Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,123,403 Δ1 bp 96.4% coding (769/1077 nt) wza ← polysaccharide export protein Wza

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,123,4030A→.96.4% 13.6 ‑1.0 ‑1.0158coding (769/1077 nt)wzapolysaccharide export protein Wza
Reads supporting (aligned to +/- strand):  new base (81/71):  ref base (4/1):  total (85/73)
Fisher's exact test strand distribution p-value = 3.76e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.01e-01

Only 100 of 169 total aligned reads displayed.

ACGAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGA.C.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGC  >  NC_014034/2123303‑2123498
                                                                                                    |                                                                                                 
aCGAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGa                                                                                                   >  2:3597796/1‑101 (MQ=255)
 cGAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGag                                                                                                  >  2:3767012/1‑100 (MQ=255)
 cGAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGag                                                                                                  >  2:1041684/1‑100 (MQ=255)
 cGAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGag                                                                                                  >  1:3436779/1‑100 (MQ=255)
  gAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGagg                                                                                                 >  2:4000397/1‑99 (MQ=255)
  gAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGagg                                                                                                 >  2:1040317/1‑99 (MQ=255)
  gAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCAGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTAGagg                                                                                                 >  1:638103/1‑99 (MQ=255)
   aaGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGgg                                                                                                >  1:872713/1‑101 (MQ=255)
   aaGATACCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGgg                                                                                                >  2:1201863/1‑101 (MQ=255)
    aGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGggc                                                                                               >  1:1098217/1‑100 (MQ=255)
     taTGCCGCGGGCATAGGCTTTGTCCTCGGCCAGACCGCCGACGCGGGTAATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCt                                                                                             <  2:2799598/100‑1 (MQ=255)
      aTGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCtt                                                                                            >  2:3089190/1‑101 (MQ=255)
      aTGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCtt                                                                                            >  2:2443857/1‑101 (MQ=255)
       tGCCGCGGGCATCGGCCTTGTCCTCGGCCAGCCCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTg                                                                                           >  1:2386507/1‑101 (MQ=255)
       tGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGCGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTg                                                                                           <  2:1487319/101‑1 (MQ=255)
         ccGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.c                                                                                        >  1:3407953/1‑101 (MQ=255)
          cgcgGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cg                                                                                       <  1:506328/101‑2 (MQ=255)
            cgGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cgat                                                                                     >  1:3161360/1‑98 (MQ=255)
              ggCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGCTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cgatct                                                                                   <  1:3621334/101‑6 (MQ=255)
                cATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cgatctgg                                                                                 >  1:2339248/1‑94 (MQ=255)
                cATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cgatctgg                                                                                 <  2:2559740/101‑8 (MQ=255)
                  tCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cgatctggcc                                                                               >  1:1179005/1‑92 (MQ=255)
                  tCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cgatctggcc                                                                               <  2:2566675/101‑10 (MQ=255)
                  tCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cgatctggcc                                                                               <  2:1989286/101‑10 (MQ=255)
                   cGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cgatctggccc                                                                              >  2:894946/1‑91 (MQ=255)
                   cGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.cgatctggccc                                                                              >  1:2598316/1‑91 (MQ=255)
                           tCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATa                                                                      >  2:3600092/1‑101 (MQ=255)
                           tCCTCGGCCAGACCGCCGACGCGGGTGATCGACTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATa                                                                      >  1:2061258/1‑101 (MQ=255)
                            ccTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAg                                                                     <  1:3167981/101‑1 (MQ=255)
                              tCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGc                                                                   <  2:373253/101‑1 (MQ=255)
                               cGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcc                                                                  <  2:2876582/101‑1 (MQ=255)
                               cGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcc                                                                  >  2:126344/1‑101 (MQ=255)
                                ggCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcct                                                                 <  1:2776717/101‑1 (MQ=255)
                                ggCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcct                                                                 <  1:2440149/101‑1 (MQ=255)
                                 gCCTGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctc                                                                <  1:2910113/101‑1 (MQ=255)
                                 gCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctc                                                                <  2:4208559/101‑1 (MQ=255)
                                 gCCAGACCGCCGACGCGGGTGATCACCTGGGTCAGTGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctc                                                                <  2:2386331/101‑1 (MQ=255)
                                  ccAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctcc                                                               >  1:1387374/1‑101 (MQ=255)
                                  ccAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctcc                                                               >  2:1804017/1‑101 (MQ=255)
                                   cAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctcct                                                              >  2:3055976/1‑101 (MQ=255)
                                   cAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctcct                                                              <  1:933879/101‑1 (MQ=255)
                                   cAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctcct                                                              <  2:2668633/101‑1 (MQ=255)
                                    aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTg                                                             >  2:2985426/1‑101 (MQ=255)
                                       ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCAc                                                          <  1:708298/101‑1 (MQ=255)
                                         gccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCg                                                        <  2:3955401/101‑1 (MQ=255)
                                           cgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTc                                                      >  2:4114877/1‑101 (MQ=255)
                                           cgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTc                                                      <  2:2598125/101‑1 (MQ=255)
                                           cgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTc                                                      <  1:2563113/101‑1 (MQ=255)
                                           cgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTc                                                      >  2:155401/1‑101 (MQ=255)
                                              cgcgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGATG.ATTTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgc                                                   >  1:3623517/1‑101 (MQ=255)
                                              cgcgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgc                                                   <  1:1016491/101‑1 (MQ=255)
                                               gcgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgcc                                                  >  2:179472/1‑101 (MQ=255)
                                                    tGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                             <  2:2365378/101‑1 (MQ=255)
                                                    tGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                             <  1:3801051/101‑1 (MQ=255)
                                                    tGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                             <  1:108304/101‑1 (MQ=255)
                                                    tGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                             <  1:2152240/101‑1 (MQ=255)
                                                      aTCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGAc                                           >  1:3720203/1‑101 (MQ=255)
                                                      aTCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGAc                                           <  1:602162/101‑1 (MQ=255)
                                                        cGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGc                                         >  1:1854908/1‑101 (MQ=255)
                                                         gCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCt                                        <  2:3623264/101‑1 (MQ=255)
                                                         gCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCt                                        <  2:766324/101‑1 (MQ=255)
                                                          ccTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTg                                       >  2:2663724/1‑101 (MQ=255)
                                                          ccTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTg                                       <  2:1947723/101‑1 (MQ=255)
                                                           cTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTga                                      <  1:155425/101‑1 (MQ=255)
                                                           cTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTga                                      >  1:3944953/1‑101 (MQ=255)
                                                           cTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTga                                      >  1:125522/1‑101 (MQ=255)
                                                           cTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTga                                      >  1:2818084/1‑101 (MQ=255)
                                                           cTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGACAGGCCTCCTGCACCGTCTGCCGCCCGACGCTga                                      >  1:3649746/1‑101 (MQ=255)
                                                            tGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgac                                     >  2:3331902/1‑101 (MQ=255)
                                                            tGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgac                                     >  2:2063382/1‑101 (MQ=255)
                                                                tCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACa                                 >  1:763882/1‑101 (MQ=255)
                                                                 cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                <  1:3680181/101‑1 (MQ=255)
                                                                  aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                               >  1:1772331/1‑101 (MQ=255)
                                                                  aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                               >  1:3195610/1‑101 (MQ=255)
                                                                  aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                               >  2:1434798/1‑101 (MQ=255)
                                                                  aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                               <  1:1838359/101‑1 (MQ=255)
                                                                     gTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGcc                            <  2:125498/101‑1 (MQ=255)
                                                                        aTGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtt                         >  2:1592459/1‑101 (MQ=255)
                                                                          gTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                       <  1:3446718/101‑1 (MQ=255)
                                                                          gTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                       <  1:3860190/101‑1 (MQ=255)
                                                                          gTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                       >  1:2275432/1‑101 (MQ=255)
                                                                             ttCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgca                    >  2:489344/1‑101 (MQ=255)
                                                                              tCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcag                   <  1:3056201/101‑1 (MQ=255)
                                                                               ccAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagc                  <  1:179510/101‑1 (MQ=255)
                                                                               ccAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagc                  >  2:2635925/1‑101 (MQ=255)
                                                                                cAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagca                 <  1:1114624/101‑1 (MQ=255)
                                                                                cAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagca                 >  1:3854751/1‑101 (MQ=255)
                                                                                     tCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCggg            >  1:4081803/1‑101 (MQ=255)
                                                                                     tCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCggg            >  1:1413163/1‑101 (MQ=255)
                                                                                      cAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCgggc           >  1:2535253/1‑100 (MQ=255)
                                                                                       aGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgtt          <  1:2454964/101‑1 (MQ=255)
                                                                                        gatcgatcGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg         >  1:4135362/1‑101 (MQ=255)
                                                                                        gatcgatcGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg         >  2:1165923/1‑101 (MQ=255)
                                                                                         atcgatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgt        >  2:1789255/1‑101 (MQ=255)
                                                                                         atcgatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgt        >  1:396954/1‑101 (MQ=255)
                                                                                           cgatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttg      >  1:1518857/1‑101 (MQ=255)
                                                                                           cgatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttg      >  1:2792539/1‑101 (MQ=255)
                                                                                           cgatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttg      >  2:3141272/1‑101 (MQ=255)
                                                                                           cgatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGctgttg      >  2:3414463/1‑101 (MQ=255)
                                                                                           cgatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCCGCACCGTCTGCCGCCGGACGCTGACGACATCGCCGTTTTGCAGCACCGGGtggttg      >  2:4022478/1‑101 (MQ=255)
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ACGAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGA.C.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGC  >  NC_014034/2123303‑2123498

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG