Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,201 Δ1 bp 100% intergenic (‑177/+99) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2010A→.100.0% 86.8 124intergenic (‑177/+99)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (53/50):  ref base (14/7):  total (67/57)
Fisher's exact test strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.05e-03

Only 100 of 137 total aligned reads displayed.

AGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTTCGGCCAATT.CGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCC  >  NC_014034/2640102‑2640296
                                                                                                      |                                                                                               
 ggggttccacccGTGGCACTTTTGCCAAGGC.AAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                               <  2:3804816/91‑1 (MQ=17)
 ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                                <  1:1203772/101‑1 (MQ=25)
   cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                                                              <  1:1732739/101‑2 (MQ=25)
      gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                                                           <  2:4056931/101‑5 (MQ=21)
         aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                       <  2:2487968/101‑1 (MQ=21)
          aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                      <  1:3180511/101‑1 (MQ=25)
           ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCTGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                                     <  2:3662767/101‑1 (MQ=25)
                gcgcTTCTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                                <  1:685582/101‑1 (MQ=21)
                    ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                            <  2:3042280/101‑1 (MQ=25)
                       tGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCt                                                                         <  1:3837973/101‑1 (MQ=21)
                        gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                        <  1:251102/101‑1 (MQ=25)
                         cgcAGGCAAAAGGGGGGGGCGGCCCCCCCCTTTGGCCTTCGGCCAATTACCCCCCCCGGGGT.TTTGG.GGAAGAAGAAAAAACAAGGGGTT.ATCTTTCCTCa                                                                       >  2:1067304/1‑101 (MQ=255)
                         cgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                                       <  1:925952/101‑1 (MQ=25)
                           gccccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                                    >  1:1398393/7‑101 (MQ=14)
                           cAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaaa                                                                     <  2:1433926/101‑1 (MQ=25)
                            ccccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGCGAAAGGA..TGGGGTTTATCTTGCCCCAAata                                                                   >  2:723395/6‑101 (MQ=9)
                             cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                                  <  1:536724/97‑1 (MQ=17)
                             cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGGGACATTTGCGGCAAGGTGAAAGGA..TGGGGCTTATCTTGCCCCAAatat                                                                  >  2:1809181/5‑101 (MQ=9)
                              gCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                                  <  2:1115952/101‑1 (MQ=25)
                              gCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                                  <  2:3949620/101‑1 (MQ=25)
                               cAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                                 <  1:4044003/101‑1 (MQ=25)
                               cAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                                 >  2:3948178/1‑101 (MQ=21)
                                gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                               >  1:2592976/2‑101 (MQ=17)
                                 aaaGGGGGGAGCTGCCCCCCTC.TTGGCCTTTGGCCAATT.CACCCCCCGCGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATacc                                                               >  2:551273/1‑101 (MQ=18)
                                 aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCg                                                              >  1:131524/1‑101 (MQ=17)
                                   aagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                            >  2:1863998/3‑101 (MQ=18)
                                    ggggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                            <  2:211406/101‑1 (MQ=25)
                                    ggggggCCCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                            <  2:2338473/101‑1 (MQ=25)
                                    agggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                           >  2:4098878/2‑101 (MQ=18)
                                     gggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                          <  1:1490286/101‑1 (MQ=18)
                                     gggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                          >  2:1643891/1‑101 (MQ=18)
                                     gggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                                                           <  2:1443836/101‑1 (MQ=25)
                                      ggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                                         >  2:3483856/1‑100 (MQ=18)
                                      ggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggg                                                         >  2:2878552/1‑101 (MQ=18)
                                       gggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                         <  1:3920565/101‑1 (MQ=25)
                                        ggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                       <  1:439035/101‑4 (MQ=21)
                                        ggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                        <  2:2075963/101‑1 (MQ=25)
                                         gcgcTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                      <  2:3270389/101‑5 (MQ=21)
                                          cgcTGCCCCCCTC.TTGGCCTTCGGCCCATT.CACCCCCCCAGGTATTTTGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCCCAAATATCCCcgggggggg                                                      >  1:3457657/1‑98 (MQ=21)
                                          cgcTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGt                                                      <  2:3468420/101‑1 (MQ=25)
                                           gcTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                                    >  2:3670734/1‑95 (MQ=21)
                                           gcTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGNGNGtn                                                     >  1:1363739/1‑100 (MQ=25)
                                            cTGCCCCCCTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                    >  2:236615/1‑101 (MQ=25)
                                              gCCCCCCTC..TGG.CTTC..CCCATT.CCCCCCCCGGGGTATTTCGGGCAAGATGAAAGGCATCCGGTTTCATCTTGCTGCAAATATCCCGGGGGGAGTccgcttg                                               <  1:3647441/101‑6 (MQ=17)
                                                cccccTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                >  1:313449/1‑101 (MQ=37)
                                                cccccTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                >  2:3092790/1‑101 (MQ=37)
                                                 ccccTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                               <  1:3978114/101‑1 (MQ=37)
                                                  tcttc.tTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                              <  1:337064/98‑1 (MQ=25)
                                                  cccTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                              >  1:1208728/1‑101 (MQ=37)
                                                  cccTC.TTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                              >  1:1352283/1‑101 (MQ=37)
                                                    ctc.tTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                            >  1:3750789/1‑101 (MQ=37)
                                                     tc.tTGGCCTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                           >  1:2479405/1‑101 (MQ=37)
                                                     tc.tTGGCCTTCGGCCAATT.CAACCCCCGAGGAATTTGGAGCAAGATG.AAAGACCATGGGTTAATCTTGAATCAAATATCCCGGGGGGAGTCCTGAAAgaga                                           >  2:337013/1‑98 (MQ=25)
                                                        ttggccTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                         >  1:144686/6‑101 (MQ=37)
                                                         tggccTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                        >  1:3496281/5‑101 (MQ=37)
                                                         tggccTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACagg                                        >  2:3866125/5‑101 (MQ=25)
                                                         tggccTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGAAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                        >  1:4197583/5‑101 (MQ=37)
                                                          ggccTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                       <  1:1280080/98‑1 (MQ=37)
                                                           gtcTTCGTCCAATT.CCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                      <  1:2980666/99‑1 (MQ=37)
                                                            ccTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                     >  2:468205/2‑101 (MQ=37)
                                                             ctgcGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCGTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                    <  1:886318/98‑1 (MQ=37)
                                                             cTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                    >  2:1764107/1‑101 (MQ=37)
                                                              ttCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                                   >  2:4017796/1‑101 (MQ=37)
                                                                cGGCCAATT.CCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                 <  1:1922506/101‑1 (MQ=255)
                                                                cGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                 >  1:3003586/1‑101 (MQ=255)
                                                                cGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                 >  1:907509/1‑101 (MQ=37)
                                                                 ggCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                <  1:3158304/101‑1 (MQ=255)
                                                                 ggCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                >  1:2892209/1‑101 (MQ=37)
                                                                  gCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                               >  2:490034/1‑101 (MQ=37)
                                                                  gCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                               >  1:1952819/1‑101 (MQ=37)
                                                                    cAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                             >  2:1709144/1‑101 (MQ=255)
                                                                     aaTT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                            >  2:3597345/1‑101 (MQ=37)
                                                                          caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                        >  2:4042349/3‑101 (MQ=255)
                                                                          caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGAGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                        >  1:1174196/3‑101 (MQ=37)
                                                                           accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                       >  1:4120075/2‑101 (MQ=255)
                                                                            ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                      >  1:2928867/1‑101 (MQ=255)
                                                                             cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGCTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                     <  1:3620756/101‑1 (MQ=255)
                                                                              ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                    <  1:1709259/101‑1 (MQ=255)
                                                                              ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                    <  1:3093020/101‑1 (MQ=255)
                                                                               cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg                   >  2:2605875/1‑101 (MQ=255)
                                                                               cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg                   <  2:225005/101‑1 (MQ=255)
                                                                                  gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                >  1:2761305/1‑101 (MQ=255)
                                                                                  gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                >  2:2863901/1‑101 (MQ=255)
                                                                                  gAGGTAGTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                <  1:2254934/101‑1 (MQ=255)
                                                                                   aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt               >  1:2040531/1‑101 (MQ=255)
                                                                                   aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt               <  1:2793907/101‑1 (MQ=255)
                                                                                    ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt              <  2:2878654/101‑1 (MQ=255)
                                                                                    ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt              >  2:1466929/1‑101 (MQ=255)
                                                                                       aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc           >  2:4159715/1‑101 (MQ=255)
                                                                                        tttGGAGCACGATG.AAAGACCATGGGATCATCTTGCCTCAAATAGCCCGGGGGAAGTCCTGAAAGGACGCGGTGCTGACCCCCCCACCCCACTCTTTTTCa          >  2:752225/1‑101 (MQ=16)
                                                                                         ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa         >  1:3191625/1‑101 (MQ=255)
                                                                                         ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa         >  1:966126/1‑101 (MQ=255)
                                                                                          tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg        <  1:2451474/101‑1 (MQ=255)
                                                                                           ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc       >  1:1490583/1‑101 (MQ=255)
                                                                                            gagcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg      >  2:1188532/4‑101 (MQ=255)
                                                                                             agcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGt     >  2:4107679/3‑101 (MQ=255)
                                                                                               cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc   >  2:1060009/1‑101 (MQ=255)
                                                                                               cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc   <  1:161938/101‑1 (MQ=255)
                                                                                               cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc   <  1:551337/101‑1 (MQ=255)
                                                                                                aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc  <  2:207554/101‑1 (MQ=255)
                                                                                                      |                                                                                               
AGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTTCGGCCAATT.CGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCC  >  NC_014034/2640102‑2640296

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG