Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 682,521 G→C 6.6% A362A (GCC→GCG RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034682,5210G→C 6.6% 26.9 ‑2.2 ‑0.0228A362A (GCC→GCGRCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (4/11):  ref base (107/106):  total (111/117)
Fisher's exact test strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.18e-01

Only 100 of 246 total aligned reads displayed.

TCTGCGCGGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGG  >  NC_014034/682421‑682621
                                                                                                    |                                                                                                     
 cTGCGCGGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTggg                                                                                                      <  1:4526092/101‑1 (MQ=255)
    cgcgGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCg                                                                                                   <  1:2977204/101‑1 (MQ=255)
    cgcgGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCg                                                                                                   >  2:475274/1‑101 (MQ=255)
     gcgGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGt                                                                                                  <  2:4540660/101‑1 (MQ=255)
      cgGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTg                                                                                                 >  2:691346/1‑101 (MQ=255)
      cgGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTg                                                                                                 <  1:2427474/101‑1 (MQ=255)
       ggTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGc                                                                                                >  2:4114895/1‑101 (MQ=255)
       ggTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGc                                                                                                <  1:4698265/101‑1 (MQ=255)
        gTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCt                                                                                               >  2:3178974/1‑101 (MQ=255)
           gACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAg                                                                                            >  2:4380916/1‑101 (MQ=255)
           gACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAg                                                                                            >  1:3243138/1‑101 (MQ=255)
             cAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCAGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAgcg                                                                                          <  1:3446146/101‑1 (MQ=255)
              aaGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAgcgc                                                                                         >  2:1617807/1‑101 (MQ=255)
              aaGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCACCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAgcgc                                                                                         >  2:3223613/1‑101 (MQ=255)
                ggCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGcgccg                                                                                       <  1:4789755/101‑1 (MQ=255)
                  cGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCgccgcc                                                                                     >  1:5250777/1‑101 (MQ=255)
                  cGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCgccgcc                                                                                     <  2:3965279/101‑1 (MQ=255)
                  cGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCgccgcc                                                                                     >  1:553991/1‑101 (MQ=255)
                  cGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCACAACCCCCAGCTGGGCCGTGCTCAGCgccgcc                                                                                     <  1:5160891/101‑1 (MQ=255)
                   gTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCa                                                                                    >  2:3582179/1‑101 (MQ=255)
                   gTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCACCAGCTGGGCCGTGCTCAGCGACGCCa                                                                                    >  2:1923578/1‑101 (MQ=255)
                    tCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCAt                                                                                   >  2:143696/1‑101 (MQ=255)
                     cGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATc                                                                                  >  2:1513787/1‑101 (MQ=255)
                      gATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCt                                                                                 <  1:3737025/101‑1 (MQ=255)
                      gATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCt                                                                                 <  2:4846258/101‑1 (MQ=255)
                      gATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCt                                                                                 <  1:1243694/101‑1 (MQ=255)
                       aTCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTg                                                                                <  1:5524186/101‑1 (MQ=255)
                            cgcCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGacc                                                                           >  2:5296773/1‑101 (MQ=255)
                              ccGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCg                                                                         >  1:2029682/1‑101 (MQ=255)
                                gAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGt                                                                       <  2:4648327/101‑1 (MQ=255)
                                    tCAGCGCCTCGGCGGCCTCCTCGGACAGCTCCGCGATCTGCCGCGTGCCCAGATCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgc                                                                   <  2:3500258/101‑1 (MQ=255)
                                    tCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGGCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgc                                                                   >  2:5006535/1‑101 (MQ=255)
                                     cAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgcg                                                                  <  1:4395835/101‑1 (MQ=255)
                                        cgcCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGcgccg                                                               >  1:4168389/1‑101 (MQ=255)
                                        cgcCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGcgccg                                                               >  2:1280917/1‑101 (MQ=255)
                                        cgcCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGcgccg                                                               >  1:5042540/1‑101 (MQ=255)
                                          ccTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCgccgcc                                                             >  2:4719623/1‑101 (MQ=255)
                                           cTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCa                                                            <  1:5454770/101‑1 (MQ=255)
                                             cggcggCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGt                                                          >  2:1789431/1‑101 (MQ=255)
                                             cggcggCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGt                                                          >  1:4763816/1‑101 (MQ=255)
                                              ggcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGtt                                                         >  2:3038716/1‑101 (MQ=255)
                                               gcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTc                                                        <  1:691403/101‑1 (MQ=255)
                                               gcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTc                                                        <  2:85829/101‑1 (MQ=255)
                                               gcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTc                                                        <  1:1826212/101‑1 (MQ=255)
                                               gcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTc                                                        <  1:1281000/101‑1 (MQ=255)
                                                cggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCg                                                       <  1:2671827/101‑1 (MQ=255)
                                                cgccctcctCGGACAGCGCCGCGATCTGCCGCGTGCCGAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCg                                                       <  2:412229/98‑1 (MQ=255)
                                                 ggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGa                                                      >  1:5328636/1‑101 (MQ=255)
                                                  gcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGAt                                                     <  2:1702543/101‑1 (MQ=255)
                                                     tcctcGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.t                                                 >  2:2184891/1‑101 (MQ=255)
                                                       ctcGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGc                                               <  2:1542128/101‑1 (MQ=255)
                                                         cGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTc                                             <  2:2096377/101‑1 (MQ=255)
                                                         cGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTc                                             >  1:5677873/1‑101 (MQ=255)
                                                          ggACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCa                                            <  1:2795677/101‑1 (MQ=255)
                                                              aGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGccc                                        >  2:4501536/1‑101 (MQ=255)
                                                              aGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGACAGTTCGATCG.TGCTCAGccc                                        <  1:5259214/101‑1 (MQ=255)
                                                               gcgcCGCGATCTGCCGCGTGCCCAGAGCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCg                                       <  2:5387384/101‑1 (MQ=255)
                                                               gcgcCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCg                                       <  2:3853434/101‑1 (MQ=255)
                                                                     cgATCTGCCGCCTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATg                                 >  2:2436878/1‑101 (MQ=255)
                                                                        tCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTcc                              <  1:803747/101‑1 (MQ=255)
                                                                        tCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTcc                              >  1:1678951/1‑101 (MQ=255)
                                                                         cTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCt                             <  1:1304855/101‑1 (MQ=255)
                                                                         cTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTCCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCt                             <  1:4927770/101‑1 (MQ=255)
                                                                          tGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTc                            <  2:678424/101‑1 (MQ=255)
                                                                           gCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCg                           <  1:2146619/101‑1 (MQ=255)
                                                                              gcgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTc                        >  2:4969593/1‑101 (MQ=255)
                                                                               cgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCg                       <  1:1424556/101‑1 (MQ=255)
                                                                               cgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCg                       <  1:979410/101‑1 (MQ=255)
                                                                               cgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCg                       <  1:2289403/101‑1 (MQ=255)
                                                                                gTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGa                      >  2:1834787/1‑101 (MQ=255)
                                                                                 tGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGaa                     <  2:645077/101‑1 (MQ=255)
                                                                                    ccAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGc                  >  1:3662654/1‑101 (MQ=255)
                                                                                      aGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGccc                >  1:3007890/1‑101 (MQ=255)
                                                                                       tcgccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGcccc               <  2:579044/95‑1 (MQ=37)
                                                                                       gAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGcccc               <  2:5677664/101‑1 (MQ=255)
                                                                                       gAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGcccc               >  1:107857/1‑101 (MQ=255)
                                                                                        cgccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCGGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCt              <  2:2830559/96‑1 (MQ=25)
                                                                                         tcccccGGCTGGGCCGTGCTCAGCTGCGCCATCTGTGCCCGGGTCAGGGCCGCCAGTTCGATCG.GGCGCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCtt             <  2:4255457/100‑1 (MQ=255)
                                                                                         gccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt             >  2:2353614/5‑101 (MQ=37)
                                                                                         gccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt             <  1:3964314/97‑1 (MQ=37)
                                                                                         aCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCtt             <  2:446282/101‑1 (MQ=255)
                                                                                         aCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCtt             <  2:4983283/101‑1 (MQ=255)
                                                                                          ccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCttg            <  2:4270997/98‑2 (MQ=37)
                                                                                          cccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCGATGTCCTCGGTCGAAAGCCCCATc            >  2:1480238/1‑101 (MQ=255)
                                                                                          cccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTc            <  1:5392958/101‑1 (MQ=255)
                                                                                          cccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTc            <  1:2732361/101‑1 (MQ=255)
                                                                                           cgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCttga           <  2:3074647/99‑3 (MQ=37)
                                                                                            gccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAg          <  1:5768491/100‑1 (MQ=25)
                                                                                            cccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAg          <  2:2596701/101‑1 (MQ=255)
                                                                                            cccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAg          >  1:2839691/1‑101 (MQ=255)
                                                                                             ccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAgc         <  2:2471052/101‑1 (MQ=25)
                                                                                              cAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAgcg        >  2:5601836/1‑101 (MQ=25)
                                                                                               aGCTGGGCCGTGCTCAGCGCCGCCATCTGCGGCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgcgc       >  1:5125633/1‑101 (MQ=255)
                                                                                               aGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgcgc       <  1:1513880/101‑1 (MQ=255)
                                                                                               aGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgcgc       >  2:1036771/1‑101 (MQ=255)
                                                                                                  tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc    <  1:4115144/101‑1 (MQ=255)
                                                                                                  tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc    >  1:435044/1‑101 (MQ=255)
                                                                                                  tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc    >  2:803564/1‑101 (MQ=255)
                                                                                                   gggCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggcg   >  2:137751/1‑101 (MQ=255)
                                                                                                    ggCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGcggcgg  <  2:3386722/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
TCTGCGCGGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGG  >  NC_014034/682421‑682621

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG