Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,607,985 Δ1 bp 100% intergenic (+255/‑92) RCAP_rcc01484 → / → murD hypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,607,9850T→.100.0% 310.1 73intergenic (+255/‑92)RCAP_rcc01484/murDhypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase
Reads supporting (aligned to +/- strand):  new base (25/48):  ref base (0/0):  total (25/48)

CCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGGCCGCGCGGCGCGGGCCCAATTCACCCCCGCAGCTTACGGA  >  NC_014034/1607881‑1608068
                                                                                                        |                                                                                    
cccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCt                                                                                      <  1:1288737/101‑1 (MQ=255)
 ccTCAAGGATGCCGGCGGCCC.GGGAATGCGGGC.AGACCGGG.CGGGCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtg                                                                                     <  1:3231825/101‑2 (MQ=255)
  cTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGCCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgc                                                                                    <  1:2426758/101‑3 (MQ=255)
  cTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgc                                                                                    <  2:1767726/101‑3 (MQ=255)
  cTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgc                                                                                    >  2:988675/1‑99 (MQ=255)
   tCAAGGATGGCGGCGGCCC.GGGAATGCGGGCG.GACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcg                                                                                   <  1:157541/101‑4 (MQ=255)
    cAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcgg                                                                                  >  2:3847527/1‑97 (MQ=255)
       ggAGGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGgcgc                                                                              <  1:80805/101‑1 (MQ=255)
        gATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgcc                                                                             <  1:3984084/101‑1 (MQ=255)
           ggcggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCgccgcc                                                                          >  1:1267746/1‑101 (MQ=255)
            gcggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGccc                                                                         <  1:2224571/101‑1 (MQ=255)
             gggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg                                                                        <  2:1963808/100‑1 (MQ=255)
             gggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg                                                                        <  1:333599/100‑1 (MQ=255)
             cggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg                                                                        <  2:3420050/101‑1 (MQ=255)
              ggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.a                                                                      <  1:655941/101‑1 (MQ=255)
              ggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.a                                                                      >  1:3775511/1‑101 (MQ=255)
               gcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.aa                                                                     >  2:3707763/1‑101 (MQ=255)
                 ggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.ACAg                                                                   >  2:3003848/1‑101 (MQ=255)
                    cccgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAggg                                                                 <  1:2959051/98‑1 (MQ=255)
                     ccgggCATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCCACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAgggg                                                                >  2:723611/3‑101 (MQ=255)
                     ccgggAAGGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAgggg                                                                <  2:3936072/99‑1 (MQ=255)
                      cgggAATGCGGGC.AGCCCGGGCC.CACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAggggg                                                               <  1:418410/100‑1 (MQ=255)
                      cgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAGagggg                                                               >  2:3977831/2‑101 (MQ=255)
                      cgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAggggg                                                               <  2:489757/100‑1 (MQ=255)
                      cgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAggggg                                                               >  1:549348/2‑101 (MQ=255)
                      cgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAgcggg                                                               >  2:1307088/2‑101 (MQ=255)
                       gggAATGCGGGC.AGCCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAgggggg                                                              <  2:752124/101‑1 (MQ=255)
                       gggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGACTGCGACGCC.TGCGGCGCCGCCCG.AAAgggggg                                                              >  1:3646819/1‑101 (MQ=255)
                       ggaattGCGGGC.AGCCCGGG.CGGACCGCCGCCTGTC.TCCGGCCTTTTCCCAGCGGCTCCCGCGACGGGGTGCGCCGCC.TGCGGCGCCGCCCG.AAAgggggg                                                              <  2:3775254/96‑1 (MQ=255)
                           aTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTgc                                                          >  2:2653209/1‑101 (MQ=255)
                           aTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTgc                                                          >  2:572512/1‑101 (MQ=255)
                           aTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTgc                                                          <  1:1004141/101‑1 (MQ=255)
                               cgggcagaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTgcgcg                                                       <  2:1976636/96‑1 (MQ=255)
                               cgggcagaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCGgcgcg                                                       >  2:253758/6‑101 (MQ=255)
                                   cagcccGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGcccc                                                   <  2:3216877/97‑1 (MQ=255)
                                   cagaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGcccc                                                   <  1:2134308/100‑1 (MQ=255)
                                    agaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGccccc                                                  <  2:3873997/101‑1 (MQ=255)
                                      aCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGccccccc                                                >  2:2354490/1‑100 (MQ=255)
                                       ccGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCctc                                               <  2:679940/101‑1 (MQ=255)
                                       ccGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCctc                                               >  1:4016919/1‑101 (MQ=255)
                                        cGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCctct                                              <  2:3786267/101‑1 (MQ=255)
                                         gggCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCtt                                             >  2:1149250/1‑101 (MQ=255)
                                         gggCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCCCtt                                             >  1:2334439/1‑101 (MQ=255)
                                           ggccgacccccgcCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTg                                            <  2:2233483/91‑1 (MQ=255)
                                           ggccgACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGCGCGACGCC.TGCGGCGCCGCCCG.CAGCAGCGGCAGCGCGccccccccccc                                            >  2:1467115/5‑96 (MQ=255)
                                               gcccGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTcc                                        <  2:3995708/99‑1 (MQ=38)
                                               gcccGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTcc                                        <  1:1655572/99‑1 (MQ=38)
                                               gACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTcc                                        >  2:2903541/1‑101 (MQ=38)
                                                aCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCGGCGCCACCCCCCCTTggttcg                                       >  1:1866039/1‑97 (MQ=255)
                                                   gccgcCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCcgctg                                    <  2:3354675/99‑3 (MQ=38)
                                                   gccgcCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCcgctg                                    <  2:417877/99‑3 (MQ=38)
                                                     cgcCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCcgctggc                                  <  2:3878939/101‑5 (MQ=38)
                                                     cgcCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCcgctggc                                  <  1:2506351/101‑5 (MQ=38)
                                                           gtctCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAAGGGGGGCTGCGCGCCCCCCCCTT.GCCCCcccggcgcgg                             >  1:837581/4‑101 (MQ=255)
                                                            tctCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCgc.tggcgcggcc                           <  1:779051/99‑3 (MQ=38)
                                                             ctCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGCGGCcc                          >  1:368559/2‑101 (MQ=38)
                                                             ctCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGCGGCcc                          <  2:2466092/100‑1 (MQ=38)
                                                             ctCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGCGGCcc                          >  1:4203524/2‑101 (MQ=38)
                                                              tCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGCGGCcca                         <  2:558129/101‑2 (MQ=38)
                                                                cGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAAt                      <  1:2243143/101‑1 (MQ=37)
                                                                cGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCCTGGTCCGC.TGGCGC.GGCCCAAt                      >  2:1782260/1‑101 (MQ=255)
                                                                 gACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAAtt                     <  2:11731/101‑1 (MQ=37)
                                                                 gACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAAtt                     <  1:1061500/101‑1 (MQ=37)
                                                                   ccTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCa                   <  1:828935/101‑1 (MQ=37)
                                                                      tttCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCAccc                <  2:2724014/101‑1 (MQ=38)
                                                                      tttCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCAccc                <  1:3708017/101‑1 (MQ=37)
                                                                       ttCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCAcccc               >  2:3088370/1‑101 (MQ=38)
                                                                       ttCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCAcccc               <  1:677789/101‑1 (MQ=37)
                                                                       ttCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCAcccc               <  1:959689/101‑1 (MQ=38)
                                                                        tCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCAccccc              <  1:3004073/101‑1 (MQ=38)
                                                                         cccAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCACCCCCg             <  2:159319/101‑1 (MQ=38)
                                                                          ccAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCACCCCCGc            <  1:1880069/101‑1 (MQ=38)
                                                                           cAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCACCCCCGcg           >  1:1497020/1‑100 (MQ=38)
                                                                            aGCGGATCCCGCGACGGGGTGCGACGCC.TGCAGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCACCCCCGcgg          <  1:4066694/101‑3 (MQ=37)
                                                                              cGGATCCCGCGACGGGGTGCGGCGCC.TGCGGCCCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCACCCCCGcggga        <  1:2791024/101‑5 (MQ=25)
                                                                              cGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCACCCCCGcggga        >  2:1869800/1‑97 (MQ=37)
                                                                              cGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATCCACCCCCGcggga        >  2:1133022/1‑97 (MQ=37)
                                                                               agATCTCGAGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGC.CCGGCCCAATCCACCCCCGcgggat       <  2:3422457/100‑6 (MQ=19)
                                                                                 gtCCCGCGGCGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCACCCCCGcgggatat     <  1:4066160/100‑8 (MQ=25)
                                                                                 aTCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCACCCCCGcgggatat     <  1:10768/101‑8 (MQ=37)
                                                                                  tCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCACCCCCGcgggatatt    <  1:1048526/101‑9 (MQ=37)
                                                                                  tCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCACCCCCGcgggatatt    <  1:4175435/101‑9 (MQ=37)
                                                                                    ccGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCACCCCCGcgggatatttc  <  2:439932/101‑11 (MQ=25)
                                                                                    ccGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCACCCCCGcgggatatttc  <  1:2662624/101‑11 (MQ=25)
                                                                                    ccGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGTCCGC.TGGCGC.GGCCCAATTCACCCCCGcgggatatttc  <  2:3109789/101‑11 (MQ=25)
                                                                                                        |                                                                                    
CCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCG.AAAGGGGGGCTGCGCGCCCCCCTCTTGGGCCGCGCGGCGCGGGCCCAATTCACCCCCGCAGCTTACGGA  >  NC_014034/1607881‑1608068

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG