Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,900 2 bp→CA 100% coding (2674‑2675/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,9000A→C100.0% 771.2 221V892G (GTA→GGA) RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (96/124):  ref base (1/0):  total (97/124)
*NC_0140342,304,9010C→A100.0% 760.6 221V892L (GTA→TTA) RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (96/123):  ref base (1/0):  total (97/124)

Only 100 of 230 total aligned reads displayed.

ATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCAT.GC.G.AAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTG  >  NC_014034/2304809‑2304988
                                                                                               ||                                                                                        
aTAATCTTGCGAAAAACTCGGCCGGATCCGGCCTGTCACCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCg                                                                                   <  1:633876/101‑1 (MQ=255)
 tAATCTTGCGCAAACCCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGc                                                                                  <  1:5607326/101‑1 (MQ=255)
 tAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGc                                                                                  >  1:871051/1‑101 (MQ=255)
 aaaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGc                                                                                  <  2:1687221/100‑1 (MQ=255)
  aaTCTTGCGCAAACCTCGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCa                                                                                 <  1:400176/101‑1 (MQ=255)
  aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGcc                                                                                 >  1:2322018/1‑100 (MQ=255)
    tCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGGG.G.AAGACCGCCGTCAGCCCCCGCA.gg                                                                              >  2:4624213/1‑101 (MQ=255)
      ttGCGCAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCA.gggt                                                                            <  2:1656211/101‑3 (MQ=255)
      ttGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCA.gggt                                                                            <  1:3798024/101‑3 (MQ=255)
      ttGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCA.gggt                                                                            >  2:2134981/1‑99 (MQ=255)
       tGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCA.gggtc                                                                           >  1:3016663/1‑98 (MQ=255)
       tGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCA.gggtc                                                                           >  2:4310340/1‑98 (MQ=255)
       tGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCA.gggtc                                                                           >  2:3493391/1‑98 (MQ=255)
         cgcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGGCAGCTCCCGCA.gggcctg                                                                         >  1:3325603/1‑96 (MQ=255)
           cAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTgcg                                                                        >  2:3053588/1‑101 (MQ=255)
           aaaaccccgcccGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTgcg                                                                        <  2:1977312/91‑1 (MQ=255)
            aaaaCTCGGCCGGATCCGGCCTGTCATCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCC.GCAT.GCGG.AAGACCGCCGTCAGCTCCCGCA.ggggctgcgc                                                                      >  2:5517476/1‑93 (MQ=255)
            aaaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTgcgc                                                                       >  2:5702246/1‑101 (MQ=255)
             aaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGAGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGGGcc                                                                      >  2:1932460/1‑101 (MQ=255)
               aCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGGGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTc                                                                    >  2:5229265/1‑101 (MQ=255)
                cTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGGCTGCGCCTCg                                                                   >  2:1302870/1‑101 (MQ=255)
                cTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGCCAGCTCCCGCAGGGTCTGCGCCTCg                                                                   >  1:1382428/1‑101 (MQ=255)
                 tCGGCGGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGc                                                                  <  1:1571110/101‑1 (MQ=255)
                  cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGcc                                                                 >  1:1706085/1‑101 (MQ=255)
                   ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                                >  2:92961/1‑101 (MQ=255)
                   ggCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                                <  1:5702541/101‑1 (MQ=255)
                   ggCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                                <  1:4287956/101‑1 (MQ=255)
                    gCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCca                                                               <  2:1565542/101‑1 (MQ=255)
                       tgccccGGCCTGTAAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagca                                                            <  1:2525911/97‑1 (MQ=255)
                       ggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagca                                                            >  1:4915432/1‑101 (MQ=255)
                        gacccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagcag                                                           <  1:5426120/98‑1 (MQ=255)
                        gATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagcag                                                           >  1:2773021/1‑101 (MQ=255)
                        gATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagcag                                                           >  1:4453286/1‑101 (MQ=255)
                          tCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAgcg                                                         >  2:3131454/1‑101 (MQ=255)
                           ccGGCCTGGCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAgcgc                                                        <  1:5037421/101‑1 (MQ=255)
                            cGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGcc                                                       >  1:1928471/1‑101 (MQ=255)
                             ggCCTGACCGCCGGCCTGGCGCCACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGCCCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                      <  1:4603959/101‑1 (MQ=255)
                              gCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                     >  1:3376419/1‑101 (MQ=255)
                              gCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                     >  1:1597296/1‑101 (MQ=255)
                              gCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                     <  1:2141017/101‑1 (MQ=255)
                              gCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                     <  2:762877/101‑1 (MQ=255)
                               ccTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGc                                                    >  1:5488779/1‑101 (MQ=255)
                                 tGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGt                                                  >  1:1446985/1‑101 (MQ=255)
                                   tCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcg                                                >  1:973092/1‑101 (MQ=255)
                                   tCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcg                                                <  1:10814/101‑1 (MQ=255)
                                     aGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgcg                                              <  1:4125477/101‑1 (MQ=255)
                                      gCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCgg                                             <  1:2361088/101‑1 (MQ=255)
                                       ccGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGc                                            >  1:5182275/1‑101 (MQ=255)
                                       ccGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGc                                            >  2:3929316/1‑101 (MQ=255)
                                         ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                          <  2:900575/101‑1 (MQ=255)
                                           ccTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcg                                        <  1:3681941/101‑1 (MQ=255)
                                            cTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGCCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcgg                                       >  1:3407257/1‑100 (MQ=255)
                                             tGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtt                                      <  2:4062365/101‑1 (MQ=255)
                                             tGCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtt                                      >  1:1367949/1‑101 (MQ=255)
                                              gCCGCAACGGCGGCTCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                                     <  2:4519998/101‑1 (MQ=255)
                                              gCCGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                                     >  1:1013549/1‑101 (MQ=255)
                                               ccGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtttt                                    <  2:443093/101‑1 (MQ=255)
                                               ccGCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTAAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtttt                                    >  1:4631187/1‑101 (MQ=255)
                                                 gCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTcc                                  <  1:2811308/101‑1 (MQ=255)
                                                 gCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTcc                                  >  1:5277980/1‑101 (MQ=255)
                                                 gCAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTcc                                  <  1:5517768/101‑1 (MQ=255)
                                                  cAACGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                 <  1:1327213/101‑1 (MQ=255)
                                                   aaCGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                <  2:1496069/101‑1 (MQ=255)
                                                   aaCGGCGGCGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                <  1:2740680/101‑1 (MQ=255)
                                                      ggcggcGCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                             >  1:3448750/1‑101 (MQ=255)
                                                       gcggcgCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGacc                            >  2:5424914/1‑99 (MQ=255)
                                                       gcggcgCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                            <  2:1380214/101‑1 (MQ=255)
                                                       gcggcgCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                            <  2:2301115/101‑1 (MQ=255)
                                                       gcggcgCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                            <  1:3173849/101‑1 (MQ=255)
                                                        cggcgCCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACa                           <  1:3856483/101‑1 (MQ=255)
                                                          gcgcCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATc                         <  1:5747159/101‑1 (MQ=25)
                                                          gcgcCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTCTCCTCGAACATc                         <  1:3131643/101‑1 (MQ=255)
                                                           cgcCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                        <  1:4422480/101‑1 (MQ=25)
                                                           cgcCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                        <  2:3278643/101‑1 (MQ=25)
                                                           cgcCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                        <  1:2588933/101‑1 (MQ=25)
                                                            gcCGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCTGCTTCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgc                       <  1:4951710/101‑1 (MQ=25)
                                                              cGGTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                     >  2:1472757/1‑101 (MQ=25)
                                                               ggTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                    <  1:4824066/101‑1 (MQ=25)
                                                               ggTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                    >  2:5117339/1‑101 (MQ=25)
                                                               ggTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                    >  2:1157027/1‑101 (MQ=25)
                                                                gTGGCG.CCTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTNCCTCGAACATCGCCGCa                   >  2:488714/1‑101 (MQ=25)
                                                                  gggcgccTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                  <  1:3929560/96‑1 (MQ=25)
                                                                   ggcgccTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                 <  2:3578520/97‑1 (MQ=25)
                                                                   ggcgccTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                 <  2:3726950/97‑1 (MQ=25)
                                                                    gcgccTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                <  1:4332658/98‑1 (MQ=25)
                                                                    gcgccTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                <  2:5189153/98‑1 (MQ=25)
                                                                     ctccTGCAT.GC.GAAATACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt               <  2:1811488/99‑1 (MQ=21)
                                                                     cgccTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt               <  1:4526326/99‑1 (MQ=25)
                                                                     cgccTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt               <  1:622069/99‑1 (MQ=25)
                                                                     cgccTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt               <  2:1705986/99‑1 (MQ=25)
                                                                      gccTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg              <  2:2203599/100‑1 (MQ=25)
                                                                       ccTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGc             <  1:849758/101‑1 (MQ=25)
                                                                        cTGCAT.GCGG.AAGCCCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt            <  1:1208033/101‑1 (MQ=21)
                                                                        cTGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt            <  2:4187263/101‑1 (MQ=25)
                                                                         tGCAT.GCGG.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg           <  1:291222/101‑1 (MQ=25)
                                                                             atgcgg.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGt        <  2:2749022/96‑1 (MQ=21)
                                                                              tgcgg.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTg       >  2:5759102/5‑101 (MQ=21)
                                                                                cgg.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAc     >  2:2556781/3‑101 (MQ=21)
                                                                                 gg.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGAGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAcc    >  2:4986973/2‑101 (MQ=21)
                                                                                  g.AAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCt   >  2:4227833/1‑101 (MQ=21)
                                                                                               ||                                                                                        
ATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCAT.GC.G.AAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTG  >  NC_014034/2304809‑2304988

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG