Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,725,840 Δ1 bp 6.4% intergenic (‑112/+85) cysT ← / ← cysK1 sulfate ABC transporter permease CysT/cysteine synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,725,8400C→. 6.4% 30.7 0.0 ‑0.2186intergenic (‑112/+85)cysT/cysK1sulfate ABC transporter permease CysT/cysteine synthase
Reads supporting (aligned to +/- strand):  new base (7/5):  ref base (100/74):  total (107/79)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.06e-01

Only 100 of 234 total aligned reads displayed.

TCCTTCCGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGGCGCCG  >  NC_014034/2725740‑2725940
                                                                                                     |                                                                                                    
tccttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.c                                                                                                      >  2:1288660/1‑101 (MQ=255)
 ccttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cc                                                                                                     <  1:3826292/101‑1 (MQ=255)
   ttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cccc                                                                                                   >  2:2777952/1‑101 (MQ=255)
    tccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.ccccc                                                                                                  <  2:1507431/101‑1 (MQ=255)
    tccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.ccccc                                                                                                  >  1:2111742/1‑101 (MQ=255)
     ccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cccccc                                                                                                 <  1:1903592/101‑1 (MQ=255)
     ccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cccccc                                                                                                 <  1:148810/101‑1 (MQ=255)
     ccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cccccc                                                                                                 >  2:2571461/1‑101 (MQ=255)
      cGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.ccccccc                                                                                                >  1:3536103/1‑101 (MQ=255)
        tGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.ccccccccc                                                                                              <  2:1664878/101‑1 (MQ=255)
        tGAGCTTCGGCATTTCCAAAATATCCACTGTGTCGGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.ccccccccc                                                                                              <  2:3736179/101‑1 (MQ=255)
         gAGCTTTGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAAACTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cccccccccc                                                                                             <  1:1027914/101‑1 (MQ=255)
          aGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCa                                                                                            <  1:1741002/101‑1 (MQ=255)
          aGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCa                                                                                            >  1:2856478/1‑101 (MQ=255)
           gCTTCGTCATTTCCAAAATATCCCCTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCAc                                                                                           <  1:2359118/101‑1 (MQ=255)
           gCTTCGTCATTTCCAAAATATCCACTGGGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCAc                                                                                           <  2:4136036/101‑1 (MQ=255)
            cTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.ccccccccccccc                                                                                          >  2:1819219/1‑98 (MQ=255)
            cTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACt                                                                                          >  1:3282370/1‑101 (MQ=255)
             ttCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT..CCCCCCCCCACTcc                                                                                        >  2:1001834/1‑101 (MQ=255)
                gTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTcccc                                                                                      >  2:1209786/1‑101 (MQ=255)
                 tCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTccccc                                                                                     <  1:1129699/101‑1 (MQ=255)
                 tCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTccccc                                                                                     >  1:4021709/1‑101 (MQ=255)
                  cATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCg                                                                                    <  1:2319949/101‑1 (MQ=255)
                  cATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCACCCCATTCCCCCg                                                                                    >  1:2302328/1‑101 (MQ=255)
                      tCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGcc                                                                                <  2:3169923/101‑1 (MQ=255)
                       attctatatCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCg                                                                               <  1:436385/96‑1 (MQ=255)
                        cAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCgg                                                                              <  1:841902/101‑1 (MQ=255)
                          aaaTATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCgggg                                                                            >  2:4097854/1‑101 (MQ=255)
                              atCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATATAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGcc                                                                        <  2:1553107/101‑1 (MQ=255)
                              atCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGCCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGGGcc                                                                        >  1:2775927/1‑101 (MQ=255)
                                 cACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGCGCCGGGGCGCCAGa                                                                     >  1:2133240/1‑101 (MQ=255)
                                   cTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT..CCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGa                                                                  >  1:1255569/1‑101 (MQ=255)
                                    tgtgTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCACACTCCCCCGTGCCGGGGCGCCAGACGa                                                                  >  2:497448/1‑101 (MQ=255)
                                     gtgtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGaa                                                                 <  1:741039/101‑1 (MQ=255)
                                      tgtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGaaa                                                                <  2:1525945/101‑1 (MQ=255)
                                      tgtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGaaa                                                                <  1:1832126/101‑1 (MQ=255)
                                       gtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGaaaa                                                               >  2:1126695/1‑101 (MQ=255)
                                       gtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGaaaa                                                               <  2:2349849/101‑1 (MQ=255)
                                         cAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAgc                                                             >  1:1554534/1‑100 (MQ=255)
                                          aGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGc                                                            >  2:3467472/1‑101 (MQ=255)
                                           gTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCCCTCCCCCGGCCCGGGGCGCCAGACGAAAAGGcc                                                           >  2:3312932/1‑101 (MQ=255)
                                            tCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGccc                                                          >  2:3369647/1‑101 (MQ=255)
                                             cGAGATTGAAAGGGATAAGTCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCTCGCGCCGGGGCCCCACACGAAAAGCcccc                                                         >  2:707428/1‑101 (MQ=255)
                                             cGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGcccc                                                         >  1:3680425/1‑101 (MQ=255)
                                              gagtTTGAAAGGGATAAATCTCGCCATTCTTGGGCGTGAATATAAAATTTTATT.CCCCCCCCCCCCTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCccg                                                        <  2:2663013/101‑1 (MQ=255)
                                                 aTTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCccgccg                                                     <  1:3312346/101‑1 (MQ=255)
                                                  ttGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCgg                                                    >  1:1291964/1‑101 (MQ=255)
                                                    gAAAGGGATAAATCTCGCCCTTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCgggg                                                  <  2:1416182/101‑1 (MQ=255)
                                                    gAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCgggg                                                  <  1:579268/101‑1 (MQ=255)
                                                     aaaGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCggggg                                                 >  1:1239756/1‑101 (MQ=255)
                                                      aaGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCgggggg                                                <  1:1856945/101‑1 (MQ=255)
                                                       aGGGATAAAACTCCCCCTTTTTGGTCGGGAAAAAAAAATTTTAAT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGggggc                                               <  2:2238209/101‑1 (MQ=255)
                                                         ggATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGggggcgg                                             >  2:2959214/1‑101 (MQ=255)
                                                         ggATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT..CCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGggggcggg                                            <  1:3909860/101‑1 (MQ=255)
                                                           aTAAATCTCGCCATTCTTGGTCGTTAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGTCCCGCCGGggggcgggg                                           <  1:2432434/101‑1 (MQ=255)
                                                              aaTCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCt                                        <  1:3174428/101‑1 (MQ=255)
                                                              aaTCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCACCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCt                                        >  2:3968499/1‑101 (MQ=255)
                                                                tctcGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGGGGGGCCttt                                      >  1:351611/1‑101 (MQ=255)
                                                                tcccGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCttt                                      <  1:1522526/98‑1 (MQ=255)
                                                                 ctcGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTg                                     <  2:3523464/101‑1 (MQ=255)
                                                                 ctcGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTg                                     >  1:166275/1‑101 (MQ=255)
                                                                 ctcGCCATTCTGGGTCGTAAATATAGAATTTTATC.CCCCCCCCCCTCCCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTg                                     <  1:2495127/101‑1 (MQ=255)
                                                                  tcGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGa                                    >  1:654197/1‑101 (MQ=255)
                                                                  cccccctttttGGTCGTGAATATAAGATTTTTTT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGa                                    <  2:597710/92‑1 (MQ=255)
                                                                    cccATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGaaa                                  <  2:3798649/100‑1 (MQ=255)
                                                                     ccATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAg                                 >  2:446904/1‑101 (MQ=255)
                                                                     ccATTCTTGGTCGTGAATATAGAATTTTATC.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAg                                 <  1:2571652/101‑1 (MQ=255)
                                                                      cATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGGGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGa                                >  2:820317/1‑101 (MQ=255)
                                                                       aTTCTTGGTCGTGAATATAGAATTTTATT..CCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGAcg                              <  1:1001925/101‑1 (MQ=255)
                                                                           ttGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTCCCGGGGCGCCAGACGAAAAGACCCCGCCGGGGGGGGGGCCCTTTGaaactcccgg                           >  1:4172096/1‑94 (MQ=255)
                                                                            tGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCggg                          <  1:3215600/101‑1 (MQ=255)
                                                                              gTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTCCCGGGGGGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAAACGCGGGGa                        >  1:2591651/1‑101 (MQ=255)
                                                                              gTCGTGAATATAGAATTTTATT..CCCCCCCCCACTCCCCCCTGCCCGGGCGCCCGACGAAAAGGCCCCGCCGGGGGGCGGGGCCCTTTAAAGACGCGGGGaa                       >  2:327072/1‑101 (MQ=255)
                                                                               tCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGaa                       <  2:2527968/101‑1 (MQ=255)
                                                                                cGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGaaa                      <  1:707505/101‑1 (MQ=255)
                                                                                cGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGaaa                      >  2:2484997/1‑101 (MQ=255)
                                                                                cGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGaaa                      >  2:2897575/1‑101 (MQ=255)
                                                                                 gTGAATATAGAATTTTATC.CCCCCCCCCCCCCCCCCCGGGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAAc                     <  1:3603843/101‑1 (MQ=255)
                                                                                  tGAATATAGAATTTTATTCCCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGAAGAAAAGGCCCCCCCGGGGGGGGGGGGCCTTGAAAGAGCCGGGGAAAc                     >  2:3594809/1‑101 (MQ=255)
                                                                                  tGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAAcc                    >  2:2165836/1‑101 (MQ=255)
                                                                                   gAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCg                   >  1:2884757/1‑101 (MQ=255)
                                                                                     atatAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGAt                 >  1:1438292/1‑101 (MQ=255)
                                                                                      tataGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGGGGGGCCTTTGAAAGACGCGGGGAAACCCATc                >  2:1768558/1‑101 (MQ=255)
                                                                                      tataGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATc                <  2:230663/101‑1 (MQ=255)
                                                                                      tataGAATTTTATT..CCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCa               >  1:2982754/1‑101 (MQ=255)
                                                                                      aagaGAATTTTCTC.CCCCCCCCCCACCCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATc                <  2:3546370/98‑1 (MQ=255)
                                                                                       ataGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAAGCCCCGCCGCGGGGCGGGGCCTTTGAAACACGCGGGGaacacgaaaa               >  2:2735958/1‑93 (MQ=255)
                                                                                         aGAATTTTATT.CCCCCCCCCCACTCCCCCGGGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAACACCCGGGGAAACCGagacgg             >  1:474184/1‑96 (MQ=255)
                                                                                         aGAATTTTATC.CCGCCCCCCCCCTCCCCCGTGCTGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAgg             <  1:3638143/101‑1 (MQ=255)
                                                                                           aaattttct.CCCCCCCCCCCCTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGcc           <  2:1459773/93‑1 (MQ=255)
                                                                                           aaTTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGcc           <  2:654120/101‑1 (MQ=255)
                                                                                           aaTTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGcc           <  2:2039286/101‑1 (MQ=255)
                                                                                             ttttATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCgg         >  1:2046356/1‑101 (MQ=255)
                                                                                             ttttATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGAACAGGCCgg         >  2:786240/1‑101 (MQ=255)
                                                                                              tttATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGCAACCGCTCAGGCCggg        >  2:3884190/1‑101 (MQ=255)
                                                                                                tATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGGGGGGAAACCGATCAGGACggggg      >  2:4176483/1‑100 (MQ=255)
                                                                                                tATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGgc      >  1:2722775/1‑101 (MQ=255)
                                                                                                  tt.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCCCCGGGGGGGGGGGCCTTTGAAAAACCGGGGGAAACCGATCAGGCCGGGgggc    >  2:2867609/1‑101 (MQ=255)
                                                                                                   tcccccccccccACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGgcgc    <  2:3282129/99‑1 (MQ=255)
                                                                                                     ccccccccccACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGGCGCCg  >  2:2365475/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
TCCTTCCGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGGCGCCG  >  NC_014034/2725740‑2725940

Base quality scores:  ATCG  < 3 ≤  ATCG  < 13 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 41 ≤  ATCG