Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,435,309 Δ1 bp 4.1% intergenic (+18/‑36) RCAP_rcc02259 → / → RCAP_rcc02260 WbqC‑like protein family/type 11 family methyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,435,3090C→. 4.1% 14.2 ‑0.3 ‑0.2172intergenic (+18/‑36)RCAP_rcc02259/RCAP_rcc02260WbqC‑like protein family/type 11 family methyltransferase
Reads supporting (aligned to +/- strand):  new base (5/2):  ref base (98/65):  total (105/67)
Fisher's exact test strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.73e-01

Only 100 of 218 total aligned reads displayed.

ATACAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGCCATGC  >  NC_014034/2435209‑2435409
                                                                                                      |                                                                                                    
ataCAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.c                                                                                                      <  1:3564469/101‑1 (MQ=255)
 taCAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.cc                                                                                                     >  2:217077/1‑101 (MQ=255)
  aCAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.ccc                                                                                                    >  1:3906390/1‑101 (MQ=255)
  aCAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.ccc                                                                                                    >  2:1312905/1‑101 (MQ=255)
     ggAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.ctcccc                                                                                                 >  2:3781003/1‑101 (MQ=255)
     ggAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.cccccc                                                                                                 <  2:1961970/101‑1 (MQ=255)
       aaaaCATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.cccccccc                                                                                               <  1:4030182/101‑1 (MQ=255)
        aaaCATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGGGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.ccccccccc                                                                                              >  1:2260315/1‑101 (MQ=255)
        aaaCATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.ccccccccc                                                                                              >  1:1464439/1‑101 (MQ=255)
        aaaCATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.ccccccccc                                                                                              <  1:3288113/101‑1 (MQ=255)
         aaCATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.cccccccccc                                                                                             >  1:2976204/1‑101 (MQ=255)
         aaCATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.cccccccccc                                                                                             <  1:511849/101‑1 (MQ=255)
          acatacatGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCg                                                                                            <  1:2706430/101‑1 (MQ=255)
          acatacatGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCg                                                                                            <  2:1507815/101‑1 (MQ=255)
          acatacatGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAAGCTGACCCTT.CCGATCCGTGTG.ccccccccccc                                                                                            >  1:3612591/1‑100 (MQ=255)
            atgcatGTTTTCTTCCCCCCCGGTTCGAACGGCCCGTGGGGGCGTTGCGTGCACGCATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGaa                                                                                          <  2:2366539/98‑1 (MQ=255)
            atacatGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGaa                                                                                          >  1:425616/1‑101 (MQ=255)
              acatGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGaaaa                                                                                        >  1:1698865/1‑101 (MQ=255)
              acatGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGaaaa                                                                                        >  1:247454/1‑101 (MQ=255)
              acatGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.ACCCCCCCCCGaaaa                                                                                        >  2:3665555/1‑101 (MQ=255)
               catGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAAt                                                                                       <  1:3728116/101‑1 (MQ=255)
                 tGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATcc                                                                                     >  1:1347613/1‑101 (MQ=255)
                     ttCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATcccccg                                                                                 >  2:365023/1‑99 (MQ=255)
                       cTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCCAAAATCCCCCAGc                                                                               >  1:1795587/1‑101 (MQ=255)
                        cgcccgcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCg                                                                              <  2:278334/100‑1 (MQ=255)
                         gcccgcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTAACCCTT.CCGATCCGTGTG.CCCCCCCCCAAAAAATCCCCGAGCgg                                                                             <  2:2250366/101‑1 (MQ=255)
                            cgcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGgaga                                                                          <  1:2110307/101‑1 (MQ=255)
                            cgcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG..CCCCCCCCCGAAAATCCCCGAGCGGAGaa                                                                         >  1:3755060/1‑101 (MQ=255)
                             gcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAAACCCCGAGCTGGGaa                                                                         >  2:1246837/1‑101 (MQ=255)
                             gcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCCCAAATCCCCGCGCGGCGaa                                                                         >  1:3704270/1‑101 (MQ=255)
                              cccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAg                                                                        <  1:2608350/101‑1 (MQ=255)
                              cccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGACCGGAGAAg                                                                        >  2:1093570/1‑101 (MQ=255)
                                 tGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCAAGCGGGGAACCgc                                                                     >  1:4108021/1‑100 (MQ=255)
                                 tGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG..CCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTc                                                                    >  2:345066/1‑101 (MQ=255)
                                  gTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGACGCCTc                                                                    >  2:419542/1‑101 (MQ=255)
                                   ttCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTcc                                                                   >  2:2470602/1‑101 (MQ=255)
                                   ttCGAACGGACCGTGGCGGAGTGGCGTGCAGACATCTTGGAAGGCCGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTcc                                                                   <  2:3947115/101‑1 (MQ=255)
                                    tCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGCCCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAATTCCCCGACCGGAGAAGGGTCCa                                                                  >  2:1669308/1‑101 (MQ=255)
                                    tCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCa                                                                  <  2:2848578/101‑1 (MQ=255)
                                    tCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCCAAAATCCCCCGGGCGAGCAGCGTCCa                                                                  >  1:92112/1‑101 (MQ=255)
                                     cGAACGGCCCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCAt                                                                 <  2:1451364/101‑1 (MQ=255)
                                      gAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATg                                                                <  1:4022334/101‑1 (MQ=255)
                                      gAACGGACCGTGGCGGAGTTGCGTGCAAACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATg                                                                <  2:2495966/101‑1 (MQ=255)
                                       aaCGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAACCGTCAATgg                                                               >  2:3354975/1‑101 (MQ=255)
                                         cGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTGCCCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGCCCATGGc                                                              >  1:3433590/1‑101 (MQ=255)
                                         cGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCCAAAATCCCCGAGCGGAGAAGCGTCCAtaccc                                                             >  1:3476933/1‑97 (MQ=255)
                                            aCCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCa                                                          <  2:3518785/101‑1 (MQ=255)
                                             ccGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCaa                                                         >  2:1168612/1‑101 (MQ=255)
                                               gggggggggTTGGGCGCAAACATCTTGAAAGGGCGGCCCTT.CCTATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGc                                                       <  1:1312945/93‑1 (MQ=255)
                                                tGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCg                                                      >  1:2310657/1‑101 (MQ=255)
                                                  gCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGAc                                                    <  1:3980818/101‑1 (MQ=255)
                                                     gAGTTGCGTGCAGACATCTTGGAAGGCTGCCCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGAcccc                                                 <  1:310131/101‑1 (MQ=255)
                                                      aGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCACGAGGGGGGAAGCGCCCATGGCCCCAAACGACCCCg                                                >  1:588063/1‑101 (MQ=255)
                                                       gTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCgg                                               <  2:242402/101‑1 (MQ=255)
                                                       gTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGGCCATGGCCCCAAGCGACCCCgg                                               >  1:292640/1‑101 (MQ=255)
                                                        ttGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGt                                              <  2:20607/101‑1 (MQ=255)
                                                        ttGCGGGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGt                                              <  1:2730915/101‑1 (MQ=255)
                                                           ggTGCAGACTTCTGGGAAGGCTGCCCCTT.TCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGac                                           <  1:2513239/100‑1 (MQ=255)
                                                            gTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGGGacc                                          >  1:1664624/1‑100 (MQ=255)
                                                              gCAGACATTTGGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACAcc                                        <  2:4074109/101‑1 (MQ=255)
                                                              gCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG..CCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACAACg                                       >  2:3569349/1‑101 (MQ=255)
                                                               cAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCg                                       >  1:2024155/1‑101 (MQ=255)
                                                                aGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAACTCCCCGGGCGGAGAAGCGCCCATGGCCCCACGCGACCCCGGGGAAACCGt                                      >  2:1050264/1‑101 (MQ=255)
                                                                   aaTCTTGGAAGCCTGTCCCTT.CCGATCCGTGTT.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGAt                                   <  2:3124764/100‑1 (MQ=255)
                                                                    aTCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATg                                  >  1:641842/1‑101 (MQ=255)
                                                                     tCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATgc                                 <  1:2721205/101‑1 (MQ=255)
                                                                      cTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATgcc                                >  1:3990842/1‑101 (MQ=255)
                                                                      cTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATgcc                                >  2:1794469/1‑101 (MQ=255)
                                                                       ttGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATgccg                               <  2:1698750/101‑1 (MQ=255)
                                                                        tGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGGGCCCATGGCCCCAACCGACCCCGGGAACACCGTGAGgcccc                              >  2:2576682/1‑99 (MQ=255)
                                                                         ggAAGGCTGACCCTT.CCGATCCGTGTG..CCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGccgccc                            >  1:2615530/1‑101 (MQ=255)
                                                                           aaGGCTGCCCCTT.CCTATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGccgcccg                           <  2:1347513/101‑1 (MQ=255)
                                                                           aaGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGccgcccg                           >  2:488021/1‑101 (MQ=255)
                                                                           aaGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCCGTGACACCGGGATGccgcccc                           >  1:1067125/1‑100 (MQ=255)
                                                                             ggCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGCGCGGGGAAGCGTCCCTGGCCCCAAGCGACCCCGGGGACACCGTGATGCcgcccgcc                         >  1:2572642/1‑101 (MQ=255)
                                                                             ggCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCcgcccgcc                         <  2:730735/101‑1 (MQ=255)
                                                                              gCTGCCCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCt                        <  2:2310489/101‑1 (MQ=255)
                                                                               cTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCtt                       >  2:344098/1‑101 (MQ=255)
                                                                                tGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTtc                      >  1:2453071/1‑101 (MQ=255)
                                                                                 gACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTtcg                     <  2:2024017/101‑1 (MQ=255)
                                                                                 gACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTtcg                     >  2:1102094/1‑101 (MQ=255)
                                                                                  gcccTTCCCGACCCGTG.G.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTtcgt                    <  2:3251246/100‑1 (MQ=255)
                                                                                  aCCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTtcgt                    >  1:1564290/1‑101 (MQ=255)
                                                                                    ccTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTtcgtcg                  <  1:2129790/101‑1 (MQ=255)
                                                                                    ccTT.CCGATCCGTGTG.CCCCCCCCCCCAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGGGATGCCGCCCGCCTtcgacc                  >  1:2649585/1‑98 (MQ=255)
                                                                                      tt.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGCGCGGAGAAACGCCCATGGCCCCCAGAGACCCCGGGGACACCGTGATGCCGCCCGCCCtcgtagct                >  1:2201163/1‑97 (MQ=255)
                                                                                       t.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATc               >  1:1262538/1‑101 (MQ=255)
                                                                                         ccGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGGGACACCGCGATGCCGCCCGCCTTCGTCGATcc              >  1:3064281/1‑101 (MQ=255)
                                                                                           gATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGGGACACCGTGATGCCGCCCGCCTTCGTCGACCCGc            >  1:98627/1‑101 (MQ=255)
                                                                                           gATCCGTGTG.CCCCCCCCCC.AAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGcc           >  2:1611766/1‑100 (MQ=255)
                                                                                             tCCGTGTC.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTc          <  2:3906131/101‑1 (MQ=255)
                                                                                              ccGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCg         <  2:3500965/101‑1 (MQ=255)
                                                                                                gtgtG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGc       >  2:589392/1‑101 (MQ=255)
                                                                                                gtgtG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGc       >  1:1243617/1‑101 (MQ=255)
                                                                                                gtgtG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCCGGATGCCGCCCGCCTTCGTCGATCCGCTCGGc       >  2:2216460/1‑101 (MQ=255)
                                                                                                gtgtG.CCCCCCCCCCGAAAATACCCGAGCGGGGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGCTGCCCCCCGCCTTCGTCGATCCGCCCGGc       >  1:2407437/1‑101 (MQ=255)
                                                                                                 tgtg.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGcc      <  1:810439/101‑1 (MQ=255)
                                                                                                  gtg.CCCCCCCCCCGAAAAACCCCCAGCGGAGAAAGGTCCATGGCCCCAAGCGGCCCCGGGGACCACGGGATGCCGCCCGCCTTCGCCGATCAGCTCGCccc     >  2:26972/1‑100 (MQ=255)
                                                                                                      ccccccccccGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGCCATgc  <  1:480399/101‑1 (MQ=255)
                                                                                                      cccccccaccGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACGCCGTGATGCCGCCCGCCTTGGTCGATCCGCTCCGctctgc  <  2:140316/101‑6 (MQ=255)
                                                                                                      |                                                                                                    
ATACAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTT.CCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGCCATGC  >  NC_014034/2435209‑2435409

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG