Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,207 Δ1 bp 14.5% intergenic (‑183/+93) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2070C→.14.5% 61.3 ‑1.5 ‑0.0138intergenic (‑183/+93)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/6):  ref base (63/54):  total (77/61)
Fisher's exact test strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.78e-01

Only 100 of 145 total aligned reads displayed.

AACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG..CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAA  >  NC_014034/2640111‑2640305
                                                                                                  |                                                                                                  
aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                                <  2:2487968/101‑1 (MQ=21)
aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                                <  1:3137833/101‑1 (MQ=25)
 aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                               <  1:3180511/101‑1 (MQ=25)
  ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCTGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                                              <  2:3662767/101‑1 (MQ=25)
  ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                                              <  1:1451997/101‑1 (MQ=25)
       gcgcTTCTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                                         <  1:685582/101‑1 (MQ=21)
         gcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCt                                                                                       <  1:2672922/101‑1 (MQ=25)
          cTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCtt                                                                                      <  1:3089224/101‑1 (MQ=21)
           ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                                     <  2:3042280/101‑1 (MQ=25)
               gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                                 <  1:251102/101‑1 (MQ=25)
                cgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                                                <  1:925952/101‑1 (MQ=25)
                  gccccgaaaAGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                                             >  1:1398393/7‑101 (MQ=14)
                  cAGGCAAAAGGGGGGGGCCGCCCCCCCCTTTGCCCTTGGGCCAATTACCCCCCCG.GGGATTTTGGGGAAAATGAAAA.ACCATGGGTTTATCTTTCCTCaaa                                                                              >  2:2451290/1‑101 (MQ=255)
                  cAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaaa                                                                              <  2:1433926/101‑1 (MQ=25)
                   ccccgaaaAGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGCGAAAGGA..TGGGGTTTATCTTGCCCCAAata                                                                            >  2:723395/6‑101 (MQ=9)
                    ggCAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                                            <  1:210318/101‑1 (MQ=21)
                    cccgaaaAGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                                           <  1:536724/97‑1 (MQ=17)
                    cccgaaaAGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGACATTTGCGGCAAGGTGAAAGGA..TGGGGCTTATCTTGCCCCAAatat                                                                           >  2:1809181/5‑101 (MQ=9)
                     gCAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                                           <  2:1115952/101‑1 (MQ=25)
                     gCAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                                           <  2:3949620/101‑1 (MQ=25)
                      cAAAAGGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                                          <  1:4044003/101‑1 (MQ=25)
                       gaaaAGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                        >  1:2592976/2‑101 (MQ=17)
                       gaaaAGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                        <  2:1086767/100‑1 (MQ=17)
                        aaaGGGGGGAGCTGCCCCCCTCTTGGC.CTTTGGCCAATTCACCCCCCGCGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATacc                                                                        >  2:551273/1‑101 (MQ=18)
                        aaaAGGGGGCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCg                                                                       >  1:131524/1‑101 (MQ=17)
                           ggggggCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                                     <  2:211406/101‑1 (MQ=25)
                           agggggCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                                    >  1:529732/2‑101 (MQ=18)
                           agggggCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                                    >  2:4098878/2‑101 (MQ=18)
                            gggggCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                                   >  2:1643891/1‑101 (MQ=18)
                            gggggCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                                   <  1:1490286/101‑1 (MQ=18)
                            gggggCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                                                                    <  2:1443836/101‑1 (MQ=25)
                             ggggCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                                                  >  2:3483856/1‑100 (MQ=18)
                             ggggCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggg                                                                  >  2:2878552/1‑101 (MQ=18)
                              gggCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggtg                                                                 >  1:4120356/1‑99 (MQ=18)
                              gggCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                                  <  1:3920565/101‑1 (MQ=25)
                               ggCGCTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                                <  1:439035/101‑4 (MQ=21)
                                gcgcTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                               <  2:3270389/101‑5 (MQ=21)
                                gcgcTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                                                <  2:3909766/101‑1 (MQ=25)
                                 cgcTGCCCCCCTCTTGGC.CTTCGGCCCATTCACCCCCCCAGGTATTTTGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCCCAAATATCCCcgggggggg                                                               >  1:3457657/1‑98 (MQ=21)
                                 cgcTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGt                                                               <  2:3468420/101‑1 (MQ=25)
                                  gcTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGNGNGtn                                                              >  1:1363739/1‑100 (MQ=25)
                                   cTGCCCCCCTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                             >  2:236615/1‑101 (MQ=25)
                                       cccccTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                         <  1:14955/101‑1 (MQ=25)
                                       cccccTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                         >  1:313449/1‑101 (MQ=37)
                                         cccTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                       >  1:1208728/1‑101 (MQ=37)
                                         cccTCTTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                       >  1:1352283/1‑101 (MQ=37)
                                           ctctTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                                     >  1:3750789/1‑101 (MQ=37)
                                           ctctTGGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                                     >  1:336164/1‑101 (MQ=37)
                                               ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                                  <  2:1208634/96‑1 (MQ=37)
                                                tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACagg                                                 >  2:3866125/5‑101 (MQ=25)
                                                 ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                                >  1:2898838/4‑101 (MQ=37)
                                                    ctgcGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCGTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                             <  1:886318/98‑1 (MQ=37)
                                                     ttCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                                            >  2:4017796/1‑101 (MQ=37)
                                                       cGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                          <  1:1922506/101‑1 (MQ=255)
                                                       cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                          >  1:3003586/1‑101 (MQ=255)
                                                       cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                          >  1:907509/1‑101 (MQ=37)
                                                         gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                        >  1:1952819/1‑101 (MQ=37)
                                                         gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                        >  2:490034/1‑101 (MQ=37)
                                                          ccAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                                       <  2:3002284/101‑1 (MQ=255)
                                                           cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                                      >  2:1709144/1‑101 (MQ=255)
                                                           cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                                      >  1:3660143/1‑101 (MQ=37)
                                                            aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                     >  2:3597345/1‑101 (MQ=37)
                                                             aTTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGCCCATGGGGTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                    <  2:463192/101‑1 (MQ=25)
                                                             aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                    >  1:2473321/1‑101 (MQ=37)
                                                               tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                  >  1:1213617/4‑101 (MQ=25)
                                                               tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                  <  2:3330312/98‑1 (MQ=255)
                                                                caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                 >  2:4042349/3‑101 (MQ=255)
                                                                caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                 >  2:1520481/3‑101 (MQ=255)
                                                                caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGAGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                 >  1:1174196/3‑101 (MQ=37)
                                                                 accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                >  1:4120075/2‑101 (MQ=255)
                                                                  ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                               >  1:2928867/1‑101 (MQ=255)
                                                                   cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGCTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                              <  1:3620756/101‑1 (MQ=255)
                                                                    ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                             <  1:1709259/101‑1 (MQ=255)
                                                                     cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg                            <  2:225005/101‑1 (MQ=255)
                                                                     cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg                            >  2:2605875/1‑101 (MQ=255)
                                                                       cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                          <  1:3948438/101‑1 (MQ=255)
                                                                        gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                         >  1:2761305/1‑101 (MQ=255)
                                                                        gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                         >  2:1804722/1‑101 (MQ=255)
                                                                        gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                         >  2:2863901/1‑101 (MQ=255)
                                                                         aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                        <  1:2793907/101‑1 (MQ=255)
                                                                             aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                    >  2:4159715/1‑101 (MQ=255)
                                                                              tttGGAGCACGATG.AAAGACCATGGGATCATCTTGCCTCAAATAGCCCGGGGGAAGTCCTGAAAGGACGCGGTGCTGACCCCCCCACCCCACTCTTTTTCa                   >  2:752225/1‑101 (MQ=16)
                                                                               ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                  >  1:966126/1‑101 (MQ=255)
                                                                               ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                  >  1:3191625/1‑101 (MQ=255)
                                                                                tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg                 <  1:2451474/101‑1 (MQ=255)
                                                                                  gagcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg               <  2:1952693/98‑1 (MQ=255)
                                                                                  gagcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg               >  2:1188532/4‑101 (MQ=255)
                                                                                     cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGTAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc            >  1:3591916/1‑101 (MQ=255)
                                                                                     cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc            <  1:161938/101‑1 (MQ=255)
                                                                                     cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc            >  2:1060009/1‑101 (MQ=255)
                                                                                      aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc           <  2:207554/101‑1 (MQ=255)
                                                                                          atgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc        >  2:210437/4‑101 (MQ=255)
                                                                                             aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa     >  2:3093698/1‑101 (MQ=255)
                                                                                             aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa     >  1:2226410/1‑101 (MQ=255)
                                                                                             aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa     >  1:432620/1‑101 (MQ=255)
                                                                                              aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg    >  1:3384745/1‑101 (MQ=255)
                                                                                              aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg    >  2:2331731/1‑101 (MQ=255)
                                                                                              aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg    >  2:2229677/1‑101 (MQ=255)
                                                                                               aGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGa   >  1:696715/1‑101 (MQ=255)
                                                                                               aGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGAGTTACGTCTCAGa   >  2:401716/1‑101 (MQ=255)
                                                                                                  |                                                                                                  
AACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG..CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAA  >  NC_014034/2640111‑2640305

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG