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGccc                                                           >  1:3404085/1‑101 (MQ=255)
                                           tttCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGccc                                                           >  2:1043965/1‑101 (MQ=255)
                                           tttCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGccc                                                           >  1:5785543/1‑101 (MQ=255)
                                            ttCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCa                                                          <  2:4400097/101‑1 (MQ=255)
                                            ttCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCa                                                          <  2:1119455/101‑1 (MQ=255)
                                             tCCAGCGCCGCGATCTGAGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAg                                                         >  2:3879472/1‑101 (MQ=255)
                                                aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGccc                                                      <  1:2041847/101‑1 (MQ=255)
                                                aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGccc                                                      >  1:1708438/1‑101 (MQ=255)
                                                  cgccgcGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGCTCAGCGCCACGATGTCATTCGTGCCCAGCCCCt                                                    >  1:4980961/1‑101 (MQ=255)
                                                     cgcgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCa                                                 <  2:2444853/101‑1 (MQ=255)
                                                        gATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgcg                                              <  2:3410977/101‑1 (MQ=255)
                                                         aTCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgcgc                                             >  2:1627646/1‑101 (MQ=255)
                                                          tCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgcc                                            <  2:1206041/101‑1 (MQ=255)
                                                          tCTGCGCCGTGCTCAGCGCCGCGATCTGCGTAGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgcc                                            >  1:5238047/1‑101 (MQ=255)
                                                            tGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgccgc                                          >  2:3902444/1‑101 (MQ=255)
                                                             gcgcCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCcgcg                                         >  2:1265167/1‑101 (MQ=255)
                                                              cgcCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCgg                                        >  2:2379524/1‑101 (MQ=255)
                                                               gcCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGt                                       >  2:672955/1‑101 (MQ=255)
                                                                   tGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTc                                   <  1:4818745/101‑1 (MQ=255)
                                                                   tGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAACGCCGCGGTGCTc                                   >  2:1259526/1‑101 (MQ=255)
                                                                    gCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCa                                  <  2:191092/101‑1 (MQ=255)
                                                                     cTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAg                                 <  2:3135554/101‑1 (MQ=255)
                                                                     cTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAg                                 >  1:1457541/1‑101 (MQ=255)
                                                                       cAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGCCATTCGTGCCCAGCCCCTTAAGCACCGCGGTGCTCAacg                               >  2:3374585/1‑101 (MQ=37)
                                                                          cgccgcGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAgcgcgc                            >  2:200363/1‑101 (MQ=255)
                                                                              gcgATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGAt                        <  1:328374/101‑1 (MQ=255)
                                                                              gcgATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGAt                        >  1:3933386/1‑101 (MQ=255)
                                                                               cgATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCGCTTCAGCGCCGCGGTGCTCAGCGCGCCGATc                       <  1:3250000/101‑1 (MQ=255)
                                                                               cgATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATc                       <  2:5353760/101‑1 (MQ=255)
                                                                                gATCTGCGTCGCGCTCAGCCGTGCCGCCTGGGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCg                      <  1:52907/101‑1 (MQ=255)
                                                                                gATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCg                      <  1:1263905/101‑1 (MQ=255)
                                                                                gATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCg                      >  1:1461579/1‑101 (MQ=255)
                                                                                    tGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGcccc                  >  1:4853398/1‑101 (MQ=255)
                                                                                       gTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGCTCGCCCCGGt               >  2:2697133/1‑101 (MQ=255)
                                                                                       gTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCggc               >  2:1697382/1‑100 (MQ=255)
                                                                                       gTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGt               <  1:4398251/101‑1 (MQ=255)
                                                                                        tCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTc              <  2:3852885/101‑1 (MQ=255)
                                                                                           cgcTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGAc           <  1:1414816/101‑1 (MQ=255)
                                                                                            gcTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACa          >  1:4152112/1‑101 (MQ=255)
                                                                                            gcTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACa          >  2:5214850/1‑101 (MQ=255)
                                                                                            ccTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACa          <  2:4317556/100‑1 (MQ=255)
                                                                                             cTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAg         <  2:5294885/101‑1 (MQ=255)
                                                                                              tCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAgc        >  2:5457340/1‑101 (MQ=255)
                                                                                               cAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAgcg       >  2:5361443/1‑101 (MQ=255)
                                                                                                aGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAgcgc      >  2:5137512/1‑101 (MQ=255)
                                                                                                aGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAgcgc      >  1:5388552/1‑101 (MQ=255)
                                                                                                 gCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGcgcc     <  2:2581907/101‑1 (MQ=255)
                                                                                                 gCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGcgcc     >  2:4556560/1‑101 (MQ=255)
                                                                                                  ccGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGcgccg    >  1:5072880/1‑101 (MQ=255)
                                                                                                    gTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGCgccgcc  >  2:1537833/1‑101 (MQ=255)
                                                                                                    gTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGCgccgcc  <  1:4351145/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
ATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGCGCCGCC  >  NC_014034/678715‑678915

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG