Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 74,095 A→C 100% F196L (TTT→TTG trmE ← tRNA modification GTPase TrmE

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_01403474,0950A→C100.0% 1168.8 303F196L (TTT→TTGtrmEtRNA modification GTPase TrmE
Reads supporting (aligned to +/- strand):  new base (145/158):  ref base (0/0):  total (145/158)

Only 100 of 308 total aligned reads displayed.

AGAAGCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGAAAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATCAGCG  >  NC_014034/73997‑74192
                                                                                                  |                                                                                                 
aGAAGCGTCGATTTCCCGGCATTCGGCCGCCGGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCCCCGAAATCCGGCCGATTTCCGCCGacaa                                                                                                 >  1:3233559/1‑101 (MQ=255)
  aaGCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACt                                                                                               <  2:5052945/101‑1 (MQ=255)
    gCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTgc                                                                                             <  2:2082237/101‑1 (MQ=255)
      gTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTgcgc                                                                                           <  2:4307435/101‑1 (MQ=255)
        cGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGcgccg                                                                                         >  1:1426879/1‑101 (MQ=255)
        cGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGcgccg                                                                                         <  1:5160822/101‑1 (MQ=255)
          aTTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCa                                                                                       >  1:3765902/1‑101 (MQ=255)
             tCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCGCGCACCCGTTCGGTCACCGGAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAAcc                                                                                    >  1:4944076/1‑101 (MQ=255)
              cccGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTACCCGCCCCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCt                                                                                   <  2:5729769/101‑1 (MQ=255)
               ccGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTc                                                                                  <  1:4147295/101‑1 (MQ=255)
               ccGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTc                                                                                  <  2:3200232/101‑1 (MQ=255)
                 ggCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCgg                                                                                >  1:2379516/1‑101 (MQ=255)
                 ggCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCgg                                                                                <  1:3574236/101‑1 (MQ=255)
                   cATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAc                                                                              <  1:4547859/101‑1 (MQ=255)
                      tCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGCcagc                                                                           >  2:715203/1‑101 (MQ=255)
                      tCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAcagc                                                                           <  1:2223307/101‑1 (MQ=255)
                      tCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAcagc                                                                           <  1:1259478/101‑1 (MQ=255)
                        ggCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAcagcag                                                                         >  1:3054231/1‑101 (MQ=255)
                         gccgccCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACagcagc                                                                        >  1:5570365/1‑101 (MQ=255)
                           cgccCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAgcgc                                                                      >  1:2948310/1‑101 (MQ=255)
                            gccCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCa                                                                     <  2:3376338/101‑1 (MQ=255)
                              ccGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCaaa                                                                   <  2:1479476/101‑1 (MQ=255)
                              ccGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCaaa                                                                   <  2:2345257/101‑1 (MQ=255)
                               cgacgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCaaaa                                                                  <  2:851791/101‑1 (MQ=255)
                               cgacgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCaaaa                                                                  <  2:4479522/101‑1 (MQ=255)
                               cgacgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCaaaa                                                                  <  1:2453451/101‑1 (MQ=255)
                                gacgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAg                                                                 <  2:5178673/101‑1 (MQ=255)
                                gacgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAg                                                                 >  2:3967637/1‑101 (MQ=255)
                                 acgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGc                                                                <  1:1502954/101‑1 (MQ=255)
                                  cgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGcc                                                               >  1:4435370/1‑101 (MQ=255)
                                  cgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGcc                                                               >  2:1798632/1‑101 (MQ=255)
                                    atggCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCta                                                             >  1:3308874/4‑100 (MQ=255)
                                    aTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTc                                                             >  2:1374635/1‑101 (MQ=255)
                                    aTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTc                                                             >  2:2591400/1‑101 (MQ=255)
                                     tCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTcc                                                            >  2:3087323/1‑101 (MQ=255)
                                     acagaaCTTCAAAACCGTCCCGCACCCGTTCGGGCACCCAAATCCGGCCGATTCCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTcc                                                            <  2:2626534/97‑1 (MQ=255)
                                      cGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCg                                                           >  1:3153800/1‑101 (MQ=255)
                                       gCAACTTCAAAGCCTTCCCGCCCCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCgg                                                          <  2:5388087/101‑1 (MQ=255)
                                       gCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCgg                                                          >  1:5410647/1‑101 (MQ=255)
                                           cTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTc                                                      <  2:3694268/101‑1 (MQ=255)
                                           cTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTc                                                      <  1:1092854/101‑1 (MQ=255)
                                           cTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTc                                                      <  1:1570558/101‑1 (MQ=255)
                                           cTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTc                                                      <  1:3505794/101‑1 (MQ=255)
                                            ttCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTca                                                     <  1:586597/101‑1 (MQ=255)
                                             tCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAGCCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTcac                                                    <  1:4968415/101‑1 (MQ=255)
                                              cAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTcaca                                                   >  2:4152876/1‑101 (MQ=255)
                                               aaaGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACAt                                                  >  2:1714507/1‑101 (MQ=255)
                                                aaGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATc                                                 >  1:2601754/1‑101 (MQ=255)
                                                aaGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATc                                                 >  1:2762039/1‑101 (MQ=255)
                                                  gCCTCCCCGCTCCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCaa                                               <  2:3492474/101‑1 (MQ=255)
                                                   ccTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAAc                                              >  2:5740534/1‑101 (MQ=255)
                                                   ccTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAAc                                              <  1:4632669/101‑1 (MQ=255)
                                                   ccTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAAc                                              >  1:4757022/1‑101 (MQ=255)
                                                    cTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAAcc                                             <  2:4659739/101‑1 (MQ=255)
                                                    cTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAAcc                                             <  1:2088860/101‑1 (MQ=255)
                                                    cTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAAcc                                             >  1:3641548/1‑101 (MQ=255)
                                                    cTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAAcc                                             <  1:76348/101‑1 (MQ=255)
                                                     ttCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCg                                            <  1:4329678/101‑1 (MQ=255)
                                                     ttCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCg                                            <  1:5450782/101‑1 (MQ=255)
                                                      tCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCgg                                           >  2:638476/1‑101 (MQ=255)
                                                       cccGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGc                                          >  2:3613661/1‑101 (MQ=255)
                                                          gCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAgg                                       >  1:2353496/1‑101 (MQ=255)
                                                            aCCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTc                                     <  1:5601794/101‑1 (MQ=255)
                                                                gTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCa                                 >  1:5353363/1‑101 (MQ=255)
                                                                gTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCa                                 >  1:1338985/1‑101 (MQ=255)
                                                                gTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACANCAACCGGCAGGTCTTCa                                 >  2:542122/1‑101 (MQ=255)
                                                                 ttCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCAt                                <  1:4362676/101‑1 (MQ=255)
                                                                  tCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATc                               <  1:292522/101‑1 (MQ=255)
                                                                   cGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCg                              <  1:1655898/101‑1 (MQ=255)
                                                                   cGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCg                              <  2:5493099/101‑1 (MQ=255)
                                                                   cGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCg                              >  1:1853824/1‑101 (MQ=255)
                                                                     gTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGc                            >  1:2869490/1‑101 (MQ=255)
                                                                     gTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGc                            <  2:222187/101‑1 (MQ=255)
                                                                      tCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCg                           >  2:1261716/1‑101 (MQ=255)
                                                                       cACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGa                          >  1:2503826/1‑101 (MQ=255)
                                                                        aCCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGaa                         >  1:3460194/1‑101 (MQ=255)
                                                                          cGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAAt                       <  2:3818349/101‑1 (MQ=255)
                                                                           gAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATc                      >  2:1625431/1‑101 (MQ=255)
                                                                           gAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATc                      <  2:1952538/101‑1 (MQ=255)
                                                                           gAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATc                      >  1:1205139/1‑101 (MQ=255)
                                                                           gAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATc                      <  2:5225339/101‑1 (MQ=255)
                                                                             aaTCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAGATCgg                    >  2:5593164/1‑100 (MQ=255)
                                                                             aaTCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGa                    >  1:1490089/1‑101 (MQ=255)
                                                                               tCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATg                  <  2:4384678/101‑1 (MQ=255)
                                                                                  ggCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAg               <  2:1500677/101‑1 (MQ=255)
                                                                                  ggCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAg               >  2:1555504/1‑101 (MQ=255)
                                                                                   gCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAgg              >  1:1797838/1‑101 (MQ=255)
                                                                                   gCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAgg              >  2:4857282/1‑101 (MQ=255)
                                                                                    ccGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGc             >  2:2881780/1‑101 (MQ=255)
                                                                                      gATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCt           <  2:2457029/101‑1 (MQ=255)
                                                                                      gATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCt           >  2:3295236/1‑101 (MQ=255)
                                                                                       aTTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCta          >  1:2628313/1‑100 (MQ=255)
                                                                                       aTTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTc          <  1:1714606/101‑1 (MQ=255)
                                                                                          tCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCAt       <  2:1031806/101‑1 (MQ=255)
                                                                                          tCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCAt       <  1:3013485/101‑1 (MQ=255)
                                                                                          tCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCAt       <  2:1213560/101‑1 (MQ=255)
                                                                                          tCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCAt       <  2:2761878/101‑1 (MQ=255)
                                                                                            cgccgACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATCa     <  1:163904/101‑1 (MQ=255)
                                                                                             gccgACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATCAg    <  2:4756741/101‑1 (MQ=255)
                                                                                             gccgACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATCAg    <  2:442969/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AGAAGCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGAAAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATCAGCG  >  NC_014034/73997‑74192

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG