Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	774,622	2 bp→TC	100%	intergenic (‑76/+43)	oppB1 ← / ← oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	774,622	0	G→T	100.0%	23.6	8	intergenic (‑76/+44)	oppB1/oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (7/0):  ref base (0/0):  total (7/1)
*	NC_014034	774,623	0	G→C	100.0%	28.4	8	intergenic (‑77/+43)	oppB1/oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (7/1):  ref base (0/0):  total (7/1)

CCGGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCCGGT.CGA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCG  >  NC_014034/774595‑774705                            ||                                                                                     cgcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACg              >  2:2670421/11‑101 (MQ=255) cgcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACg              >  2:5579645/11‑101 (MQ=255) cgcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGGCTCTTATCCTGCGATCACTTCGCCCCCGGCCCGGCGGCCACg              >  2:2183611/11‑101 (MQ=255)   caggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGtt            >  2:852707/9‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc           >  1:2693192/8‑101 (MQ=255)       cccggcgcCCCCCCGCCCGGGCCGGCCCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAg        <  2:3416060/97‑1 (MQ=255)         cggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTa      >  2:1487733/3‑101 (MQ=255)         cggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGGCGGCCACGTTCCAGTa      >  1:4347640/3‑101 (MQ=255)             gcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCg  >  2:1650388/1‑101 (MQ=255)                            ||                                                                                     CCGGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCCGGT.CGA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCG  >  NC_014034/774595‑774705 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 39 ≤  ATCG