Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,802,277 2 bp→GC 100% coding (660‑661/2124 nt) ligA → DNA ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,802,2770C→G100.0% 738.3 199S220S (TCC→TCGligADNA ligase
Reads supporting (aligned to +/- strand):  new base (76/123):  ref base (0/0):  total (76/123)
*NC_0140341,802,2780G→C100.0% 740.6 199M221M (GTG→CTG) ligADNA ligase
Reads supporting (aligned to +/- strand):  new base (76/123):  ref base (0/0):  total (76/123)

Only 100 of 215 total aligned reads displayed.

TACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACGCA  >  NC_014034/1802182‑1802372
                                                                                               ||                                                                                              
tACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTgcg                                                                                            <  1:2868493/101‑1 (MQ=255)
   aTGAGCGATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCa                                                                                         <  2:2653435/101‑1 (MQ=255)
   aTGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCa                                                                                         <  2:454178/101‑1 (MQ=255)
   aTGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCa                                                                                         <  1:794270/101‑1 (MQ=255)
   aTGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCa                                                                                         <  1:1763327/101‑1 (MQ=255)
      aGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCt                                                                                      <  2:1291653/101‑1 (MQ=255)
      aGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCGTCGCCAATCCGCCCAATGCCGCCCCGGGCTCGCTGCGCCAGCt                                                                                      >  1:386747/1‑101 (MQ=255)
       gCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTc                                                                                     <  2:5745063/101‑1 (MQ=255)
          aTGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGAc                                                                                  >  1:2088731/1‑101 (MQ=255)
          aTGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGAc                                                                                  >  1:2823994/1‑101 (MQ=255)
            gCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTTGGCCAGCTCGCCGc                                                                                >  2:2291940/1‑101 (MQ=255)
            gCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGc                                                                                >  1:3385930/1‑101 (MQ=255)
            gCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGc                                                                                >  1:1117183/1‑101 (MQ=255)
            gCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGc                                                                                <  2:2807451/101‑1 (MQ=255)
               gATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGGGACAAGAGCTTCGCCAATGCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGATGACCg                                                                             >  2:3033643/1‑101 (MQ=255)
                aTTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCgg                                                                            >  1:905001/1‑101 (MQ=255)
                 ttttGAGGCGCTGAATGCGCGGCAGGCAGGGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGGAGCCCGGa                                                                           >  1:2246941/1‑101 (MQ=255)
                   ttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATc                                                                         <  1:2138715/101‑1 (MQ=255)
                    tGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCa                                                                        <  2:283438/101‑1 (MQ=255)
                    tGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCa                                                                        <  1:486963/101‑1 (MQ=255)
                     gcggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAc                                                                       <  1:3979378/101‑1 (MQ=255)
                       ggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCg                                                                     <  2:1909566/101‑1 (MQ=255)
                       ggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCg                                                                     >  2:2123421/1‑101 (MQ=255)
                           cTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTc                                                                 >  2:841965/1‑101 (MQ=255)
                            tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                                                                <  2:2470893/101‑1 (MQ=255)
                             gAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCa                                                               >  2:4629478/1‑101 (MQ=255)
                              aaTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAg                                                              <  1:1071037/101‑1 (MQ=255)
                               aTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGa                                                             <  2:1023757/101‑1 (MQ=255)
                               aTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGa                                                             <  2:2370438/101‑1 (MQ=255)
                                tGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAc                                                            <  1:4019342/101‑1 (MQ=255)
                                tGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAc                                                            >  1:4705696/1‑101 (MQ=255)
                                 gcgcGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAcc                                                           <  2:5217212/101‑1 (MQ=255)
                                   gcgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCCGACCCt                                                         >  1:5749468/1‑101 (MQ=255)
                                   gcgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCt                                                         >  1:3289797/1‑101 (MQ=255)
                                     ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                                                       >  2:1443447/1‑101 (MQ=255)
                                     ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                                                       <  2:3342914/101‑1 (MQ=255)
                                      gCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGa                                                      <  1:2475549/101‑1 (MQ=255)
                                      gCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGa                                                      <  2:4493996/101‑1 (MQ=255)
                                       cAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaa                                                     <  1:831696/101‑1 (MQ=255)
                                        aGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaaa                                                    <  1:2991244/101‑1 (MQ=255)
                                        aGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaaa                                                    >  2:4183678/1‑101 (MQ=255)
                                           gggAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTc                                                 <  1:1451047/100‑1 (MQ=255)
                                           cGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATAATCGTCTCAAGACCCTTGAAATTc                                                 >  2:928040/1‑101 (MQ=255)
                                            ggAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCt                                                <  2:1772747/101‑1 (MQ=255)
                                            ggAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCt                                                >  1:2197640/1‑101 (MQ=255)
                                             gAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCtt                                               >  1:5656625/1‑101 (MQ=255)
                                             gAGCGGGGCGACAAGAGCCTCGCCAATCCGCGCAACGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCtt                                               <  2:5788902/101‑1 (MQ=255)
                                              aGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCttt                                              >  1:5769502/1‑101 (MQ=255)
                                              aGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCttt                                              >  2:1233039/1‑101 (MQ=255)
                                               gCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTg                                             <  2:1218670/101‑1 (MQ=255)
                                                cGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGc                                            >  1:5464978/1‑101 (MQ=255)
                                                  gggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCt                                          <  1:2793014/101‑1 (MQ=255)
                                                   ggCGACAAGAGCTTCGCCACTCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTa                                         <  1:536825/101‑1 (MQ=255)
                                                   ggCGACAAGAGCCACGACAATACGCGCAGTGCCGCCGCGGGCTCGCTACGCCATCGCGACGCCCGGATCACCGCCGCCAGACCCTTGAAAGTCTTTGCCTa                                         <  2:2246815/101‑1 (MQ=255)
                                                     ccaCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt                                       <  1:1345403/99‑1 (MQ=255)
                                                     cGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt                                       <  2:5185229/101‑1 (MQ=255)
                                                     cGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt                                       <  2:5331303/101‑1 (MQ=255)
                                                     cGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt                                       <  2:4325960/101‑1 (MQ=255)
                                                      gACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTc                                      <  1:992691/101‑1 (MQ=255)
                                                       aCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTcc                                     >  1:1571499/1‑101 (MQ=255)
                                                       aCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTcc                                     >  1:339600/1‑101 (MQ=255)
                                                        cAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCt                                    >  2:2082422/1‑101 (MQ=255)
                                                        cAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCt                                    <  1:1293819/101‑1 (MQ=255)
                                                        cAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCt                                    >  2:4163686/1‑101 (MQ=255)
                                                         aagagcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTg                                   >  2:3110220/7‑101 (MQ=255)
                                                          agagcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTgg                                  <  2:2719542/96‑1 (MQ=255)
                                                           gagcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTggg                                 >  2:4855248/5‑101 (MQ=255)
                                                           gagcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTggg                                 <  1:5069920/97‑1 (MQ=255)
                                                            ggcttCGCCAATCCGCGCAATCCCGCCGCGGGCTCGCTGCACCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTgggg                                <  2:1871891/98‑1 (MQ=255)
                                                            agcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTgggg                                <  2:741527/98‑1 (MQ=255)
                                                                tCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGtt                            <  1:2533009/101‑1 (MQ=255)
                                                                 cGCCAATCCGCGCAATGCCGCAGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTc                           <  1:667774/101‑1 (MQ=255)
                                                                  gCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCt                          >  1:2683123/1‑101 (MQ=255)
                                                                  gCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGAGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCt                          <  1:5018945/101‑1 (MQ=255)
                                                                   ccAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTg                         <  2:401001/101‑1 (MQ=255)
                                                                   ccAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTGCTg                         >  2:3865333/1‑101 (MQ=255)
                                                                   ccAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGCTCTg                         <  2:4898364/101‑1 (MQ=255)
                                                                     aaTCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTc                       >  1:3668487/1‑101 (MQ=255)
                                                                       tcggcgcAACGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCg                     <  2:5571712/98‑1 (MQ=255)
                                                                       tCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCg                     <  1:3194332/101‑1 (MQ=255)
                                                                        ccGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGa                    <  1:5161047/101‑1 (MQ=255)
                                                                          gcgcAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAAc                  <  2:3614689/101‑1 (MQ=255)
                                                                          gcgcAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAAc                  <  1:1819081/101‑1 (MQ=255)
                                                                             cAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGc               <  1:1868229/101‑1 (MQ=255)
                                                                             cAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGc               >  1:3208026/1‑101 (MQ=255)
                                                                             cAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGc               <  1:4162629/101‑1 (MQ=255)
                                                                              aaTGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGct              >  1:4130910/1‑101 (MQ=255)
                                                                              aaTGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCT.GGGCGTTCTGTCCGAACCGctc             >  2:1395583/1‑101 (MQ=255)
                                                                                tctcgccgcGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGctct            <  1:4580331/98‑1 (MQ=255)
                                                                                tGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGctct            <  1:5321567/101‑1 (MQ=255)
                                                                                 gccgccGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCtctc           >  1:937874/1‑101 (MQ=255)
                                                                                 gccgccGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCtctc           <  1:3161826/101‑1 (MQ=255)
                                                                                 gccgccGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCtctc           <  2:2787796/101‑1 (MQ=255)
                                                                                  ccgccgCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCg          <  2:5769207/101‑1 (MQ=255)
                                                                                   cgccgcGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCgg         >  1:1278542/1‑101 (MQ=255)
                                                                                      cgcgGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGaca      >  2:1349662/1‑101 (MQ=255)
                                                                                       gcgGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGacac     <  2:3668259/101‑1 (MQ=255)
                                                                                        cgGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACg    >  2:3234491/1‑101 (MQ=255)
                                                                                         gggCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACGc   >  1:77534/1‑101 (MQ=255)
                                                                                          ggCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACGCa  >  1:3521291/1‑101 (MQ=255)
                                                                                               ||                                                                                              
TACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACGCA  >  NC_014034/1802182‑1802372

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG