Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,022 Δ1 bp 7.0% intergenic (+70/‑172) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0220C→. 7.0% 25.4 ‑0.7 ‑1.3143intergenic (+70/‑172)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/4):  ref base (59/72):  total (67/76)
Fisher's exact test strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.78e-02

Only 100 of 154 total aligned reads displayed.

GGCGGGCGCGCGGCACGACAGGCCGAGTAA..GGG.GGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGG  >  NC_014034/2493923‑2494121
                                                                                                      |                                                                                                        
  cgggcgCGCGGCACGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                                        >  2:1314684/1‑101 (MQ=21)
   gggcgCGCGGCACGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                       <  2:1517101/101‑1 (MQ=21)
   gggcgCGCGGCACGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCCGGCTCCCCCCGGGATATTTGCAGCAAGAGGAAACCGGATGCCtt                                                                                                       >  2:1852319/1‑101 (MQ=21)
    ggcgCGCGGCACGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                      <  2:3798128/101‑1 (MQ=21)
    ggcgCGCGGCACGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                      <  2:2677322/101‑1 (MQ=21)
     gcgcgcGGCACGACAGGCCGAGGAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                     <  2:1972186/101‑1 (MQ=18)
      ggcgcgGCACGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                    <  2:1565830/100‑1 (MQ=18)
      cgcgcgGCACGACAGGCCGAGTAAGGGGG.GGGGCGCTGCCCCCCGCCCGCAAGCGGACTCCCCCCGGGAATTTTGCAGCAAAATGAAACCGGAGGCCTTTc                                                                                                     >  1:1059554/1‑101 (MQ=18)
      cgcgcgGCACGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                    <  1:3896555/101‑1 (MQ=21)
      cccgcgGCACGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                    <  1:1388558/99‑1 (MQ=18)
       gcgcgGCACGACAGTCCGAGTAA..GGGCGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                                                   <  2:2808832/101‑1 (MQ=18)
       gcgcgGCACGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGACTTTCAt                                                                                                   >  2:734027/1‑101 (MQ=18)
         gcgGCACGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                                 <  2:1549294/101‑1 (MQ=21)
         gcgGCACGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                                 >  1:3138380/1‑101 (MQ=21)
          cggccggACAGGCCGAGTAAG.GGG.GGGGCCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                                <  1:2413190/95‑1 (MQ=18)
          cgGCACGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                                <  1:134877/101‑1 (MQ=21)
            gCACGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGc                                                                                              >  2:1165122/1‑101 (MQ=21)
             cACGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                                             >  1:1014986/1‑101 (MQ=21)
              aCGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGGCTTTCATCTTGccc                                                                                            >  2:3040367/1‑101 (MQ=18)
              aCGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                            <  2:2354520/101‑1 (MQ=21)
               cGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                           >  1:1888872/1‑101 (MQ=21)
               cGACAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGAGATTTGCAGCAAGATGAAACCGGACGCCTTTCATCTTGCCCg                                                                                           >  1:1524658/1‑101 (MQ=18)
                  cAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                        <  2:3183986/101‑1 (MQ=21)
                  cAGGCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                        >  2:3814169/1‑101 (MQ=21)
                    ggCCGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                      <  1:3201927/101‑1 (MQ=21)
                       cGAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                   <  1:746828/101‑1 (MQ=21)
                        gAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                  <  1:275202/101‑1 (MQ=18)
                        gAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                  >  2:3928627/1‑101 (MQ=18)
                        gAGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAACGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                  >  1:3947124/1‑101 (MQ=18)
                         aGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                 <  1:1137456/101‑1 (MQ=18)
                         aGTAAG.GGG.GGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                 <  1:2718827/101‑1 (MQ=18)
                          aggaagggg.ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                 <  2:177793/95‑1 (MQ=18)
                           gtaagggg.ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                <  1:41580/96‑1 (MQ=255)
                            taagggg.ggggCCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGCGATATTTGCAGCAAGATGAAACCCGATGCCTGTCATCTTTGCCGAAACATCCCcgg                                                                               >  2:2503285/5‑101 (MQ=14)
                            aaggggg.ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                               <  1:3531791/97‑1 (MQ=255)
                             aagggg.ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                              <  1:2488527/98‑1 (MQ=255)
                               gggg.ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                            >  1:2602730/2‑101 (MQ=255)
                                ggg.ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAATATCCCggggggg                                                                           >  2:1224466/1‑100 (MQ=255)
                                ggg.ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggggg                                                                           >  1:2476508/1‑100 (MQ=255)
                                ggg.ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                           >  1:1407280/1‑101 (MQ=255)
                                 gg.ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCcgggggcg                                                                          >  1:4000270/1‑99 (MQ=255)
                                  g.ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                         >  1:2113492/1‑101 (MQ=255)
                                     gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                      >  1:2855095/1‑101 (MQ=255)
                                      ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                     >  1:1980829/1‑101 (MQ=255)
                                          cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                              >  2:3680676/1‑101 (MQ=255)
                                           tccccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                             <  1:1852439/99‑2 (MQ=255)
                                           tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGCA.TGGC..G.aa                                                             >  1:123812/1‑100 (MQ=255)
                                           tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aa                                                             >  1:3321008/1‑100 (MQ=255)
                                            gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                            <  2:4031543/101‑3 (MQ=255)
                                            gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                            >  1:301015/1‑99 (MQ=255)
                                            gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aag                                                            >  1:703809/1‑99 (MQ=255)
                                             cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                          <  1:157847/101‑5 (MQ=255)
                                             cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATCCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGGC.TGGC..G.aagcc                                                          >  2:2595949/1‑97 (MQ=18)
                                              ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagcca                                                         >  1:3490525/1‑96 (MQ=18)
                                                cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                        <  1:1478641/97‑7 (MQ=255)
                                                cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                        <  1:2631089/97‑7 (MQ=255)
                                                 ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                       >  2:3182049/4‑94 (MQ=255)
                                                   gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                     <  2:4026053/100‑10 (MQ=255)
                                                            cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcg                                             >  1:262851/1‑98 (MQ=18)
                                                             ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcgc                                            >  2:4233939/1‑97 (MQ=18)
                                                              gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cc                                         >  2:3495375/1‑101 (MQ=17)
                                                               aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.ccc                                        >  2:181846/1‑101 (MQ=17)
                                                                cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccc                                       <  1:2553565/101‑1 (MQ=17)
                                                                 tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.ccccc                                      >  2:633975/1‑101 (MQ=17)
                                                                   cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccct                                    <  2:936538/101‑3 (MQ=17)
                                                                   cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccct                                    <  1:4113550/101‑3 (MQ=17)
                                                                   cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.acccccg                                    >  2:2285455/1‑99 (MQ=17)
                                                                     cccGGGATAGTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccctgc                                  <  2:2113354/101‑5 (MQ=17)
                                                                        gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGcccc                                <  2:3255155/101‑1 (MQ=17)
                                                                        gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGcccc                                <  2:3008822/101‑1 (MQ=17)
                                                                         ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcg                               >  1:176553/1‑101 (MQ=2)
                                                                          gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgc                              <  1:76569/101‑2 (MQ=0)
                                                                           atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgcc                             <  1:2038261/101‑3 (MQ=2)
                                                                           atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgcc                             >  2:2911175/1‑99 (MQ=2)
                                                                             atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                           >  1:3984679/1‑101 (MQ=17)
                                                                               ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgccggat                         <  1:582313/101‑3 (MQ=2)
                                                                                tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                        >  1:3235319/1‑101 (MQ=17)
                                                                                 gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCATCCC.CCCCCTGCCCCGGCGGTCCg                       <  2:3138149/101‑1 (MQ=18)
                                                                                 gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                       <  1:3695775/101‑1 (MQ=17)
                                                                                   agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg                     <  2:1717607/101‑1 (MQ=17)
                                                                                   agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg                     <  1:181884/101‑1 (MQ=17)
                                                                                    gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTgg                    <  2:1059460/101‑1 (MQ=17)
                                                                                     caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGGCCGTggg                   >  1:143678/1‑100 (MQ=17)
                                                                                       aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                 >  2:625069/1‑101 (MQ=18)
                                                                                       aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                 <  1:2538613/101‑1 (MQ=17)
                                                                                         aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCAGCTC.CCCCCTGCCCCGGCGGTCCGTGgcggcg               >  1:2845295/1‑101 (MQ=18)
                                                                                           gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGGGGCGcc             >  2:1265273/1‑101 (MQ=17)
                                                                                           gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TAGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc             >  1:335947/1‑101 (MQ=17)
                                                                                            aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCt            >  2:911065/1‑101 (MQ=17)
                                                                                            aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCt            <  1:3680930/101‑1 (MQ=17)
                                                                                            aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGGCCGGGGCGGCGGCt            >  1:2207444/1‑101 (MQ=9)
                                                                                              aCCGGATGCCTTCCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCAGGCCGTGGGGGGGCCTTg          >  1:932663/1‑101 (MQ=9)
                                                                                                cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc        <  1:882183/101‑1 (MQ=17)
                                                                                                cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCAGAGGGGGGCAGGGC.CCCCCTGCCCCGGCGGGCCGCGGCGGCGCCCTGAc        >  1:3622924/1‑101 (MQ=17)
                                                                                                  gATGTCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCGGAGGGGGGCAGGGC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg      <  2:1353657/101‑1 (MQ=18)
                                                                                                  gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg      <  2:2298375/101‑1 (MQ=17)
                                                                                                   aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg     <  1:4122895/101‑1 (MQ=17)
                                                                                                    tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc    <  2:1784540/101‑1 (MQ=17)
                                                                                                     gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg   <  2:2046365/101‑1 (MQ=17)
                                                                                                      ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg  <  1:1165216/101‑1 (MQ=17)
                                                                                                      |                                                                                                        
GGCGGGCGCGCGGCACGACAGGCCGAGTAA..GGG.GGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGG  >  NC_014034/2493923‑2494121

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG