BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,696,206	Δ1 bp	100%	intergenic (‑134/+91)	RCAP_rcc01564 ← / ← mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,696,206	0	G→.	100.0%	569.0	163	intergenic (‑134/+91)	RCAP_rcc01564/mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand): new base (65/97): ref base (0/1): total (65/98)

Only 100 of 171 total aligned reads displayed.


GTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCT.GATCGTGCCTCAGTCGAGC  >  NC_014034/1696106‑1696306


gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAAGgg                                                                                                      >  1:3860893/1‑101 (MQ=255)


  ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.CGCCCCTCCTGGCCTTTCGGCCAATTAAC.CCCGGGATTTTTGGGCCAAGggga                                                                                                    >  1:356887/1‑100 (MQ=255)


      tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                                              >  1:2382112/1‑100 (MQ=255)


       ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacac                                                                                             >  2:1742628/1‑99 (MQ=12)


         cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                                           >  2:2170299/1‑97 (MQ=255)


          gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                          <  1:926410/101‑6 (MQ=255)


          gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                          <  2:3443688/101‑6 (MQ=255)


          gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                          <  2:778379/101‑6 (MQ=255)


          gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                          <  2:939157/101‑6 (MQ=255)


           aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                         <  1:457876/101‑7 (MQ=12)


           aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                         <  1:1311932/101‑7 (MQ=12)


           aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                         <  2:3495203/101‑7 (MQ=12)


           aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                         <  1:1201293/101‑7 (MQ=12)


           aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                         <  2:1964853/101‑7 (MQ=12)


            aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                                        >  2:3054120/1‑94 (MQ=255)


            aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                                        >  1:2229965/1‑94 (MQ=255)


              ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                                      <  1:3498769/101‑10 (MQ=9)


              ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                                      <  1:863606/101‑10 (MQ=9)


               cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                                                     >  1:2091301/1‑91 (MQ=255)


                  gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                                >  2:3743203/1‑101 (MQ=255)


                  gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                                >  2:3075019/1‑101 (MQ=255)


                  gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                                <  1:4189620/101‑1 (MQ=255)


                   cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCCC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                                               <  1:2616245/101‑1 (MQ=255)


                   cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                                               <  2:2532239/101‑1 (MQ=255)


                   cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                                               >  2:3313167/1‑101 (MQ=9)


                    aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                              <  2:1108890/101‑1 (MQ=9)


                    aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                              <  2:53398/101‑1 (MQ=9)


                       aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCACTTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTggg                                                                           >  1:2325921/1‑100 (MQ=255)


                       aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                                           >  2:2386895/1‑101 (MQ=255)


                         ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                                         <  1:1570650/101‑1 (MQ=255)


                           ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                                       >  1:2793385/1‑101 (MQ=255)


                            tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                                      <  2:4000554/101‑1 (MQ=255)


                            tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                                      >  2:51160/1‑101 (MQ=9)


                             gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                                     <  1:3811798/101‑1 (MQ=9)


                             gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                                     <  2:3736705/101‑1 (MQ=9)


                              cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                                    <  1:4119107/101‑1 (MQ=255)


                              cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                                    <  1:1735552/101‑1 (MQ=255)


                                aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCCA..GGCGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATTCg                                                                  >  2:3860695/1‑101 (MQ=255)


                                 ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                                 >  2:165009/1‑101 (MQ=255)


                                 ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                                 >  2:3208885/1‑101 (MQ=255)


                                  gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                                >  1:2264501/1‑101 (MQ=9)


                                  gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                                <  1:413763/101‑1 (MQ=255)


                                   aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                               <  2:17495/101‑1 (MQ=9)


                                   aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                               >  2:4073574/1‑101 (MQ=12)


                                     gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                             <  2:2359735/101‑1 (MQ=255)


                                     gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                             >  1:150170/1‑101 (MQ=9)


                                     gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                             <  1:214203/101‑1 (MQ=255)


                                     gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                             <  1:3730797/101‑1 (MQ=255)


                                           gaggggcgctGCCTCTCCTGGCCTTTCGGCCAAGTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                                         <  1:3640761/92‑1 (MQ=9)


                                           gaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTTGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTTGGGCAAATACGCCTCTGGcc                                                         >  1:3758137/10‑101 (MQ=255)


                                            aggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCTC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTTCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                                        <  2:1706647/93‑1 (MQ=255)


                                             ggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                                                       <  2:3243033/94‑1 (MQ=255)


                                               ggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGaa                                                     >  1:1084837/6‑101 (MQ=16)


                                                gcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                                                    <  1:1531263/97‑1 (MQ=255)


                                                gcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                                                    <  2:536233/97‑1 (MQ=16)


                                                gcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                                                    <  1:1361192/97‑1 (MQ=255)


                                                 cgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                                   <  1:3046737/98‑1 (MQ=16)


                                                  gctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg                                                  >  2:3703779/3‑101 (MQ=16)


                                                  gctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg                                                  <  1:2434553/99‑1 (MQ=255)


                                                   ctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                                                 >  2:2870644/2‑101 (MQ=16)


                                                   ctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                                                 <  2:2091163/100‑1 (MQ=255)


                                                    tGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                                <  1:785993/101‑1 (MQ=16)


                                                    tGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                                >  2:1248457/1‑101 (MQ=255)


                                                    tGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                                <  2:3226963/101‑1 (MQ=16)


                                                     gCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                               >  2:162784/1‑101 (MQ=19)


                                                     gCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                               <  2:3342690/101‑1 (MQ=255)


                                                     gCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                               >  2:2191513/1‑101 (MQ=19)


                                                     gCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                               <  1:641471/101‑1 (MQ=255)


                                                      ccccTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                              >  1:1000892/1‑101 (MQ=19)


                                                      ccccTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                              >  1:3321665/1‑101 (MQ=16)


                                                      ccccTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                              <  2:1412046/101‑1 (MQ=19)


                                                         cTCCTGGCCTTTCGGCCAATTCCC.CCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcg                                           <  1:2978002/101‑1 (MQ=21)


                                                              ggCCTTTCGG.CCAATCCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                                      <  2:2414690/101‑1 (MQ=19)


                                                                 cTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                                   <  1:3209124/101‑1 (MQ=19)


                                                                 cTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCC.AAGGG.GAAAC..CCCTTGCTTTTTCTTGGCGCAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                                   <  1:2564489/101‑1 (MQ=255)


                                                                    tcggccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCCTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcg                                <  2:3660492/96‑1 (MQ=255)


                                                                     cggccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc                               >  2:2594750/5‑101 (MQ=255)


                                                                      ggccAATTCCC.CCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg                              <  1:3704033/98‑1 (MQ=25)


                                                                      ggccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg                              >  1:1035999/4‑101 (MQ=255)


                                                                       gccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCgg                             <  2:3840733/99‑1 (MQ=25)


                                                                        ccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGc                            >  1:2693596/2‑101 (MQ=25)


                                                                         cAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCTGCGCCGAAGGCGCGGcc                           >  1:2508434/1‑101 (MQ=25)


                                                                         cAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc                           >  2:1878215/1‑101 (MQ=255)


                                                                          aaTTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc                          >  2:855119/1‑101 (MQ=255)


                                                                           aTTCCC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccc                         <  2:2025703/101‑1 (MQ=25)


                                                                               ccccccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCGAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt                      <  2:2446638/98‑1 (MQ=255)


                                                                               ccccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt                      <  2:2593751/98‑1 (MQ=25)


                                                                                accccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGTCGCGGCCCCCCT.g                    <  1:2824290/99‑1 (MQ=25)


                                                                                  cccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCT.GAt                  <  2:2229806/101‑1 (MQ=37)


                                                                                  cccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCT.GAt                  >  1:1826280/1‑101 (MQ=255)


                                                                                   ccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCT.GATc                 <  1:2900142/101‑1 (MQ=37)


                                                                                      ggatgtttGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCT.GATCGTg              <  1:1742747/95‑1 (MQ=37)


                                                                                      ggatgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCT.GATCGTg              >  1:462327/7‑101 (MQ=255)


                                                                                        atgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCT.GATCGTGcc            >  2:1366237/5‑101 (MQ=255)


                                                                                        atgtttGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTAGATCggaa             >  1:1500228/5‑98 (MQ=255)


                                                                                         tgtttGGGCCAA..GGTG.AACCA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCT.GATCGTGCCt           <  2:102796/98‑1 (MQ=255)


                                                                                           gTTGGGGCCAA.GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCT.GATCGTGCCTc          <  1:3313408/101‑1 (MQ=37)


                                                                                            ttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCT.GATCGTGCCTCAg        >  1:3174659/1‑101 (MQ=255)


                                                                                            ttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCT.GATCGTGCCTCAg        >  2:779532/1‑101 (MQ=255)


                                                                                                    caGGGTGAAAC..CCCTTGCTTTTTCTGGGCACAAATACGCCTCTGGCCCGAAGGGAAAGCCGCGCCGAAGGCGCGGCCCCCCT.GATCGTGCCTCAGTCGAgc  <  2:564166/100‑1 (MQ=255)


GTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCT.GATCGTGCCTCAGTCGAGC  >  NC_014034/1696106‑1696306

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG