BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,698	Δ1 bp	6.1%	intergenic (+510/‑421)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,698	0	A→.	6.1%	28.7 ‑0.3 ‑0.0	198	intergenic (+510/‑421)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (7/5): ref base (98/87): total (105/93)
Fisher's exact test strand distribution p-value = 7.74e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.77e-01

Only 100 of 217 total aligned reads displayed.


CCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTG  >  NC_014034/1395598‑1395798


ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.Ca                                                                                                         >  2:3412179/1‑101 (MQ=255)


  accTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGt                                                                                                       <  2:440112/101‑1 (MQ=255)


   ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTc                                                                                                      <  2:1794268/101‑1 (MQ=255)


   ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTc                                                                                                      <  1:1691289/101‑1 (MQ=255)


    cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCg                                                                                                     <  2:877191/101‑1 (MQ=255)


      tGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCt                                                                                                   >  1:893133/1‑101 (MQ=255)


      ggCGGATTGCCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCt                                                                                                   <  2:3358922/100‑1 (MQ=255)


       ggggATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTc                                                                                                  <  2:660294/99‑1 (MQ=255)


        cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTcc                                                                                                 >  1:1327968/1‑101 (MQ=255)


        cGGATTCTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCATCTGCATGTAGTCGG.CG.G.GCAGTTCGC.GCCG.CAGTCGCTcc                                                                                                 >  2:578748/1‑101 (MQ=255)


           aTTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTccccc                                                                                              >  1:591557/1‑101 (MQ=255)


           aTTGTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTccccc                                                                                              <  2:3389022/101‑1 (MQ=255)


            ttGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                                             <  1:2132095/101‑1 (MQ=255)


             tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCcg                                                                                            >  2:2614954/1‑101 (MQ=255)


               tCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCcgcg                                                                                          <  2:2943881/101‑1 (MQ=255)


                   tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCggggg                                                                                      >  2:429380/1‑101 (MQ=255)


                    ttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGc                                                                                     <  2:3874758/101‑1 (MQ=255)


                        ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG.GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                                                  >  2:3429367/10‑101 (MQ=2)


                        ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG.GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                                                  >  2:3624233/10‑101 (MQ=2)


                        ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG.GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                                                  >  1:3257903/10‑101 (MQ=2)


                        gggggCGAGGCGGCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTgg                                                                                 <  2:1832047/100‑1 (MQ=255)


                         ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                                >  2:3654634/1‑101 (MQ=255)


                         gatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCCTGACCGCAT.CGAA.GCTGGTCAC.ATCG.CCGTCGCTCCCCCCGCGGGGGCGTgg                                                                                 <  2:2197809/91‑1 (MQ=2)


                          gggCGAAGCGTCGCCCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                               <  1:765578/101‑1 (MQ=255)


                           ggCGAAGCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGGATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                              <  1:2794140/101‑1 (MQ=255)


                           gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG.GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                               <  2:2750288/95‑1 (MQ=2)


                           gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG.GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                               <  1:3901565/95‑1 (MQ=2)


                              gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                           >  1:1270206/1‑101 (MQ=255)


                              gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                           >  2:22398/1‑101 (MQ=255)


                               catcGTCGCTCCCCCCGCGGGGACGTGGATCGAATCCTATGAGGGCTGGCGGCCC.G.GTCGTTGAT.CCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                            <  2:3165394/98‑1 (MQ=0)


                                agggTCGCCCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                         <  2:853172/98‑1 (MQ=255)


                                aGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                         >  1:4028785/1‑101 (MQ=255)


                                 gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                        >  1:2687011/1‑101 (MQ=255)


                                  ggccGCGCCCCCGGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.g                                                                      <  1:598615/98‑1 (MQ=37)


                                  cggcGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.g                                                                      <  1:3325631/98‑1 (MQ=255)


                                  cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG.GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaa                                                                        >  2:3298710/1‑99 (MQ=2)


                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.gg                                                                     >  2:3709900/1‑101 (MQ=255)


                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGt                                                                    <  1:2778662/101‑1 (MQ=255)


                                        tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATgg                                                                >  2:1603454/1‑101 (MQ=255)


                                        tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATgg                                                                >  2:1172760/1‑101 (MQ=255)


                                        cccccccGGGGGGGGGGGGATCAAAACACCTGCTTGTAGTGGG.CG.G.GCGGTCGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATgg                                                                <  1:3873470/100‑1 (MQ=21)


                                            cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCt                                                            <  2:3153209/101‑1 (MQ=255)


                                              cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTgg                                                          <  2:2499199/101‑1 (MQ=255)


                                              cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTgg                                                          >  1:2612570/1‑101 (MQ=255)


                                               ggggggcCGTGAATCGAAACATCTGGTTGTGGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGgc                                                         <  2:1048267/99‑1 (MQ=37)


                                               gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGgc                                                         >  1:1403156/1‑101 (MQ=255)


                                                 gggggCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCT.CAGTCGATTCCCCCGCGGGGGCGTGGATCGAAACT.GTTATGGATGTgactc                                                       >  2:2072258/1‑97 (MQ=37)


                                                   gggCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCaa                                                     >  1:3001028/1‑101 (MQ=255)


                                                     gCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAAc                                                   >  1:2737661/1‑101 (MQ=255)


                                                      cGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACg                                                  >  1:1783396/1‑101 (MQ=255)


                                                       gTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACgg                                                 >  1:640301/1‑101 (MQ=255)


                                                       gTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACgg                                                 <  1:3185690/101‑1 (MQ=255)


                                                        tGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..a                                              >  1:3518232/1‑101 (MQ=255)


                                                        gggATCGAACCATCTGCACGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..a                                              <  2:1913152/100‑1 (MQ=255)


                                                          gATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..aaa                                            <  2:3930694/101‑1 (MQ=255)


                                                          gATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..aaa                                            >  2:616699/1‑101 (MQ=255)


                                                          gATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAT.GGTATCGGTCTGGCGCAAACGG..aag                                            >  2:3092418/1‑100 (MQ=255)


                                                             cGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTc                                         >  1:964882/1‑101 (MQ=255)


                                                              gAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCt                                        >  1:2364363/1‑101 (MQ=255)


                                                              gAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCt                                        <  2:2609382/101‑1 (MQ=255)


                                                                  cATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGCCTgtcg                                    >  2:3220016/1‑101 (MQ=255)


                                                                    tCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTgtcgtc                                  >  2:1643354/1‑101 (MQ=255)


                                                                     cTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGtcgtcg                                 <  1:4113891/101‑1 (MQ=255)


                                                                     cTGCATGTAGGGGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGtcgtcg                                 <  1:2951518/101‑1 (MQ=255)


                                                                       gCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCt                               >  2:1465308/1‑101 (MQ=255)


                                                                        cATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTc                              <  1:443112/101‑1 (MQ=255)


                                                                         aTGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTcc                             >  2:1290095/1‑101 (MQ=255)


                                                                           ttggTCGG.CG.G.CAAGTGGGA.GCGG.CAGTCGCTCCCCCCGCCGGGGCGGGGATCTAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTcccc                           <  2:698051/98‑1 (MQ=25)


                                                                              gTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCcg                        >  1:1802356/1‑101 (MQ=255)


                                                                              gTCGG.CG.G.GCAGTCCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCcg                        <  2:4030918/101‑1 (MQ=255)


                                                                                cgg.cg.g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCcgcg                      >  1:2390898/1‑101 (MQ=255)


                                                                                 gg.cg.g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCgg                     >  2:303445/1‑101 (MQ=255)


                                                                                  g.cg.g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCggg                    >  1:3469124/1‑101 (MQ=255)


                                                                                     g.g.gCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCggggg                  <  1:4122783/101‑1 (MQ=255)


                                                                                       g.gCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGc                 <  2:1327868/101‑1 (MQ=255)


                                                                                          cAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GTTATTGATCTGGCGCAAACGG..AAAGTCTGCCGTCGCTCCCCCCGCGGGGGCGt               >  2:536582/1‑101 (MQ=255)


                                                                                           aGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTg              <  1:1015688/101‑1 (MQ=255)


                                                                                             ttCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGa            >  1:1496272/1‑101 (MQ=255)


                                                                                             ttCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGa            >  2:2350300/1‑101 (MQ=255)


                                                                                               cGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATc          >  1:1820771/1‑101 (MQ=255)


                                                                                               cGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATc          <  1:511539/101‑1 (MQ=255)


                                                                                               cGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATc          >  2:1885877/1‑101 (MQ=255)


                                                                                                tcaagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCCGGTGGTCGCTCCCCCCGCGGGGGCGTg              >  1:3764702/9‑101 (MQ=2)


                                                                                                gA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg         >  1:982636/1‑101 (MQ=255)


                                                                                                 a.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa        <  1:2959564/101‑1 (MQ=255)


                                                                                                   gCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa       >  1:3583692/1‑101 (MQ=255)


                                                                                                    gattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCCGGTGGTCGCTCCCCCCGCGGGGGCGTGGATc          >  2:4109434/5‑101 (MQ=2)


                                                                                                     attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCCGGTGGTCGCTCCCCCCGCGGGGGCGTGGATCg         >  1:1998566/4‑101 (MQ=2)


                                                                                                     attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCCGGTGGTCGCTCCCCCCGCGGGGGCGTGGATCg         >  1:2833475/4‑101 (MQ=2)


                                                                                                      ttcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCCGGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGa        <  1:2451083/99‑1 (MQ=2)


                                                                                                      g.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc    <  1:3056906/101‑1 (MQ=255)


                                                                                                      g.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc    >  1:3110838/1‑101 (MQ=255)


                                                                                                       tcAGTCCCGCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCGGGGTAAGGCCGGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa       <  2:1266001/100‑1 (MQ=2)


                                                                                                        cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCCGGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa      <  1:2056282/101‑1 (MQ=2)


                                                                                                        cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCCGGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa      <  1:1148354/101‑1 (MQ=2)


                                                                                                        cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt   <  1:3412424/101‑1 (MQ=255)


                                                                                                        cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt   >  1:1129484/1‑101 (MQ=255)


                                                                                                        cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt   <  2:3550303/101‑1 (MQ=255)


                                                                                                         aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCCGGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc     >  2:2128267/1‑101 (MQ=2)


                                                                                                         aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTg  >  1:1573329/1‑101 (MQ=255)


CCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTGGCGCAAACGG..AAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTG  >  NC_014034/1395598‑1395798

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 39 ≤


ATCG