Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,551,310 Δ1 bp 100% intergenic (‑45/+124) RCAP_rcc02379 ← / ← ftsI CobW/HypB/UreG family cobalamin biosynthesis protein/peptidoglycan synthetase FtsI

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,551,3100A→.100.0% 562.5 130intergenic (‑45/+124)RCAP_rcc02379/ftsICobW/HypB/UreG family cobalamin biosynthesis protein/peptidoglycan synthetase FtsI
Reads supporting (aligned to +/- strand):  new base (71/57):  ref base (1/0):  total (73/57)

Only 100 of 145 total aligned reads displayed.

AGAAAGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGAAAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGCC  >  NC_014034/2551210‑2551404
                                                                                                    |                                                                                              
tgAAAGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGa                                                                                                >  2:2324624/2‑101 (MQ=255)
aGAAAGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGa                                                                                                <  2:3769890/101‑1 (MQ=255)
 gAAAGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaa                                                                                               <  1:1646594/101‑1 (MQ=255)
  aaaGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaag                                                                                              <  1:3862565/101‑2 (MQ=255)
   aaGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaagc                                                                                             >  2:498948/1‑99 (MQ=255)
   aaGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaagc                                                                                             >  1:1723317/1‑99 (MQ=255)
    aGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaagcc                                                                                            <  2:666245/101‑4 (MQ=255)
     gCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaagccc                                                                                           >  1:3951944/1‑97 (MQ=255)
     gCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaagccc                                                                                           <  1:3531064/101‑5 (MQ=255)
        cGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAgcccgcc                                                                                       <  2:1389745/101‑1 (MQ=255)
        cGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAgcccgcc                                                                                       <  1:3500163/101‑1 (MQ=255)
         gAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAgcccgccc                                                                                      >  1:259265/1‑101 (MQ=255)
          aaaGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGcccc                                                                                     >  2:3178233/1‑101 (MQ=255)
           aaGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGccccc                                                                                    <  2:3392925/101‑1 (MQ=255)
            aGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGcccccc                                                                                   <  2:1037958/101‑1 (MQ=255)
              gaCGCTGACCGGAAGGCGGGGATAAGTGGAAAAAAGGGTCATGGCCATCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGccccgaca                                                                                 <  2:1082799/100‑5 (MQ=255)
              cACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCg                                                                                 <  2:4199669/101‑1 (MQ=255)
              cACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCg                                                                                 <  2:288091/101‑1 (MQ=255)
               aCGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGc                                                                                >  2:2495142/1‑101 (MQ=255)
                   tGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGc                                                                            <  1:499005/101‑1 (MQ=255)
                   tGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGc                                                                            <  1:3181564/101‑1 (MQ=255)
                    gACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGcc                                                                           >  2:2687088/1‑101 (MQ=255)
                       cGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGc                                                                        <  2:2355119/101‑1 (MQ=255)
                        ggAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGcc                                                                       >  2:1686336/1‑101 (MQ=255)
                              cGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCcaaagca                                                                 >  2:4128404/1‑101 (MQ=255)
                               ggggATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCcaaagcaa                                                                >  1:3715945/1‑101 (MQ=255)
                                gggATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCcaaagcaaa                                                               <  1:2560385/101‑1 (MQ=255)
                                gggATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCcaaagcaaa                                                               >  2:3377544/1‑101 (MQ=255)
                                gggATCAGTGGGAGAAAGGGCCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCcaaagcaaa                                                               >  2:134359/1‑101 (MQ=255)
                                 ggATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCcaaagcaaag                                                              <  1:431100/101‑1 (MQ=255)
                                  gATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAgg                                                             <  1:3178472/101‑1 (MQ=255)
                                    tCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGc                                                           >  2:4008744/1‑101 (MQ=255)
                                    tCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGc                                                           <  2:2021252/101‑1 (MQ=255)
                                     cAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGcc                                                          >  2:534546/1‑101 (MQ=255)
                                      aGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCg                                                         >  2:1533553/1‑101 (MQ=255)
                                         gggAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGAc                                                      <  2:2725030/101‑1 (MQ=255)
                                            gatctgggTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCt                                                   <  2:750087/96‑1 (MQ=255)
                                            agaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCt                                                   <  2:1420487/101‑1 (MQ=255)
                                             gaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTg                                                  >  2:3539087/1‑101 (MQ=255)
                                             gaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTg                                                  >  1:2934672/1‑101 (MQ=255)
                                              aaaGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGc                                                 <  1:3822515/101‑1 (MQ=255)
                                              aaaGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGc                                                 >  2:3185484/1‑101 (MQ=255)
                                               aaGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCg                                                <  1:534610/101‑1 (MQ=255)
                                                aGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCgg                                               >  1:4197748/1‑101 (MQ=255)
                                                  ggTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGcc                                             >  1:881986/1‑101 (MQ=255)
                                                   gTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGccc                                            >  1:802043/1‑101 (MQ=255)
                                                   gTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGccc                                            <  1:2396337/101‑1 (MQ=255)
                                                   gTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCAGccc                                            <  2:259220/101‑1 (MQ=255)
                                                     cATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGa                                          >  2:1859744/1‑101 (MQ=255)
                                                       tGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAcc                                        <  2:2980455/101‑1 (MQ=255)
                                                       tGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAG.CCGGAAGCCCGCCCCCCCGCAAGCCATCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAcc                                        >  1:3619696/1‑101 (MQ=255)
                                                        ggCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccc                                       <  2:2247154/101‑1 (MQ=255)
                                                        ggCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccc                                       >  1:4069441/1‑101 (MQ=255)
                                                         gCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAcctc                                      <  1:2913587/101‑3 (MQ=255)
                                                         gCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAcccc                                      >  2:1622719/1‑101 (MQ=255)
                                                           cGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgt                                    <  2:3139556/101‑1 (MQ=255)
                                                              ccATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgtt                                 >  2:2506457/1‑101 (MQ=255)
                                                              ccATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgtt                                 >  2:1924010/1‑101 (MQ=255)
                                                              ccATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCACCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgtt                                 <  1:1273719/101‑1 (MQ=255)
                                                                aTCTGAGTTATGTTATACTATCACCCAAATAGCCCGAAA.CCCCCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgtttt                               >  1:2811053/1‑101 (MQ=255)
                                                                aTCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgtttt                               >  2:1550502/1‑101 (MQ=255)
                                                                aTCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgtttt                               >  1:471773/1‑101 (MQ=255)
                                                                 tCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttc                              <  1:2458711/101‑1 (MQ=255)
                                                                 tCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttc                              <  1:1568965/101‑1 (MQ=255)
                                                                  cTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttcc                             >  1:1768033/1‑101 (MQ=255)
                                                                  cTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttcc                             >  1:3889789/1‑101 (MQ=255)
                                                                   tGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttccc                            >  1:2738019/1‑101 (MQ=255)
                                                                      gttatgttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttccccgt                         <  1:2474643/101‑1 (MQ=255)
                                                                      gttatgttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttccccgt                         >  2:2584552/1‑101 (MQ=255)
                                                                       ttatgttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttccccggg                        >  1:2082827/1‑99 (MQ=255)
                                                                        tatgttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttccccgtgt                       >  1:3807660/1‑101 (MQ=255)
                                                                         atgttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttccccgtgtt                      >  1:990697/1‑101 (MQ=255)
                                                                           gttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttccccgtgtttt                    >  2:2603557/1‑101 (MQ=255)
                                                                           gttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGAccccgtgttttccccgtgtttt                    <  2:3128214/101‑1 (MQ=255)
                                                                            ttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGttttt                   <  1:2495326/101‑1 (MQ=255)
                                                                            ttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGttttt                   >  1:619995/1‑101 (MQ=255)
                                                                            ttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGttttt                   >  2:2975172/1‑101 (MQ=255)
                                                                             tataCTATCCCCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTc                  <  1:3447537/101‑1 (MQ=255)
                                                                               taCTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGCCCCCGTGGTTTCCCCGTGTTTTTCCt                >  1:3335757/1‑101 (MQ=255)
                                                                               taCTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCt                >  1:4081858/1‑101 (MQ=255)
                                                                               taCTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCt                <  1:744846/101‑1 (MQ=255)
                                                                                aCTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTg               >  1:3285995/1‑101 (MQ=255)
                                                                                  tATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGt             <  2:55458/101‑1 (MQ=255)
                                                                                  tATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGt             >  1:853443/1‑101 (MQ=255)
                                                                                  tATCACCAAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGt             <  2:881905/101‑1 (MQ=255)
                                                                                   aTCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTggcg            >  2:3271087/1‑99 (MQ=255)
                                                                                   aTCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTg            >  1:368370/1‑101 (MQ=255)
                                                                                      ccccAAATAGCCCG.AAGCCCCCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCaa         <  2:4162925/100‑1 (MQ=255)
                                                                                       cccAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCGCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCaaa        >  1:3613251/1‑101 (MQ=255)
                                                                                       cccAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCaaa        >  2:2874390/1‑101 (MQ=255)
                                                                                       cccAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCaaa        >  2:1330210/1‑101 (MQ=255)
                                                                                       cccAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCaaa        <  1:503631/101‑1 (MQ=255)
                                                                                        ccAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAAt       <  2:990606/101‑1 (MQ=255)
                                                                                        ccAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAAt       <  2:3951684/101‑1 (MQ=255)
                                                                                        ccAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAAt       <  2:3619443/101‑1 (MQ=255)
                                                                                        ccAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAAt       >  2:2271315/1‑101 (MQ=255)
                                                                                         cAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATa      >  1:1111906/1‑101 (MQ=255)
                                                                                          aaaTAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATAc     >  1:1478942/1‑101 (MQ=255)
                                                                                            aTAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGc   >  1:787329/1‑101 (MQ=255)
                                                                                             tAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGcc  <  1:3989090/101‑1 (MQ=255)
                                                                                                    |                                                                                              
AGAAAGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGAAAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGACCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGCC  >  NC_014034/2551210‑2551404

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG