Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,047 Δ1 bp 8.3% intergenic (+95/‑147) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0470G→. 8.3% 24.6 ‑0.3 ‑0.4121intergenic (+95/‑147)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/4):  ref base (58/53):  total (64/57)
Fisher's exact test strand distribution p-value = 7.48e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.42e-01

Only 100 of 143 total aligned reads displayed.

AGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGC  >  NC_014034/2493948‑2494146
                                                                                                    |                                                                                                           
aggaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                                             <  2:177793/95‑1 (MQ=18)
aGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                                             <  1:2718827/101‑1 (MQ=18)
aGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                                             <  1:1137456/101‑1 (MQ=18)
 gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                                            <  1:41580/96‑1 (MQ=255)
  taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                                           >  1:1814213/5‑101 (MQ=255)
  taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGAGATTTGCAGCACGATGAAACCGGATGCCTTTCATATTGCCCGCAATATCCCcgg                                                                                                           >  2:2879137/5‑101 (MQ=18)
   aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                                                          <  1:2488527/98‑1 (MQ=255)
    aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                                                         >  2:1781926/3‑101 (MQ=255)
     ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                                                        >  1:2602730/2‑101 (MQ=255)
      gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAATATCCCggggggg                                                                                                       >  2:1224466/1‑100 (MQ=255)
      gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggggg                                                                                                       >  1:2476508/1‑100 (MQ=255)
      gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                                                       <  2:3679823/101‑1 (MQ=255)
       ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCcgggggcg                                                                                                      >  1:4000270/1‑99 (MQ=255)
        gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                                                     >  1:2113492/1‑101 (MQ=255)
         ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                                                    <  1:3964032/101‑1 (MQ=255)
          gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                  >  1:2855095/1‑101 (MQ=255)
          gcgcccTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                  <  2:915472/96‑1 (MQ=255)
             cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                                               <  1:4123983/101‑1 (MQ=255)
               cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                                          >  2:3680676/1‑101 (MQ=255)
                tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGCA.TGGC..G.aa                                                                                         >  1:123812/1‑100 (MQ=255)
                 gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                        <  2:4031543/101‑3 (MQ=255)
                 gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                        >  1:301015/1‑99 (MQ=255)
                 gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aag                                                                                        >  1:703809/1‑99 (MQ=255)
                  cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                      <  1:157847/101‑5 (MQ=255)
                  cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATCCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGGC.TGGC..G.aagcc                                                                                      >  2:2595949/1‑97 (MQ=18)
                  cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                                                       <  2:1014895/101‑4 (MQ=255)
                   ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagcca                                                                                     >  1:3490525/1‑96 (MQ=18)
                     cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                    <  1:2631089/97‑7 (MQ=255)
                      ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                   >  2:3182049/4‑94 (MQ=255)
                       cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                                                  <  1:4031167/99‑9 (MQ=255)
                        gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                                                 <  2:4026053/100‑10 (MQ=255)
                        gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                                                 >  2:1266907/2‑92 (MQ=255)
                                  ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAGGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGCaccgc                                                                      >  1:1861491/1‑97 (MQ=17)
                                    aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.ccc                                                                  >  2:181846/1‑101 (MQ=17)
                                     cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccc                                                                 <  1:2553565/101‑1 (MQ=17)
                                     cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccc                                                                 >  2:231394/1‑101 (MQ=18)
                                     cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccc                                                                 <  2:1471921/101‑1 (MQ=17)
                                      tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.ccccc                                                                >  2:321368/1‑101 (MQ=18)
                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccccct                                                              <  2:936538/101‑3 (MQ=17)
                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccccct                                                              <  2:640139/101‑3 (MQ=17)
                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccccct                                                              >  2:3155734/1‑99 (MQ=17)
                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.acccccg                                                              >  2:2285455/1‑99 (MQ=17)
                                          cccGGGATAGTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccccctgc                                                            <  2:2113354/101‑5 (MQ=17)
                                            cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGA..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGccc                                                           <  1:3748899/101‑1 (MQ=17)
                                             gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGcccc                                                          <  2:3255155/101‑1 (MQ=17)
                                             gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGcccc                                                          <  2:3008822/101‑1 (MQ=17)
                                              ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCcg                                                         <  2:1524554/101‑1 (MQ=17)
                                              ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCcg                                                         >  1:1411281/1‑101 (MQ=17)
                                               gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgc                                                        <  1:76569/101‑2 (MQ=0)
                                               gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgc                                                        >  1:1383546/1‑100 (MQ=0)
                                               gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCcgg                                                        >  2:3035939/1‑101 (MQ=18)
                                                atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                       <  1:2038261/101‑3 (MQ=2)
                                                atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                       >  2:2911175/1‑99 (MQ=2)
                                                atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                       >  1:1063102/1‑99 (MQ=2)
                                                atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCA..GAGGGGGGC.CGCGCCCCCCCGCCCcggc                                                       >  1:1983187/1‑101 (MQ=14)
                                                  atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC..C.AAACGCCAAGAGGGGGAAGCG.CCCCCTGCCCcgccgg                                                     >  1:3675887/1‑101 (MQ=2)
                                                  atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                                                     >  2:3916301/1‑101 (MQ=18)
                                                     tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                                                  >  1:3235319/1‑101 (MQ=17)
                                                      gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGCATCCC.CCCCCTGCCCCGGCGGTCCg                                                 <  2:3138149/101‑1 (MQ=18)
                                                        agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg                                               <  2:1717607/101‑1 (MQ=17)
                                                        agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg                                               <  1:181884/101‑1 (MQ=17)
                                                         gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTgg                                              <  2:1059460/101‑1 (MQ=17)
                                                          caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGGCCGTggg                                             >  1:143678/1‑100 (MQ=17)
                                                            aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                           >  2:625069/1‑101 (MQ=18)
                                                            aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                           <  1:2538613/101‑1 (MQ=17)
                                                            aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                           >  1:4224779/1‑101 (MQ=17)
                                                              aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGCAGCTC.CCCCCTGCCCCGGCGGTCCGTGgcggcg                                         >  1:2845295/1‑101 (MQ=18)
                                                                gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TAGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                       >  1:335947/1‑101 (MQ=17)
                                                                 aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCt                                      <  1:3680930/101‑1 (MQ=17)
                                                                 aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGGCCGGGGCGGCGGCt                                      >  1:2207444/1‑101 (MQ=9)
                                                                     cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                  <  1:882183/101‑1 (MQ=17)
                                                                     cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCA..GAGGGGGGCAGGGC.CCCCCTGCCCCGGCGGGCCGCGGCGGCGCCCTGAc                                  >  1:3622924/1‑101 (MQ=17)
                                                                       gATGTCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCG..GAGGGGGGCAGGGC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gg                               <  2:1353657/101‑1 (MQ=18)
                                                                       gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gg                               <  2:2298375/101‑1 (MQ=17)
                                                                        aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.ggg                              <  1:4122895/101‑1 (MQ=17)
                                                                         tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggc                             >  2:517161/1‑101 (MQ=17)
                                                                         tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggc                             <  2:1784540/101‑1 (MQ=17)
                                                                          gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggcg                            <  2:2046365/101‑1 (MQ=17)
                                                                           ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggcgg                           <  1:1165216/101‑1 (MQ=17)
                                                                            cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggcggg                          >  2:3396601/1‑101 (MQ=18)
                                                                             tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCgggg                         >  2:3468148/1‑101 (MQ=18)
                                                                               tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGgc                       >  2:1293961/1‑101 (MQ=18)
                                                                                  tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGGC.GGGCGGGGGcgcg                    >  2:1136849/1‑101 (MQ=18)
                                                                                    ttGCCAGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGGCTTGAC.GGGCGGGGGGGCggg                  >  1:43816/1‑101 (MQ=17)
                                                                                     tGCCCGAAATATGCGGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGc                 <  2:2602539/101‑1 (MQ=18)
                                                                                     tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGc                 <  1:734103/101‑1 (MQ=18)
                                                                                       cccGAAATATCCCGGGGGGTGAA.TGAC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCAt               >  2:65134/1‑101 (MQ=18)
                                                                                        ccGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCAtt              <  1:321413/101‑1 (MQ=18)
                                                                                         cGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTa             <  2:262806/101‑1 (MQ=18)
                                                                                          gAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTaa            <  1:2176365/101‑1 (MQ=18)
                                                                                           aagtgtCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAAc           <  1:3190945/96‑1 (MQ=18)
                                                                                             atatCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCg         <  2:1554652/101‑1 (MQ=21)
                                                                                              tatCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGa        >  1:3055170/1‑101 (MQ=21)
                                                                                               atCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGG.GAGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTCGAC.GGGGGGGGGCGCGGGCAGTAACCCaa       >  1:640583/1‑101 (MQ=12)
                                                                                                tCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGaaa      >  2:2713553/1‑101 (MQ=21)
                                                                                                tCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGaaa      >  1:1570566/1‑101 (MQ=21)
                                                                                                 cccGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAAt     <  2:2775235/101‑1 (MQ=21)
                                                                                                    ggggggTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGc  >  1:1912598/1‑101 (MQ=25)
                                                                                                    ggggggTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGAGGTCCGAGGGGGGGCCTTGACTGGGCGGGGG.GCGGGCGTTAACGGaagtggc  >  1:1608576/1‑96 (MQ=2)
                                                                                                    ggggggCGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCCTTAACCGAAATTGc  >  2:1282704/1‑101 (MQ=21)
                                                                                                    |                                                                                                           
AGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGC  >  NC_014034/2493948‑2494146

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG