Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	974,361	C→G	100%	intergenic (+241/+18)	RCAP_rcc00884 → / ← RCAP_rcc00885	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	974,361	0	C→G	100.0%	242.4	63	intergenic (+241/+18)	RCAP_rcc00884/RCAP_rcc00885	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (34/29):  ref base (0/0):  total (34/29)

CGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.GGGGT..GTGCG.GAAGCCCCCGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCCGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGC  >  NC_014034/974268‑974456                                                                                                  |                                                                                                cgcAGCGGCTTGTTTTCCCCGTGCGACAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCcggcc                                                                                              <  2:3714322/101‑1 (MQ=2)                tCACCTTGCGACAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGGGGCGCGGCGGCGGGCCAGGCGGAAAACCCCCGCCGCAGCGGcgcgggct                                                                               >  2:1952969/1‑94 (MQ=0)                                              ggggcggccccccgGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGcgcgcg                                                 <  2:293549/92‑1 (MQ=37)                                                  cggaGCCCCCGGCGGGGCGCCTCGGCGGCAGCCGGGGGGGAAAAACCCCGCCGCAACGGCCGGGTCTCCGCCGCACAGGAGAAGGGTCGAGcgcgcgcgca                                             >  2:3120487/4‑97 (MQ=255)                                                   ccccgcgggggcggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCAc                                            <  1:4022456/92‑1 (MQ=39)                                                    gaGCCCCCGGCGGGTCGCCTCGGCGCCCGCCGAGGGGAAAAAAACCGGCCGCCACGGCCGGGTCTCAGCCGAACAGGAGCAGGGGCCAGCGCGGGCTCAcc                                           >  2:617724/2‑101 (MQ=16)                                                      gCCCCGGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCa                                         <  1:3481282/101‑1 (MQ=255)                                                       ccccgGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCag                                        <  2:2981150/101‑1 (MQ=255)                                                              cggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtca                                 >  2:2469584/1‑101 (MQ=255)                                                              cggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtca                                 <  2:499178/101‑1 (MQ=255)                                                              cggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtca                                 >  1:4700099/1‑101 (MQ=255)                                                               gggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcat                                <  2:1021136/101‑1 (MQ=255)                                                               gggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGGTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCCGAGGGgcat                                >  1:1117107/1‑101 (MQ=39)                                                                ggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcatc                               <  1:1562013/101‑1 (MQ=255)                                                                ggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcatc                               <  2:5735496/101‑1 (MQ=255)                                                                  gcgctgcGGCGACAGCCGGGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAg                             <  2:5737399/95‑1 (MQ=255)                                                                   cGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAgc                            >  1:1824657/1‑101 (MQ=255)                                                                   cGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAgc                            >  2:3993631/1‑101 (MQ=255)                                                                      cTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCa                         <  2:1437176/101‑1 (MQ=255)                                                                      cTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCa                         <  1:1550682/101‑1 (MQ=255)                                                                      cTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGGCATCAGCGCa                         >  2:2615232/1‑101 (MQ=255)                                                                        cGGCGACAGCCGAGGCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATa                       <  2:1349323/101‑1 (MQ=255)                                                                        cGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATa                       >  2:4566168/1‑101 (MQ=255)                                                                        cGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATa                       <  1:5573426/101‑1 (MQ=255)                                                                         ggCGCCAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAg                      <  2:4839134/101‑1 (MQ=255)                                                                         ggCGACAGCGGAGGTGAAATACCCGGGCCGCAACGGCCGGTTCTCAGCCGAACAGGAGCAGGTTCGAGCGCGCGATCCCCCAGAGGGTCATCAGCGCATAg                      <  2:2620290/101‑1 (MQ=255)                                                                          gggACGGCCGAGGCGAAATGCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAgg                     <  1:20678/99‑1 (MQ=255)                                                                          gCGACAGCCGAGGCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAgg                     <  1:1182742/101‑1 (MQ=255)                                                                            gACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGcc                   <  1:4102953/101‑1 (MQ=255)                                                                            gACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGcc                   <  2:783643/101‑1 (MQ=255)                                                                             aCAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGATCGCGCGATCACCCAGAGGGTCATCAGCCCATAGGCCg                  >  1:180263/1‑101 (MQ=255)                                                                             aCAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCg                  >  1:3147121/1‑101 (MQ=255)                                                                             aCAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCg                  >  1:2996755/1‑101 (MQ=255)                                                                              cAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGc                 >  2:2933060/1‑101 (MQ=255)                                                                               aGCCGAGGCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCa                <  2:3603010/101‑1 (MQ=255)                                                                               aGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCa                <  2:2882457/101‑1 (MQ=255)                                                                               aGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCa                >  1:1094015/1‑101 (MQ=255)                                                                               aGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCa                >  1:2971613/1‑101 (MQ=255)                                                                               aGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCa                >  1:4293375/1‑101 (MQ=255)                                                                                gCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCaa               <  2:718845/101‑1 (MQ=255)                                                                                gCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCaa               >  2:5131555/1‑101 (MQ=255)                                                                                gCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCaa               >  1:3347703/1‑101 (MQ=255)                                                                                gCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCaa               >  1:5619541/1‑101 (MQ=255)                                                                                 ccGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCaaa              <  2:3035136/101‑1 (MQ=255)                                                                                 ccGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCaaa              >  2:2474900/1‑101 (MQ=255)                                                                                  cGAGGCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAc             <  1:5790206/101‑1 (MQ=255)                                                                                  cGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAc             >  1:1552906/1‑101 (MQ=255)                                                                                  cGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAc             >  2:1250689/1‑101 (MQ=255)                                                                                  cGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAc             <  1:796231/101‑1 (MQ=255)                                                                                  cGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAc             >  1:66134/1‑101 (MQ=255)                                                                                  cGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGGCATCAGCGCATAGGCCGCAAAc             >  2:4194896/1‑101 (MQ=255)                                                                                   gAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcc            <  1:1618973/101‑1 (MQ=255)                                                                                   gAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcc            >  1:1500989/1‑101 (MQ=255)                                                                                   gAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcc            >  2:3411244/1‑101 (MQ=255)                                                                                    aGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAccc           >  2:1203185/1‑101 (MQ=255)                                                                                     ggCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcccc          <  1:617782/101‑1 (MQ=255)                                                                                     ggCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcccc          >  1:4003465/1‑101 (MQ=255)                                                                                       cGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGc        <  1:3371885/101‑1 (MQ=255)                                                                                        gAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGcc       <  1:1508141/101‑1 (MQ=255)                                                                                        gAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGcc       >  2:2221/1‑101 (MQ=255)                                                                                        gAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGcc       >  2:4174270/1‑101 (MQ=255)                                                                                        gAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGcc       >  2:1309732/1‑101 (MQ=255)                                                                                         aaaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCa      <  1:3257795/101‑1 (MQ=255)                                                                                         aaaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCa      >  2:2997386/1‑101 (MQ=255)                                                                                         aaaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCa      >  1:175800/1‑101 (MQ=255)                                                                                          aaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAg     >  2:3209898/1‑101 (MQ=255)                                                                                           atcccccgGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgc    <  2:3146932/98‑1 (MQ=255)                                                                                           aTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgc    <  1:5131841/101‑1 (MQ=255)                                                                                            tACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgcg   >  1:3036782/1‑101 (MQ=255)                                                                                            tACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgcg   >  2:2374532/1‑101 (MQ=255)                                                                                             aCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgcgc  >  1:3997683/1‑101 (MQ=255)                                                                                                  |                                                                                                CGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.GGGGT..GTGCG.GAAGCCCCCGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCCGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGC  >  NC_014034/974268‑974456 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG