Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,786,540 Δ1 bp 94.8% coding (674/819 nt) RCAP_rcc01648 → ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,786,5400C→.94.8% 27.0 0.0 ‑1.5210coding (674/819 nt)RCAP_rcc01648ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (94/105):  ref base (5/5):  total (99/111)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.10e-02

Only 100 of 214 total aligned reads displayed.

AGCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GG.CCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCT  >  NC_014034/1786441‑1786636
                                                                                                     |                                                                                                
aGCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGc                                                                                                  >  2:3541392/1‑101 (MQ=255)
aGCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGc                                                                                                  <  1:3298290/101‑1 (MQ=255)
  cTGATGCCGTATCTGAGCGGCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGctt                                                                                                <  1:3684225/101‑3 (MQ=255)
  cTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGctt                                                                                                <  2:3040830/101‑3 (MQ=255)
   tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGCTtt                                                                                               >  2:1858834/1‑101 (MQ=255)
   tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGCTtt                                                                                               >  2:3572386/1‑101 (MQ=255)
   tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGCTtt                                                                                               <  1:1795332/101‑1 (MQ=255)
   tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGGGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGCTtt                                                                                               <  2:3604613/101‑1 (MQ=255)
    gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGCTttc                                                                                              <  1:3541645/101‑2 (MQ=255)
     aTGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCg                                                                                            <  2:1568466/101‑1 (MQ=255)
     aTGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCg                                                                                            >  2:2029295/1‑101 (MQ=255)
       gCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTa                                                                                          >  2:531289/1‑101 (MQ=255)
        ccGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTAt                                                                                         <  2:700834/101‑1 (MQ=255)
          gCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATcc                                                                                       >  1:225122/1‑101 (MQ=255)
          gCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATcc                                                                                       <  1:1727883/101‑1 (MQ=255)
           cATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCa                                                                                      <  1:502652/101‑1 (MQ=255)
             tCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATc                                                                                    >  1:1360977/1‑101 (MQ=255)
             tCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATc                                                                                    >  2:781729/1‑101 (MQ=255)
              cTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCt                                                                                   >  2:4168913/1‑101 (MQ=255)
               tGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTg                                                                                  >  2:104488/1‑101 (MQ=255)
                 aTCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGaa                                                                                >  2:323229/1‑101 (MQ=255)
                     ccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTc                                                                            <  1:12547/101‑1 (MQ=255)
                     ccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTc                                                                            <  1:654251/101‑1 (MQ=255)
                      cgcggcgcggGCGGTGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCGCGAACGGGATCGG.CTTTCGTATCCTCCTGGATTTcc                                                                           <  2:2815401/101‑1 (MQ=255)
                      cgcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                           >  2:4145584/1‑101 (MQ=255)
                      cgcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGGGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                           >  1:1674511/1‑101 (MQ=255)
                        cggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAg                                                                         <  1:2690307/101‑1 (MQ=255)
                         ggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGa                                                                        <  2:2316059/101‑1 (MQ=255)
                         ggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGa                                                                        >  2:2363065/1‑101 (MQ=255)
                          gcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGAt                                                                       >  2:1219544/1‑101 (MQ=255)
                           cgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATg                                                                      >  1:2163050/1‑101 (MQ=255)
                              gggcggggCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                   >  1:743789/1‑101 (MQ=255)
                              gggcggggCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                   <  2:2644633/101‑1 (MQ=255)
                              gggcggggCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                   >  2:727308/1‑101 (MQ=255)
                                gcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGa                                                                 <  1:1927739/101‑1 (MQ=255)
                                 cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                <  1:3596595/95‑1 (MQ=255)
                                 cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                >  2:3496500/7‑101 (MQ=255)
                                 cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                <  1:1476507/95‑1 (MQ=255)
                                  ggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACa                                                               <  1:2729173/96‑1 (MQ=255)
                                  ggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACa                                                               <  2:1253658/96‑1 (MQ=255)
                                    ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                             <  1:3162029/98‑1 (MQ=255)
                                    ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                             <  2:2901463/98‑1 (MQ=255)
                                    ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                             <  2:1894870/98‑1 (MQ=255)
                                    ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                             <  2:3952922/98‑1 (MQ=255)
                                     ggTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTg                                                            <  2:838955/100‑1 (MQ=255)
                                       tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGcc                                                          <  1:2774502/101‑1 (MQ=255)
                                       tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGcc                                                          >  1:1551560/1‑101 (MQ=255)
                                        ttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCg                                                         <  2:2119511/101‑1 (MQ=255)
                                        ttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCg                                                         >  1:1017334/1‑101 (MQ=255)
                                            cTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGt                                                     <  1:209521/101‑1 (MQ=255)
                                            cTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGt                                                     >  1:3364940/1‑101 (MQ=255)
                                             tGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTg                                                    <  2:2053915/101‑1 (MQ=255)
                                               aTCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCt                                                  <  1:1284493/101‑1 (MQ=255)
                                               aTCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCt                                                  <  2:1360877/101‑1 (MQ=255)
                                               aTCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCt                                                  <  2:925414/101‑1 (MQ=255)
                                                tCTTGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTc                                                 <  2:4209253/101‑1 (MQ=255)
                                                tCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTc                                                 <  1:2874103/101‑1 (MQ=255)
                                                tCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTc                                                 >  2:457408/1‑101 (MQ=255)
                                                 cTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCg                                                <  2:2980166/101‑1 (MQ=255)
                                                 cTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCg                                                >  2:3762501/1‑101 (MQ=255)
                                                  tGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGc                                               >  1:1111937/1‑101 (MQ=255)
                                                    gAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCt                                             <  2:1682124/101‑1 (MQ=255)
                                                       ggTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTggg                                          >  1:2072896/1‑101 (MQ=255)
                                                           gTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                      >  1:1086884/1‑101 (MQ=255)
                                                           gTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                      >  1:2331274/1‑101 (MQ=255)
                                                             gCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGGGCTCGCCTCgcaggcgg                                    >  2:4088005/1‑101 (MQ=255)
                                                               tggtggTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCt                                  <  2:225084/101‑1 (MQ=255)
                                                                 gtggtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGATCGCCTGGGCGGCGGCttt                                >  1:2497540/1‑101 (MQ=255)
                                                                 ggtgttgAAATTCTGGG.CCGCTCGAACGGGATCGG.CTTTAGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGACGGCttt                                <  2:581560/95‑1 (MQ=255)
                                                                     tCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTc                            <  1:323274/101‑1 (MQ=255)
                                                                      cGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCt                           >  1:4083966/1‑101 (MQ=255)
                                                                      cGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCt                           <  1:3008833/101‑1 (MQ=255)
                                                                       gAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTg                          >  1:2636548/1‑101 (MQ=255)
                                                                        aaTTTCT.GGTCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATTTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGc                         <  1:3252221/101‑1 (MQ=255)
                                                                          tttCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGt                       >  1:3709565/1‑101 (MQ=255)
                                                                           ttctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTg                      <  2:2542946/96‑1 (MQ=255)
                                                                             ctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGAt                    >  1:3456173/4‑101 (MQ=255)
                                                                             ctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGAt                    <  1:1149277/98‑1 (MQ=255)
                                                                             ctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGACGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGAt                    >  1:4170319/4‑101 (MQ=255)
                                                                              tggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                   <  1:727384/99‑1 (MQ=255)
                                                                              tggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                   >  1:3811418/3‑101 (MQ=255)
                                                                              tggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                   <  1:3579184/99‑1 (MQ=255)
                                                                              tggg.CAGCTCGAACGGGACCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGAGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                   >  2:250338/3‑101 (MQ=255)
                                                                                gg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCt                 <  2:2636356/101‑1 (MQ=255)
                                                                                gg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCt                 <  2:2406545/101‑1 (MQ=255)
                                                                                gg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCt                 >  2:1398858/1‑101 (MQ=255)
                                                                                gg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCt                 >  2:1982142/1‑101 (MQ=255)
                                                                                 g.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTc                <  2:2748255/101‑1 (MQ=255)
                                                                                       tCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGt           >  2:3384855/1‑101 (MQ=255)
                                                                                        cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc          >  1:420602/1‑101 (MQ=255)
                                                                                         gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg         >  1:2391529/1‑101 (MQ=255)
                                                                                         gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg         <  1:1951241/101‑1 (MQ=255)
                                                                                         gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTAGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg         <  2:1674396/101‑1 (MQ=255)
                                                                                          aaCGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGa        <  2:2850938/101‑1 (MQ=255)
                                                                                           aCGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGaa       >  1:3503688/1‑101 (MQ=255)
                                                                                            cGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAAt      <  1:1630294/101‑1 (MQ=255)
                                                                                              ggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATgg    >  2:1949846/1‑101 (MQ=255)
                                                                                              ggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATgg    <  2:2432886/101‑1 (MQ=255)
                                                                                              ggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATgg    <  1:848095/101‑1 (MQ=255)
                                                                                               gATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGc   >  1:1071913/1‑101 (MQ=255)
                                                                                                     |                                                                                                
AGCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GG.CCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCT  >  NC_014034/1786441‑1786636

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG