Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,301,094	Δ1 bp	100%	intergenic (+51/‑58)	urtA → / → urtC	urea ABC transporter permease UrtA/urea ABC transporter permease UrtC

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,301,094	0	C→.	100.0%	22.3	72	intergenic (+51/‑58)	urtA/urtC	urea ABC transporter permease UrtA/urea ABC transporter permease UrtC
Reads supporting (aligned to +/- strand):  new base (46/18):  ref base (0/8):  total (46/26)
Fisher's exact test strand distribution p-value = 1.31e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.57e-01

CCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGGCG.CCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTCCCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAACCCCTCGGTGCT  >  NC_014034/1300995‑1301189                                                                                                      |                                                                                                 ccgcccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCACAATATCCCGC.GGGGGTc                                                                                                  >  1:2505091/1‑101 (MQ=38) ccgcccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTc                                                                                                  <  2:2042948/101‑1 (MQ=255) ccgcccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTc                                                                                                  <  2:3121841/101‑1 (MQ=255) ccgcccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTc                                                                                                  <  1:2631651/101‑1 (MQ=255)  cgcccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTcc                                                                                                 <  1:1180496/101‑1 (MQ=255)  cgcccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTcc                                                                                                 <  1:1216131/101‑1 (MQ=255)  cgcccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTcc                                                                                                 <  1:3164001/101‑1 (MQ=255)   gcccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTccg                                                                                                <  2:2268884/101‑2 (MQ=255)    cccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTccgg                                                                                               <  2:1820261/101‑3 (MQ=255)    cccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTccgg                                                                                               >  2:1966121/1‑99 (MQ=255)    cccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTccgg                                                                                               <  2:3403200/101‑3 (MQ=255)     ccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTCCGgg                                                                                              <  2:3733595/101‑1 (MQ=37)     ccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTCCGgg                                                                                              <  1:2171823/101‑1 (MQ=37)      cgcgcgGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTCCGggg                                                                                             <  2:1612972/101‑1 (MQ=37)      cgcgcgGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTCCGggg                                                                                             <  2:1941117/101‑1 (MQ=37)      cgcgcgGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTCCGggg                                                                                             <  1:3215391/101‑1 (MQ=37)      cgcgcgGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTCCGggg                                                                                             <  2:2944062/101‑1 (MQ=37)        cgcgGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTCCGggggc                                                                                           <  1:3224575/101‑2 (MQ=37)         gcgGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGgcg                                                                                         >  1:3025628/1‑101 (MQ=25)         ccgGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGgcg                                                                                         <  2:2069296/100‑1 (MQ=25)          cgGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGgcgc                                                                                        <  2:2662265/101‑1 (MQ=25)          cgGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGgcgc                                                                                        <  2:4145544/101‑1 (MQ=25)             catcatCGCGCTGCGCGGCCGTTCCTCCGGATATGGAGCCTATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGaa                                                                                     <  2:1009576/101‑1 (MQ=18)              atcatcGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAg                                                                                    <  2:1269987/101‑1 (MQ=25)              atcatcGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAg                                                                                    <  1:3951820/101‑1 (MQ=25)                catcGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGcc                                                                                  <  2:2473398/101‑1 (MQ=25)                catcGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGACCCCCGCCGCCCTTTCANCTTGCCCAAAATNNNCCTN.CCGNGT.CNGGNNGCNCGATGcc                                                                                  >  1:3833590/1‑101 (MQ=255)                  tcGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTTCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGcccc                                                                                <  2:2897674/101‑1 (MQ=21)                  tcGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGcccc                                                                                <  1:51144/101‑1 (MQ=21)                     cgcTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATCTCCCGC.GGGGGT.CCGGGGCCGCGAAGCCCCC.gg                                                                            >  1:3649171/1‑101 (MQ=21)                     cgcTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.gg                                                                            >  1:2143630/1‑101 (MQ=21)                       cTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.Ggcg                                                                          >  2:3291572/1‑101 (MQ=21)                         gcgcGGCCGCGCCGCGGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGcgcc                                                                        <  2:4159770/101‑1 (MQ=18)                         gcgcGGCCGCGCCGCCGGAGATTGAGCCGATCCCCCCCGCCGCCCTTTCATCTTGCCCAAAATACCCCGG.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGcgcc                                                                        <  1:1898385/101‑1 (MQ=18)                         gcgcCGCCGCGCCGCCGGAGAGTGAGCCGATCCCCCCCGCCGCCCTTTCATCTTGCCCAAAATACCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGcgcc                                                                        <  2:2656938/101‑1 (MQ=17)                           gcgGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGcgccgc                                                                      >  2:1807558/1‑101 (MQ=18)                           gcgGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGcgccgc                                                                      >  2:236584/1‑101 (MQ=18)                             ggCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCa                                                                    >  1:456038/1‑101 (MQ=18)                             ggCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCa                                                                    >  2:3818404/1‑101 (MQ=18)                                  cgccgcCGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATcc                                                               >  2:2618648/1‑101 (MQ=18)                                   gccgccGGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTCCCCAAAATACGCCGC.GGCGGT.CCGGGGGCGCGAAGCCCCC.CGCGCCGCCAGACccc                                                              >  1:3555097/1‑101 (MQ=18)                                        cgGAGATTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGgaga                                                         >  2:3268272/1‑101 (MQ=18)                                            gaTTGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGAcccc                                                     >  2:3538515/1‑101 (MQ=18)                                            gaTTGAGCCGACCCCCCCCCCCC.CCTTTTCCCCTGGCCAAAA.ATCCCCCGGGGGGGGCGGGGGGCGCGACGCCCCC.GGCGCCGCCAGATCCCGGAGAcccc                                                     <  1:1371521/101‑1 (MQ=255)                                             attgccccGCTGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGTGT.CCGGGGGCGTGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCg                                                    <  2:2106927/95‑1 (MQ=17)                                              ttGAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGTGCGGCCAGATCACGGAGACCCCgg                                                   >  2:2389124/1‑100 (MQ=18)                                                gAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATg                                                 >  2:1197086/1‑101 (MQ=18)                                                gAGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATg                                                 <  1:1903172/101‑1 (MQ=18)                                                gAGCCGAGGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATg                                                 <  2:2796889/101‑1 (MQ=18)                                                 aGCCGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGa                                                >  1:3948614/1‑101 (MQ=18)                                                    cGATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGacac                                             >  2:3297222/1‑101 (MQ=18)                                                     gATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGAcaca                                            >  2:1869097/1‑101 (MQ=18)                                                     gATGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGAcaca                                            >  1:3058778/1‑101 (MQ=18)                                                      aTGCCCCCCGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAg                                           <  2:3486536/101‑1 (MQ=18)                                                           ccccGCCGCCCTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGAt                                      <  1:2542402/101‑1 (MQ=18)                                                              ccgccccttttCATCTTGCCCAAAATCTCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGAc                                   <  2:2493398/93‑1 (MQ=21)                                                               gccgcccTTTCATCTTGCCCAAAATATCCCGG.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGAcc                                  <  1:1960693/96‑1 (MQ=21)                                                                  gcccTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCaa                               >  2:4088021/3‑101 (MQ=255)                                                                  ccccTTTCATCTTCCCAAAAATACCCCG..GGGGGGCCGGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCaa                               <  1:2669605/99‑1 (MQ=25)                                                                   cccTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAg                              >  1:1165839/2‑101 (MQ=255)                                                                   cccTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAg                              >  1:1420955/2‑101 (MQ=255)                                                                    ccTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGAAGatcggaag                             >  1:3321831/1‑94 (MQ=21)                                                                     ttttCTTCTTCCCCAAAATCCCCCG..GGGGGGCCGGGGGGCGCGAACCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAggg                            <  1:1993266/100‑1 (MQ=255)                                                                     cTTTCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAggg                            >  1:2805514/1‑101 (MQ=255)                                                                      tttCTTCTTGCCCAAAATACCCCG..GGGGGGGCCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGt                           <  1:678876/101‑1 (MQ=25)                                                                      tttCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGt                           >  2:73896/1‑101 (MQ=255)                                                                      tttCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGt                           >  1:1171194/1‑101 (MQ=255)                                                                       ttCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGtt                          >  1:957374/1‑101 (MQ=255)                                                                       ttCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGtt                          >  1:2632618/1‑101 (MQ=255)                                                                       ttCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGtt                          >  2:2518471/1‑101 (MQ=255)                                                                       ttCATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGtt                          >  1:1624931/1‑101 (MQ=255)                                                                        ttcctcttcccAAAAACCCCCGG.GGGGGGCCGGGGGGCGCGACCCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGtt                          <  1:3845442/93‑1 (MQ=255)                                                                         cATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTcc                        >  1:746514/1‑101 (MQ=255)                                                                         cATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTcc                        >  1:3428694/1‑101 (MQ=255)                                                                         cATCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTcc                        >  2:178199/1‑101 (MQ=255)                                                                         cATCTCCCCAAAAATCCCCCGG.GGGGGT.CGGGGGGCGCGACCCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTcc                        <  1:1869217/101‑1 (MQ=255)                                                                          aTCTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACCGCCGATGACCCAAGGGGTCCt                       >  2:3176869/1‑101 (MQ=255)                                                                            ccgccccAAAAGCCCCCGG.GGGGGGCGGGGGGGCCCGACCCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTg                      <  2:2143485/97‑1 (MQ=255)                                                                            cTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGt                     >  1:2946241/1‑101 (MQ=255)                                                                            cTTGCCCAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGt                     >  1:596899/1‑101 (MQ=255)                                                                                 ccAAAATATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCaaa                >  1:703014/1‑101 (MQ=255)                                                                                 ccAAAAACTCCCGG.GGGGGGCCGGGGGGC.CCAAGCCCCCGGGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCaa                 <  1:1745707/101‑1 (MQ=255)                                                                                    aaaTATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAAc             >  2:3882070/1‑101 (MQ=255)                                                                                    aaaTATCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAAc             >  1:272428/1‑101 (MQ=255)                                                                                        ctCCCGC.GGGGGT.CGGGGGGCGGGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAACCCCt         <  2:3329149/100‑1 (MQ=38)                                                                                        atCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAACCCCt         >  1:1107978/1‑101 (MQ=255)                                                                                         tCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAACCCCTc        >  1:3318519/1‑101 (MQ=255)                                                                                         tCCCGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAACCCCTc        >  1:2276404/1‑101 (MQ=255)                                                                                         tCCCGC.GGCGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGCTGAGCCAAGGGTTTGTGTGCAAAAAACCCTc        >  2:646442/1‑101 (MQ=25)                                                                                         ccccGC.GGGGG.CCCGGGGGCGGGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAACCCCTc        <  2:1833564/100‑1 (MQ=255)                                                                                          cccGG.GGGGGGCCGGGGGGCCCAAGCCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAACCCCTc        <  1:1725632/101‑1 (MQ=255)                                                                                          cccGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAACCCCTCg       >  1:1347225/1‑101 (MQ=255)                                                                                           ccGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAACCCCTCgg      >  2:2294673/1‑101 (MQ=255)                                                                                           ccGC.GGGGGT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAACCCCTCgg      >  2:1127487/1‑101 (MQ=255)                                                                                                gggggT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAACCCCTCGGTGCt  >  2:2747489/1‑101 (MQ=255)                                                                                                gggggT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAACCCCTCGGTGCt  <  2:1308111/101‑1 (MQ=255)                                                                                                gggggT.CCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAACCCCTCGGTGCt  >  2:1025886/1‑101 (MQ=255)                                                                                                 ggggTCCCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAACCCCTCGGTGCt  <  2:702937/101‑1 (MQ=255)                                                                                                      |                                                                                                 CCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGGCG.CCTTTCATCTTGCCCAAAATATCCCGC.GGGGGTCCCGGGGGCGCGAAGCCCCC.GGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGATGACCCAAGGGTTCCTGTCCAAAAACCCCTCGGTGCT  >  NC_014034/1300995‑1301189 Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG