Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,185,849	Δ1 bp	100%	intergenic (+122/‑383)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,185,849	0	C→.	100.0%	231.2	58	intergenic (+122/‑383)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (8/49):  ref base (0/0):  total (9/49)

ACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCG.CCCCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCCTTTCA  >  NC_014034/2185751‑2185939                                                                                                       |                                                                                            aCAGGCGGCCCCGCGCCCGCCCGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCAcc                                                                                            <  1:3208628/101‑1 (MQ=38)  cAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCAccc                                                                                           >  2:3851449/1‑101 (MQ=255)   aGGCGGCCCCGCTCCCTCCCGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCGCCG.CCCC.CGcccccc                                                                                        >  1:3454713/1‑101 (MQ=255)    ggcggcCCCGCTCCCTCCCGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCAccccc                                                                                         <  1:1271437/101‑1 (MQ=255)    ggcggcCCCGCTCCCTCCCGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCAccccc                                                                                         <  1:2741386/101‑1 (MQ=255)    ggcggcCCCGCTCCCTCCCGGGCGGGGCCG.CCGGATGTGCAAATCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCAccccc                                                                                         <  1:43915/101‑1 (MQ=37)       ggcCCCGCTCCCTCCCGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCAcccccggc                                                                                      <  1:316544/101‑4 (MQ=255)          cccCCTCCC.CCCGGGGGGGGCCGCCCTGATGTGAAACGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTa                                                                                  <  2:788581/101‑1 (MQ=18)             gCTCCCTCCCGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTAttt                                                                               <  2:2612046/101‑1 (MQ=255)             gCTCCCTCCCGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTAttt                                                                               <  1:2676140/101‑1 (MQ=255)              ctccctccCGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGCCCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTa                                                                              <  2:3466269/101‑1 (MQ=37)              ctccctccCGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGCCGGGGGGCGCCC.CCCCCCCcccccccccccgta                                                                                >  1:384193/1‑93 (MQ=255)                ccctcccGGGCGGGTCCG.GCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATc                                                                            <  2:2867915/101‑1 (MQ=25)                ccctcccGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATc                                                                            <  2:2214815/101‑1 (MQ=255)                ccctcccGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATc                                                                            <  1:1232447/101‑1 (MQ=255)                ccctcccGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATc                                                                            >  1:3516572/1‑101 (MQ=255)                 cctcccGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGCCCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATcc                                                                           <  1:3160636/101‑1 (MQ=37)                   tcccGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTTCGCCCCCGCA.CCCCCGGCGTATTTATCCCa                                                                         >  2:1658432/1‑101 (MQ=37)                   tcccGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCa                                                                         <  1:2637422/101‑1 (MQ=255)                       gggcgggGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGaa                                                                     <  1:1225076/101‑1 (MQ=255)                        ggcgggGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGaaa                                                                    <  1:4083513/101‑1 (MQ=255)                          cgggGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGaaaaa                                                                  <  2:3216645/101‑1 (MQ=255)                           ggggCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAag                                                                 <  1:3836392/101‑1 (MQ=255)                             ggCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAagca                                                               <  1:1104606/101‑1 (MQ=255)                                   cgtGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGc                                                          <  2:114422/99‑1 (MQ=37)                                   ccTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGc                                                          <  1:1768135/101‑1 (MQ=255)                                   ccTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCG.CCCCCCCACCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGc                                                          >  2:28377/1‑101 (MQ=255)                                    cTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCTGCt                                                         >  1:2199768/1‑101 (MQ=37)                                    cTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCt                                                         >  2:1406408/1‑101 (MQ=255)                                      gATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTg                                                       <  2:2666411/101‑1 (MQ=255)                                        tgtgCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGcc                                                     <  1:750529/101‑1 (MQ=255)                                          tgCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGcccc                                                   <  2:2014405/101‑1 (MQ=255)                                          tgCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGcccc                                                   <  2:1707993/101‑1 (MQ=255)                                             aaaGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGaa                                                <  1:3254556/101‑1 (MQ=255)                                             aaaGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGaa                                                <  1:4120260/101‑1 (MQ=255)                                              aaGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAg                                               <  2:2443401/101‑1 (MQ=255)                                                   gTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCtt                                          <  2:2109335/101‑1 (MQ=255)                                                    tttCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTg                                         <  2:3816667/101‑1 (MQ=255)                                                    tttCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTg                                         <  1:323867/101‑1 (MQ=255)                                                     ttCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGt                                        <  1:3739519/101‑1 (MQ=255)                                                       cGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttt                                      <  1:3825031/101‑1 (MQ=255)                                                       cGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttt                                      <  1:263307/101‑1 (MQ=255)                                                         ccTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttttg                                    >  1:710466/1‑100 (MQ=37)                                                         ccTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttttg                                    <  2:2556834/101‑2 (MQ=37)                                                           tCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCCAAGGCCTTGttttgat                                  >  2:3234517/1‑98 (MQ=25)                                                           ccggcggTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttttgat                                  <  1:845879/100‑4 (MQ=37)                                                             ggcggTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATgg                               <  1:2333974/101‑1 (MQ=37)                                                              gcggTGCCGATGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGc                              <  1:711841/101‑1 (MQ=25)                                                              gcggTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGc                              <  2:1368834/101‑1 (MQ=37)                                                              gcggTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGc                              <  2:234523/101‑1 (MQ=37)                                                                ggTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGcac                            <  2:384142/101‑1 (MQ=37)                                                                 gcgCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGcaca                           <  1:982343/99‑1 (MQ=37)                                                                 gTGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGcaca                           <  1:1961865/101‑1 (MQ=37)                                                                  tGCCGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCCAAGGCCTTG.TTTTGATGGCACaa                          >  2:529812/1‑101 (MQ=25)                                                                     ctaGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGTCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                       <  1:976740/99‑1 (MQ=25)                                                                     cGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTT.ATGGCACAAGaa                       >  2:2452572/1‑101 (MQ=37)                                                                     cGAGGCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                       <  2:4054033/101‑1 (MQ=37)                                                                       aGGCGCCCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAAc                     <  1:4104859/101‑1 (MQ=25)                                                                        ggCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACg                    <  1:1370501/101‑1 (MQ=37)                                                                        ggCGACCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACg                    <  1:3940616/101‑1 (MQ=37)                                                                           gACGCTGC..GGGGGCTCCG.CCCCCGCCCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCg                 <  1:1923837/101‑1 (MQ=37)                                                                            aCCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGa                <  2:2478842/101‑1 (MQ=37)                                                                            aCCCTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGa                <  1:4119261/101‑1 (MQ=37)                                                                              ccTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAAc              >  1:698849/1‑101 (MQ=37)                                                                              ccTGC..GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAAc              <  1:2559645/101‑1 (MQ=37)                                                                                         gCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CCCCctt    <  2:763092/101‑1 (MQ=37)                                                                                           tccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CCCCctttc  <  1:4211526/96‑3 (MQ=37)                                                                                            ccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCa  <  1:3818167/97‑1 (MQ=37)                                                                                                       |                                                                                            ACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCG.CCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCTCCG.CCCCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCCTTTCA  >  NC_014034/2185751‑2185939 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG