Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,185,902	Δ1 bp	100%	intergenic (+175/‑330)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,185,902	0	T→.	100.0%	215.7	51	intergenic (+175/‑330)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (16/35):  ref base (0/0):  total (16/35)

TTCGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCG.CCCCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGA.ACCG.CCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCGCCGGGTCTC  >  NC_014034/2185802‑2185988                                                                                                      |                                                                                          ttCGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGt                                                                                           <  1:3739519/101‑1 (MQ=255)   cGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttt                                                                                         <  1:263307/101‑1 (MQ=255)   cGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttt                                                                                         <  1:3825031/101‑1 (MQ=255)     ccTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttttg                                                                                       >  1:710466/1‑100 (MQ=37)     ccTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttttg                                                                                       <  2:2556834/101‑2 (MQ=37)       tCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCCAAGGCCTTGttttgat                                                                                     >  2:3234517/1‑98 (MQ=25)       ccggcggTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttttgat                                                                                     <  1:845879/100‑4 (MQ=37)         ggcggTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATgg                                                                                  <  1:2333974/101‑1 (MQ=37)          gcggTGCCGATGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGc                                                                                 <  1:711841/101‑1 (MQ=25)          gcggTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGc                                                                                 <  2:1368834/101‑1 (MQ=37)          gcggTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGc                                                                                 <  2:234523/101‑1 (MQ=37)            ggTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGcac                                                                               <  2:384142/101‑1 (MQ=37)             gcgCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGcaca                                                                              <  1:982343/99‑1 (MQ=37)             gTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGcaca                                                                              <  1:1961865/101‑1 (MQ=37)              tGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCCAAGGCCTTG.TTTTGATGGCACaa                                                                             >  2:529812/1‑101 (MQ=25)                 ctaGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGTCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                                                                          <  1:976740/99‑1 (MQ=25)                 cGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTT.ATGGCACAAGaa                                                                          >  2:2452572/1‑101 (MQ=37)                 cGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                                                                          <  2:4054033/101‑1 (MQ=37)                   aGGCGCCCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAAc                                                                        <  1:4104859/101‑1 (MQ=25)                    ggCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACg                                                                       <  1:3940616/101‑1 (MQ=37)                    ggCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACg                                                                       <  1:1370501/101‑1 (MQ=37)                       gACGCTGCGGGGGCTCCG.CCCCCGCCCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCg                                                                    <  1:1923837/101‑1 (MQ=37)                        aCCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGa                                                                   <  2:2478842/101‑1 (MQ=37)                        aCCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGa                                                                   <  1:4119261/101‑1 (MQ=37)                          ccTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.Ac                                                                <  1:2559645/101‑1 (MQ=37)                          ccTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.Ac                                                                >  1:698849/1‑101 (MQ=37)                                   gCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCG.CCC.Cctt                                                     <  2:763092/101‑1 (MQ=37)                                     tccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCG.CCC.Cctttc                                                   <  1:4211526/96‑3 (MQ=37)                                      ccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCa                                                   <  1:3818167/97‑1 (MQ=37)                                         gcccccgcgcccccGGCGTATTTCTCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCAAAAGAAACGCCGA.GCCGCCCC.CTTTCAcc                                                 <  1:4063879/92‑1 (MQ=255)                                               gcacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCt                                           <  1:74305/98‑1 (MQ=38)                                               gcacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCACCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCt                                           >  2:33043/4‑101 (MQ=255)                                                cacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTc                                          <  2:1691676/99‑1 (MQ=39)                                                  cccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCaa                                        >  2:69951/1‑101 (MQ=255)                                                    cccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAat                                      <  1:1406787/101‑1 (MQ=255)                                                     ccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAata                                     >  2:3229616/1‑101 (MQ=255)                                                     ccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAata                                     <  1:3834752/101‑1 (MQ=255)                                                      cgacGTATTGATCCAAAGAAACACCCGCGGCTTGCTCCGTAGACCTTG.TTTTGATGGCGCAAGAAACGCCTA.CCCT.CCCTCTTTCCCCTTGGCTCAAatat                                    <  2:1928571/98‑1 (MQ=255)                                                        gCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATcc                                  >  2:3922031/1‑101 (MQ=255)                                                        gCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATcc                                  >  2:2365467/1‑101 (MQ=255)                                                          gTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCg                                >  1:2431908/1‑101 (MQ=255)                                                           tATTTATCCCAAGGAAAAGCAGCGGCTTGCCCCGAAAGACGTG.ATTTGATGGGAGAAGAAACACCGA.ACCGACGC.CTTTCAACCTTGATCAAATATGCCgg                               >  2:1507383/1‑101 (MQ=255)                                                              ttATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGC.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCTggggg                            >  2:2839074/1‑101 (MQ=255)                                                               tATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCCAATATCCCGGGGGt                           >  1:3550084/1‑101 (MQ=255)                                                                aTCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTc                          <  2:3053969/101‑1 (MQ=255)                                                                 tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTcc                         <  1:3797515/101‑1 (MQ=255)                                                                 tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTcc                         <  1:2956496/101‑1 (MQ=255)                                                                  cccAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCg                        <  1:2502815/101‑1 (MQ=255)                                                                    cAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCggg                      <  1:114148/101‑1 (MQ=255)                                                                     aaGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCgggg                     >  1:959075/1‑101 (MQ=255)                                                                     aaGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCgcgg                     <  1:2419760/101‑1 (MQ=255)                                                                       gAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGc                   >  2:3914541/1‑101 (MQ=255)                                                                        aaaaaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCg                  <  1:3792130/101‑1 (MQ=255)                                                                         aaaaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCgg                 >  1:445142/1‑101 (MQ=255)                                                                            agcagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGc              <  1:1406507/101‑1 (MQ=255)                                                                             gcagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcc             >  2:3208555/1‑101 (MQ=255)                                                                             gcagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcc             >  2:3610289/1‑101 (MQ=255)                                                                             gcagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTATCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAgac             >  2:4206718/1‑99 (MQ=37)                                                                               agcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccc           <  1:3234756/101‑2 (MQ=37)                                                                                gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGccccc          <  2:3454461/101‑3 (MQ=37)                                                                                gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGccccc          <  2:958990/101‑3 (MQ=37)                                                                                gcGGCTTCCCCCGAAGGCTTTG.TTTTGATGGCAAAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGccccc          <  2:1504252/101‑3 (MQ=25)                                                                                    cTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccggcg      >  2:401625/1‑95 (MQ=25)                                                                                        cccgGAGGGCCTTG.TTTTGACGGCACAAGAAACGCCGACCCCC.CCC.CTTTCCCCTTGGCTCAAATTCCCCGGGGGTCGGGGGGGGGAGcccccggcgggtc  <  2:1067302/101‑11 (MQ=9)                                                                                                      |                                                                                          TTCGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCG.CCCCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGA.ACCG.CCC.CTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCGCCGGGTCTC  >  NC_014034/2185802‑2185988 Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG