STRAIN: SE8R1B

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breseq  version 0.22  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSE8R1B_1_sequence.txt5,800,386585,838,986100.0%101.0 bases101 bases95.9%
errorsSE8R1B_2_sequence.txt5,800,091585,809,191100.0%101.0 bases101 bases95.0%
total11,600,4771,171,648,177100.0%101.0 bases101 bases95.5%

Reference Sequence Information

seq idlengthfit meanfit dispersiondescription
coveragedistributionNC_0140343,738,958294.33.2Rhodobacter capsulatus SB 1003 chromosome, complete genome.
coveragedistributionNC_014035132,962183.02.1Rhodobacter capsulatus SB 1003 plasmid pRCB133, complete sequence.
total3,871,920

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

Junction Evidence

reference sequencepr(no read start)
NC_0140340.28950
NC_0140350.44609

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Polymorphism E-value cutoff2
Polymorphism frequency cutoffOFF
Polymorphism minimum coverage each strandOFF
Polymorphism homopolymer length cutoffOFF
Polymorphism bias cutoff0
Polymorphism predict indel polymorphismsOFF

Execution Times

stepstartendelapsed
Read and reference sequence file input12:37:30 21 Mar 201312:42:02 21 Mar 20134 minutes 32 seconds
Read alignment to reference genome12:42:03 21 Mar 201313:34:53 21 Mar 201352 minutes 50 seconds
Preprocessing alignments for candidate junction identification13:34:53 21 Mar 201313:44:37 21 Mar 20139 minutes 44 seconds
Preliminary analysis of coverage distribution13:44:37 21 Mar 201313:57:16 21 Mar 201312 minutes 39 seconds
Identifying junction candidates13:57:16 21 Mar 201313:57:27 21 Mar 201311 seconds
Re-alignment to junction candidates13:57:27 21 Mar 201314:10:34 21 Mar 201313 minutes 7 seconds
Resolving alignments with junction candidates14:10:34 21 Mar 201314:25:51 21 Mar 201315 minutes 17 seconds
Creating BAM files14:25:51 21 Mar 201314:37:28 21 Mar 201311 minutes 37 seconds
Tabulating error counts14:37:28 21 Mar 201314:41:40 21 Mar 20134 minutes 12 seconds
Re-calibrating base error rates14:41:40 21 Mar 201314:41:43 21 Mar 20133 seconds
Examining read alignment evidence14:41:43 21 Mar 201315:26:01 21 Mar 201344 minutes 18 seconds
Polymorphism statistics15:26:01 21 Mar 201315:26:16 21 Mar 201315 seconds
Output15:26:16 21 Mar 201315:37:47 21 Mar 201311 minutes 31 seconds
Total 3 hours 16 seconds