Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,546 Δ1 bp 10.9% intergenic (+358/‑573) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5460G→.10.9% 100.7 0.0 ‑0.9305intergenic (+358/‑573)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/17):  ref base (119/124):  total (147/158)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.33e-01

Only 100 of 322 total aligned reads displayed.

CGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGC  >  NC_014034/1395447‑1395646
                                                                                                   |                                                                                                      
cgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGc                                                                                                       <  1:158392/101‑1 (MQ=255)
     gCGTTGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tc                                                                                                 <  2:554174/101‑1 (MQ=255)
     gCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tc                                                                                                 <  2:3404148/101‑1 (MQ=255)
      cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                                <  1:3754861/101‑1 (MQ=255)
       gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                               <  2:3829519/101‑1 (MQ=255)
       gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                               >  1:1936922/1‑101 (MQ=255)
       gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                               <  2:2359693/101‑1 (MQ=255)
         ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGc                                                                                             >  2:1836906/1‑101 (MQ=255)
          gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGcc                                                                                            <  2:2483989/101‑1 (MQ=255)
           aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCg                                                                                           <  2:1655313/101‑1 (MQ=255)
             cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCCG.c                                                                                        <  1:3128442/101‑1 (MQ=255)
               aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCt                                                                                      <  2:2438293/101‑1 (MQ=255)
                  cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCt                                                                                   >  2:2007715/1‑101 (MQ=255)
                   aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCtc                                                                                  <  2:1567323/101‑1 (MQ=255)
                   aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCtc                                                                                  >  1:178262/1‑101 (MQ=255)
                   aTCGCCGCTTTGGCGGTCGACAGCCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCtc                                                                                  <  2:544165/101‑1 (MQ=255)
                          cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTc                                                                           <  1:3725765/101‑1 (MQ=255)
                             tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTcccc                                                                        >  1:2098719/1‑101 (MQ=255)
                              ggCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTccccc                                                                       >  1:4171262/1‑101 (MQ=255)
                               gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTcccccc                                                                      <  2:1162012/101‑1 (MQ=255)
                                     gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCgggg                                                                <  1:2018102/101‑1 (MQ=255)
                                     gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCgggg                                                                <  2:2151676/101‑1 (MQ=255)
                                     gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCgggg                                                                <  2:3638415/101‑1 (MQ=255)
                                      aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCggggg                                                               >  2:2081737/1‑101 (MQ=255)
                                       cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGc                                                              >  2:1083280/1‑101 (MQ=255)
                                       cAGGCTGGTAACGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGc                                                              <  1:3058214/101‑1 (MQ=255)
                                           gccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTgg                                                          <  1:599133/91‑1 (MQ=0)
                                           aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTgg                                                          >  2:459927/11‑101 (MQ=2)
                                           aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTgg                                                          >  1:1035369/11‑101 (MQ=2)
                                            aggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGa                                                         >  1:3435224/10‑101 (MQ=0)
                                             ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  2:1984778/93‑1 (MQ=2)
                                             ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        >  1:3540112/9‑101 (MQ=2)
                                             ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        >  1:391596/1‑101 (MQ=255)
                                             cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  2:404372/93‑1 (MQ=2)
                                               tAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      >  1:440169/1‑101 (MQ=255)
                                                cgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                     <  2:1713020/96‑1 (MQ=2)
                                                aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                     >  1:1096869/1‑101 (MQ=255)
                                                aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                     <  2:3345418/101‑1 (MQ=255)
                                                aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGa                                                     >  1:1819046/1‑101 (MQ=255)
                                                 gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGGGGGGGTCgga                                                    >  1:2962384/5‑99 (MQ=2)
                                                 gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    >  1:1743777/5‑101 (MQ=2)
                                                  gtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   >  1:2473691/4‑101 (MQ=2)
                                                  ctggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGACGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   >  2:3982834/4‑101 (MQ=255)
                                                   gtgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                  <  1:3001814/101‑1 (MQ=255)
                                                   acgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAac                                                  >  2:721106/3‑101 (MQ=2)
                                                    ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                 <  2:961627/100‑1 (MQ=2)
                                                    ggtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAACCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                 <  1:1378143/100‑1 (MQ=2)
                                                     ggcGCGCCCCCCGGGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccc                                                <  2:1806895/99‑2 (MQ=0)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTCGATCGAAaccac                                               <  1:2293636/101‑1 (MQ=255)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                               <  1:360973/101‑3 (MQ=2)
                                                      gcGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                               <  1:276813/100‑3 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                              >  1:543445/1‑98 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                              <  2:1341585/101‑4 (MQ=2)
                                                       cGCGCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                              <  2:2554911/101‑5 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                             >  2:899049/1‑97 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgc                                             >  1:4004352/1‑96 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                            <  2:4126783/101‑1 (MQ=255)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                            >  1:323421/1‑101 (MQ=255)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCCCGGGGGCGGGGGTCGAAaccgcgtg                                            >  1:764575/1‑96 (MQ=0)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                            >  1:3318772/1‑95 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgcg                                            >  2:1362953/1‑95 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                           <  1:1225729/101‑8 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                           <  2:1035278/101‑8 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                          <  1:2845386/101‑8 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                          >  2:3931076/1‑94 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                          >  1:3345775/1‑93 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                          >  1:2602771/1‑93 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                          >  1:896318/1‑93 (MQ=2)
                                                            cccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                                         <  2:2618532/101‑10 (MQ=2)
                                                             ccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcgt                                        <  1:1961815/101‑10 (MQ=2)
                                                             ccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcgg                                        >  2:580161/1‑92 (MQ=2)
                                                             ccccGCGGGGGCGTGGAGCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgcg                                        <  2:303094/101‑11 (MQ=0)
                                                              cccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCACCTTGCggg                                       >  2:276987/1‑100 (MQ=255)
                                                                cgcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                     <  2:3601743/101‑1 (MQ=255)
                                                                   gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                  >  2:2415076/1‑101 (MQ=255)
                                                                   gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTGGCGGATTGTc                                  >  1:965997/1‑101 (MQ=255)
                                                                     gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                                >  2:3496251/1‑101 (MQ=255)
                                                                       gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                              <  2:3092441/101‑1 (MQ=255)
                                                                        cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                             >  1:1354653/1‑101 (MQ=255)
                                                                         gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                            <  2:2291987/101‑1 (MQ=255)
                                                                         gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                            <  1:117110/101‑1 (MQ=255)
                                                                          tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                           >  2:2885313/1‑101 (MQ=255)
                                                                          tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                           <  2:4171000/101‑1 (MQ=255)
                                                                           ggATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcag                          >  1:3017568/1‑99 (MQ=255)
                                                                              tCGAAGCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCggg                       <  2:388774/101‑1 (MQ=255)
                                                                              tCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCggg                       <  2:830975/101‑1 (MQ=255)
                                                                                gAAACATCTCGGTGCCGTCGCTCG.TCAAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                     >  2:627097/1‑101 (MQ=255)
                                                                                 aaaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcgagatc                    >  2:177818/1‑96 (MQ=255)
                                                                                 aaaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                    >  1:2714609/1‑101 (MQ=255)
                                                                                 aaaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGACg                    <  1:2415252/101‑1 (MQ=255)
                                                                                    cATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                 >  2:3193785/1‑101 (MQ=255)
                                                                                      tctcGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg               >  1:1151060/1‑101 (MQ=255)
                                                                                            tGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTc         >  2:344238/1‑101 (MQ=255)
                                                                                             gCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcc        >  1:3389267/1‑101 (MQ=255)
                                                                                                gTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccccc     >  2:3186163/1‑101 (MQ=255)
                                                                                                 tCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc    <  1:3813103/101‑1 (MQ=255)
                                                                                                 tCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc    <  2:3247176/101‑1 (MQ=255)
                                                                                                 tCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc    <  1:3678315/101‑1 (MQ=255)
                                                                                                  cGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcg   >  2:1494075/1‑101 (MQ=255)
                                                                                                   gCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgc  >  1:2142181/1‑101 (MQ=255)
                                                                                                   |                                                                                                      
CGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGC  >  NC_014034/1395447‑1395646

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG