Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,733,287 2 bp→GC 100% coding (309‑310/1128 nt) tgt → queuine tRNA‑ribosyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,733,2870C→G100.0% 837.5 227S103S (TCC→TCGtgtqueuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (119/107):  ref base (0/0):  total (120/107)
*NC_0140341,733,2880G→C100.0% 843.0 227M104M (GTG→CTG) tgtqueuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (120/107):  ref base (0/0):  total (120/107)

Only 100 of 257 total aligned reads displayed.

AGCGCATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCCGTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGAA  >  NC_014034/1733190‑1733382
                                                                                                 ||                                                                                              
     aTCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGTGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCaa                                                                                         <  2:3315762/101‑1 (MQ=255)
      tCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAg                                                                                        <  1:3397649/101‑1 (MQ=255)
      tCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAg                                                                                        <  1:1046223/101‑1 (MQ=255)
        gcgcgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCt                                                                                      <  1:5292484/101‑1 (MQ=255)
        gcgcgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCt                                                                                      >  1:1594658/1‑101 (MQ=255)
        gcgcgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCt                                                                                      <  2:1484439/101‑1 (MQ=255)
          gcgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGa                                                                                    <  1:649687/101‑1 (MQ=255)
           cgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTgcc                                                                                   >  2:5438043/1‑99 (MQ=255)
           cgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGAc                                                                                   <  1:1112830/101‑1 (MQ=255)
            ggCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGAcc                                                                                  <  1:2252863/101‑1 (MQ=255)
            ggCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGAcc                                                                                  >  2:4493349/1‑101 (MQ=255)
            ggCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGAcc                                                                                  >  1:4145505/1‑101 (MQ=255)
              cTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCga                                                                                <  2:3047214/101‑1 (MQ=255)
              cTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCga                                                                                <  1:1066352/101‑1 (MQ=255)
               tGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgag                                                                               <  2:4169038/101‑1 (MQ=255)
                gggCGTGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGTTCAGCCTTGCTTCTCTGCGCAAGCTGACCgctg                                                                              >  1:1901169/1‑98 (MQ=255)
                 ggCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgagga                                                                             <  1:2378147/101‑1 (MQ=255)
                 ggCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgagga                                                                             >  1:2615082/1‑101 (MQ=255)
                  gCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgaggag                                                                            <  2:2759536/101‑1 (MQ=255)
                  gCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgaggag                                                                            >  2:1363085/1‑101 (MQ=255)
                  gCGGGTTGCACAAGGTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGCTTCAGGTGATGAGACTTGCGTCGCTGCGCAACCTGACCgaggag                                                                            >  2:5655524/1‑101 (MQ=255)
                   cGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGGGCCTTGCTTCGCTGCGCAGGCTGACCGaggagg                                                                           >  1:4991533/1‑101 (MQ=255)
                   cGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGaggagg                                                                           >  1:3275172/1‑101 (MQ=255)
                   cGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGaggagg                                                                           >  1:2972307/1‑101 (MQ=255)
                   cGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGaggagg                                                                           <  1:4601955/101‑1 (MQ=255)
                   cGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGaggagg                                                                           >  1:4423791/1‑101 (MQ=255)
                    gggTTGCACAAGTTCATGAACTGGGGGCGCCCGATCCTGCCCGACTCGGGCGGGATTCAGGCGATGAGCCTTGCTTCGCTGCGCAAGCTGAACTAGGAtgg                                                                          >  2:4257235/1‑101 (MQ=255)
                    gggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAggg                                                                          >  2:3928496/1‑101 (MQ=255)
                     ggTTGCACAAGTTCATGAGCTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGc                                                                         <  2:2087455/101‑1 (MQ=255)
                     ggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGc                                                                         >  2:4624206/1‑101 (MQ=255)
                        tGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTg                                                                      <  1:5663514/101‑1 (MQ=255)
                        tGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTg                                                                      <  1:4873288/101‑1 (MQ=255)
                         gCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGa                                                                     <  2:1720530/101‑1 (MQ=255)
                         gCAAAAGTTCATGAACTGGGAACGCCCGGCGCGGACCGACTGGGGCCGGTTTGAGGTGGTGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGa                                                                     <  2:3563309/101‑1 (MQ=255)
                          cacaAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGAc                                                                    >  2:5674493/1‑101 (MQ=255)
                           acaAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGAcc                                                                   >  2:3141425/1‑101 (MQ=255)
                            caAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCt                                                                  <  1:395844/101‑1 (MQ=255)
                            caAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCt                                                                  <  1:597491/101‑1 (MQ=255)
                             aaGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCtt                                                                 >  2:5499736/1‑101 (MQ=255)
                             aaGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCtt                                                                 <  2:1741643/101‑1 (MQ=255)
                              aGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCttt                                                                <  1:2338994/101‑1 (MQ=255)
                              aGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCttt                                                                <  1:1484986/101‑1 (MQ=255)
                               gTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCtttt                                                               >  1:5414592/1‑101 (MQ=255)
                                  cATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCa                                                            >  2:632333/1‑101 (MQ=255)
                                   aTGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCac                                                           >  2:784639/1‑100 (MQ=255)
                                      aaCTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCa                                                        <  2:2736095/101‑1 (MQ=255)
                                       aCTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCat                                                       >  2:3833487/1‑101 (MQ=255)
                                        cTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCata                                                      <  1:3195263/101‑1 (MQ=255)
                                        cTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCata                                                      <  2:1585329/101‑1 (MQ=255)
                                          gggAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATc                                                    <  2:4656417/101‑1 (MQ=255)
                                           ggAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCg                                                   <  2:403367/101‑1 (MQ=255)
                                            gAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGa                                                  >  1:247655/1‑101 (MQ=255)
                                               cgcCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACgg                                               >  2:3120386/1‑101 (MQ=255)
                                                  ccGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTc                                            >  2:332843/1‑101 (MQ=255)
                                                    gATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTGGa                                          >  2:4879611/1‑101 (MQ=255)
                                                     aTCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGaa                                         >  2:4396169/1‑101 (MQ=255)
                                                     aTCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGaa                                         >  2:4086391/1‑101 (MQ=255)
                                                     aTCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGaa                                         >  2:186743/1‑101 (MQ=255)
                                                     aTCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGGGACCTTTTCCAGCCATATCGACGGCTCGaa                                         >  1:362101/1‑101 (MQ=255)
                                                       ccTGGCCGCCTCGGGCGGGTTTCGGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGGCCGGGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAac                                       <  1:3004770/101‑1 (MQ=255)
                                                        cTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAaca                                      <  2:3688797/101‑1 (MQ=255)
                                                         tggccgtcgcgggcggtTTTCAGGTGATGAGCCTTGCTTCGCTGCGAAAGCTGACCGAGGAGGGCGTGCCCTTTTCCAGCCATATCGACGGCTCGAAacac                                     <  2:2447822/92‑1 (MQ=255)
                                                            ccgccccgggcgggTTTCAGGTGATGAGCCTTGCTTCGCTCCGCAAGCCGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCAt                                  <  1:4257495/95‑1 (MQ=255)
                                                            ccGACTCGGGGGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCAt                                  <  1:3130289/101‑1 (MQ=255)
                                                            ccGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCAt                                  <  1:1831376/101‑1 (MQ=255)
                                                               aCTCGGGCGGGTTTCAGGTGATGAGCCTGGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTg                               <  2:2888257/101‑1 (MQ=255)
                                                                     gcgggTTTCGGGTGATAAGCTTTGCTTCGCTGCGAAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGccc                         <  1:4049379/101‑1 (MQ=255)
                                                                     gcgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGCCCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGccc                         <  1:1696423/101‑1 (MQ=255)
                                                                     gcgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGccc                         <  2:339312/101‑1 (MQ=255)
                                                                     gcgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGccc                         >  2:5541459/1‑101 (MQ=255)
                                                                      cgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCg                        <  2:400112/101‑1 (MQ=255)
                                                                      cgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCg                        <  1:5646615/101‑1 (MQ=255)
                                                                      cgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTCCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCg                        <  2:2975985/101‑1 (MQ=255)
                                                                        ggattCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGaa                      >  1:4274469/4‑101 (MQ=255)
                                                                        ggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGaa                      >  1:5133915/1‑101 (MQ=255)
                                                                           ttCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGc                   >  1:548806/1‑101 (MQ=255)
                                                                            tCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCt                  >  2:3448181/1‑101 (MQ=255)
                                                                            tCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCt                  >  2:1786080/1‑101 (MQ=255)
                                                                            tCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCt                  >  1:4310168/1‑101 (MQ=255)
                                                                            tCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCt                  >  1:5611648/1‑101 (MQ=255)
                                                                             cAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTc                 >  1:3666724/1‑101 (MQ=255)
                                                                             cAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTc                 >  2:3736700/1‑101 (MQ=255)
                                                                             cAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTc                 <  1:2718540/101‑1 (MQ=255)
                                                                              aGGTGATGAGCCTTGCTTCGCTGCGCAGGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCg                <  1:3168798/101‑1 (MQ=255)
                                                                               ggTGATGGGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGa               <  2:5122706/101‑1 (MQ=255)
                                                                               ggTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGa               >  1:5312973/1‑101 (MQ=255)
                                                                                 tgatgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATg             >  1:1168844/1‑101 (MQ=255)
                                                                                   atgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCTATGga           >  1:996776/1‑101 (MQ=255)
                                                                                   atgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGga           >  2:5239562/1‑101 (MQ=255)
                                                                                   atgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTCCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGga           <  2:5288528/101‑1 (MQ=255)
                                                                                     gaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTcagatcgga         >  2:1091757/1‑93 (MQ=255)
                                                                                     gaGCCTTCCTTCGCTGCGCAAGCGGACCGAGGAGGGCGTGCCCTTTTCCAGCCGTATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGgaga         <  2:611506/101‑1 (MQ=255)
                                                                                      acccTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGAt        <  2:897480/99‑1 (MQ=255)
                                                                                      aGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGAt        >  2:504398/1‑101 (MQ=255)
                                                                                      aGCCGTGCTTCGCGGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGAt        <  2:74886/101‑1 (MQ=255)
                                                                                         cTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCa     <  1:4630596/101‑1 (MQ=255)
                                                                                         cTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCa     <  2:2628234/101‑1 (MQ=255)
                                                                                           tGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGa   >  1:3118274/1‑101 (MQ=255)
                                                                                           tGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGa   >  1:3356869/1‑101 (MQ=255)
                                                                                            gCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGGCGCCCGAACGCTCGATGGAGATCCAGaa  >  1:2510594/1‑101 (MQ=255)
                                                                                                 ||                                                                                              
AGCGCATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCCGTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGAA  >  NC_014034/1733190‑1733382

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG