Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,831 Δ1 bp 14.7% coding (713/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8310C→.14.7% 107.5 ‑0.2 ‑0.0231coding (713/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/18):  ref base (97/100):  total (113/118)
Fisher's exact test strand distribution p-value = 8.54e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.87e-01

Only 100 of 247 total aligned reads displayed.

TCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1396731‑1396931
                                                                                                       |                                                                                                     
tCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGc                                                                                                       <  1:1571517/101‑1 (MQ=255)
tCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGc                                                                                                       <  1:81719/101‑1 (MQ=255)
 cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCt                                                                                                      >  2:351542/1‑101 (MQ=255)
  gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCtt                                                                                                     <  1:3001409/101‑1 (MQ=255)
    aaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.gg                                                                                                  >  1:538282/1‑101 (MQ=255)
     aCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.ggg                                                                                                 >  2:3730648/1‑101 (MQ=255)
      cccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGt                                                                                                <  2:466183/101‑1 (MQ=255)
       ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTc                                                                                               >  2:694197/1‑101 (MQ=255)
         tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGCCGGCTT.GGGTCGc                                                                                             >  1:1761680/1‑101 (MQ=255)
         tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGc                                                                                             >  1:827461/1‑101 (MQ=255)
         tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGc                                                                                             <  2:3654265/101‑1 (MQ=255)
           tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTc                                                                                           >  1:2002340/1‑101 (MQ=255)
           tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTc                                                                                           >  2:1864479/1‑101 (MQ=255)
            cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTcc                                                                                          <  2:326463/101‑1 (MQ=255)
              cTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTcccc                                                                                        <  2:1515025/101‑1 (MQ=255)
               tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTccccc                                                                                       >  2:2547163/1‑101 (MQ=255)
                 atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCcg                                                                                     <  1:4221290/101‑1 (MQ=255)
                   atGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCcgcg                                                                                   >  2:2725756/1‑101 (MQ=255)
                     gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCggg                                                                                 >  1:436154/1‑101 (MQ=255)
                     gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCggg                                                                                 >  1:3391895/1‑101 (MQ=255)
                      ctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCgggg                                                                                <  2:920898/101‑1 (MQ=255)
                        gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGc                                                                              <  2:1566451/101‑1 (MQ=255)
                        gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGc                                                                              <  1:510028/101‑1 (MQ=255)
                          cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCgg                                                                            >  1:3572571/1‑100 (MQ=255)
                          cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGt                                                                            <  2:1634622/101‑1 (MQ=255)
                            cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTgg                                                                          >  2:1071239/1‑101 (MQ=255)
                            cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  1:1853852/101‑1 (MQ=255)
                            cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGGgg                                                                          >  1:2260516/1‑101 (MQ=255)
                             gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCcgcgagatcggaag                                                                         >  1:3509952/1‑91 (MQ=255)
                             gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGGGTGGa                                                                         >  1:1058055/1‑101 (MQ=255)
                             gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         <  2:22418/101‑1 (MQ=255)
                             gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         >  2:1959849/1‑101 (MQ=255)
                             gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         >  2:3357352/1‑101 (MQ=255)
                              aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  1:1095441/101‑1 (MQ=255)
                               cccatcgccgTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         <  1:2459914/92‑1 (MQ=0)
                                gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGCTCCAAACCGCCTGGGT...GATTATGCACCCCGGGCGGCGT.GGTCGGCTGCCCCCGCcggggggcgggagg                                                                      >  2:3295838/1‑92 (MQ=12)
                                 catcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       >  1:4200071/8‑101 (MQ=2)
                                  ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  1:1071333/101‑1 (MQ=255)
                                  ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  1:2368617/101‑1 (MQ=255)
                                    ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAc                                                                  >  1:1293777/1‑101 (MQ=255)
                                    ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCACGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAc                                                                  >  2:3621235/1‑101 (MQ=255)
                                    cgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  1:3474012/97‑1 (MQ=2)
                                     gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   >  1:3178015/4‑101 (MQ=2)
                                      ggggcccTCCCCCCGGGGGGGCGTGGATCGAAACCGGGTGGAT...GATCATGCAGCGCGGACGGCTTGGGGCCCCCCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                 <  2:3752885/95‑1 (MQ=25)
                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                                >  2:1485737/1‑99 (MQ=2)
                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                                <  2:2561632/101‑3 (MQ=2)
                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                                               >  1:1428498/1‑98 (MQ=2)
                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                                               <  1:3966214/101‑4 (MQ=2)
                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTc                                                             >  1:1847200/1‑101 (MQ=255)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatgg                                                              <  2:1424592/101‑5 (MQ=2)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggc                                                             >  1:1325916/1‑96 (MQ=2)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                                           <  2:1884101/101‑1 (MQ=255)
                                            cTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                                            <  1:2607577/101‑7 (MQ=2)
                                            cTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                                            <  1:888322/101‑7 (MQ=2)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                                         >  2:425219/1‑101 (MQ=255)
                                              ccccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                                          >  1:566797/1‑93 (MQ=2)
                                              ccccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                                        <  2:138767/101‑1 (MQ=255)
                                               cccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatg                                                         >  2:1341136/1‑92 (MQ=2)
                                                ccccGCGGGGGGGGGGATCGAAACCGTGTGGAT...GGTCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                                                      <  1:1912947/101‑1 (MQ=255)
                                                 cccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                                                     <  2:1746796/101‑1 (MQ=255)
                                                   cgcgGGGGCGGGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGa                                                   <  2:3997498/101‑1 (MQ=255)
                                                    gcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAc                                                  <  2:2153071/101‑1 (MQ=255)
                                                      gggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                                <  2:2937060/101‑1 (MQ=255)
                                                       ggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                                               >  2:1817277/1‑101 (MQ=255)
                                                         ggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgc                                             <  2:2002214/101‑1 (MQ=255)
                                                           cGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgg                                           >  1:4021350/1‑101 (MQ=255)
                                                            gTGGATCGAAACCGTGTGGAT...GAACATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                                          <  1:2963823/101‑1 (MQ=255)
                                                             tGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgggc                                         >  2:3883074/1‑99 (MQ=255)
                                                             tGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                                         <  1:755158/101‑1 (MQ=255)
                                                              ggATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcc                                        >  1:281535/1‑101 (MQ=255)
                                                               gATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                                       <  2:3593606/101‑1 (MQ=255)
                                                               gATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                                       >  1:3593859/1‑101 (MQ=255)
                                                               gATCGAAACCGTGTGAAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                                       >  1:709995/1‑101 (MQ=255)
                                                                  cGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                                    <  2:577833/101‑1 (MQ=255)
                                                                    aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                                  <  1:1817397/101‑1 (MQ=255)
                                                                    aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                                  >  2:3431729/1‑101 (MQ=255)
                                                                    aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                                  >  2:4140656/1‑101 (MQ=255)
                                                                     aaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg                                 <  1:3704140/101‑1 (MQ=255)
                                                                     aaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg                                 >  1:2638134/1‑101 (MQ=255)
                                                                     aaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg                                 <  2:408046/101‑1 (MQ=255)
                                                                     aaCCGTGTGGAT...GATCATGCAGCGCGGACGGCGT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg                                 <  2:2097041/101‑1 (MQ=255)
                                                                        cGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg                              <  1:915776/101‑1 (MQ=255)
                                                                         ctgtgGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGt                             <  2:3985650/100‑1 (MQ=255)
                                                                           gtgGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg                           >  2:2400244/1‑101 (MQ=255)
                                                                              gat...gatCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTc                        >  1:3251616/1‑101 (MQ=255)
                                                                                t...gatCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc                      <  1:392631/101‑1 (MQ=255)
                                                                                t...gatCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc                      <  2:2772552/101‑1 (MQ=255)
                                                                                t...gatCATGCAGCGCGGACGGCTT.GGGTCGCT.CCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccc                     <  2:852797/101‑1 (MQ=255)
                                                                                     atCATGCAGCGCGGACGGCTT.GGGACGCTCCCCCGGCGGGGGCGTGGATCAAAAACGGTCCAGAGATAGACCGCGCGGACCTCCGCAGACGTCGCTccccc                    >  2:2824867/1‑101 (MQ=255)
                                                                                      tCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccccc                   >  1:3892199/1‑101 (MQ=255)
                                                                                            aGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCgggg             <  1:639434/101‑1 (MQ=255)
                                                                                            aGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCgggg             >  2:3097414/1‑101 (MQ=255)
                                                                                             gcgcGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCggggg            >  2:3896463/1‑101 (MQ=255)
                                                                                                cgGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGt         <  2:2372125/101‑1 (MQ=255)
                                                                                                  gACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTgg       <  1:3730902/101‑1 (MQ=255)
                                                                                                  gACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTgg       >  2:3482932/1‑101 (MQ=255)
                                                                                                    cGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGAt     >  1:3319695/1‑101 (MQ=255)
                                                                                                     ggCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATc    <  2:3132633/101‑1 (MQ=255)
                                                                                                       cTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa  <  1:2791716/101‑1 (MQ=255)
                                                                                                       cTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa  <  2:1138077/101‑1 (MQ=255)
                                                                                                       |                                                                                                     
TCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1396731‑1396931

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG