Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,474,914	Δ1 bp	100%	intergenic (‑77/+49)	RCAP_rcc02301 ← / ← engA	hypothetical protein/GTP‑binding protein EngA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,474,914	0	G→.	100.0%	88.4	27	intergenic (‑77/+49)	RCAP_rcc02301/engA	hypothetical protein/GTP‑binding protein EngA
Reads supporting (aligned to +/- strand):  new base (22/5):  ref base (0/0):  total (22/5)

GCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGGCGCAGCCCCCC..GCGGGTCGCCTCGGGCTTGCCCGAGGCGA.CATCA.G.CCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTTGCGTGCGGCGT  >  NC_014034/2474814‑2475009                                                                                                     |                                                                                                     gCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCg                                                                                                      <  2:1484612/101‑1 (MQ=255)  cTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgg                                                                                                     <  1:662834/101‑1 (MQ=255)    cccccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCcggg                                                                                                   <  1:3260323/101‑1 (MQ=255)     ccccGGGATATTTCCCCCAAGGTGACAGGAATCCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATGCGCAAGGCGGGGGGTGCggggg                                                                                                  <  1:4189680/101‑1 (MQ=255)     ccccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggg                                                                                                  <  2:4055930/101‑1 (MQ=255)      cccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgggggg                                                                                                 <  1:533064/101‑1 (MQ=255)       ccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggc                                                                                                <  2:3257938/101‑2 (MQ=255)        cGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcg                                                                                               <  1:3797785/101‑3 (MQ=255)        cGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCCTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcg                                                                                               >  2:4212705/1‑99 (MQ=255)         gggATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcgc                                                                                              <  2:562990/101‑4 (MQ=255)         gggATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcgc                                                                                              <  1:3313238/101‑4 (MQ=255)          ggATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcgca                                                                                             <  2:1302872/101‑5 (MQ=255)           gATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAg                                                                                           <  2:2951742/101‑1 (MQ=255)            atatTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGc                                                                                          >  1:2096954/1‑101 (MQ=255)             tatTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcc                                                                                         <  2:2867226/101‑1 (MQ=255)              atTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccc                                                                                        <  2:1557884/101‑1 (MQ=255)               tttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACCCAAGGCGGGGGGGGCggggggggccggccc                                                                                        >  2:1748053/1‑93 (MQ=255)                tccccccAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccccc                                                                                      <  1:110821/99‑1 (MQ=255)                 tCCCCCAAGGTGACAGGAACCCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccccc                                                                                     <  1:1647055/101‑1 (MQ=255)                  cccccAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCc..g                                                                                  >  1:1900282/1‑101 (MQ=255)                            gACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCC..GCgggcccccc                                                                        >  1:140959/1‑95 (MQ=255)                            gACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCC..GCGGGGCGccc                                                                        >  1:191671/1‑100 (MQ=255)                             aCAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGGGC.GGGGGGCGCAGCCCCCC..GCGGGGCGcccc                                                                       >  1:1966771/1‑99 (MQ=39)                                  aacccGCGGCCCCCCCCGCCCGAACGGCCCGTCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGTCGCAGCCCCCA..GCGGGTCGCCTCGGGCt                                                                  <  1:3072609/98‑1 (MQ=25)                                  aaaCCGCGGCCCCCCCCCCCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAACGCGGGGGCCGC.GGGGGGCGCGGCCCCCC..GCGCGTCGCCCCCGGcc                                                                  >  2:2019823/1‑100 (MQ=255)                                  aaaCCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCC..GCGGGGCGCCCCCGGcg                                                                  >  2:3423260/1‑100 (MQ=37)                                                                 gCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCC..GCGGGGCGCCCCCGGCTTGGCCCGGGGGG.CATCC.G.GCCCTCGGtgggg                                >  1:988341/1‑97 (MQ=12)                                                                  cTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCC..GCGGGTAGCCTCGGGCTTGCCCGGGGCGG.CAACA.GACCCTCCGGttcta                                >  2:304394/1‑98 (MQ=18)                                                                   ttttCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCC..GCGGGGCGCCTCGGGCTTGCCCGAGGCGA.CATCA.G.CCCCTCCGGTTCGAg                              >  2:3963072/1‑101 (MQ=21)                                                                   ttttCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCC..GCGGGGCGCCCCGGGCTTGCCCCCGGGGG.CATCC.G.CCCCTCGGGTTCgcg                              >  1:2250578/1‑99 (MQ=18)                                                                     ttCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCC..GCGGGGCGCCTCGGGGTTGCCCCAGGGGG.CATCA.G.CCCCTCCGGTTctgggg                            >  2:1507325/1‑96 (MQ=18)                                                                           ggCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCC..GCGGGTCGCCCCGGGCGTGGCCCAGGGGACCACCA.C.CCCTCCGGTTTccgggccagcc                       >  1:3974852/1‑91 (MQ=12)                                                                              cAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCC..GCGGGTCGCCTCGGGCTTGCCCGAGGCGA.CATCA.G.CCCTTCGGTTTCGAGGGCCGGGCCtt                   >  2:3969989/1‑101 (MQ=255)                                                                                 aTACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCC..GCGGGGCGCCCCGGGCTTGCCCGGGGGGA.CATCA.G.CCCTTCGGTTTCGAGGGCAGGCCCTTGTc                >  1:3073577/1‑101 (MQ=25)                                                                                 aTACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCC..GCGGGCCGCCTCCGGCTTGCCCGCGGCGA.GAGCA.C.CCCCCCGGCTTCGAGGGCGGGCCCCTGTc                >  1:4175514/1‑101 (MQ=12)                                                                                    cGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCC..GCGGGGCGCCCCGGGGCTGCCCCCGGCGG.CAACA.G.CCCCTCCGGTTTGAGGGGAGGGCCCTGTccct             >  2:1450068/1‑99 (MQ=9)                                                                                       aaGGCGGGGGGTGC.GGGGGGCGCAGCCCCCC..GCGGGGCGCCCCCGGGTTGCCCGCGGGGG.CCCCC.G.CCCCTCGGGTTCGCGGGCCGGGCCCTGGCCCtgcg          >  1:408598/1‑101 (MQ=9)                                                                                         gggggggggGGGGGGGGGGCCCCCCCCCCCGGGCGGGTCGCCTCGGGCTTGCCCGAGGCGA.CATCA.G.CCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTtgc           <  1:3423505/98‑1 (MQ=25)                                                                                            ggggggTGC.GGGGGGCGCAGCCCCCC..GCGGGTCGCCTCGGGCTTGGCCGAGGCGA.CATCA.G.GCCTTCGGTTTCGAGGGGCGGGCCCTGTCCTTCCGTgcgg     >  2:2367299/1‑101 (MQ=18)                                                                                            ggggggTGC.GGGGGGCGCAGCCCCCC..GCGGGTCGCCTCGGGCTTGCCCGAGGCGA.CATCA.G.CCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTTTCGTgcgg     >  2:2628960/1‑101 (MQ=255)                                                                                             gggggTGC.GGGGGGCGCAGCCCCCC..GCGGGGCGCCCCCGGGTTGCCCCGGGGGG.CCTCAGG.CCCTCGGGTTTGGGGGCCGGGCCCTTTCCCTTGC.Tgcggg    >  1:3307961/1‑100 (MQ=2)                                                                                              ggggTGC.GGGGGGCGCAGCCCCCC..GCGGGTCGCCCCCGGCGTGCCCGCGGGGG.CAACA.C.CCCCTCGGTTTTTAGGGGAGGCCCCCGTCCTTGCGTgcggcg   >  1:2226798/1‑101 (MQ=12)                                                                                               gggTGC.GGGGGGCGCAGCCCCCC..GCGGGGCGCCTCGGGGTTGCCCGGGGGGG.CATCA.G.CCCCTCGGTTTCGAGGGGAGGGCCCTGTCCCTGCGGgcggggg  >  1:764335/1‑98 (MQ=255)                                                                                                     |                                                                                                     GCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGGCGCAGCCCCCC..GCGGGTCGCCTCGGGCTTGCCCGAGGCGA.CATCA.G.CCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTTGCGTGCGGCGT  >  NC_014034/2474814‑2475009 Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG