Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,945 Δ1 bp 14.7% coding (827/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9450G→.14.7% 130.9 ‑0.1 ‑0.5281coding (827/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/22):  ref base (108/115):  total (134/147)
Fisher's exact test strand distribution p-value = 8.66e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.03e-01

Only 100 of 304 total aligned reads displayed.

CCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCC  >  NC_014034/1396845‑1397045
                                                                                                        |                                                                                                           
  cgcgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.Gc.c                                                                                                          <  1:4140918/101‑1 (MQ=255)
      ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.t                                                                                                     <  1:1864599/101‑1 (MQ=255)
       gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.Tc                                                                                                    <  2:1657081/101‑1 (MQ=255)
          cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGc                                                                                                 >  1:1340677/1‑101 (MQ=255)
          cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGc                                                                                                 <  1:1495139/101‑1 (MQ=255)
           gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCt                                                                                                >  1:4043265/1‑101 (MQ=255)
           gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCt                                                                                                >  1:3098189/1‑101 (MQ=255)
               aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.Tccc                                                                                           >  1:1108784/1‑100 (MQ=255)
                tCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GGCT.GCG.CG.GC.CGCA.TCGGCT.TCCGc                                                                                          >  2:3372974/1‑101 (MQ=255)
                 cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGc.c                                                                                        <  2:2861881/101‑1 (MQ=255)
                  gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                       <  1:3280969/101‑1 (MQ=255)
                  gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                       <  2:1847080/101‑1 (MQ=255)
                  gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                       <  1:1369072/101‑1 (MQ=255)
                  gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                       <  2:746177/101‑1 (MQ=255)
                   aaaCCGGTCCAGAGATAGACCGCGCGTACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTg                                                                                      <  1:2547354/101‑1 (MQ=255)
                    aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                   <  2:2637928/101‑1 (MQ=255)
                    aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                   <  2:2734588/101‑1 (MQ=255)
                          gccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTc                                                                            <  1:981186/100‑1 (MQ=255)
                            cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGc                                                                          >  2:417198/1‑101 (MQ=255)
                                 aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GGCGCTcccc                                                                     >  2:3944976/1‑101 (MQ=255)
                                   aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                   <  2:4043004/101‑1 (MQ=255)
                                    gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCcg                                                                  <  2:1761561/101‑1 (MQ=255)
                                        gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                              >  2:2183098/1‑101 (MQ=255)
                                            ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                          <  1:1080464/101‑1 (MQ=255)
                                            ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                          >  2:950168/1‑101 (MQ=255)
                                            ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                          >  2:3419439/1‑101 (MQ=255)
                                            ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                          >  1:4180781/1‑101 (MQ=255)
                                              aCCTCCTCCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                                        <  2:432769/101‑1 (MQ=255)
                                               cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                       >  1:2823536/1‑101 (MQ=255)
                                                tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                       <  1:58498/92‑1 (MQ=0)
                                                ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                      <  2:281490/101‑1 (MQ=255)
                                                attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                      <  1:290828/91‑1 (MQ=0)
                                                attggccccggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                      <  1:1786308/91‑1 (MQ=0)
                                                 ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                     >  1:4226500/10‑101 (MQ=2)
                                                 ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                     <  1:777828/92‑1 (MQ=2)
                                                 tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                     >  1:1018959/1‑101 (MQ=255)
                                                  tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                    <  1:875586/93‑1 (MQ=2)
                                                  gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                     <  2:1243074/94‑1 (MQ=2)
                                                  ccccgcccGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                       <  1:268214/94‑1 (MQ=0)
                                                  acccgcccGTCGCTCCCCCCGCGGGGGCGTAGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                       >  1:3769715/8‑101 (MQ=0)
                                                   ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   <  2:3232107/94‑1 (MQ=2)
                                                   cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                    <  2:3268080/95‑1 (MQ=2)
                                                   caactctgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                    <  1:2149614/94‑1 (MQ=9)
                                                   caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                    <  2:2465886/94‑1 (MQ=9)
                                                    ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   <  1:521938/96‑1 (MQ=2)
                                                    ccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                     <  2:1557810/96‑1 (MQ=2)
                                                     gttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                  <  1:2234409/97‑1 (MQ=2)
                                                     ccccggTCGCTCACCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                 >  1:2289869/6‑101 (MQ=2)
                                                     cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                 >  2:1314058/1‑101 (MQ=255)
                                                     acactggcGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCCC.GC.CGCG.CCAGATCTTTGT.CTG..GGCG.GGGGCTCCCCCCGCGGGGGCGTGCATCGa                                                  <  2:1965481/94‑1 (MQ=2)
                                                     acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                  >  2:3598272/6‑101 (MQ=9)
                                                      gaccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                >  2:1579462/4‑101 (MQ=255)
                                                        gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                               <  1:3900838/100‑1 (MQ=2)
                                                        gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                               <  2:2561397/100‑1 (MQ=2)
                                                        cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                              <  2:594928/99‑2 (MQ=2)
                                                         tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                              <  2:2383205/100‑2 (MQ=9)
                                                         tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                              >  1:1676223/2‑100 (MQ=9)
                                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                            <  2:3711751/101‑1 (MQ=255)
                                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                           <  1:363515/101‑1 (MQ=255)
                                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAACATCa                                           >  2:1906603/1‑101 (MQ=255)
                                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                           <  1:7030/101‑5 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                             <  2:96623/101‑2 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCACCCCGCGGGGGCGCGGATCGAAACac                                             >  2:3513049/1‑100 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                           >  2:3472945/1‑97 (MQ=2)
                                                            cGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAg                                          <  2:3315692/101‑1 (MQ=255)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                          >  2:3732868/1‑96 (MQ=2)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCAGCGGGGGCGTGGATCGAAActacacc                                         >  1:3552116/1‑95 (MQ=2)
                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GGG.CG.GCATGGATTCATCA.ACCGC.CAT..CGAGCGTCCCTCCCCCCGCGGGGGCGTGGGACGaaaaacca                                           >  2:2610838/1‑96 (MQ=2)
                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                         <  2:3087523/101‑7 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                       >  1:287457/1‑101 (MQ=255)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                       >  1:1045238/1‑101 (MQ=255)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                       <  2:658004/101‑9 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                       <  1:2095289/101‑9 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                        >  1:3920103/1‑94 (MQ=9)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                        >  1:3196182/1‑94 (MQ=9)
                                                               cccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                        <  1:1246585/100‑8 (MQ=9)
                                                                ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                         <  1:1693580/101‑3 (MQ=2)
                                                                ccccccGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacg                                       >  1:658081/1‑93 (MQ=0)
                                                                ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                       <  1:1972157/101‑9 (MQ=9)
                                                                ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                       <  1:1551679/101‑9 (MQ=9)
                                                                 cccccGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatg                                        <  2:1183173/101‑4 (MQ=2)
                                                                 cccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                      <  2:798709/101‑10 (MQ=9)
                                                                 cccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                      <  2:1390535/101‑10 (MQ=9)
                                                                  ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAActcggcctcga                                     >  2:471760/1‑91 (MQ=2)
                                                                   cccGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGAGCtcgtcg                                   >  1:4113510/1‑101 (MQ=255)
                                                                   cccGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                   <  1:351593/101‑1 (MQ=255)
                                                                     cgcgGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                    <  1:1934933/101‑8 (MQ=0)
                                                                     cgcgGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                 <  2:2427774/101‑1 (MQ=255)
                                                                      gcgGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGTCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                                <  2:206652/101‑1 (MQ=255)
                                                                          gggCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                            <  2:3165396/101‑1 (MQ=255)
                                                                            gCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                          <  2:2961625/101‑1 (MQ=255)
                                                                            gCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                          <  2:1797123/101‑1 (MQ=255)
                                                                                 gagcGAAACA.TCCT.GCG.CG.GC.CGCA.TCTGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                     <  1:3373219/98‑1 (MQ=255)
                                                                                  aTCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                    <  2:3891940/101‑1 (MQ=255)
                                                                                  aTCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                    <  2:1077494/101‑1 (MQ=255)
                                                                                     gAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCTCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTCGTCAGCCGGACGATg                 >  1:476185/1‑101 (MQ=255)
                                                                                         cA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg             <  1:3945236/101‑1 (MQ=255)
                                                                                            gCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc           >  1:1747365/1‑101 (MQ=255)
                                                                                              cT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGGTGTCCGTCGc         >  1:3720226/1‑101 (MQ=255)
                                                                                               t.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt        <  2:1108690/101‑1 (MQ=255)
                                                                                                        |                                                                                                           
CCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCC  >  NC_014034/1396845‑1397045

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG