Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,944 Δ1 bp 14.7% coding (826/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9440G→.14.7% 130.9 ‑0.1 ‑0.4279coding (826/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/22):  ref base (114/123):  total (134/145)
Fisher's exact test strand distribution p-value = 8.67e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.55e-01

Only 100 of 304 total aligned reads displayed.

CCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCC  >  NC_014034/1396844‑1397044
                                                                                                         |                                                                                                            
ccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.cg.cg                                                                                                              <  1:3645866/101‑1 (MQ=255)
 cccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.Cg.g                                                                                                            <  1:1677428/101‑1 (MQ=255)
   cgcgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.Gc.c                                                                                                         <  1:4140918/101‑1 (MQ=255)
       ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.t                                                                                                    <  1:1993735/101‑1 (MQ=255)
        gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.Tc                                                                                                   <  2:1657081/101‑1 (MQ=255)
           cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGc                                                                                                >  1:1340677/1‑101 (MQ=255)
            gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCt                                                                                               >  1:4043265/1‑101 (MQ=255)
                aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.Tccc                                                                                          >  1:1108784/1‑100 (MQ=255)
                 tCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GG.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGc                                                                                         >  2:3372974/1‑101 (MQ=255)
                  cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGc.c                                                                                       <  2:2861881/101‑1 (MQ=255)
                   gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                      <  2:1847080/101‑1 (MQ=255)
                   gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                      <  1:1369072/101‑1 (MQ=255)
                   gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                      <  2:746177/101‑1 (MQ=255)
                     aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                  <  2:2637928/101‑1 (MQ=255)
                     aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                  <  2:2734588/101‑1 (MQ=255)
                         ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CCG..CGTg.g                                                                             >  1:2891896/1‑101 (MQ=255)
                           gccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTc                                                                           <  1:981186/100‑1 (MQ=255)
                             cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGc                                                                         >  2:417198/1‑101 (MQ=255)
                                 gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccc                                                                     <  1:2725962/101‑1 (MQ=255)
                                  aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GGCGCTcccc                                                                    >  2:3944976/1‑101 (MQ=255)
                                   tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                   >  1:1797242/1‑101 (MQ=255)
                                   tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                   >  1:2162195/1‑101 (MQ=255)
                                    aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                  <  1:2825086/101‑1 (MQ=255)
                                    aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                  <  2:4043004/101‑1 (MQ=255)
                                     gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCcg                                                                 <  2:1761561/101‑1 (MQ=255)
                                        cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCgg                                                              <  1:1909163/101‑1 (MQ=255)
                                         gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                             >  2:2183098/1‑101 (MQ=255)
                                             ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                         >  2:3419439/1‑101 (MQ=255)
                                             ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                         >  1:4180781/1‑101 (MQ=255)
                                             ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                         <  1:1080464/101‑1 (MQ=255)
                                             ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                         >  2:950168/1‑101 (MQ=255)
                                               aCCTCCTCCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                                       <  2:432769/101‑1 (MQ=255)
                                                 tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                      <  1:58498/92‑1 (MQ=0)
                                                 ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                     <  2:281490/101‑1 (MQ=255)
                                                 ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                     <  1:2127599/101‑1 (MQ=255)
                                                  ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  1:777828/92‑1 (MQ=2)
                                                  ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                    >  1:4226500/10‑101 (MQ=2)
                                                  tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                    >  1:1018959/1‑101 (MQ=255)
                                                   tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  1:875586/93‑1 (MQ=2)
                                                   gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  2:1243074/94‑1 (MQ=2)
                                                   acccgcccGTCGCTCCCCCCGCGGGGGCGTAGATCGAAACT.GC.GT.G.CG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                      >  1:3769715/8‑101 (MQ=0)
                                                    ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  <  2:3232107/94‑1 (MQ=2)
                                                    cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  2:3268080/95‑1 (MQ=2)
                                                    caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  2:2465886/94‑1 (MQ=9)
                                                     ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  <  1:521938/96‑1 (MQ=2)
                                                     ccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.CG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  2:1557810/96‑1 (MQ=2)
                                                      cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                >  1:3035506/1‑101 (MQ=255)
                                                      cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                >  2:1314058/1‑101 (MQ=255)
                                                      acactggcGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCCC.GC.CGCG.CCAGATCTTTGT.CTG..GGCG.GGGGCTCCCCCCGCGGGGGCGTGCATCGa                                                 <  2:1965481/94‑1 (MQ=2)
                                                      acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                 >  2:3598272/6‑101 (MQ=9)
                                                       gaccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                               >  2:1579462/4‑101 (MQ=255)
                                                         gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                              <  1:3900838/100‑1 (MQ=2)
                                                         gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                              <  2:2561397/100‑1 (MQ=2)
                                                         cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                             <  2:594928/99‑2 (MQ=2)
                                                          tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                             <  2:2383205/100‑2 (MQ=9)
                                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                           <  2:3711751/101‑1 (MQ=255)
                                                           gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                           <  1:3594269/101‑1 (MQ=255)
                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAACATCa                                          >  2:1906603/1‑101 (MQ=255)
                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                          <  1:2235987/101‑5 (MQ=2)
                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                          <  1:7030/101‑5 (MQ=2)
                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                           <  1:3387187/101‑4 (MQ=9)
                                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.CG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                            <  2:96623/101‑2 (MQ=2)
                                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.CG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCACCCCGCGGGGGCGCGGATCGAAACac                                            >  2:3513049/1‑100 (MQ=2)
                                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                          >  2:3472945/1‑97 (MQ=2)
                                                             cGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAg                                         <  2:3315692/101‑1 (MQ=255)
                                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                         >  2:3732868/1‑96 (MQ=2)
                                                               cTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.GG.CG.GCATGGATTCATCA.ACCGC.CAT..CGAGCGTCCCTCCCCCCGCGGGGGCGTGGGACGaaaaacca                                          >  2:2610838/1‑96 (MQ=2)
                                                               cTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                        <  2:3087523/101‑7 (MQ=2)
                                                               cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCAAT.GCCG.AA.GC.CAAG.GCCGCA.GCAGC.GGC..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAACGAAACaaaaa                                         >  1:1778079/1‑97 (MQ=2)
                                                                tCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                      >  1:1948322/1‑101 (MQ=255)
                                                                tCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                      >  1:1045238/1‑101 (MQ=255)
                                                                tCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                      <  2:658004/101‑9 (MQ=2)
                                                                tCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                       >  1:3920103/1‑94 (MQ=9)
                                                                cccccccGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                       <  1:1246585/100‑8 (MQ=9)
                                                                 ccccccGCGGGGGCGTGGATCGAAACT.GC.GT.G.CG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                        >  1:1720171/1‑99 (MQ=2)
                                                                 ccccccGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacg                                      >  1:658081/1‑93 (MQ=0)
                                                                  cccccGCGGGGGCGTGGATCGAAACT.GC.GT.G.CG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatg                                       <  2:1183173/101‑4 (MQ=2)
                                                                  cccccGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                     <  2:798709/101‑10 (MQ=9)
                                                                  cccccGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                     <  2:1390535/101‑10 (MQ=9)
                                                                   ccccGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAActcggcctcga                                    >  2:471760/1‑91 (MQ=2)
                                                                    cccGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGAGCtcgtcg                                  >  1:4113510/1‑101 (MQ=255)
                                                                    cccGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                  <  1:1522105/101‑1 (MQ=255)
                                                                    cccGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                  >  1:3518724/1‑101 (MQ=255)
                                                                      cgcgGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACATCAGCTCGTCGtt                                <  1:2400420/101‑1 (MQ=255)
                                                                      cgcgGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                <  2:2427774/101‑1 (MQ=255)
                                                                       gcgGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGTCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                               <  2:206652/101‑1 (MQ=255)
                                                                        cgGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                              >  1:3257945/1‑101 (MQ=255)
                                                                        cgGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                              >  1:2586634/1‑101 (MQ=255)
                                                                           gggCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                           <  2:3165396/101‑1 (MQ=255)
                                                                            ggCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAg                          <  1:1579571/101‑1 (MQ=255)
                                                                             gCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                         <  2:2961625/101‑1 (MQ=255)
                                                                             gCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                         <  2:1797123/101‑1 (MQ=255)
                                                                                   aTCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                   <  2:3891940/101‑1 (MQ=255)
                                                                                   aTCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                   <  2:1077494/101‑1 (MQ=255)
                                                                                      gAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCTCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTCGTCAGCCGGACGATg                >  1:476185/1‑101 (MQ=255)
                                                                                          cA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg            <  1:3945236/101‑1 (MQ=255)
                                                                                                cT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGGTGTCCGTCGc        >  1:3720226/1‑101 (MQ=255)
                                                                                                 t.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt       <  1:3125621/101‑1 (MQ=255)
                                                                                                 t.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt       <  2:1108690/101‑1 (MQ=255)
                                                                                                   g.cg.cG.GC.CGCA.GCGGCG.TCCGC.CTG..CGTG.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc      <  1:3296079/101‑1 (MQ=255)
                                                                                                         |                                                                                                            
CCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCC  >  NC_014034/1396844‑1397044

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG