Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 531,029 2 bp→GC 100% intergenic (+107/+33) valS → / ← RCAP_rcc00497 valyl‑tRNA synthetase/UbiA prenyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034531,0290C→G100.0% 752.6 215intergenic (+107/+34)valS/RCAP_rcc00497valyl‑tRNA synthetase/UbiA prenyltransferase
Reads supporting (aligned to +/- strand):  new base (130/85):  ref base (0/0):  total (130/85)
*NC_014034531,0300G→C100.0% 751.6 215intergenic (+108/+33)valS/RCAP_rcc00497valyl‑tRNA synthetase/UbiA prenyltransferase
Reads supporting (aligned to +/- strand):  new base (130/83):  ref base (0/2):  total (130/85)

Only 100 of 231 total aligned reads displayed.

TCGGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTCGGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGCTCAGCGTCAT  >  NC_014034/530949‑531128
                                                                                ||                                                                                                     
ttcgCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCcc.gc                                                                                 >  2:1020218/4‑101 (MQ=255)
ttcgCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCcc.gc                                                                                 >  1:5217771/4‑101 (MQ=255)
 tcgCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.GGGGAAA.CCCcc.gcc                                                                                >  1:3782198/3‑101 (MQ=25)
   gCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAAGATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCcc.gccgc                                                                              >  1:4888884/1‑101 (MQ=255)
    cTCCCCCCGGGATATTTTTGGCAAGATAAAGGCCCGGAAGCCCCCCTTTATCTTTCCGGAAATATCCCAAGGGGGTGCGGGGG.GGGGAAA.CCCcc.cccccc                                                                             >  1:1055621/1‑101 (MQ=37)
     tCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCcc.gccgccg                                                                            >  1:2875734/1‑101 (MQ=255)
       cccccGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCcc.gccgccgcc                                                                          >  1:2096175/1‑101 (MQ=255)
         cccGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCcc.gccgccgccgc                                                                        <  2:5770178/101‑1 (MQ=255)
          ccGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCcc.gccgccgccgcc                                                                       <  1:2532321/101‑1 (MQ=255)
            gggATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCgg                                                                     <  2:4608385/101‑1 (MQ=255)
            gggATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCgg                                                                     >  1:682069/1‑101 (MQ=255)
            gggATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCgg                                                                     >  1:3365194/1‑101 (MQ=255)
               atatTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATc                                                                  <  1:370205/101‑1 (MQ=255)
               atatTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATc                                                                  <  1:2377530/101‑1 (MQ=255)
                ttttttCGGCAAAAAAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCa                                                                 <  1:4087291/99‑1 (MQ=25)
                tatTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCa                                                                 >  1:4620374/1‑101 (MQ=255)
                tatTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCa                                                                 <  2:3544709/101‑1 (MQ=255)
                tatTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCa                                                                 >  2:4767343/1‑101 (MQ=255)
                tatTTCCGGAAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAGGGGGGGGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCa                                                                 <  2:3560842/101‑1 (MQ=21)
                 atTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCat                                                                >  1:3382514/1‑100 (MQ=255)
                  ttttCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAgc                                                               >  2:4498841/1‑101 (MQ=255)
                   tttCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAgcc                                                              >  1:3566574/1‑101 (MQ=255)
                   tttCGGCAAGAT.AAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGGGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAaccg                                                             >  2:2324635/1‑101 (MQ=21)
                          aaGATANAGGCCGGGAACCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCAGCCGGATCAGCCGCCCATc                                                       <  1:2300789/101‑1 (MQ=21)
                          aaGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATc                                                       >  2:339592/1‑101 (MQ=255)
                          aaGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATc                                                       >  2:134905/1‑101 (MQ=255)
                          aaGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATc                                                       >  2:1263145/1‑101 (MQ=255)
                          aaGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATc                                                       >  2:1157590/1‑101 (MQ=255)
                           aGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATcc                                                      >  2:1730646/1‑101 (MQ=255)
                             aTACAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.GGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCg                                                    >  2:1523355/1‑101 (MQ=25)
                             aTAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCg                                                    >  1:127260/1‑101 (MQ=255)
                               aaaGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGaa                                                  >  2:3690503/1‑101 (MQ=255)
                               aaaGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGaa                                                  <  1:2022527/101‑1 (MQ=255)
                                 aGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAAcc                                                >  1:4482421/1‑101 (MQ=255)
                                     cgggAACCCTCCCTTTATCTTGCCGGAAATACCCCAGGGGGGGGCGGGGG.TGTGAAC.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTg                                            <  2:3293060/99‑1 (MQ=21)
                                      cGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTgc                                           >  2:391772/1‑101 (MQ=255)
                                       ggAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTgcg                                          >  1:5632788/1‑101 (MQ=255)
                                       ggAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTgcg                                          >  2:5158588/1‑101 (MQ=255)
                                       ggAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTgcg                                          >  1:2285382/1‑101 (MQ=255)
                                        gAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTgcgc                                         <  2:5567750/101‑1 (MQ=255)
                                        gAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTgcgc                                         >  1:5174995/1‑101 (MQ=255)
                                        gAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTgcgc                                         >  1:5085540/1‑101 (MQ=255)
                                          aGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGccc                                       <  2:79186/101‑1 (MQ=255)
                                          aGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGccc                                       >  1:3265884/1‑101 (MQ=255)
                                           gCCTCCCTTTATCTTGCCGGAAATATCCCAGGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCa                                      <  1:388720/101‑1 (MQ=255)
                                           gCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCa                                      >  1:245658/1‑101 (MQ=255)
                                           gCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCa                                      <  1:317956/101‑1 (MQ=255)
                                             cTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGCTCAGCCGCCCATCCCGAACCATTGCGCCCaaa                                    >  1:3547021/1‑101 (MQ=255)
                                             cTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCaaa                                    >  2:5622578/1‑101 (MQ=255)
                                               cccTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.GGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAgg                                  >  1:4737444/1‑101 (MQ=255)
                                                 cTTTATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGcc                                >  2:4476249/1‑101 (MQ=255)
                                                   ttATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGNCCATCCCGAACCATTGCGCCCAAAGGCccg                              >  1:1026833/1‑101 (MQ=255)
                                                   ttATCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCccg                              >  2:2010749/1‑101 (MQ=255)
                                                     aTCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAC.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCccgcc                            <  1:1628810/101‑1 (MQ=255)
                                                     aTCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCccgcc                            >  2:1591471/1‑101 (MQ=255)
                                                     aTCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCccgcc                            >  1:1770682/1‑101 (MQ=255)
                                                      tCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCccgccg                           >  1:1972090/1‑101 (MQ=255)
                                                      tCTTGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCccgccg                           >  2:1877728/1‑101 (MQ=255)
                                                        ttGCCGGAAATATCCCAAGGGGGTGCTGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCgccgcc                         >  1:2579697/1‑101 (MQ=255)
                                                         tGCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGccc                        >  2:3425917/1‑101 (MQ=255)
                                                         tGCCGGAAATACCCCAGGGGGGTGCGGGGG.TTTGAAC.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGccc                        <  2:4888603/101‑1 (MQ=255)
                                                          gcgggAAATGCCCCAAGGGGGTGCGGGGG.TGTGAAC.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCa                       <  2:4198288/98‑1 (MQ=37)
                                                          gcaggAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCAGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCa                       <  2:4819654/98‑1 (MQ=37)
                                                          gCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAC.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCa                       <  1:5700479/101‑1 (MQ=255)
                                                          gCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCa                       <  1:5612689/101‑1 (MQ=255)
                                                          gCCGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCa                       >  2:3187725/1‑101 (MQ=255)
                                                           ccGGAAATATCCCAAGGGGGTGCGGGGG.TGTGACA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAg                      <  1:4980303/101‑1 (MQ=255)
                                                           ccGGAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAg                      <  1:759154/101‑1 (MQ=255)
                                                            cgaaaaTATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGa                     <  2:2487167/98‑1 (MQ=255)
                                                            cagaaaatccccAAGGGGGGTGGGGGG.TTTTAAACCCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAg                      <  1:2990397/92‑1 (MQ=255)
                                                             ggAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGaa                    <  1:340170/101‑1 (MQ=255)
                                                             ggAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGaa                    <  2:3681238/101‑1 (MQ=255)
                                                              gAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAAc                   >  2:615881/1‑101 (MQ=255)
                                                              gAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAAc                   >  1:5112760/1‑101 (MQ=255)
                                                              gAAATATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATAGCGCCCAAAGGCCCGCCGCCCAGAAc                   >  1:3478545/1‑101 (MQ=255)
                                                                aaTATCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACAt                 <  2:1805278/101‑1 (MQ=255)
                                                                aaTACCCCAAGGGGGTGCGGGGG.TGTGAAC.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACAt                 <  2:2806559/101‑1 (MQ=38)
                                                                 atatCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATc                >  1:3587234/1‑101 (MQ=255)
                                                                  tatCCCAGGGGGGTGCGGGGG.TGTGAAC.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCCTCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCa               <  2:2958405/101‑1 (MQ=37)
                                                                  tatCCCAAGGGGGTGCGGGGG.TGTGAAC.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCa               <  2:2685782/101‑1 (MQ=255)
                                                                  tatCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCa               >  2:4027499/1‑101 (MQ=255)
                                                                   atCCCAGGGGGGTGCGGGGG.TGTGAAC.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAg              <  2:2096058/101‑1 (MQ=38)
                                                                   atCCCAAGGGGGTGCGGGGG.TGTGAAC.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAg              <  1:1018808/101‑1 (MQ=255)
                                                                   atCCCAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGGCGCCGCCGAATCAGCCGCCCATCCCTAACGATTGCGCCCCAGGGGCAGCCGCCCAGAACTTCAg              >  2:307604/1‑101 (MQ=21)
                                                                     ccgaggggggTGCGGGGG.TGTG..A.CCCCC..CCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGCTCa         <  2:5414583/96‑1 (MQ=37)
                                                                      ccAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGCt           >  1:1439900/1‑101 (MQ=255)
                                                                      ccAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGCt           >  1:5339784/1‑101 (MQ=255)
                                                                      ccAAGGGGGGCGGGGGGTTGAAAAC.CCCCC.CCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGc            <  2:4975739/101‑1 (MQ=255)
                                                                      ccAAGGGGGGCCGGGGG.TGCGAAC.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGCCCCGCCGCCCAGAACATCAGGCt           <  2:2579555/101‑1 (MQ=255)
                                                                       cAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGCTc          >  1:1720854/1‑101 (MQ=255)
                                                                       cAAGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGCTc          >  1:2780886/1‑101 (MQ=255)
                                                                        aaGGGGGTGCGGGGG.TGTGAAC.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGCTCa         <  2:5217484/101‑1 (MQ=255)
                                                                        aaGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGCACATCAGGCTCa         >  2:4349770/1‑101 (MQ=255)
                                                                        aaGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGCTCa         >  1:4269694/1‑101 (MQ=255)
                                                                        aaGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGCTCa         >  2:299611/1‑101 (MQ=255)
                                                                         ggggggTGCGGGGG.TGTGAAC.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGCTCAg        <  2:3566357/100‑1 (MQ=255)
                                                                         aGGGGGTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGCTCAg        >  2:2396845/1‑101 (MQ=255)
                                                                          gggggTGCGGGGG.TGTGAAC.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGCTCAGc       <  2:48495/101‑1 (MQ=255)
                                                                          gggggTGCGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGCTCAGc       <  1:396193/101‑1 (MQ=255)
                                                                                ggggggg.TTGGAAA.CCCCCGGCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGCTCAGCGtcat  <  1:4366100/100‑1 (MQ=255)
                                                                                ||                                                                                                     
TCGGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAAGGGGGTCGGGGGG.TGTGAAA.CCCCC.GCCGCCGCCGCCGGATCAGCCGCCCATCCCGAACCATTGCGCCCAAAGGCCCGCCGCCCAGAACATCAGGCTCAGCGTCAT  >  NC_014034/530949‑531128

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG