Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,959,054 2 bp→GC 100% coding (474‑475/1218 nt) RCAP_rcc01811 ← major facilitator superfamily protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,959,0540C→G100.0% 953.8 250A159P (GCT→CCT) RCAP_rcc01811major facilitator superfamily protein
Reads supporting (aligned to +/- strand):  new base (131/119):  ref base (0/0):  total (131/119)
*NC_0140341,959,0550G→C100.0% 937.5 250A158A (GCC→GCG) RCAP_rcc01811major facilitator superfamily protein
Reads supporting (aligned to +/- strand):  new base (131/119):  ref base (0/0):  total (131/119)

Only 100 of 255 total aligned reads displayed.

GCTCAGCCAGGCCAATCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGCGGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTCGCGCACCAGCGCCATCG  >  NC_014034/1958959‑1959149
                                                                                               ||                                                                                              
gCTCAGCCAGGCCAATCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCaa                                                                                            >  2:2713098/1‑101 (MQ=255)
 cTCAGCCAGGCCAATCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAg                                                                                           <  2:2618802/101‑1 (MQ=255)
   cAGCCAGGCCAATCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAgcg                                                                                         <  1:5566617/101‑1 (MQ=255)
   cAGCCAGGCCAATCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAgcg                                                                                         <  2:66544/101‑1 (MQ=255)
    aGCCAGGCCAATCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAgcgc                                                                                        >  1:2812326/1‑101 (MQ=255)
      ccAGGCCAATCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCg                                                                                      >  1:1213986/1‑101 (MQ=255)
      ccAGGCCAATCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCg                                                                                      >  2:2105760/1‑101 (MQ=255)
      ccAGGCCAATCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCg                                                                                      >  1:2341160/1‑101 (MQ=255)
       cAGGCCAATCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCgg                                                                                     >  1:2751718/1‑101 (MQ=255)
        aGGCCAATCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGc                                                                                    >  1:1483991/1‑101 (MQ=255)
        aGGCCAATCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGc                                                                                    >  2:5023688/1‑101 (MQ=255)
          gCCAATCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCAc                                                                                  <  1:1956205/101‑1 (MQ=255)
            cAATCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCa                                                                                >  2:5440799/1‑101 (MQ=255)
             aaTCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAg                                                                               >  2:2998006/1‑101 (MQ=255)
                ccGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGAt                                                                            >  2:3834364/1‑101 (MQ=255)
                 cGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATg                                                                           >  2:1579144/1‑101 (MQ=255)
                 cGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATg                                                                           >  1:5674076/1‑101 (MQ=255)
                  gATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGa                                                                          <  1:4372297/101‑1 (MQ=255)
                      aGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAAc                                                                      <  2:3453844/101‑1 (MQ=255)
                      aGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAAc                                                                      >  1:3002628/1‑101 (MQ=255)
                       gcgcGAAGAGGATGAAGGCATAGCACACGCCGCGCCATCCCGCGCAACCGATAATCAGCTGTCCGGCCGACGGCGCAAGCGCCGGCACCAGGATGCAAAcc                                                                     >  2:1859886/1‑101 (MQ=255)
                        cgcgAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACca                                                                    <  1:5469313/101‑1 (MQ=255)
                        cgcgAAGAGGATGAAGGCATAGAACACACCGCGCCATCCCGCGAAACCGATAATCAGCTGTCTGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACca                                                                    >  1:2879843/1‑101 (MQ=255)
                         gcgAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACcat                                                                   <  2:21255/101‑1 (MQ=255)
                         gcgAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACcat                                                                   <  1:5193116/101‑1 (MQ=255)
                          cgAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACcatc                                                                  >  1:5382629/1‑101 (MQ=255)
                          cgAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACcatc                                                                  >  2:2510413/1‑101 (MQ=255)
                           gAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACcatca                                                                 <  2:4546276/101‑1 (MQ=255)
                           gAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACcatca                                                                 >  2:3096036/1‑101 (MQ=255)
                           gAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACcatca                                                                 >  2:2481264/1‑101 (MQ=255)
                           gAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGACCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACcatca                                                                 >  2:2829929/1‑101 (MQ=255)
                            aaGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACcatcat                                                                <  2:5580717/101‑1 (MQ=255)
                            aaGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACcatcat                                                                <  1:1737198/101‑1 (MQ=255)
                            aaGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACcatcat                                                                <  1:5131314/101‑1 (MQ=255)
                             agagGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCatg                                                               >  2:3952275/1‑101 (MQ=255)
                             agagGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCatg                                                               >  2:4110861/1‑101 (MQ=255)
                              gagGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCatga                                                              <  1:4028212/101‑1 (MQ=255)
                              gagGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCatga                                                              >  1:3638366/1‑101 (MQ=255)
                              gagGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCatga                                                              <  2:4385958/101‑1 (MQ=255)
                                ggATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCatgatg                                                            <  1:4248702/101‑1 (MQ=255)
                                   tGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAg                                                         <  1:2178635/101‑1 (MQ=255)
                                    gAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAgg                                                        >  1:3754604/1‑101 (MQ=255)
                                    gAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAgg                                                        >  1:2020794/1‑101 (MQ=255)
                                    gAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAgg                                                        >  1:1006200/1‑101 (MQ=255)
                                     aaGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGc                                                       <  2:3345811/101‑1 (MQ=255)
                                       ggCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCgg                                                     >  2:5240172/1‑101 (MQ=255)
                                       ggCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCgg                                                     <  1:5609653/101‑1 (MQ=255)
                                        gCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCggc                                                    >  2:1781908/1‑100 (MQ=255)
                                         cATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTc                                                   <  2:848497/101‑1 (MQ=255)
                                         cATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTc                                                   <  1:3224052/101‑1 (MQ=255)
                                           tAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCAc                                                 >  2:1074166/1‑101 (MQ=255)
                                           tAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCAc                                                 <  2:3565585/101‑1 (MQ=255)
                                             gAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCGGGATGAAAACCATCATGATGAAGGCGGTCACCt                                               >  1:4984453/1‑101 (MQ=255)
                                              aaCACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCTGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCtt                                              >  1:1794796/1‑101 (MQ=255)
                                               acacGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTg                                             >  1:3798451/1‑101 (MQ=255)
                                                 acGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGc                                           >  1:1386902/1‑101 (MQ=255)
                                                 acGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGc                                           >  1:4349048/1‑101 (MQ=255)
                                                  cgccgcGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGcc                                          <  1:2480145/101‑1 (MQ=255)
                                                   gccgcGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCa                                         <  2:4557151/101‑1 (MQ=255)
                                                   gccgcGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCa                                         <  2:2931747/101‑1 (MQ=255)
                                                    ccgcGCCATCCCGCCAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCAt                                        <  2:3806068/101‑1 (MQ=255)
                                                        gcCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCa                                    >  2:4247213/1‑101 (MQ=255)
                                                         ccATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCAc                                   >  1:2093412/1‑101 (MQ=255)
                                                         ccATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCAc                                   >  1:2473935/1‑101 (MQ=255)
                                                         ccATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCAc                                   <  2:4348782/101‑1 (MQ=255)
                                                         ccATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCAc                                   <  1:607962/101‑1 (MQ=255)
                                                           aTCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGc                                 <  2:5701920/101‑1 (MQ=255)
                                                           aTCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGc                                 >  2:1362570/1‑101 (MQ=255)
                                                           aTCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGc                                 >  1:4229979/1‑101 (MQ=255)
                                                            tCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGcc                                >  2:5345512/1‑101 (MQ=255)
                                                             cccGCGAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGccc                               <  1:4544723/101‑1 (MQ=255)
                                                               cgcgAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCgg                             <  1:2970633/101‑1 (MQ=255)
                                                                gcgAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGa                            >  1:3590415/1‑101 (MQ=255)
                                                                  gAAACCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAAt                          <  1:4800935/101‑1 (MQ=255)
                                                                   aacacGATAAACAGCTGTCCGGCTGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATa                         <  2:3754376/97‑1 (MQ=255)
                                                                   aaaCCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATa                         >  2:596215/1‑101 (MQ=255)
                                                                   aaaCCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATa                         <  1:4144368/101‑1 (MQ=255)
                                                                    aaCCGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATAc                        <  2:1213903/101‑1 (MQ=255)
                                                                       cGATAATCAGCTTTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATg                     <  2:1970252/101‑1 (MQ=255)
                                                                       cGATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATg                     >  1:787327/1‑101 (MQ=255)
                                                                        gATAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGt                    >  1:1652754/1‑101 (MQ=255)
                                                                         aTAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTc                   <  2:4928886/101‑1 (MQ=255)
                                                                          tAATCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTcg                  >  1:2616010/1‑101 (MQ=255)
                                                                           aaTCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACagatcgg                 >  2:5645707/1‑95 (MQ=255)
                                                                           aaTCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTcgc                 <  1:1615242/101‑1 (MQ=255)
                                                                            aTCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGTCGGTCACCTTGGCCATTTCACGCCCGGATTACANGNngng                >  1:999204/1‑101 (MQ=255)
                                                                            aTCAGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCGCCTTGGCCATTTCACGCCCGGAATACATGTcgcg                <  1:4659717/101‑1 (MQ=255)
                                                                              cAGATGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTCGCGCa              >  2:5143836/1‑101 (MQ=255)
                                                                               aGCTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTCGCGCAc             <  1:2211519/101‑1 (MQ=255)
                                                                                 cTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTCGCGCACCa           >  2:117037/1‑101 (MQ=255)
                                                                                 cTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTCGCGCACCa           <  1:2415880/101‑1 (MQ=255)
                                                                                 cTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTCGCGCACCa           <  2:2341034/101‑1 (MQ=255)
                                                                                 cTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTCGCGCACCa           >  2:2440278/1‑101 (MQ=255)
                                                                                 cTGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCACCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTCGCGCACCa           >  2:4891827/1‑101 (MQ=255)
                                                                                  tGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTCGCGCACCAg          <  2:3188650/101‑1 (MQ=255)
                                                                                  tGTCCGGCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTCGCGCACCAg          >  1:3379065/1‑101 (MQ=255)
                                                                                        gCCGAAGGCGCAAGCGCCGGCACCAGGATGAAATCCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTCGCGCACCAGCGCCAt    <  1:4970573/101‑1 (MQ=255)
                                                                                        gCCGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTCGCGCACCAGCGCCAt    <  1:4065378/101‑1 (MQ=255)
                                                                                         ccGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTCGCGCACCAGCGCCATc   >  1:2685372/1‑101 (MQ=255)
                                                                                          cGAAGGCGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTCGCGCACCAGCGCCATCg  >  2:4660690/1‑101 (MQ=255)
                                                                                               ||                                                                                              
GCTCAGCCAGGCCAATCCGATCAGCGCGAAGAGGATGAAGGCATAGAACACGCCGCGCCATCCCGCGAAACCGATAATCAGCTGTCCGGCCGAAGCGGCAAGCGCCGGCACCAGGATGAAAACCATCATGATGAAGGCGGTCACCTTGGCCATTTCACGCCCGGAATACATGTCGCGCACCAGCGCCATCG  >  NC_014034/1958959‑1959149

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG