Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,960,668 Δ1 bp 96.4% coding (1111/1539 nt) RCAP_rcc01813 ← AMP‑dependent synthetase and ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,960,6680G→.96.4% 14.7 0.0 ‑0.7197coding (1111/1539 nt)RCAP_rcc01813AMP‑dependent synthetase and ligase
Reads supporting (aligned to +/- strand):  new base (100/90):  ref base (4/3):  total (104/93)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.06e-01

Only 100 of 210 total aligned reads displayed.

CTGATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.G.C.TCGGCGGCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCTCCGGGGGACAGGAC  >  NC_014034/1960570‑1960764
                                                                                                     |                                                                                                
 tGATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.Tcggcgcc                                                                                                >  1:2313157/1‑99 (MQ=255)
  gATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGCGCcc                                                                                               >  1:86891/1‑101 (MQ=255)
   aTAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGCGCccc                                                                                              >  2:3255275/1‑101 (MQ=255)
    tAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGCGCccca                                                                                             >  1:4088133/1‑100 (MQ=255)
     aGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCaa                                                                                           <  1:3009566/101‑1 (MQ=255)
      ggCGATGGCCCCGGCCTCGGCCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAAt                                                                                          <  2:3262730/101‑1 (MQ=255)
       gCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATa                                                                                         >  2:79118/1‑101 (MQ=255)
       gCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATa                                                                                         >  2:3034937/1‑101 (MQ=255)
         ggtGGCCCCGGCCTCGGGCATCCGCACGATGTGGCCGGTGCGGAACCAGTCCCCGGCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGc                                                                                       <  1:3745482/99‑1 (MQ=255)
           tGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCg                                                                                     <  2:2840663/101‑1 (MQ=255)
            ggCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGa                                                                                    >  1:2035197/1‑101 (MQ=255)
            ggCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGa                                                                                    <  1:3035162/101‑1 (MQ=255)
            ggCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGa                                                                                    <  1:2777259/101‑1 (MQ=255)
            ggCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGa                                                                                    <  1:3882595/101‑1 (MQ=255)
             gCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATaatcgga                                                                                   <  2:3840391/101‑7 (MQ=255)
                ccGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGaa                                                                                >  2:3537780/1‑101 (MQ=255)
                ccGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGaa                                                                                >  1:662199/1‑101 (MQ=255)
                 cGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGaaa                                                                               <  2:2801346/101‑1 (MQ=255)
                 cGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGaaa                                                                               <  1:942240/101‑1 (MQ=255)
                  ggCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGaaaa                                                                              <  2:678710/101‑1 (MQ=255)
                  ggCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGaaaa                                                                              <  1:2071756/101‑1 (MQ=255)
                  ggCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGaaaa                                                                              >  1:1112672/1‑101 (MQ=255)
                   gcgacgctCATCCGCACGATGTCGCCGGCGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAg                                                                             <  2:4107989/94‑1 (MQ=255)
                       cGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGcccc                                                                         <  2:2504995/101‑1 (MQ=255)
                        ggTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCg                                                                        >  2:2531967/1‑101 (MQ=255)
                         gTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCgg                                                                       <  1:789507/101‑1 (MQ=255)
                          tCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGa                                                                      <  1:707866/101‑1 (MQ=255)
                           cATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAACCCCGGAt                                                                     >  1:547889/1‑101 (MQ=255)
                            aTCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATc                                                                    <  2:3403366/101‑1 (MQ=255)
                            aTCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.G.C.CCCGGCGCCCCAATAACCGAGGAAAAGCCCCGGATc                                                                    >  1:1029212/1‑101 (MQ=255)
                               cGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCt                                                                 >  2:1111258/1‑101 (MQ=255)
                                gCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTg                                                                >  2:164036/1‑101 (MQ=255)
                                 cACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGc                                                               >  2:538879/1‑101 (MQ=255)
                                 cACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGc                                                               >  2:3610045/1‑101 (MQ=255)
                                   cGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCaa                                                             >  1:2861437/1‑101 (MQ=255)
                                    gATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGACCCGGATCGCTGCAAt                                                            >  1:824883/1‑101 (MQ=255)
                                      tGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.G.CTTCTGC.GCCCCAATAGCCGAGGAAAAGGCCCGGATCGCTGCAATGc                                                          >  2:3420226/1‑101 (MQ=255)
                                           ccGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGa                                                     <  1:4132450/101‑1 (MQ=255)
                                           ccGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCCCG.GCC.TCGGC.GCCCCCATAGCCGAGGAAAAGCCCCCGATCGCTGCCATGCACCGa                                                     >  1:1522725/1‑101 (MQ=255)
                                            cGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGAc                                                    >  1:2353269/1‑101 (MQ=255)
                                             gttGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACa                                                   <  2:3635241/99‑1 (MQ=255)
                                             ggTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACa                                                   <  1:118190/101‑1 (MQ=255)
                                             ggTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACa                                                   >  2:457294/1‑101 (MQ=255)
                                             ggTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACa                                                   <  1:1940527/101‑1 (MQ=255)
                                              gTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAg                                                  >  2:370215/1‑101 (MQ=255)
                                              gTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAg                                                  <  1:2241997/101‑1 (MQ=255)
                                                gCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGcg                                                >  2:1059010/1‑100 (MQ=255)
                                                gCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCt                                                <  2:2924889/101‑1 (MQ=255)
                                                 cGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTc                                               <  2:2247846/101‑1 (MQ=255)
                                                 cGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTc                                               <  1:1978255/101‑1 (MQ=255)
                                                   gAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGc                                             >  1:3461215/1‑101 (MQ=255)
                                                   gAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGc                                             >  2:187406/1‑101 (MQ=255)
                                                    aaCCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGcc                                            >  2:205404/1‑101 (MQ=255)
                                                    aaCCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCAGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGcc                                            >  2:4083482/1‑101 (MQ=255)
                                                     aCCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGccc                                           <  2:2312989/101‑1 (MQ=255)
                                                      ccAGTCCCCGTCCGCGGTGAATTTTGCCCGCCCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCg                                          <  2:652869/101‑1 (MQ=255)
                                                      ccAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCg                                          >  1:497129/1‑101 (MQ=255)
                                                        aGTCCCCGTCCGCGGGGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCCGt                                        >  2:1795339/1‑101 (MQ=255)
                                                          tCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGTC.C.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTc                                      <  1:3610298/101‑1 (MQ=255)
                                                          tCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTc                                      >  2:857904/1‑101 (MQ=255)
                                                          tCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTc                                      <  1:3255516/101‑1 (MQ=255)
                                                          tCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTc                                      <  2:1437468/101‑1 (MQ=255)
                                                           ccccGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCg                                     >  1:1060170/1‑101 (MQ=255)
                                                           ccccGTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCg                                     >  1:2534545/1‑101 (MQ=255)
                                                               gTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGcccc                                 >  2:2179507/1‑101 (MQ=255)
                                                               gTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGcccc                                 >  1:3491660/1‑101 (MQ=255)
                                                               gTCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGcccc                                 <  1:2009317/101‑1 (MQ=255)
                                                                tCCGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCg                                >  2:1955721/1‑101 (MQ=255)
                                                                 ccGCGGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCgg                               >  1:3163160/1‑101 (MQ=255)
                                                                  cgcgGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCggg                              >  2:1828262/1‑101 (MQ=255)
                                                                  cgcgGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCCCGCCCGGGTCGCCCCggg                              >  2:1499905/1‑101 (MQ=255)
                                                                   gcgGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCgggg                             >  2:2430645/1‑101 (MQ=255)
                                                                    cgGTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGc                            >  2:2979281/1‑101 (MQ=255)
                                                                     ggTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCa                           >  1:1739849/1‑101 (MQ=255)
                                                                     ggTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCa                           <  2:2861218/101‑1 (MQ=255)
                                                                      gTGAACTTTGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCAc                          >  2:2575734/1‑101 (MQ=255)
                                                                           cgttGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGAt                     <  2:3963261/99‑1 (MQ=255)
                                                                            tttGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATg                    <  1:3729512/101‑1 (MQ=255)
                                                                            tttGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATg                    >  2:1922330/1‑101 (MQ=255)
                                                                            tttGCCCGCGCCTCG.G.CATCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATg                    <  1:30069/101‑1 (MQ=255)
                                                                             ttGCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATgg                   >  2:1199702/1‑101 (MQ=255)
                                                                               gCCCGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGcc                 >  1:3998224/1‑101 (MQ=255)
                                                                                cccGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGccc                >  1:2913080/1‑100 (MQ=255)
                                                                                 ccGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGccct               >  1:3778203/1‑99 (MQ=255)
                                                                                 ccGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGcccc               >  1:2634258/1‑99 (MQ=255)
                                                                                 ccGCGCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGcccc               >  2:3087695/1‑99 (MQ=255)
                                                                                  cgcgCCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGccctc              <  2:362395/101‑4 (MQ=255)
                                                                                     gcCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCggg           <  1:2168359/101‑1 (MQ=255)
                                                                                     gcCTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCggg           >  2:1354380/1‑101 (MQ=255)
                                                                                      ccTCG.GCC.TCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCCCgggg          >  1:3301590/1‑101 (MQ=255)
                                                                                       ctcg.gcc.tcgGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCggggg         >  1:1882024/1‑101 (MQ=255)
                                                                                         tcggcc.tcggcGA.CCCAATAGCAGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGa        <  2:482736/96‑1 (MQ=255)
                                                                                         tcggcc.tcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGa        <  1:3372512/96‑1 (MQ=255)
                                                                                          cggcc.tcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGAc       <  1:368594/97‑1 (MQ=255)
                                                                                           ggcc.tcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACa      >  1:4175229/4‑101 (MQ=255)
                                                                                           ggcc.tcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACa      >  1:2262990/4‑101 (MQ=255)
                                                                                           ggcc.tcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACa      >  2:1550763/4‑101 (MQ=255)
                                                                                            gcc.tcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACAg     >  1:1632707/3‑101 (MQ=255)
                                                                                            gcc.tcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACAg     >  1:2457368/3‑101 (MQ=255)
                                                                                                tcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACAGGAc  >  1:2576546/1‑101 (MQ=255)
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CTGATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCG.G.C.TCGGCGGCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCTCCGGGGGACAGGAC  >  NC_014034/1960570‑1960764

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG