BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,484	Δ1 bp	19.7%	intergenic (+296/‑635)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,484	0	G→.	19.7%	158.9 ‑0.7 ‑0.0	239	intergenic (+296/‑635)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (18/29): ref base (85/107): total (103/136)
Fisher's exact test strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.56e-01

Only 100 of 257 total aligned reads displayed.


CGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGG  >  NC_014034/1395384‑1395584


cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCg                                                                                                      >  2:3457057/1‑101 (MQ=255)


 gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGa                                                                                                     >  2:848418/1‑101 (MQ=255)


 gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGa                                                                                                     >  1:544229/1‑101 (MQ=255)


  tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGAc                                                                                                    >  2:2183707/1‑101 (MQ=255)


   ggATCGAAACTGCACCCGCTGGCCCTGCCTGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACa                                                                                                   >  1:2624157/1‑101 (MQ=255)


   ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACa                                                                                                   >  2:3219197/1‑101 (MQ=255)


   ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACa                                                                                                   >  1:2443341/1‑101 (MQ=255)


       cGAACCTGCACCCGCTGGCCCTGCCGGTGAACGGTGATCAGCGGTCCCGCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCt                                                                                               <  2:3503070/101‑1 (MQ=38)


        gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTg                                                                                              <  1:848362/101‑1 (MQ=255)


           aCTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTa                                                                                           <  1:814588/101‑1 (MQ=255)


           aCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTa                                                                                           <  2:4210035/101‑1 (MQ=255)


             tGCCCCCGCTGCCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAAc                                                                                         <  1:4159381/101‑1 (MQ=255)


             tGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAAc                                                                                         <  1:3913955/101‑1 (MQ=255)


              gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACg                                                                                        <  2:2730245/101‑1 (MQ=255)


               cACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACgt                                                                                       >  1:1655428/1‑101 (MQ=255)


                aCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGGAACgtg                                                                                      >  1:587742/1‑101 (MQ=255)


                aCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGGAACgtg                                                                                      >  2:3057989/1‑101 (MQ=255)


                  ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTc                                                                                    <  1:2726905/101‑1 (MQ=255)


                  ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTc                                                                                    >  2:3356045/1‑101 (MQ=255)


                   cGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCg                                                                                   <  1:1212377/101‑1 (MQ=255)


                    gCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGGAACGTGTCGc                                                                                  >  2:3001589/1‑101 (MQ=255)


                      tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                                                                                >  2:3625845/1‑101 (MQ=255)


                       ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGNTGGTAACGTGTCGCTcc                                                                               >  2:1049215/1‑101 (MQ=255)


                       ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                               <  2:2875529/101‑1 (MQ=255)


                       ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CG.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                               >  1:3676267/1‑101 (MQ=255)


                         cccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc                                                                             >  2:4022521/1‑101 (MQ=255)


                          ccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTccccc                                                                            <  1:100034/101‑1 (MQ=255)


                           cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                           <  2:52103/101‑1 (MQ=255)


                             gCCCGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgc                                                                         <  2:4167790/101‑1 (MQ=255)


                              ccGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcccg                                                                        >  1:3139498/1‑101 (MQ=255)


                                   gatcggtgatcAGCGGTCGCTCCCCCCGCGTGGGCGTGGATCGAAACATCGCCG...CT.TTGGCAGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGTGc                                                                   <  2:3481269/101‑1 (MQ=255)


                                   gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                                   >  2:1330952/1‑101 (MQ=255)


                                   gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                                   <  2:1922950/101‑1 (MQ=255)


                                   gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                                   <  2:971995/101‑1 (MQ=255)


                                    atcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCg                                                                  <  1:2183852/101‑1 (MQ=255)


                                      gggtggtgAGGGGCCGCTCCGCCCGCGGGGGCGGGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg                                                                <  2:1188078/100‑1 (MQ=38)


                                      cggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg                                                                >  2:3937025/1‑101 (MQ=255)


                                      cggtgatcAGCGGACGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg                                                                <  2:3089563/101‑1 (MQ=255)


                                       ggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg                                                               >  1:1832167/1‑101 (MQ=255)


                                       ggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg                                                               <  2:3912547/101‑1 (MQ=255)


                                        gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                                              >  2:3691073/1‑101 (MQ=255)


                                        gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                                              <  2:4127914/101‑1 (MQ=255)


                                        gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                                              >  1:107331/1‑101 (MQ=255)


                                        gctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             <  2:2052707/92‑1 (MQ=2)


                                         tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             >  2:1592116/1‑101 (MQ=255)


                                         tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             <  1:7210/101‑1 (MQ=255)


                                         ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            >  1:1539092/9‑101 (MQ=0)


                                          tggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           <  1:3782888/94‑1 (MQ=2)


                                           atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           <  1:3876146/101‑1 (MQ=255)


                                             cAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         <  1:4228421/101‑1 (MQ=255)


                                             cAGCGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         <  2:271902/101‑1 (MQ=255)


                                              cgtggTCGCTCCCCCCGCGGGGGGGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       >  1:398216/4‑101 (MQ=2)


                                              cgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       >  2:599069/4‑101 (MQ=2)


                                              aGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                        <  1:1283247/101‑1 (MQ=255)


                                              aGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTAGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                        >  2:579190/1‑101 (MQ=255)


                                               gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                      >  1:4108360/3‑101 (MQ=2)


                                               gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                      <  1:2609604/99‑1 (MQ=2)


                                               gCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       <  2:2941449/101‑1 (MQ=255)


                                               gCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       >  2:1731691/1‑101 (MQ=255)


                                                cGGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                      <  1:3367919/101‑1 (MQ=255)


                                                 ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                                    >  1:3080607/1‑99 (MQ=2)


                                                 ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                                    <  2:1836845/101‑3 (MQ=2)


                                                 ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                                    <  1:3625243/101‑3 (MQ=2)


                                                 ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                     <  2:2443157/101‑1 (MQ=255)


                                                    ngccncccccGCGGGGGCGTGGATCGAAAC.TCCCCGAACCTGTCGGCGG.CGA.ACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtcg                                                   <  1:3196898/96‑2 (MQ=0)


                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                                  <  1:2853850/101‑1 (MQ=255)


                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                                  <  1:3457309/101‑1 (MQ=255)


                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                                >  1:1979495/1‑98 (MQ=2)


                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                                <  1:993780/101‑4 (MQ=2)


                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACAgctttca                                               <  1:2399969/101‑5 (MQ=2)


                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                               <  1:1471450/101‑5 (MQ=2)


                                                       tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                                               >  1:2066742/1‑101 (MQ=255)


                                                       tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                                               >  1:2359869/1‑101 (MQ=255)


                                                        ccccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                                             <  1:1767511/101‑7 (MQ=0)


                                                             cgcgGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTc                                         <  1:3219436/101‑1 (MQ=255)


                                                             cgcgGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTc                                         >  1:554238/1‑101 (MQ=255)


                                                               cgGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGc                                       <  1:158392/101‑1 (MQ=255)


                                                                 ggggCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGGCACTc                                     >  2:1153172/1‑101 (MQ=255)


                                                                   ggCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGt                                   <  2:2075301/101‑1 (MQ=255)


                                                                    gCGTTGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTc                                  <  2:554174/101‑1 (MQ=255)


                                                                    gCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTc                                  <  2:3404148/101‑1 (MQ=255)


                                                                      gTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                                <  2:3829519/101‑1 (MQ=255)


                                                                      gTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                                >  1:1936922/1‑101 (MQ=255)


                                                                      gTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                                <  2:2359693/101‑1 (MQ=255)


                                                                       tGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAg                               <  1:1341215/101‑1 (MQ=255)


                                                                         gATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGcc                             <  2:2483989/101‑1 (MQ=255)


                                                                          aTCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCg                            <  2:1655313/101‑1 (MQ=255)


                                                                              aagcATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCt                        >  1:2418477/4‑101 (MQ=255)


                                                                               aaCATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTg                       <  1:2257267/101‑1 (MQ=255)


                                                                                 cATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCt                     >  2:2007715/1‑101 (MQ=255)


                                                                                  aTCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc                    <  2:3556099/101‑1 (MQ=255)


                                                                                  aTCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc                    <  2:1567323/101‑1 (MQ=255)


                                                                                   tCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcg                   <  1:2916134/101‑1 (MQ=255)


                                                                                    cgccg...cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgt                  >  1:2983715/1‑101 (MQ=255)


                                                                                             t.ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcc            >  2:1557093/1‑101 (MQ=255)


                                                                                                 ggCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTccccc         >  1:4171262/1‑101 (MQ=255)


                                                                                                   cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCcg       >  1:3543589/1‑101 (MQ=255)


                                                                                                   cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCcg       <  1:3740798/101‑1 (MQ=255)


                                                                                                       cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGGTCGTCGCTTCCCCCGCggg   >  2:3205056/1‑101 (MQ=255)


                                                                                                       cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCggg   >  2:765501/1‑101 (MQ=255)


CGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGG  >  NC_014034/1395384‑1395584

Base quality scores:


ATCG

< 3 ≤


ATCG

< 19 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG